ATE526419T1 - Verfahren zur erzeugung zu variierenden genexpressionsniveaus führender modifizierter promotoren - Google Patents
Verfahren zur erzeugung zu variierenden genexpressionsniveaus führender modifizierter promotorenInfo
- Publication number
- ATE526419T1 ATE526419T1 AT03724099T AT03724099T ATE526419T1 AT E526419 T1 ATE526419 T1 AT E526419T1 AT 03724099 T AT03724099 T AT 03724099T AT 03724099 T AT03724099 T AT 03724099T AT E526419 T1 ATE526419 T1 AT E526419T1
- Authority
- AT
- Austria
- Prior art keywords
- expression levels
- gene expression
- promotors
- leading
- modified
- Prior art date
Links
- 230000014509 gene expression Effects 0.000 title abstract 3
- 230000001580 bacterial effect Effects 0.000 abstract 3
- 239000002243 precursor Substances 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B30/00—Methods of screening libraries
- C40B30/06—Methods of screening libraries by measuring effects on living organisms, tissues or cells
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Virology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US37462702P | 2002-04-22 | 2002-04-22 | |
| US37473502P | 2002-04-22 | 2002-04-22 | |
| PCT/US2003/012044 WO2003089604A2 (en) | 2002-04-22 | 2003-04-18 | Methods of creating modified promoters resulting in varying levels of gene expression |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE526419T1 true ATE526419T1 (de) | 2011-10-15 |
Family
ID=29254561
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03724099T ATE526419T1 (de) | 2002-04-22 | 2003-04-18 | Verfahren zur erzeugung zu variierenden genexpressionsniveaus führender modifizierter promotoren |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20060057633A1 (de) |
| EP (1) | EP1576094B1 (de) |
| JP (1) | JP2006513692A (de) |
| AT (1) | ATE526419T1 (de) |
| AU (1) | AU2003230981A1 (de) |
| CA (2) | CA2830864C (de) |
| DK (1) | DK1576094T3 (de) |
| WO (1) | WO2003089604A2 (de) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050079617A1 (en) | 2003-10-03 | 2005-04-14 | Cervin Marguerite A. | Glucose transport mutants for production of biomaterial |
| EP1546304B1 (de) | 2002-10-04 | 2013-05-08 | Danisco US Inc. | VERBESSERTE PRODUKTION VON BAKTERIENSTûMMEN |
| KR101130587B1 (ko) * | 2002-10-04 | 2012-03-30 | 다니스코 유에스 인크. | 생물질 제조용 글루코오스 수송 돌연변이 |
| WO2011053957A2 (en) * | 2009-11-02 | 2011-05-05 | Gen9, Inc. | Compositions and methods for the regulation of multiple genes of interest in a cell |
| US20140273152A1 (en) * | 2013-03-13 | 2014-09-18 | Oregon Health & Science University | Recombinant manganese oxidase |
| FI3585910T3 (fi) | 2017-02-24 | 2024-06-19 | Danisco Us Inc | Koostumukset ja menetelmät proteiinin tuotannon lisäämiseksi bacillus licheniformiksessa |
| DK3607097T3 (da) | 2017-04-07 | 2023-09-18 | Dupont Nutrition Biosci Aps | Bacillus-værtsceller, der danner beta-galactosidaser og lactaser i fravær af p-nitrobenzylesterase-sideaktivitet |
| WO2019040412A1 (en) | 2017-08-23 | 2019-02-28 | Danisco Us Inc | METHODS AND COMPOSITIONS FOR EFFICIENT GENETIC MODIFICATION OF BACILLUS LICHENIFORMIS STRAINS |
| JP7218985B2 (ja) | 2017-09-13 | 2023-02-07 | ダニスコ・ユーエス・インク | バチルス属（Ｂａｃｉｌｌｕｓ）におけるタンパク質産生の増加のための改変５’－非翻訳領域（ＵＴＲ）配列 |
| EP3735478B1 (de) | 2018-01-03 | 2023-08-16 | Danisco US Inc. | Mutant und genetisch modifizierte bacillus-zellen und verfahren dafür für gesteigerte proteinproduktion |
| KR102755260B1 (ko) * | 2018-07-25 | 2025-01-14 | 플래그쉽 파이어니어링 이노베이션스 브이, 인크. | 농업용 조성물 및 관련 방법 |
| KR20210096629A (ko) | 2018-11-28 | 2021-08-05 | 다니스코 유에스 인크. | 바실러스 세포에서의 향상된 단백질 생산을 위한 신규 프로모터 서열 및 이의 방법 |
| CN110144626A (zh) * | 2019-03-29 | 2019-08-20 | 浙江工业大学 | 一种启动子文库的构建方法 |
| CA3136113A1 (en) | 2019-04-05 | 2020-10-08 | Danisco Us Inc. | Methods for polynucleotide integration into the genome of bacillus using dual circular recombinant dna constructs and compositions thereof |
| CA3136114A1 (en) | 2019-04-05 | 2020-10-08 | Danisco Us Inc. | Methods for integrating a donor dna sequence into the genome of bacillus using linear recombinant dna constructs and compositions thereof |
| KR102194368B1 (ko) * | 2019-06-05 | 2020-12-24 | 서울시립대학교 산학협력단 | 미생물의 유전체를 개량하기 위한 변이 균주, 이의 제조방법 및 이를 이용한 미생물의 유전체 개량 방법 |
| EP4031560A1 (de) | 2019-08-14 | 2022-07-27 | Danisco US Inc | Zusammensetzungen und verfahren zur erhöhung der proteinproduktion in bacillus licheniformis |
| WO2021086606A1 (en) | 2019-10-28 | 2021-05-06 | Danisco Us Inc | Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases |
| JP7656603B2 (ja) | 2019-11-11 | 2025-04-03 | ダニスコ・ユーエス・インク | バチルス属（ｂａｃｉｌｌｕｓ）細胞における強化されたタンパク質産生のための組成物及び方法 |
| KR20220098245A (ko) | 2019-11-19 | 2022-07-11 | 다니스코 유에스 인크. | 바실러스 게놈 및 이의 조성물을 변형시키기 위한 선택 마커-부재 방법 |
| EP4090738A1 (de) | 2020-01-15 | 2022-11-23 | Danisco US Inc. | Zusammensetzungen und verfahren zur verbesserten proteinproduktion in bacillus licheniformis |
| EP4294823A1 (de) | 2021-02-22 | 2023-12-27 | Danisco US Inc. | Verfahren und zusammensetzungen zur herstellung von proteinen von interesse in pigmentdefizienten bacillus-zellen |
| US20240263185A1 (en) | 2021-05-24 | 2024-08-08 | Danisco Us Inc. | Compositions and methods for enhanced protein production in bacillus cells |
| CN118556120A (zh) | 2021-11-16 | 2024-08-27 | 丹尼斯科美国公司 | 用于增强芽孢杆菌属细胞中蛋白质产生的组合物和方法 |
| CN119278264A (zh) * | 2022-05-31 | 2025-01-07 | 松下知识产权经营株式会社 | 改造启动子、表达载体、微生物、物质的生产方法、改造蓝细菌和改造启动子的制作方法 |
| WO2025034713A2 (en) | 2023-08-09 | 2025-02-13 | Danisco Us Inc. | Compositions and methods for enhanced protein production in gram‑positive bacterial cells |
| WO2026090396A1 (en) | 2024-10-24 | 2026-04-30 | Danisco Us Inc. | Compositions and methods for enhanced protein production in gram‑positive bacterial cells |
| WO2026136410A1 (en) | 2024-12-17 | 2026-06-25 | Danisco Us Inc. | Intracellular co-expression of protease inhibitor in bacillus improves cell viability and enhances protease production |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5532142A (en) * | 1993-02-12 | 1996-07-02 | Board Of Regents, The University Of Texas System | Method of isolation and purification of fusion polypeptides |
| WO1994025609A1 (en) * | 1993-04-28 | 1994-11-10 | Hybritech Incorporated | Method for creating optimized regulatory regions affecting protein expression and protein trafficking |
| US6140087A (en) * | 1993-06-24 | 2000-10-31 | Advec, Inc. | Adenovirus vectors for gene therapy |
| EP0937098B1 (de) * | 1995-06-07 | 2002-08-14 | Invitrogen Corporation | Rekombinatorische klonierung in vitro unter verwendung genmanipulierter rekombinationsorte |
| DE69739121D1 (de) * | 1996-08-23 | 2009-01-02 | Peter Ruhdal Jensen | Künstliche promotorbibliotheken für ausgewählte organismen sowie daraus entstammende promotoren |
| US5851808A (en) * | 1997-02-28 | 1998-12-22 | Baylor College Of Medicine | Rapid subcloning using site-specific recombination |
| US6294358B1 (en) * | 1999-09-07 | 2001-09-25 | Thermogen, Inc. | Thermus promoters for gene expression |
-
2003
- 2003-04-18 JP JP2003586317A patent/JP2006513692A/ja not_active Withdrawn
- 2003-04-18 CA CA2830864A patent/CA2830864C/en not_active Expired - Fee Related
- 2003-04-18 CA CA2483006A patent/CA2483006C/en not_active Expired - Fee Related
- 2003-04-18 WO PCT/US2003/012044 patent/WO2003089604A2/en not_active Ceased
- 2003-04-18 AU AU2003230981A patent/AU2003230981A1/en not_active Abandoned
- 2003-04-18 US US10/505,844 patent/US20060057633A1/en not_active Abandoned
- 2003-04-18 EP EP03724099A patent/EP1576094B1/de not_active Expired - Lifetime
- 2003-04-18 AT AT03724099T patent/ATE526419T1/de not_active IP Right Cessation
- 2003-04-18 DK DK03724099.1T patent/DK1576094T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| WO2003089604A3 (en) | 2006-02-16 |
| DK1576094T3 (da) | 2012-01-02 |
| EP1576094B1 (de) | 2011-09-28 |
| EP1576094A2 (de) | 2005-09-21 |
| CA2830864C (en) | 2017-03-21 |
| US20060057633A1 (en) | 2006-03-16 |
| EP1576094A4 (de) | 2006-08-30 |
| CA2483006A1 (en) | 2003-10-30 |
| JP2006513692A (ja) | 2006-04-27 |
| CA2830864A1 (en) | 2003-10-30 |
| WO2003089604A2 (en) | 2003-10-30 |
| AU2003230981A1 (en) | 2003-11-03 |
| AU2003230981A8 (en) | 2003-11-03 |
| CA2483006C (en) | 2013-10-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK1576094T3 (da) | Fremgangsmåder til at danne modificerede promotere resulterende i varierende genekspressionsniveauer | |
| ATE401422T1 (de) | Verfahren zur erzeugung einer bibliothek aus bakterienklonen mit unterschiedlichen genexpressionsniveaus | |
| DE50001708D1 (de) | Verfahren zur mikrobiellen herstellung von l-valin | |
| ATE465241T1 (de) | Verfahren zur fermentativen herstellung von proteinen | |
| ATE440811T1 (de) | Verfahren zur umwandlung von synthesegas in c2+- alkohole enthaltende oxygenate | |
| DE502004005166D1 (de) | Expressionsvektor zur antibiotikafreien expression | |
| CN104347080A (zh) | 语音分析方法和装置、语音合成方法和装置、以及存储语音分析程序的介质 | |
| ATE340857T1 (de) | Verfahren zur herstellung von l-aminosäuren durch erhöhung des zellulären nadph | |
| ATE554483T1 (de) | Verfahren zur wiedergabe textbasierter untertiteldaten von einem datenträger unter verwendung von wählbaren stilinformationen | |
| KR102012430B1 (ko) | 미생물 증식을 이용한 수질 관리 장치 및 그 방법 | |
| ATE330014T1 (de) | Verfahren zur herstellung von 1,3-propanediol durch einen rekombinanten micro-organismus in abwesenheit von coenzym b12 | |
| ATE326523T1 (de) | Verfahren zur fermentativen herstellung von l- methionin | |
| ATE524563T1 (de) | System zur herstellung synthetischer promotoren | |
| BR0309766A (pt) | Promotores usp semente especìficos para expressar genes em vegetais | |
| GB2450843A (en) | Mutant HSV, materials and methods for generation of mutant HSV | |
| CA2600241A1 (en) | Voice-scrambling-signal creation method and apparatus, and computer-readable storage medium therefor | |
| ATE529507T1 (de) | Verfahren zur produktion von erythro- oder threo- 2-amino-3-hydroxypropionsäureester, neuartige carbonylreduktase, gen für die reduktase, vektor, transformante und verfahren zur produktion von optisch aktivem alkohol unter verwendung dieser | |
| ATE423199T1 (de) | Verfahren zur herstelllung von menschlichem thrombin mittels genmodifikationstechnik | |
| UA91686C2 (en) | Biochemical synhesis of 1.4-butandiamine | |
| ATE305973T1 (de) | Verfahren zur herstellung von d-pantothensäure und/oder deren salzen | |
| EP1519382A3 (de) | Informationswiedergabeverfahren, Aufzeichnungsmedium mit Informationswiedergabeprogramm und Informationswiedergabevorrichtung | |
| Chen et al. | De novo transcriptome assembly of RNA-Seq reads with different strategies | |
| GB2450059A (en) | Culture system and method for propagation of human blastocyst-derived stem cells | |
| SG125950A1 (en) | Demand dispatch system and method | |
| TWI264708B (en) | Method and device for recording text data, and music disk, computer program, and regeneration device for music disk |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |