DK1670315T3 - Fremgangsmåder til anvendelse af fedtafledte regenerative celler i behandlingen af perifer karsygdom - Google Patents
Fremgangsmåder til anvendelse af fedtafledte regenerative celler i behandlingen af perifer karsygdom Download PDFInfo
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- FREMGANGSMÅDER TIL ANVENDELSE AF FEDTAFLEDTE REGENERATIVE CELLER I BEHANDLINGEN AF PERIFER KARSYGDOM1. Sammensætning omfattende en koncentreret population af fedtvævsafledte celler til anvendelse i behandlingen af perifer karsygdom (PVD) uden for hjertet og centralnervesystemet hos et subjekt med behov derfor, hvori den koncentrerede population af fedtafledte celler skal administreres til en patient med behov derfor uden at dyrke de fedtafledte celler, før de administreres til subjektet, hvori de fedtafledte celler opnås ved at behandle fedtvæv, så de fedtafledte celler separeres fra modne adipocytter og bindevæv, og vaske, separere og koncentrere de fedtafledte celler, hvori den koncentrerede population af fedtvævsafledte celler omfatter en minimumscellekoncentration på omkring 1 x 105 til omkring 1 x 107 celler/ml, og hvori mindst 0,1 % af cellekomponenten af den koncentrerede population af fedtafledte celler er stamceller.
- 2. Sammensætning til anvendelse ifølge krav 1, hvori den koncentrerede population af fedtvævsafledte celler endvidere omfatter fedtafledte progenitor-celler.
- 3. Sammensætning til anvendelse ifølge et hvilket som helst af de foregående krav, hvori sammensætningen endvidere omfatter et additiv.
- 4. Sammensætning til anvendelse ifølge et hvilket som helst af de foregående krav, hvori sammensætningen omfatter et scaffold.
- 5. Sammensætning til anvendelse ifølge krav 4, hvori scaffoldet er et resorberbart scaffold.
- 6. Sammensætning til anvendelse ifølge krav 4, hvori cellerne administreres i kombination med væv eller vævsfragmenter, og hvori ekstraktion og koncentration af fedtvævet og placering af cellerne sker i en enkelt operativ procedures kontekst.
- 7. Sammensætning til anvendelse ifølge et hvilket som helst af de foregående krav, hvori de fedtvævsafledte celler er modificeret ved genoverførsel.
- 8. Sammensætning til anvendelse ifølge et hvilket som helst af de foregående krav, hvori den perifere karsygdom er karakteriseret ved en aberrant blodgennemstrømning, arteriosklerose en traumatisk skade på et blodkar eller en inflammatorisk arteritis.
- 9. Sammensætning til anvendelse ifølge et hvilket som helst af de foregående krav, hvori den perifere karsygdom stammer fra kulde, stress eller rygning.
- 10. Sammensætning til anvendelse ifølge et hvilket som helst af de foregående krav, hvori sammensætningen endvidere omfatter en angiogen faktor eller et immunsupprimerende lægemiddel.
- 11. Sammensætning ifølge et hvilket som helst af de foregående krav, hvori sammensætningen er formuleret til intravenøs, intramuskulær eller subkutan administration.
- 12. Sammensætning ifølge et hvilket som helst af de foregående krav, hvori behandlingen af perifer karsygdom (PVD) uden for hjertet og centralnervesystemet omfatter reduktion i claudicatio.
- 13. Sammensætning ifølge et hvilket som helst af de foregående krav, hvori behandlingen af perifer karsygdom (PVD) uden for hjertet og centralnervesystemet omfatter heling af iskæmiske ulcerationer.
- 14. Sammensætning ifølge et hvilket som helst af de foregående krav, hvori behandlingen af perifer karsygdom (PVD) uden for hjertet og centralnervesystemet omfatter øgning af oxygenkoncentrationen i vævet målt ved kropspletysmografi.
- 15. Sammensætning ifølge et hvilket som helst af de foregående krav, hvori behandlingen af perifer karsygdom (PVD) uden for hjertet og centralnervesystemet omfatter forbedring i det ankel-brakiale indeks (ABI).
- 16. Sammensætning ifølge et hvilket som helst af de foregående krav, hvori sammensætningen er formuleret til flerdosisadministration.
- 17. Sammensætning til anvendelse ifølge krav 3, hvori additivet omfatter andre celler, væv eller vævsfragmenter.
- 18. Anvendelse af en sammensætning omfattende en koncentreret population af fedtvævsafledte celler til fremstilling af et medikament til behandling af perifer karsygdom (PVD) uden for hjertet og centralnervesystemet hos et subjekt med behov derfor, hvori den koncentrerede population af fedtafledte celler skal administreres til en patient med behov derfor uden at dyrke de fedtafledte celler, før de administreres til subjektet, hvori de fedtafledte celler opnås ved at behandle fedtvæv, så de fedtafledte celler separeres fra modne adipocytter og bindevæv, og vaske, separere og koncentrere de fedtafledte celler, hvori den koncentrerede population af fedtvævsafledte celler omfatter en minimumscellekoncentration på omkring 1 x 105 til omkring 1 x 107 celler/ml, og hvori mindst 0.1 % af cellekomponenten af den koncentrerede population af fedtafledte celler er stamceller.
- 19. Anvendelse af en sammensætning ifølge krav 18, hvori den koncentrerede population af fedtvævsafledte celler endvidere omfatter fedtafledte progenitor-celler.
- 20. Anvendelse af en sammensætning ifølge krav 18 eller krav 19, hvori sammensætningen endvidere omfatter et additiv.
- 21. Anvendelse af en sammensætning ifølge et hvilket som helst af kravene 18, 19 og 20, hvori sammensætningen omfatter et scaffold.
- 22. Anvendelse af en sammensætning ifølge krav 21, hvori scaffoldet er et resorberbart scaffold.
- 23. Anvendelse af en sammensætning ifølge krav 21, hvori cellerne administreres i kombination med væv eller vævsfragmenter, og hvori ekstraktion og koncentration af fedtvævet og placering af cellerne sker i en enkelt operativ procedures kontekst.
- 24. Anvendelse af en sammensætning ifølge et hvilket som helst af kravene 18-23, hvori de fedtvævsafledte celler er modificeret ved genoverførsel.
- 25. Anvendelse af en sammensætning til anvendelse ifølge et hvilket som helst af kravene 18-24, hvori den perifere karsygdom er karakteriseret ved en utilstrækkelig blodgennemstrømning, arteriosklerose en traumatisk skade på et blodkar eller en inflammatorisk arteritis.
- 26. Anvendelse af en sammensætning ifølge et hvilket som helst af kravene 18-25, hvori den perifere karsygdom stammer fra kulde, stress eller rygning.
- 27. Anvendelse af en sammensætning ifølge et hvilket som helst af kravene 18-26, hvori sammensætningen endvidere omfatter en angiogen faktor eller et immunsupprimerende lægemiddel.
- 28. Anvendelse af en sammensætning ifølge et hvilket som helst af kravene 18-27, hvori sammensætningen er formuleret til intravenøs, intramuskulær eller subkutan administration.
- 29. Anvendelse af en sammensætning ifølge et hvilket som helst af kravene 18-28, hvori behandlingen af perifer karsygdom (PVD) uden for hjertet og centralnervesystemet omfatter reduktion i claudicatio.
- 30. Anvendelse af en sammensætning ifølge et hvilket som helst af kravene 18-29, hvori behandlingen af perifer karsygdom (PVD) uden for hjertet og centralnervesystemet omfatter heling af iskæmiske ulcerationer.
- 31. Anvendelse af en sammensætning ifølge et hvilket som helst af kravene 18-30, hvori behandlingen af perifer karsygdom (PVD) uden for hjertet og centralnervesystemet omfatter øgning af oxygenkoncentrationen i vævet målt ved kropspletysmografi.
- 32. Anvendelse af en sammensætning ifølge et hvilket som helst af kraven 18-31, hvori behandlingen af perifer karsygdom (PVD) uden for hjertet og centralnervesystemet omfatter forbedring i det ankel-brakiale indeks (ABI).
- 33. Anvendelse af en sammensætning ifølge et hvilket som helst af kravene 18-32, hvori sammensætningen er formuleret til flerdosisadministration.
- 34. Anvendelse af en sammensætning til anvendelse ifølge krav 20, hvori additivet omfatter andre celler, væv eller vævsfragmenter.
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| DK04756641.9T DK1670315T3 (da) | 2003-09-17 | 2004-07-01 | Fremgangsmåder til anvendelse af fedtafledte regenerative celler i behandlingen af perifer karsygdom |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10589268B2 (en) | 2016-06-08 | 2020-03-17 | The Regents Of The University Of California | Method and device for processing tissues and cells |
| US10683480B2 (en) | 2013-06-21 | 2020-06-16 | The Regents Of The University Of California | Microfluidic tumor tissue dissociation device and method |
| US10722540B1 (en) | 2016-02-01 | 2020-07-28 | The Regents Of The University Of California | Microfluidic device and method for shear stress-induced transformation of cells |
Families Citing this family (78)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AUPR298901A0 (en) | 2001-02-07 | 2001-03-08 | McComb Foundation, Inc., The | Cell suspension preparation technique and device |
| US20050095228A1 (en) | 2001-12-07 | 2005-05-05 | Fraser John K. | Methods of using regenerative cells in the treatment of peripheral vascular disease and related disorders |
| US7595043B2 (en) | 2001-12-07 | 2009-09-29 | Cytori Therapeutics, Inc. | Method for processing and using adipose-derived stem cells |
| US7585670B2 (en) | 2001-12-07 | 2009-09-08 | Cytori Therapeutics, Inc. | Automated methods for isolating and using clinically safe adipose derived regenerative cells |
| US7771716B2 (en) | 2001-12-07 | 2010-08-10 | Cytori Therapeutics, Inc. | Methods of using regenerative cells in the treatment of musculoskeletal disorders |
| US9597395B2 (en) | 2001-12-07 | 2017-03-21 | Cytori Therapeutics, Inc. | Methods of using adipose tissue-derived cells in the treatment of cardiovascular conditions |
| US7514075B2 (en) | 2001-12-07 | 2009-04-07 | Cytori Therapeutics, Inc. | Systems and methods for separating and concentrating adipose derived stem cells from tissue |
| US20050048035A1 (en) | 2001-12-07 | 2005-03-03 | Fraser John K. | Methods of using regenerative cells in the treatment of stroke and related diseases and disorders |
| KR100930139B1 (ko) | 2001-12-07 | 2009-12-07 | 사이토리 테라퓨틱스, 인크. | 처리된 리포애스퍼레이트 세포로 환자를 치료하기 위한시스템 및 방법 |
| US20030205538A1 (en) | 2002-05-03 | 2003-11-06 | Randel Dorian | Methods and apparatus for isolating platelets from blood |
| US7832566B2 (en) | 2002-05-24 | 2010-11-16 | Biomet Biologics, Llc | Method and apparatus for separating and concentrating a component from a multi-component material including macroparticles |
| US7992725B2 (en) | 2002-05-03 | 2011-08-09 | Biomet Biologics, Llc | Buoy suspension fractionation system |
| WO2003099412A1 (en) | 2002-05-24 | 2003-12-04 | Biomet Manufacturing Corp. | Apparatus and method for separating and concentrating fluids containing multiple components |
| US20060278588A1 (en) | 2002-05-24 | 2006-12-14 | Woodell-May Jennifer E | Apparatus and method for separating and concentrating fluids containing multiple components |
| US7845499B2 (en) | 2002-05-24 | 2010-12-07 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
| JP4731556B2 (ja) * | 2004-07-02 | 2011-07-27 | サイトリ セラピューティクス インコーポレイテッド | 臨床的に安全な脂肪組織由来再生細胞を単離し、使用するためのシステムおよび方法 |
| ES2426941T3 (es) * | 2005-02-07 | 2013-10-25 | Hanuman Llc | Aparato y procedimiento de concentrados de plasma rico en plaquetas |
| US7708152B2 (en) | 2005-02-07 | 2010-05-04 | Hanuman Llc | Method and apparatus for preparing platelet rich plasma and concentrates thereof |
| US7866485B2 (en) | 2005-02-07 | 2011-01-11 | Hanuman, Llc | Apparatus and method for preparing platelet rich plasma and concentrates thereof |
| US8622997B2 (en) | 2005-03-23 | 2014-01-07 | Ronald D. Shippert | Tissue transfer method and apparatus |
| US7780649B2 (en) | 2005-03-23 | 2010-08-24 | Shippert Ronald D | Tissue transplantation method and apparatus |
| US9581942B1 (en) | 2005-03-23 | 2017-02-28 | Shippert Enterprises, Llc | Tissue transfer method and apparatus |
| US7794449B2 (en) | 2005-03-23 | 2010-09-14 | Shippert Ronald D | Tissue transplantation method and apparatus |
| US8062286B2 (en) | 2005-03-23 | 2011-11-22 | Shippert Ronald D | Tissue transplantation method and apparatus |
| US7789872B2 (en) | 2005-03-23 | 2010-09-07 | Shippert Ronald D | Tissue transplantation method and apparatus |
| DE602005026724D1 (de) * | 2005-05-25 | 2011-04-14 | Cytori Therapeutics Inc | Verfahren zur verwendung von aus fettgewebe stammenden zellen bei der behandlung von herz-kreislauf-leiden |
| AU2013216683B2 (en) * | 2005-05-25 | 2016-06-23 | Cytori Therapeutics, Inc. | Methods of using adipose tissue-derived cells in the treatment of cardiovascular conditions |
| US8567609B2 (en) | 2006-05-25 | 2013-10-29 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
| US8034014B2 (en) | 2007-03-06 | 2011-10-11 | Biomet Biologics, Llc | Angiogenesis initation and growth |
| US8328024B2 (en) | 2007-04-12 | 2012-12-11 | Hanuman, Llc | Buoy suspension fractionation system |
| JP5479319B2 (ja) | 2007-04-12 | 2014-04-23 | バイオメット・バイオロジックス・リミテッド・ライアビリティ・カンパニー | ブイ式懸濁液分画システム |
| KR20080103637A (ko) * | 2007-05-25 | 2008-11-28 | 주식회사 알앤엘바이오 | 지방유래 줄기세포를 함유하는 사지말단부 허혈성 질환의세포치료용 조성물 |
| JP5196890B2 (ja) * | 2007-06-29 | 2013-05-15 | オリンパス株式会社 | 有核細胞回収装置および有核細胞の回収方法 |
| MX2010003019A (es) | 2007-09-19 | 2010-04-30 | Pluristem Ltd | Celulas adherentes de los tejidos adiposo y de placenta y uso de las mismas en la terapia. |
| EP2567692B1 (en) | 2008-02-27 | 2016-04-06 | Biomet Biologics, LLC | Use of a device for obtaining interleukin-1 receptor antagonist rich solutions |
| WO2009111338A1 (en) | 2008-02-29 | 2009-09-11 | Biomet Manufacturing Corp. | A system and process for separating a material |
| US8012077B2 (en) | 2008-05-23 | 2011-09-06 | Biomet Biologics, Llc | Blood separating device |
| WO2010021993A1 (en) | 2008-08-19 | 2010-02-25 | Cytori Therapeutics, Inc. | Methods of using adipose tissue-derived cells in the treatment of the lymphatic system and malignant disease |
| US8187475B2 (en) | 2009-03-06 | 2012-05-29 | Biomet Biologics, Llc | Method and apparatus for producing autologous thrombin |
| US8313954B2 (en) | 2009-04-03 | 2012-11-20 | Biomet Biologics, Llc | All-in-one means of separating blood components |
| AU2010242780B2 (en) | 2009-05-01 | 2016-04-21 | Puregraft Llc | Systems, methods and compositions for optimizing tissue and cell enriched grafts |
| JP5431786B2 (ja) * | 2009-05-12 | 2014-03-05 | 國立中央大學 | 造血幹細胞を単離、生体外（ｅｘｖｉｖｏ）増殖および回収するためのシステムおよび方法 |
| US9011800B2 (en) | 2009-07-16 | 2015-04-21 | Biomet Biologics, Llc | Method and apparatus for separating biological materials |
| US8591391B2 (en) | 2010-04-12 | 2013-11-26 | Biomet Biologics, Llc | Method and apparatus for separating a material |
| US8887770B1 (en) | 2011-03-17 | 2014-11-18 | Ronald D. Shippert | Vessel fill control method and apparatus |
| JP5628131B2 (ja) * | 2011-10-19 | 2014-11-19 | サイトリ セラピューティクス インコーポレイテッド | 心血管状態の治療において脂肪組織由来細胞を使用する方法 |
| US9642956B2 (en) | 2012-08-27 | 2017-05-09 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
| US9468709B2 (en) | 2012-11-12 | 2016-10-18 | Shippert Enterprises, Llc | Syringe fill method and apparatus |
| AU2013205148B2 (en) | 2013-03-14 | 2014-10-30 | AVITA Medical Americas, LLC | Systems and methods for tissue processing and preparation of cell suspension therefrom |
| US10208095B2 (en) | 2013-03-15 | 2019-02-19 | Biomet Manufacturing, Llc | Methods for making cytokine compositions from tissues using non-centrifugal methods |
| US9895418B2 (en) | 2013-03-15 | 2018-02-20 | Biomet Biologics, Llc | Treatment of peripheral vascular disease using protein solutions |
| US9950035B2 (en) | 2013-03-15 | 2018-04-24 | Biomet Biologics, Llc | Methods and non-immunogenic compositions for treating inflammatory disorders |
| US20140271589A1 (en) | 2013-03-15 | 2014-09-18 | Biomet Biologics, Llc | Treatment of collagen defects using protein solutions |
| US10143725B2 (en) | 2013-03-15 | 2018-12-04 | Biomet Biologics, Llc | Treatment of pain using protein solutions |
| WO2015149098A1 (de) | 2014-03-31 | 2015-10-08 | Liporegena Gmbh | Vorrichtung zur entnahme und portionierung von fettgewebe |
| US10772997B2 (en) | 2014-05-15 | 2020-09-15 | Ronald D. Shippert | Tissue parcelization method and apparatus |
| US9713810B2 (en) | 2015-03-30 | 2017-07-25 | Biomet Biologics, Llc | Cell washing plunger using centrifugal force |
| US9757721B2 (en) | 2015-05-11 | 2017-09-12 | Biomet Biologics, Llc | Cell washing plunger using centrifugal force |
| EA037478B1 (ru) | 2015-08-04 | 2021-04-01 | Дьюк Юниверсити | Генетически кодируемые внутренне неупорядоченные полимеры-"невидимки" для доставки и способы их применения |
| US11752213B2 (en) | 2015-12-21 | 2023-09-12 | Duke University | Surfaces having reduced non-specific binding and antigenicity |
| WO2017141831A1 (ja) * | 2016-02-16 | 2017-08-24 | ロート製薬株式会社 | 脂肪組織由来間質細胞を含む虚血性疾患治療剤およびその製造方法 |
| WO2017210476A1 (en) | 2016-06-01 | 2017-12-07 | Duke University | Nonfouling biosensors |
| JP2020500150A (ja) | 2016-09-23 | 2020-01-09 | デューク ユニバーシティ | 下限臨界溶液温度挙動を有する非反復かつ非構造的ポリペプチド |
| WO2018132732A1 (en) | 2017-01-12 | 2018-07-19 | Duke University | Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature triggered hierarchical self-assembly |
| WO2018213320A1 (en) | 2017-05-15 | 2018-11-22 | Duke University | Recombinant production of hybrid lipid-biopolymer materials that self-assemble and encapsulate agents |
| WO2019006374A1 (en) | 2017-06-30 | 2019-01-03 | Duke University | ORDER AND DISORDER AS A DESIGN PRINCIPLE FOR STIMULI-SENSITIVE BIOPOLYMER NETWORKS |
| JP7282679B2 (ja) * | 2017-09-15 | 2023-05-29 | 株式会社カネカ | 細胞含有試料からの細胞の遊離方法 |
| WO2019147954A1 (en) | 2018-01-26 | 2019-08-01 | Duke University | Albumin binding peptide-drug (aibiped) conjugates and methods of making and using same |
| EP3788343B1 (en) | 2018-04-30 | 2024-03-27 | Duke University | Stimuli-responsive peg-like polymer-based drug delivery platform |
| US11649275B2 (en) | 2018-08-02 | 2023-05-16 | Duke University | Dual agonist fusion proteins |
| US12594347B2 (en) | 2018-09-07 | 2026-04-07 | Duke University | Nanoparticulate drug delivery systems |
| EP3870105A4 (en) * | 2018-10-25 | 2022-08-03 | Endologix LLC | BRANCHING STENTGRAFTS, STENTS AND PROCEDURES |
| WO2020097449A1 (en) * | 2018-11-09 | 2020-05-14 | Figene, Llc | Means and methods of preventing or reversing aging |
| US11512314B2 (en) | 2019-07-12 | 2022-11-29 | Duke University | Amphiphilic polynucleotides |
| WO2021247952A2 (en) * | 2020-06-05 | 2021-12-09 | Duke University | Compositions and methods for augmenting autologous fat grafts |
| US12570949B2 (en) | 2022-12-27 | 2026-03-10 | AVITA Medical Americas, LLC | Systems and devices for wound therapy and related methods of use |
| DE202023002794U1 (de) | 2022-12-27 | 2024-07-15 | AVITA Medical Americas, LLC | Kassette zur Herstellung einer regenerativen epidermalen Suspension |
| USD1112812S1 (en) | 2023-12-22 | 2026-02-10 | AVITA Medical Americas, LLC | Tissue processing cartridge |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5470307A (en) * | 1994-03-16 | 1995-11-28 | Lindall; Arnold W. | Catheter system for controllably releasing a therapeutic agent at a remote tissue site |
| US6238908B1 (en) | 1995-06-07 | 2001-05-29 | Aastrom Biosciences, Inc. | Apparatus and method for maintaining and growth biological cells |
| FR2742662B1 (fr) | 1995-12-21 | 1998-01-23 | Centre Nat Rech Scient | Anticorps anti-idiotypiques du facteur de croissance endotheliale vasculaire et leur utilisation comme medicaments |
| US6280473B1 (en) | 1996-08-19 | 2001-08-28 | Macropore, Inc. | Resorbable, macro-porous, non-collapsing and flexible membrane barrier for skeletal repair and regeneration |
| US5919234A (en) | 1996-08-19 | 1999-07-06 | Macropore, Inc. | Resorbable, macro-porous, non-collapsing and flexible membrane barrier for skeletal repair and regeneration |
| US5980887A (en) * | 1996-11-08 | 1999-11-09 | St. Elizabeth's Medical Center Of Boston | Methods for enhancing angiogenesis with endothelial progenitor cells |
| US6269716B1 (en) | 1998-11-18 | 2001-08-07 | Macropore, Inc. | High-torque resorbable screws |
| JP2002510530A (ja) | 1998-04-07 | 2002-04-09 | マクロポア インコーポレイテッド | 組織案内表面波形部付きのメンブレン |
| US6001642A (en) | 1998-06-29 | 1999-12-14 | Wyle Laboratories, Inc. Life Sciences | Bioreactor and cell culturing processes using the bioreactor |
| KR100979664B1 (ko) * | 1999-03-10 | 2010-09-02 | 유니버시티 오브 피츠버그 오브 더 커먼웰쓰 시스템 오브 하이어 에듀케이션 | 지방 유래 간세포 및 격자 |
| AU3081801A (en) | 1999-11-15 | 2001-05-30 | Chemclean Corporation | Bio-burden visualization system |
| PT1588675E (pt) | 2000-03-10 | 2008-02-29 | Mast Biosurgery Ag | Micromembranas barreira reabsorvíveis para atenuação de tecido cicatricial durante a cicatrização |
| US7094874B2 (en) | 2000-05-26 | 2006-08-22 | Bristol-Myers Squibb Co. | Soluble CTLA4 mutant molecules |
| WO2002028472A2 (en) | 2000-10-04 | 2002-04-11 | James Peter Amis | Non-scatterable, radio-opaque marker for medical imaging applications |
| AU2002256086A1 (en) * | 2001-04-09 | 2002-10-21 | Medtronic, Inc. | Methods of isolating blood components using a centrifuge and uses thereof |
| US20030054331A1 (en) * | 2001-09-14 | 2003-03-20 | Stemsource, Inc. | Preservation of non embryonic cells from non hematopoietic tissues |
| KR100930139B1 (ko) * | 2001-12-07 | 2009-12-07 | 사이토리 테라퓨틱스, 인크. | 처리된 리포애스퍼레이트 세포로 환자를 치료하기 위한시스템 및 방법 |
| US20050250202A1 (en) * | 2002-03-19 | 2005-11-10 | March Keith L | Adipose stromal stem cells for tissue and vascular modification |
| ES2560847T3 (es) * | 2002-07-31 | 2016-02-23 | Yves Saint-Laurent Parfums | Células madre procedentes de tejido adiposo y células diferenciadas procedentes de estas células |
| MXPA05009044A (es) * | 2003-02-20 | 2006-03-17 | Macropore Biosurgery Inc | Metodos para utilizar celulas derivadas de tejido adiposo en el tratamiento de condiciones cardiovasculares. |
-
2004
- 2004-07-01 ES ES04756641.9T patent/ES2633604T3/es not_active Expired - Lifetime
- 2004-07-01 EP EP10183850.6A patent/EP2348103B1/en not_active Expired - Lifetime
- 2004-07-01 CN CNA2004800333682A patent/CN1878470A/zh active Pending
- 2004-07-01 DK DK10183850.6T patent/DK2348103T3/da active
- 2004-07-01 JP JP2006526870A patent/JP5019511B2/ja not_active Expired - Fee Related
- 2004-07-01 SG SG200806960-1A patent/SG146666A1/en unknown
- 2004-07-01 WO PCT/US2004/021480 patent/WO2005034843A2/en not_active Ceased
- 2004-07-01 BR BRPI0414455-4A patent/BRPI0414455A/pt not_active IP Right Cessation
- 2004-07-01 DK DK04756641.9T patent/DK1670315T3/da active
- 2004-07-01 EP EP04756641.9A patent/EP1670315B1/en not_active Expired - Lifetime
- 2004-07-01 AU AU2004280149A patent/AU2004280149A1/en not_active Abandoned
- 2004-07-01 MX MXPA06003088A patent/MXPA06003088A/es not_active Application Discontinuation
- 2004-07-01 CA CA002539346A patent/CA2539346A1/en not_active Abandoned
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10683480B2 (en) | 2013-06-21 | 2020-06-16 | The Regents Of The University Of California | Microfluidic tumor tissue dissociation device and method |
| US11427798B2 (en) | 2013-06-21 | 2022-08-30 | The Regents Of The University Of California | Microfluidic tissue dissociation device and method |
| US10722540B1 (en) | 2016-02-01 | 2020-07-28 | The Regents Of The University Of California | Microfluidic device and method for shear stress-induced transformation of cells |
| US10589268B2 (en) | 2016-06-08 | 2020-03-17 | The Regents Of The University Of California | Method and device for processing tissues and cells |
| US11130127B2 (en) | 2016-06-08 | 2021-09-28 | The Regents Of The University Of California | Method and device for processing tissues and cells |
| US12201978B2 (en) | 2016-06-08 | 2025-01-21 | The Regents Of The University Of California | Method and device for processing tissues and cells |
Also Published As
| Publication number | Publication date |
|---|---|
| EP2348103A3 (en) | 2012-07-11 |
| EP1670315A2 (en) | 2006-06-21 |
| WO2005034843A2 (en) | 2005-04-21 |
| MXPA06003088A (es) | 2006-06-20 |
| SG146666A1 (en) | 2008-10-30 |
| BRPI0414455A (pt) | 2006-11-14 |
| AU2004280149A1 (en) | 2005-04-21 |
| EP2348103B1 (en) | 2019-06-19 |
| CA2539346A1 (en) | 2005-04-21 |
| EP1670315B1 (en) | 2017-04-19 |
| EP2348103A2 (en) | 2011-07-27 |
| JP5019511B2 (ja) | 2012-09-05 |
| ES2633604T3 (es) | 2017-09-22 |
| JP2007505904A (ja) | 2007-03-15 |
| CN1878470A (zh) | 2006-12-13 |
| DK2348103T3 (da) | 2019-09-30 |
| WO2005034843A3 (en) | 2005-06-09 |
| EP1670315A4 (en) | 2006-11-15 |
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