DK1546328T3 - Cellelinier til heterolog ekspression af genprodukter i höjt udbytte og med humant glycosyleringsmönster - Google Patents

Cellelinier til heterolog ekspression af genprodukter i höjt udbytte og med humant glycosyleringsmönster

Info

Publication number
DK1546328T3
DK1546328T3 DK03776861T DK03776861T DK1546328T3 DK 1546328 T3 DK1546328 T3 DK 1546328T3 DK 03776861 T DK03776861 T DK 03776861T DK 03776861 T DK03776861 T DK 03776861T DK 1546328 T3 DK1546328 T3 DK 1546328T3
Authority
DK
Denmark
Prior art keywords
cell
gene product
starting
high yield
dna sequence
Prior art date
Application number
DK03776861T
Other languages
Danish (da)
English (en)
Inventor
Volker Sandig
Karsten Winkler
Uwe Marx
Tobias Wermelinger
Original Assignee
Probiogen Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Probiogen Ag filed Critical Probiogen Ag
Application granted granted Critical
Publication of DK1546328T3 publication Critical patent/DK1546328T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DK03776861T 2002-10-04 2003-10-06 Cellelinier til heterolog ekspression af genprodukter i höjt udbytte og med humant glycosyleringsmönster DK1546328T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP02022194A EP1405908A1 (de) 2002-10-04 2002-10-04 Herstellung von Zelllinien zur heterologen Expression

Publications (1)

Publication Number Publication Date
DK1546328T3 true DK1546328T3 (da) 2006-08-07

Family

ID=31985049

Family Applications (1)

Application Number Title Priority Date Filing Date
DK03776861T DK1546328T3 (da) 2002-10-04 2003-10-06 Cellelinier til heterolog ekspression af genprodukter i höjt udbytte og med humant glycosyleringsmönster

Country Status (9)

Country Link
US (1) US20060148085A1 (de)
EP (2) EP1405908A1 (de)
JP (1) JP2006500937A (de)
AT (1) ATE324439T1 (de)
AU (1) AU2003286135B2 (de)
CA (1) CA2501218A1 (de)
DE (1) DE60304881T2 (de)
DK (1) DK1546328T3 (de)
WO (1) WO2004031383A1 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104109712A (zh) 2004-02-18 2014-10-22 克罗莫塞尔公司 使用信号探针的方法和材料
CN101061214B (zh) * 2004-10-22 2012-12-05 诺维信公司 多个多核苷酸拷贝的稳定基因组整合
CA2716801A1 (en) * 2008-03-12 2009-09-17 Wyeth Llc Methods for identifying cells suitable for large-scale production of recombinant proteins
EP2105505A1 (de) 2008-03-28 2009-09-30 Celonic AG Verfahren und Materialien zur wiederholbaren Erzeugung hochproduktiver Zelllinien für rekombinante Proteine
US20110136236A1 (en) * 2008-04-21 2011-06-09 Unitargeting Research As Genetically modified eukaryotic cells
EP2527448A1 (de) 2011-05-23 2012-11-28 Novozymes A/S Gleichzeitige standortspezifische Integrationen von mehreren Genkopien in faserige Pilze
FR2981946B1 (fr) * 2011-10-28 2015-02-20 Lfb Biotechnologies Unites de transcription et leur utilisation dans des vecteurs d'expression (yb2/0)
WO2018077796A1 (en) 2016-10-25 2018-05-03 Novozymes A/S Flp-mediated genomic integrationin bacillus licheniformis
GB201703417D0 (en) 2017-03-03 2017-04-19 Ge Healthcare Bio Sciences Ab Method for cell line development
WO2023039463A1 (en) * 2021-09-09 2023-03-16 Bioconsortia, Inc. Blind editing of polynucleotide sequences
WO2024137877A1 (en) * 2022-12-21 2024-06-27 Clara Foods Co. Systems and methods for high yielding recombinant microorganisms and uses thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6130364A (en) * 1995-03-29 2000-10-10 Abgenix, Inc. Production of antibodies using Cre-mediated site-specific recombination
US6086913A (en) * 1995-11-01 2000-07-11 University Of British Columbia Liposomal delivery of AAV vectors
US6534314B1 (en) * 1996-06-14 2003-03-18 Massachusetts Institute Of Technology Methods and compositions for transforming cells
US6262341B1 (en) * 1997-11-18 2001-07-17 Pioneer Hi-Bred International, Inc. Method for the integration of foreign DNA into eukaryotic genomes
CN1455817A (zh) * 2000-07-21 2003-11-12 (由农业部部长代表的)美利坚合众国 用于在真核基因组中置换、转移和富积dna的方法

Also Published As

Publication number Publication date
ATE324439T1 (de) 2006-05-15
WO2004031383A1 (en) 2004-04-15
AU2003286135A1 (en) 2004-04-23
AU2003286135B2 (en) 2009-01-15
CA2501218A1 (en) 2004-04-15
EP1546328B1 (de) 2006-04-26
EP1405908A1 (de) 2004-04-07
JP2006500937A (ja) 2006-01-12
US20060148085A1 (en) 2006-07-06
EP1546328A1 (de) 2005-06-29
DE60304881D1 (de) 2006-06-01
DE60304881T2 (de) 2007-04-19

Similar Documents

Publication Publication Date Title
DK1546328T3 (da) Cellelinier til heterolog ekspression af genprodukter i höjt udbytte og med humant glycosyleringsmönster
DE69731834D1 (de) Agouti verwandtes gen
TW200510529A (en) Methods for the production of insulin in plants
DK1567652T3 (da) Nye neomycin-phosphotransferasegener og fremgangsmåde til selektion af höjtproducerende rekombinante celler
DE50301836D1 (de) Verfahren zur fermentativen Herstellung von Aminosäuren und Aminosäure-Derivaten der Phosphoglycerat-Familie
ATE466094T1 (de) Verfahren zur herstellung von oligosacchariden
WO2003018751A3 (en) Apparatus and method for electroporation of biological samples
DK1208205T3 (da) Fremgangsmåder til frembringelse af L-aminosyrer ved hjælp af en forögelse af cellulært NADPH
ATE423756T1 (de) Verfahren zur herstellung von hochreinem methyl- tert.-butylether
WO2009069381A1 (ja) フラビンアデニンジヌクレオチド結合型グルコース脱水素酵素遺伝子が導入された形質転換体、及び該形質転換体を用いたフラビンアデニンジヌクレオチド結合型グルコース脱水素酵素の製造法
NO20053357L (no) Dyrkingsmedium, dyrkingsmetode og de oppnadde myoblaster og deres anvendelse.
IL175021A0 (en) Chromatin insulator, its preparation and use in protein expression
ATE353953T1 (de) Wirtszelle, die reduzierte mengen einer metalloprotease exprimiert und methoden zur verwendung dieser wirtszelle zur proteinproduktion
AU2003238717A1 (en) POLYPEPTIDES HAVING Alpha- H-Alpha-AMINO ACID AMIDE RACEMASE ACTIVITY AND NUCLEIC ACIDS ENCODING THE SAME
ATE542913T1 (de) Promotorvarianten zur expression von genen in einer pilzzelle
WO2001075132A3 (en) Method for producing authentic cytokines in plants
WO2004046340A3 (en) Intron fusion construct and method of using for selecting high-expressing production cell lines
DE60326449D1 (de) Chimere human papilloma virus 16 l1 proteine, welche ein l2 peptid enthalten, damit hergestellte virus-ähnliche partikel und methode zur herstellung der partikel
WO2002098455A3 (en) Production of recombinant human arylsulfatase a
AU2003231862A1 (en) Methods and dna constructs for high yield production of polypeptides
DE60302776D1 (de) Verfahren zur rekombinanten Herstellung von Polypeptiden
WO2002088338A3 (fr) Procede de replication du virus de l'hepatite c
DE50013804D1 (de) VERFAHREN ZUM GEWINNEN VON REKOMBINANTEM HBsAg
WO2004027026A3 (en) Improved methods for preparing highly active april ligand polypeptides
ATE244709T1 (de) Verfahren zur herstellung von benzofuranonoximen