DK0725816T3 - Fremgangsmåde til dyrkning af bipolært adhærerede hepatocyter - Google Patents
Fremgangsmåde til dyrkning af bipolært adhærerede hepatocyterInfo
- Publication number
- DK0725816T3 DK0725816T3 DK94930220T DK94930220T DK0725816T3 DK 0725816 T3 DK0725816 T3 DK 0725816T3 DK 94930220 T DK94930220 T DK 94930220T DK 94930220 T DK94930220 T DK 94930220T DK 0725816 T3 DK0725816 T3 DK 0725816T3
- Authority
- DK
- Denmark
- Prior art keywords
- matrix
- serum
- free culture
- bipolar
- added
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 210000003494 hepatocyte Anatomy 0.000 title abstract 2
- 230000001464 adherent effect Effects 0.000 title 1
- 238000012258 culturing Methods 0.000 title 1
- 239000011159 matrix material Substances 0.000 abstract 8
- 210000005229 liver cell Anatomy 0.000 abstract 2
- 102000012422 Collagen Type I Human genes 0.000 abstract 1
- 108010022452 Collagen Type I Proteins 0.000 abstract 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 abstract 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 abstract 1
- 102000016359 Fibronectins Human genes 0.000 abstract 1
- 108010067306 Fibronectins Proteins 0.000 abstract 1
- 230000005526 G1 to G0 transition Effects 0.000 abstract 1
- 210000002744 extracellular matrix Anatomy 0.000 abstract 1
- 230000003619 fibrillary effect Effects 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 230000007774 longterm Effects 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/067—Hepatocytes
- C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE4336399A DE4336399A1 (de) | 1993-10-26 | 1993-10-26 | Verfahren zur Verbesserung der Matrixbedingungen bipolar adhärierter Hepatozyten und zur Herstellung eines entsprechend konfigurierten Zellkulturkits |
| PCT/EP1994/003501 WO1995011963A1 (de) | 1993-10-26 | 1994-10-25 | Verfahren zum züchten von bipolar adhärierten hepatozyten |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK0725816T3 true DK0725816T3 (da) | 2000-07-31 |
Family
ID=6500981
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK94930220T DK0725816T3 (da) | 1993-10-26 | 1994-10-25 | Fremgangsmåde til dyrkning af bipolært adhærerede hepatocyter |
Country Status (11)
| Country | Link |
|---|---|
| EP (1) | EP0725816B1 (da) |
| JP (1) | JP2004537951A (da) |
| AT (1) | ATE190648T1 (da) |
| AU (1) | AU7940194A (da) |
| CA (1) | CA2174925A1 (da) |
| DE (2) | DE4336399A1 (da) |
| DK (1) | DK0725816T3 (da) |
| ES (1) | ES2145845T3 (da) |
| NO (1) | NO961653D0 (da) |
| PT (1) | PT725816E (da) |
| WO (1) | WO1995011963A1 (da) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996001426A1 (en) * | 1994-07-01 | 1996-01-18 | W.R. Grace & Co.-Conn. | Hepatocyte test kit |
| ATE429486T1 (de) | 2001-08-15 | 2009-05-15 | Takara Bio Inc | Expansionskulturverfahren für antigenspezifische zytotoxische t-lymphozyten |
| TW201043699A (en) | 2002-03-25 | 2010-12-16 | Takara Bio Inc | Process for producing cytotoxic lymphocyte |
| US8927273B2 (en) * | 2003-08-22 | 2015-01-06 | Takara Bio Inc. | Process for producing cytotoxic lymphocytes |
| WO2007020880A1 (ja) | 2005-08-17 | 2007-02-22 | Takara Bio Inc. | リンパ球の製造方法 |
| JP6454172B2 (ja) * | 2015-02-12 | 2019-01-16 | 日本バイリーン株式会社 | 肝細胞の培養方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4206585C2 (de) * | 1992-03-03 | 1994-11-24 | Augustinus Dr Med Bader | Vorrichtung zur Massenkultur von Zellen |
| DE4222345A1 (de) * | 1992-07-08 | 1994-01-13 | Augustinus Dr Med Bader | Verfahren zum Züchten einer Zellart im Kokulturverfahren mit Leberzellen |
-
1993
- 1993-10-26 DE DE4336399A patent/DE4336399A1/de not_active Withdrawn
-
1994
- 1994-10-25 ES ES94930220T patent/ES2145845T3/es not_active Expired - Lifetime
- 1994-10-25 EP EP94930220A patent/EP0725816B1/de not_active Expired - Lifetime
- 1994-10-25 AU AU79401/94A patent/AU7940194A/en not_active Abandoned
- 1994-10-25 PT PT94930220T patent/PT725816E/pt unknown
- 1994-10-25 AT AT94930220T patent/ATE190648T1/de not_active IP Right Cessation
- 1994-10-25 WO PCT/EP1994/003501 patent/WO1995011963A1/de not_active Ceased
- 1994-10-25 DE DE59409216T patent/DE59409216D1/de not_active Expired - Fee Related
- 1994-10-25 CA CA002174925A patent/CA2174925A1/en not_active Abandoned
- 1994-10-25 DK DK94930220T patent/DK0725816T3/da active
- 1994-10-25 JP JP51240695A patent/JP2004537951A/ja not_active Ceased
-
1996
- 1996-04-25 NO NO961653A patent/NO961653D0/no unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ES2145845T3 (es) | 2000-07-16 |
| CA2174925A1 (en) | 1995-05-04 |
| JP2004537951A (ja) | 2004-12-24 |
| PT725816E (pt) | 2000-08-31 |
| DE59409216D1 (de) | 2000-04-20 |
| NO961653L (no) | 1996-04-25 |
| NO961653D0 (no) | 1996-04-25 |
| AU7940194A (en) | 1995-05-22 |
| EP0725816B1 (de) | 2000-03-15 |
| ATE190648T1 (de) | 2000-04-15 |
| WO1995011963A1 (de) | 1995-05-04 |
| DE4336399A1 (de) | 1995-04-27 |
| EP0725816A1 (de) | 1996-08-14 |
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