DK0460426T3 - Fremgangsmåde til proteinrensning - Google Patents

Fremgangsmåde til proteinrensning

Info

Publication number
DK0460426T3
DK0460426T3 DK91107661.0T DK91107661T DK0460426T3 DK 0460426 T3 DK0460426 T3 DK 0460426T3 DK 91107661 T DK91107661 T DK 91107661T DK 0460426 T3 DK0460426 T3 DK 0460426T3
Authority
DK
Denmark
Prior art keywords
protein
antibodies
protein purification
ion exchange
solution
Prior art date
Application number
DK91107661.0T
Other languages
Danish (da)
English (en)
Inventor
Michael H Coan
Vivian W Lee
Original Assignee
Bayer Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24106028&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK0460426(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Bayer Ag filed Critical Bayer Ag
Application granted granted Critical
Publication of DK0460426T3 publication Critical patent/DK0460426T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
DK91107661.0T 1990-05-25 1991-05-11 Fremgangsmåde til proteinrensning DK0460426T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/528,523 US5110913A (en) 1990-05-25 1990-05-25 Antibody purification method

Publications (1)

Publication Number Publication Date
DK0460426T3 true DK0460426T3 (da) 1998-05-04

Family

ID=24106028

Family Applications (1)

Application Number Title Priority Date Filing Date
DK91107661.0T DK0460426T3 (da) 1990-05-25 1991-05-11 Fremgangsmåde til proteinrensning

Country Status (9)

Country Link
US (1) US5110913A (fr)
EP (1) EP0460426B1 (fr)
JP (1) JP3262128B2 (fr)
AT (1) ATE158302T1 (fr)
CA (1) CA2042945C (fr)
DE (1) DE69127657T2 (fr)
DK (1) DK0460426T3 (fr)
ES (1) ES2106742T3 (fr)
GR (1) GR3025504T3 (fr)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2163032C (fr) * 1993-06-03 2001-02-06 John Landon Fragments d'anticorps a des fins therapeutiques
US5759864A (en) * 1995-06-23 1998-06-02 Cedars Sinai Medical Center Methods for reducing background binding in antibody preparations
TW505655B (en) * 1997-10-14 2002-10-11 Tanox Inc Enhanced aggregate removal from bulk-biologicals using ion exchange chromatography
EP1308455B9 (fr) * 1998-05-06 2006-06-14 Genentech, Inc. Composition d'anticorps anti-HER2
ATE370961T1 (de) 1998-05-06 2007-09-15 Genentech Inc Reinigung von antikörpern durch ionenaustauschchromatographie
ES2228052T3 (es) 1998-06-01 2005-04-01 Genentech, Inc. Separacion demonomeros de anticuerpos de sus multimeros utilizando cromatografia de intercambio de iones.
JP4754219B2 (ja) * 2002-12-02 2011-08-24 アムジエン・フレモント・インコーポレイテツド 腫瘍壊死因子を対象とする抗体、およびそれらの使用
EP1963367A4 (fr) * 2005-12-06 2009-05-13 Amgen Inc Etapes de polissage dans des traitements de purification de proteines a etapes multiples
CN102391358B (zh) 2006-04-05 2016-08-10 艾伯维生物技术有限公司 抗体纯化
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
TW201516149A (zh) 2006-09-13 2015-05-01 Abbvie Inc 細胞培養改良
TWI442965B (zh) * 2006-11-01 2014-07-01 Biogen Idec Inc 使用低ｐＨ及二價陽離子分離生物巨分子的方法
HUE024877T2 (en) 2007-10-30 2016-02-29 Genentech Inc Antibody purification by cation exchange chromatography
CN102065870B (zh) * 2008-04-16 2014-11-19 比奥根艾迪克Ma公司 使用聚亚烷基二醇和过渡金属分离生物大分子的方法
CA2932207A1 (fr) * 2008-10-20 2010-12-09 Abbvie Inc. Isolation et purification d'anticorps au moyen de chromatographie d'affinite de proteine a
CN104974251A (zh) 2008-10-20 2015-10-14 Abbvie公司 在抗体纯化过程中的病毒灭活
WO2012149197A2 (fr) 2011-04-27 2012-11-01 Abbott Laboratories Procédé de contrôle du profil de galactosylation de protéines exprimées de manière recombinante
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
US9150645B2 (en) 2012-04-20 2015-10-06 Abbvie, Inc. Cell culture methods to reduce acidic species
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US9249182B2 (en) 2012-05-24 2016-02-02 Abbvie, Inc. Purification of antibodies using hydrophobic interaction chromatography
HK1211981A1 (en) 2012-09-02 2016-06-03 Abbvie Inc. Methods to control protein heterogeneity
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
SG11201507230PA (en) 2013-03-12 2015-10-29 Abbvie Inc Human antibodies that bind human tnf-alpha and methods of preparing the same
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
WO2014159554A1 (fr) * 2013-03-14 2014-10-02 Abbvie Inc. Composition d'espèces faiblement acides et leurs méthodes de production utilisant la chromatographie de déplacement
WO2014159579A1 (fr) 2013-03-14 2014-10-02 Abbvie Inc. Anticorps anti-tnfα ayant mutés et leurs procédés d'utilisation
WO2014151878A2 (fr) 2013-03-14 2014-09-25 Abbvie Inc. Procédés pour la modulation des profils de glycosylation de protéines de traitements à base de protéines recombinantes au moyen de monosaccharides et d'oligosaccharides
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
CN105315369B (zh) * 2014-07-25 2020-03-13 山东博安生物技术有限公司 利用阳离子交换层析纯化蛋白质

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6019733B2 (ja) * 1977-06-10 1985-05-17 科研製薬株式会社 蛋白質性活性物質
DE3526096A1 (de) * 1985-07-22 1987-01-22 Basf Ag Verfahren zur reinigung von htnf
AT399095B (de) * 1986-03-27 1995-03-27 Vukovich Thomas Dr Verfahren zur auftrennung von proteinen mittels gradientenelution und vorrichtung zur durchführung des verfahrens
US4771128A (en) * 1986-10-10 1988-09-13 Cetus Corporation Method of purifying toxin conjugates using hydrophobic interaction chromatography
ATE121418T1 (de) * 1987-10-23 1995-05-15 Schering Corp Verfahren zur proteinreinigung.
JPH01247096A (ja) * 1988-03-30 1989-10-02 Mitsubishi Petrochem Co Ltd モノクローナル抗体の回収方法

Also Published As

Publication number Publication date
EP0460426A3 (en) 1992-07-22
DE69127657T2 (de) 1998-01-15
EP0460426B1 (fr) 1997-09-17
JPH06316593A (ja) 1994-11-15
DE69127657D1 (de) 1997-10-23
ES2106742T3 (es) 1997-11-16
GR3025504T3 (en) 1998-02-27
US5110913A (en) 1992-05-05
JP3262128B2 (ja) 2002-03-04
CA2042945C (fr) 2004-05-18
CA2042945A1 (fr) 1991-11-26
EP0460426A2 (fr) 1991-12-11
ATE158302T1 (de) 1997-10-15

Similar Documents

Publication Publication Date Title
ATE158302T1 (de) Proteinreinigungsmethode
Porath Salt-promoted adsorption: recent developments
Porath Cross-linked dextrans as molecular sieves
Belew et al. High-performance analytical applications of immobilized metal ion affinity chromatography
ATE129347T1 (de) Biologisch aktive reagenzien aus carboxylgruppenhaltigen polymerisaten, analytisches element und verfahren zur verwendung.
ATE303434T1 (de) Effiziente reinigung von adenovirus
ATE179425T1 (de) Verfahren zur abreicherung oder entfernung von endotoxinen
ES2081742A1 (es) Procedimiento para preparar inmunoglobulina purificada.
DE68915675D1 (de) Verfahren zur Reinigung von Vitamin K-abhängigen Proteinen.
PT1308455E (pt) Composicao compreendendo anticorpos anti-her2
DK564989A (da) Bindingsproteiner
GB1447074A (en) Method for separating a polypeptide from microorganisms
ATE124703T1 (de) Verfahren zur trennung von proteinen.
ATE111519T1 (de) Familie von modifizierten antikörpern mit hoher affinität zur behandlung von krebs.
DE50213370D1 (de) Mikrofluidisches extraktionsverfahren
DE69012734D1 (de) Verfahren zum Eluieren von adsorbiertem Gold aus Kohlenstoff.
CA2213577A1 (fr) Procede de preparation du facteur ix a partir de sources biologiques
Ramadan et al. α-Aminoacyl hydroxamate adsorbents—a new type of immobilized chelator
DK510084D0 (da) Uoploeselige overflader behandlet til haemning af ikke-specifik proteinbinding
RU94023134A (ru) Способ выделения и очистки инсулина и его биотехнологических предшественников
SE9201976D0 (sv) En metod foer att adsorbera proteiner och peptider till hydrofoba zeoliter
DE69015056D1 (de) Trennung von Antikörpern gegen Metallchelate.
ZA903843B (en) Tumor necrosis factor binding protein ii,its purification and antibodies thereto
JPH03291300A (ja) ポリクローナル抗体の分離方法
SU1159579A1 (ru) Способ получени белкового антигена фасциол