DK1576094T3 - Fremgangsmåder til at danne modificerede promotere resulterende i varierende genekspressionsniveauer - Google Patents

Fremgangsmåder til at danne modificerede promotere resulterende i varierende genekspressionsniveauer

Info

Publication number
DK1576094T3
DK1576094T3 DK03724099.1T DK03724099T DK1576094T3 DK 1576094 T3 DK1576094 T3 DK 1576094T3 DK 03724099 T DK03724099 T DK 03724099T DK 1576094 T3 DK1576094 T3 DK 1576094T3
Authority
DK
Denmark
Prior art keywords
gene expression
methods
varying levels
forming modified
modified promoters
Prior art date
Application number
DK03724099.1T
Other languages
English (en)
Inventor
Marguerite A Cervin
Fernando Valle
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=29254561&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK1576094(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Application granted granted Critical
Publication of DK1576094T3 publication Critical patent/DK1576094T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/06—Methods of screening libraries by measuring effects on living organisms, tissues or cells
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK03724099.1T 2002-04-22 2003-04-18 Fremgangsmåder til at danne modificerede promotere resulterende i varierende genekspressionsniveauer DK1576094T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US37473502P 2002-04-22 2002-04-22
US37462702P 2002-04-22 2002-04-22
PCT/US2003/012044 WO2003089604A2 (en) 2002-04-22 2003-04-18 Methods of creating modified promoters resulting in varying levels of gene expression

Publications (1)

Publication Number Publication Date
DK1576094T3 true DK1576094T3 (da) 2012-01-02

Family

ID=29254561

Family Applications (1)

Application Number Title Priority Date Filing Date
DK03724099.1T DK1576094T3 (da) 2002-04-22 2003-04-18 Fremgangsmåder til at danne modificerede promotere resulterende i varierende genekspressionsniveauer

Country Status (8)

Country Link
US (1) US20060057633A1 (da)
EP (1) EP1576094B1 (da)
JP (1) JP2006513692A (da)
AT (1) ATE526419T1 (da)
AU (1) AU2003230981A1 (da)
CA (2) CA2830864C (da)
DK (1) DK1576094T3 (da)
WO (1) WO2003089604A2 (da)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050079617A1 (en) 2003-10-03 2005-04-14 Cervin Marguerite A. Glucose transport mutants for production of biomaterial
EP2428573A3 (en) 2002-10-04 2012-06-27 Genecor International, Inc. Improved production of bacterial strains cross reference to related applications
CA2500741C (en) * 2002-10-04 2017-02-28 Genencor International, Inc. Glucose transport mutants for production of biomaterial
US20120315670A1 (en) * 2009-11-02 2012-12-13 Gen9, Inc. Compositions and Methods for the Regulation of Multiple Genes of Interest in a Cell
US20140273152A1 (en) * 2013-03-13 2014-09-18 Oregon Health & Science University Recombinant manganese oxidase
EP3585910B1 (en) 2017-02-24 2024-03-20 Danisco US Inc. Compositions and methods for increased protein production in bacillus licheniformis
FI3607097T3 (fi) 2017-04-07 2023-09-11 Dupont Nutrition Biosci Aps BEETA-GALAKTOSIDAASEJA JA LAKTAASEJA ILMAN p-NITROBENTSYYLIESTERASISIVUAKTIIVISUUTTA TUOTTAVIA BACILLUS-ISÄNTÄSOLUJA
US11879127B2 (en) 2017-08-23 2024-01-23 Danisco Us Inc. Methods and compositions for efficient genetic modifications of Bacillus licheniformis strains
US20210032639A1 (en) 2017-09-13 2021-02-04 Danisco Us Inc. Modified 5'-untranslated region (utr) sequences for increased protein production in bacillus
CN111670244B (zh) 2018-01-03 2023-08-01 丹尼斯科美国公司 突变的和遗传修饰的芽孢杆菌属细胞及其用于增加蛋白质产生的方法
AU2019309401A1 (en) * 2018-07-25 2021-02-11 Flagship Pioneering Innovations V, Inc. Compositions and related methods for agriculture
US11781147B2 (en) 2018-11-28 2023-10-10 Danisco Us Inc. Promoter sequences and methods thereof for enhanced protein production in Bacillus cells
CN110144626A (zh) * 2019-03-29 2019-08-20 浙江工业大学 一种启动子文库的构建方法
JP2022526982A (ja) 2019-04-05 2022-05-27 ダニスコ・ユーエス・インク 線状組換えＤＮＡコンストラクトを使用してバチルス（Ｂａｃｉｌｌｕｓ）のゲノムにドナーＤＮＡ配列を組み込むための方法及びその組成物
CA3136113A1 (en) 2019-04-05 2020-10-08 Danisco Us Inc. Methods for polynucleotide integration into the genome of bacillus using dual circular recombinant dna constructs and compositions thereof
KR102194368B1 (ko) * 2019-06-05 2020-12-24 서울시립대학교 산학협력단 미생물의 유전체를 개량하기 위한 변이 균주, 이의 제조방법 및 이를 이용한 미생물의 유전체 개량 방법
KR20220041928A (ko) 2019-08-14 2022-04-01 다니스코 유에스 인크. 바실러스 리체니포르미스에서 단백질 생산을 증가시키기 위한 조성물 및 방법
WO2021086606A1 (en) 2019-10-28 2021-05-06 Danisco Us Inc Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases
JP7656603B2 (ja) 2019-11-11 2025-04-03 ダニスコ・ユーエス・インク バチルス属（ｂａｃｉｌｌｕｓ）細胞における強化されたタンパク質産生のための組成物及び方法
WO2021101950A1 (en) 2019-11-19 2021-05-27 Danisco Us Inc Selection marker free methods for modifying the genome of bacillus and compositions thereof
JP7787077B2 (ja) 2020-01-15 2025-12-16 ダニスコ・ユーエス・インク バチルス・リケニフォルミス（ｂａｃｉｌｌｕｓ ｌｉｃｈｅｎｉｆｏｒｍｉｓ）における強化したタンパク質産生のための組成物及び方法
CN116897160A (zh) 2021-02-22 2023-10-17 丹尼斯科美国公司 在色素缺陷型芽孢杆菌属细胞中产生目的蛋白的方法和组合物
CN117769597A (zh) 2021-05-24 2024-03-26 丹尼斯科美国公司 用于增强芽孢杆菌属细胞中蛋白质产生的组合物和方法
KR20240099301A (ko) 2021-11-16 2024-06-28 다니스코 유에스 인크. 바실러스 세포에서 단백질 생산을 향상시키기 위한 조성물 및 방법
JPWO2023233996A1 (da) * 2022-05-31 2023-12-07
CN121889413A (zh) 2023-08-09 2026-04-17 丹尼斯科美国公司 用于增强革兰氏阳性细菌细胞中的蛋白质产生的组合物和方法
WO2026090396A1 (en) 2024-10-24 2026-04-30 Danisco Us Inc. Compositions and methods for enhanced protein production in gram‑positive bacterial cells

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5532142A (en) * 1993-02-12 1996-07-02 Board Of Regents, The University Of Texas System Method of isolation and purification of fusion polypeptides
WO1994025609A1 (en) * 1993-04-28 1994-11-10 Hybritech Incorporated Method for creating optimized regulatory regions affecting protein expression and protein trafficking
US6140087A (en) * 1993-06-24 2000-10-31 Advec, Inc. Adenovirus vectors for gene therapy
NZ500843A (en) * 1995-06-07 2002-03-28 Invitrogen Corp A method of DNA recombination which is selectable to select for cells containing a product and against cells only harbouring the insert donor
US7199233B1 (en) * 1996-08-23 2007-04-03 Peter Ruhdal Jensen Artificial promoter libraries for selected organisms and promoters derived from such libraries
US5851808A (en) * 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
US6294358B1 (en) * 1999-09-07 2001-09-25 Thermogen, Inc. Thermus promoters for gene expression

Also Published As

Publication number Publication date
ATE526419T1 (de) 2011-10-15
US20060057633A1 (en) 2006-03-16
WO2003089604A2 (en) 2003-10-30
CA2483006A1 (en) 2003-10-30
WO2003089604A3 (en) 2006-02-16
AU2003230981A8 (en) 2003-11-03
AU2003230981A1 (en) 2003-11-03
CA2830864A1 (en) 2003-10-30
EP1576094B1 (en) 2011-09-28
EP1576094A2 (en) 2005-09-21
JP2006513692A (ja) 2006-04-27
CA2483006C (en) 2013-10-29
EP1576094A4 (en) 2006-08-30
CA2830864C (en) 2017-03-21

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