ATE526419T1 - Verfahren zur erzeugung zu variierenden genexpressionsniveaus führender modifizierter promotoren - Google Patents
Verfahren zur erzeugung zu variierenden genexpressionsniveaus führender modifizierter promotorenInfo
- Publication number
- ATE526419T1 ATE526419T1 AT03724099T AT03724099T ATE526419T1 AT E526419 T1 ATE526419 T1 AT E526419T1 AT 03724099 T AT03724099 T AT 03724099T AT 03724099 T AT03724099 T AT 03724099T AT E526419 T1 ATE526419 T1 AT E526419T1
- Authority
- AT
- Austria
- Prior art keywords
- expression levels
- gene expression
- promotors
- leading
- modified
- Prior art date
Links
- 230000014509 gene expression Effects 0.000 title abstract 3
- 230000001580 bacterial effect Effects 0.000 abstract 3
- 239000002243 precursor Substances 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B30/00—Methods of screening libraries
- C40B30/06—Methods of screening libraries by measuring effects on living organisms, tissues or cells
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Virology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US37462702P | 2002-04-22 | 2002-04-22 | |
| US37473502P | 2002-04-22 | 2002-04-22 | |
| PCT/US2003/012044 WO2003089604A2 (en) | 2002-04-22 | 2003-04-18 | Methods of creating modified promoters resulting in varying levels of gene expression |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE526419T1 true ATE526419T1 (de) | 2011-10-15 |
Family
ID=29254561
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03724099T ATE526419T1 (de) | 2002-04-22 | 2003-04-18 | Verfahren zur erzeugung zu variierenden genexpressionsniveaus führender modifizierter promotoren |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20060057633A1 (de) |
| EP (1) | EP1576094B1 (de) |
| JP (1) | JP2006513692A (de) |
| AT (1) | ATE526419T1 (de) |
| AU (1) | AU2003230981A1 (de) |
| CA (2) | CA2483006C (de) |
| DK (1) | DK1576094T3 (de) |
| WO (1) | WO2003089604A2 (de) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060121581A1 (en) | 2002-10-04 | 2006-06-08 | Cervin Marguerite A | Production of bacterial strains cross reference to related applications |
| KR101130587B1 (ko) * | 2002-10-04 | 2012-03-30 | 다니스코 유에스 인크. | 생물질 제조용 글루코오스 수송 돌연변이 |
| US20050079617A1 (en) | 2003-10-03 | 2005-04-14 | Cervin Marguerite A. | Glucose transport mutants for production of biomaterial |
| WO2011053957A2 (en) * | 2009-11-02 | 2011-05-05 | Gen9, Inc. | Compositions and methods for the regulation of multiple genes of interest in a cell |
| US20140273152A1 (en) * | 2013-03-13 | 2014-09-18 | Oregon Health & Science University | Recombinant manganese oxidase |
| JP7231228B2 (ja) | 2017-02-24 | 2023-03-01 | ダニスコ・ユーエス・インク | バチルス・リケニフォルミスにおける増加したタンパク質産生のための組成物及び方法 |
| DK3607097T3 (da) | 2017-04-07 | 2023-09-18 | Dupont Nutrition Biosci Aps | Bacillus-værtsceller, der danner beta-galactosidaser og lactaser i fravær af p-nitrobenzylesterase-sideaktivitet |
| WO2019040412A1 (en) | 2017-08-23 | 2019-02-28 | Danisco Us Inc | METHODS AND COMPOSITIONS FOR EFFICIENT GENETIC MODIFICATION OF BACILLUS LICHENIFORMIS STRAINS |
| KR20200047668A (ko) | 2017-09-13 | 2020-05-07 | 다니스코 유에스 인크. | 바실러스에서 증가된 단백질 생산을 위한 변형된 5'-비번역 영역(utr) 서열 |
| KR102715197B1 (ko) | 2018-01-03 | 2024-10-08 | 다니스코 유에스 인크. | 증가된 단백질 생산을 위한 돌연변이체 및 유전자 변형된 바실러스 세포 및 이의 방법 |
| AR117623A1 (es) * | 2018-07-25 | 2021-08-18 | Flagship Pioneering Innovations V Inc | Composiciones y métodos relacionados para la agricultura |
| WO2020112609A1 (en) | 2018-11-28 | 2020-06-04 | Danisco Us Inc | Novel promoter sequences and methods thereof for enhanced protein production in bacillus cells |
| CN110144626A (zh) * | 2019-03-29 | 2019-08-20 | 浙江工业大学 | 一种启动子文库的构建方法 |
| EP3947656A1 (de) | 2019-04-05 | 2022-02-09 | Danisco US Inc. | Verfahren zur integration von polynukleotiden in das genom von bacillus unter verwendung von zwei zirkulären rekombinanten dna-konstrukten und zusammensetzungen davon |
| KR102931179B1 (ko) | 2019-04-05 | 2026-02-25 | 다니스코 유에스 인크. | 선형 재조합 dna 작제물 및 이의 조성물을 이용하여 공여 dna 서열을 바실러스 게놈 내에 통합시키기 위한 방법 |
| KR102194368B1 (ko) * | 2019-06-05 | 2020-12-24 | 서울시립대학교 산학협력단 | 미생물의 유전체를 개량하기 위한 변이 균주, 이의 제조방법 및 이를 이용한 미생물의 유전체 개량 방법 |
| EP4031560A1 (de) | 2019-08-14 | 2022-07-27 | Danisco US Inc | Zusammensetzungen und verfahren zur erhöhung der proteinproduktion in bacillus licheniformis |
| WO2021086606A1 (en) | 2019-10-28 | 2021-05-06 | Danisco Us Inc | Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases |
| WO2021096857A1 (en) | 2019-11-11 | 2021-05-20 | Danisco Us Inc | Compositions and methods for enhanced protein production in bacillus cells |
| US12612649B2 (en) | 2019-11-19 | 2026-04-28 | Danisco Us Inc. | Selection marker free methods for modifying the genome of bacillus and compositions thereof |
| CN114945665A (zh) | 2020-01-15 | 2022-08-26 | 丹尼斯科美国公司 | 用于增强地衣芽孢杆菌中蛋白产生的组合物和方法 |
| WO2022178432A1 (en) | 2021-02-22 | 2022-08-25 | Danisco Us Inc. | Methods and compositions for producing proteins of interest in pigment deficient bacillus cells |
| US20240263185A1 (en) | 2021-05-24 | 2024-08-08 | Danisco Us Inc. | Compositions and methods for enhanced protein production in bacillus cells |
| KR20240099301A (ko) | 2021-11-16 | 2024-06-28 | 다니스코 유에스 인크. | 바실러스 세포에서 단백질 생산을 향상시키기 위한 조성물 및 방법 |
| CN119278264A (zh) * | 2022-05-31 | 2025-01-07 | 松下知识产权经营株式会社 | 改造启动子、表达载体、微生物、物质的生产方法、改造蓝细菌和改造启动子的制作方法 |
| WO2025034713A2 (en) | 2023-08-09 | 2025-02-13 | Danisco Us Inc. | Compositions and methods for enhanced protein production in gram‑positive bacterial cells |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5532142A (en) * | 1993-02-12 | 1996-07-02 | Board Of Regents, The University Of Texas System | Method of isolation and purification of fusion polypeptides |
| AU6776194A (en) * | 1993-04-28 | 1994-11-21 | Hybritech Incorporated | Method for creating optimized regulatory regions affecting protein expression and protein trafficking |
| US6140087A (en) * | 1993-06-24 | 2000-10-31 | Advec, Inc. | Adenovirus vectors for gene therapy |
| EP2322614A1 (de) * | 1995-06-07 | 2011-05-18 | Life Technologies Corporation | Rekombinatorische Klonierung unter Verwendung genmanipulierter Rekombinationsorte |
| EP2034021A1 (de) * | 1996-08-23 | 2009-03-11 | Peter Ruhdal Jensen | Künstliche promotorbibliotheken für ausgewählte organismen sowie daraus entstammende promotoren |
| US5851808A (en) * | 1997-02-28 | 1998-12-22 | Baylor College Of Medicine | Rapid subcloning using site-specific recombination |
| US6294358B1 (en) * | 1999-09-07 | 2001-09-25 | Thermogen, Inc. | Thermus promoters for gene expression |
-
2003
- 2003-04-18 JP JP2003586317A patent/JP2006513692A/ja not_active Withdrawn
- 2003-04-18 CA CA2483006A patent/CA2483006C/en not_active Expired - Fee Related
- 2003-04-18 AT AT03724099T patent/ATE526419T1/de not_active IP Right Cessation
- 2003-04-18 US US10/505,844 patent/US20060057633A1/en not_active Abandoned
- 2003-04-18 CA CA2830864A patent/CA2830864C/en not_active Expired - Fee Related
- 2003-04-18 EP EP03724099A patent/EP1576094B1/de not_active Expired - Lifetime
- 2003-04-18 AU AU2003230981A patent/AU2003230981A1/en not_active Abandoned
- 2003-04-18 WO PCT/US2003/012044 patent/WO2003089604A2/en not_active Ceased
- 2003-04-18 DK DK03724099.1T patent/DK1576094T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| EP1576094A4 (de) | 2006-08-30 |
| US20060057633A1 (en) | 2006-03-16 |
| CA2830864A1 (en) | 2003-10-30 |
| WO2003089604A3 (en) | 2006-02-16 |
| CA2483006C (en) | 2013-10-29 |
| JP2006513692A (ja) | 2006-04-27 |
| DK1576094T3 (da) | 2012-01-02 |
| EP1576094B1 (de) | 2011-09-28 |
| AU2003230981A1 (en) | 2003-11-03 |
| AU2003230981A8 (en) | 2003-11-03 |
| CA2483006A1 (en) | 2003-10-30 |
| EP1576094A2 (de) | 2005-09-21 |
| WO2003089604A2 (en) | 2003-10-30 |
| CA2830864C (en) | 2017-03-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |