ATE526419T1 - Verfahren zur erzeugung zu variierenden genexpressionsniveaus führender modifizierter promotoren - Google Patents

Verfahren zur erzeugung zu variierenden genexpressionsniveaus führender modifizierter promotoren

Info

Publication number
ATE526419T1
ATE526419T1 AT03724099T AT03724099T ATE526419T1 AT E526419 T1 ATE526419 T1 AT E526419T1 AT 03724099 T AT03724099 T AT 03724099T AT 03724099 T AT03724099 T AT 03724099T AT E526419 T1 ATE526419 T1 AT E526419T1
Authority
AT
Austria
Prior art keywords
expression levels
gene expression
promotors
leading
modified
Prior art date
Application number
AT03724099T
Other languages
English (en)
Inventor
Marguerite Cervin
Fernando Valle
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=29254561&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE526419(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Application granted granted Critical
Publication of ATE526419T1 publication Critical patent/ATE526419T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/06—Methods of screening libraries by measuring effects on living organisms, tissues or cells
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT03724099T 2002-04-22 2003-04-18 Verfahren zur erzeugung zu variierenden genexpressionsniveaus führender modifizierter promotoren ATE526419T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US37473502P 2002-04-22 2002-04-22
US37462702P 2002-04-22 2002-04-22
PCT/US2003/012044 WO2003089604A2 (en) 2002-04-22 2003-04-18 Methods of creating modified promoters resulting in varying levels of gene expression

Publications (1)

Publication Number Publication Date
ATE526419T1 true ATE526419T1 (de) 2011-10-15

Family

ID=29254561

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03724099T ATE526419T1 (de) 2002-04-22 2003-04-18 Verfahren zur erzeugung zu variierenden genexpressionsniveaus führender modifizierter promotoren

Country Status (8)

Country Link
US (1) US20060057633A1 (de)
EP (1) EP1576094B1 (de)
JP (1) JP2006513692A (de)
AT (1) ATE526419T1 (de)
AU (1) AU2003230981A1 (de)
CA (2) CA2483006C (de)
DK (1) DK1576094T3 (de)
WO (1) WO2003089604A2 (de)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1546304B1 (de) 2002-10-04 2013-05-08 Danisco US Inc. VERBESSERTE PRODUKTION VON BAKTERIENSTûMMEN
US20050079617A1 (en) 2003-10-03 2005-04-14 Cervin Marguerite A. Glucose transport mutants for production of biomaterial
JP5041570B2 (ja) * 2002-10-04 2012-10-03 ジェネンコー・インターナショナル・インク 生物資源の生産に対するグルコース輸送変異体
WO2011053957A2 (en) * 2009-11-02 2011-05-05 Gen9, Inc. Compositions and methods for the regulation of multiple genes of interest in a cell
US20140273152A1 (en) * 2013-03-13 2014-09-18 Oregon Health & Science University Recombinant manganese oxidase
DK3585910T3 (da) 2017-02-24 2024-06-17 Danisco Us Inc Sammensætninger og fremgangsmåder til forøget proteinproduktion i bacillus licheniformis
WO2018187524A1 (en) 2017-04-07 2018-10-11 Dupont Nutrition Biosciences Aps BACILLUS HOST CELLS PRODUCING β-GALACTOSIDASES AND LACTASES IN THE ABSENCE OF P-NITROBENZYLESTERASE SIDE ACTIVITY
WO2019040412A1 (en) 2017-08-23 2019-02-28 Danisco Us Inc METHODS AND COMPOSITIONS FOR EFFICIENT GENETIC MODIFICATION OF BACILLUS LICHENIFORMIS STRAINS
WO2019055261A1 (en) 2017-09-13 2019-03-21 Danisco Us Inc MODIFIED 5 'NON-TRANSLATED REGION (UTR) SEQUENCES FOR INCREASED PROTEIN PRODUCTION IN BACILLUS
CN111670244B (zh) 2018-01-03 2023-08-01 丹尼斯科美国公司 突变的和遗传修饰的芽孢杆菌属细胞及其用于增加蛋白质产生的方法
MA53391A (fr) * 2018-07-25 2021-06-02 Flagship Pioneering Innovations V Inc Compositions pour l'agriculture et procédés associés
JP2022509215A (ja) 2018-11-28 2022-01-20 ダニスコ・ユーエス・インク バチルス属（ｂａｃｉｌｌｕｓ）細胞におけるタンパク質産生増強のための新規なプロモーター配列及びその方法
CN110144626A (zh) * 2019-03-29 2019-08-20 浙江工业大学 一种启动子文库的构建方法
US20220177923A1 (en) 2019-04-05 2022-06-09 Danisco Us Inc Methods for integrating a donor DNA sequence into the genome of bacillus using linear recombinant DNA constructs and compositions thereof
JP2022526414A (ja) 2019-04-05 2022-05-24 ダニスコ・ユーエス・インク 二重環状組換えＤＮＡコンストラクトを使用するバチルス（Ｂａｃｉｌｌｕｓ）のゲノムへのポリヌクレオチド組込みのための方法及びその組成物
KR102194368B1 (ko) * 2019-06-05 2020-12-24 서울시립대학교 산학협력단 미생물의 유전체를 개량하기 위한 변이 균주, 이의 제조방법 및 이를 이용한 미생물의 유전체 개량 방법
WO2021030267A1 (en) 2019-08-14 2021-02-18 Danisco Us Inc Compositions and methods for increased protein production in bacillus licheniformis
WO2021086606A1 (en) 2019-10-28 2021-05-06 Danisco Us Inc Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases
JP7656603B2 (ja) 2019-11-11 2025-04-03 ダニスコ・ユーエス・インク バチルス属（ｂａｃｉｌｌｕｓ）細胞における強化されたタンパク質産生のための組成物及び方法
EP4061939A1 (de) 2019-11-19 2022-09-28 Danisco US Inc. Selektionsmarkerfreies verfahren zur modifizierung des genoms von bacillus und zusammensetzungen davon
WO2021146411A1 (en) 2020-01-15 2021-07-22 Danisco Us Inc Compositions and methods for enhanced protein production in bacillus licheniformis
US20240101611A1 (en) 2021-02-22 2024-03-28 Danisco Us Inc. Methods and compositions for producing proteins of interest in pigment deficient bacillus cells
US20240263185A1 (en) 2021-05-24 2024-08-08 Danisco Us Inc. Compositions and methods for enhanced protein production in bacillus cells
CN118556120A (zh) 2021-11-16 2024-08-27 丹尼斯科美国公司 用于增强芽孢杆菌属细胞中蛋白质产生的组合物和方法
JPWO2023233996A1 (de) * 2022-05-31 2023-12-07
WO2025034713A2 (en) 2023-08-09 2025-02-13 Danisco Us Inc. Compositions and methods for enhanced protein production in gram‑positive bacterial cells

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5532142A (en) * 1993-02-12 1996-07-02 Board Of Regents, The University Of Texas System Method of isolation and purification of fusion polypeptides
AU6776194A (en) * 1993-04-28 1994-11-21 Hybritech Incorporated Method for creating optimized regulatory regions affecting protein expression and protein trafficking
US6140087A (en) * 1993-06-24 2000-10-31 Advec, Inc. Adenovirus vectors for gene therapy
CA2226463A1 (en) * 1995-06-07 1996-12-19 Life Technologies Corporation Recombinational cloning using engineered recombination sites
JP2001503249A (ja) * 1996-08-23 2001-03-13 ルーダル イェンセン，ペテル 選択された生物のための人工プロモーターライブラリー及び該ライブラリー由来のプロモーター
US5851808A (en) * 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
US6294358B1 (en) * 1999-09-07 2001-09-25 Thermogen, Inc. Thermus promoters for gene expression

Also Published As

Publication number Publication date
US20060057633A1 (en) 2006-03-16
AU2003230981A1 (en) 2003-11-03
DK1576094T3 (da) 2012-01-02
EP1576094A4 (de) 2006-08-30
WO2003089604A2 (en) 2003-10-30
CA2830864A1 (en) 2003-10-30
CA2830864C (en) 2017-03-21
EP1576094A2 (de) 2005-09-21
JP2006513692A (ja) 2006-04-27
CA2483006A1 (en) 2003-10-30
EP1576094B1 (de) 2011-09-28
CA2483006C (en) 2013-10-29
WO2003089604A3 (en) 2006-02-16
AU2003230981A8 (en) 2003-11-03

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