DK169559B1 - Amperometrisk fremgangsmåde til kvantitativ bestemmelse af 1,4-dihydronikotinamid-adenin-dinukleotid (NADH) i opløsning og enzymelektrode til brug ved udøvelse af fremgangsmåden - Google Patents

Amperometrisk fremgangsmåde til kvantitativ bestemmelse af 1,4-dihydronikotinamid-adenin-dinukleotid (NADH) i opløsning og enzymelektrode til brug ved udøvelse af fremgangsmåden Download PDF

Info

Publication number: DK169559B1
Authority: DK; Denmark
Prior art keywords: electrode; nadh; carbon; enzyme; graphite particles
Prior art date: 1987-05-01

Application number

DK731988A

Other languages

Danish (da)

English (en)

Other versions

DK731988A (da

DK731988D0 (da

Inventor

Hugh Peter Bennetto

Gerard Michael Delaney

Jeremy Richard Mason

Christopher Frank Thurston

John Laing Stirling

William Henry Mullen

David Robert Dekeyzer

Original Assignee

Cambridge Life Sciences

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1987-05-01

Filing date

1988-12-30

Publication date

1994-11-28

1988-12-30 Application filed by Cambridge Life Sciences filed Critical Cambridge Life Sciences

1988-12-30 Publication of DK731988D0 publication Critical patent/DK731988D0/da

1989-02-28 Publication of DK731988A publication Critical patent/DK731988A/da

1994-11-28 Application granted granted Critical

1994-11-28 Publication of DK169559B1 publication Critical patent/DK169559B1/da

Links

BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 title claims description 83
229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 title claims description 79
102000004190 Enzymes Human genes 0.000 title claims description 31
108090000790 Enzymes Proteins 0.000 title claims description 31
238000000034 method Methods 0.000 title claims description 26
238000004082 amperometric method Methods 0.000 title 1
229950006238 nadide Drugs 0.000 title 1
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 135
229910052799 carbon Inorganic materials 0.000 claims description 51
BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 48
239000002245 particle Substances 0.000 claims description 40
229910002804 graphite Inorganic materials 0.000 claims description 32
239000010439 graphite Substances 0.000 claims description 32
239000011347 resin Substances 0.000 claims description 26
229920005989 resin Polymers 0.000 claims description 26
229910052697 platinum Inorganic materials 0.000 claims description 21
KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 claims description 16
239000000758 substrate Substances 0.000 claims description 16
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VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims description 7
238000007254 oxidation reaction Methods 0.000 claims description 7
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NBVXSUQYWXRMNV-UHFFFAOYSA-N fluoromethane Chemical group FC NBVXSUQYWXRMNV-UHFFFAOYSA-N 0.000 claims description 6
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MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims 1
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102000003855 L-lactate dehydrogenase Human genes 0.000 description 13
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LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 12
239000000243 solution Substances 0.000 description 12
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IKHGUXGNUITLKF-UHFFFAOYSA-N Acetaldehyde Chemical compound CC=O IKHGUXGNUITLKF-UHFFFAOYSA-N 0.000 description 10
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JQPTYAILLJKUCY-UHFFFAOYSA-N palladium(ii) oxide Chemical compound [O-2].[Pd+2] JQPTYAILLJKUCY-UHFFFAOYSA-N 0.000 description 3
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PHOQVHQSTUBQQK-SQOUGZDYSA-N D-glucono-1,5-lactone Chemical compound OC[C@H]1OC(=O)[C@H](O)[C@@H](O)[C@@H]1O PHOQVHQSTUBQQK-SQOUGZDYSA-N 0.000 description 2
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MUMZUERVLWJKNR-UHFFFAOYSA-N oxoplatinum Chemical compound [Pt]=O MUMZUERVLWJKNR-UHFFFAOYSA-N 0.000 description 2
239000004417 polycarbonate Substances 0.000 description 2
229920000515 polycarbonate Polymers 0.000 description 2
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LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
MASUWVVNWALEEM-UHFFFAOYSA-M 1-methoxy-5-methylphenazin-5-ium;methyl sulfate Chemical compound COS([O-])(=O)=O.C1=CC=C2N=C3C(OC)=CC=CC3=[N+](C)C2=C1 MASUWVVNWALEEM-UHFFFAOYSA-M 0.000 description 1
241000193830 Bacillus <bacterium> Species 0.000 description 1
108010010803 Gelatin Proteins 0.000 description 1
239000013032 Hydrocarbon resin Substances 0.000 description 1
108010093096 Immobilized Enzymes Proteins 0.000 description 1
102000004316 Oxidoreductases Human genes 0.000 description 1
108090000854 Oxidoreductases Proteins 0.000 description 1
LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 1
TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 1
DSVGQVZAZSZEEX-UHFFFAOYSA-N [C].[Pt] Chemical compound [C].[Pt] DSVGQVZAZSZEEX-UHFFFAOYSA-N 0.000 description 1
XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 description 1
239000002253 acid Substances 0.000 description 1
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QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
HWYNRVXFYFQSID-UHFFFAOYSA-M benzo[a]phenoxazin-9-ylidene(dimethyl)azanium;chloride Chemical compound [Cl-].C1=CC=C2C(N=C3C=CC(C=C3O3)=[N+](C)C)=C3C=CC2=C1 HWYNRVXFYFQSID-UHFFFAOYSA-M 0.000 description 1
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IDLFZVILOHSSID-OVLDLUHVSA-N corticotropin Chemical compound C([C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(N)=O)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)NC(=O)[C@@H](N)CO)C1=CC=C(O)C=C1 IDLFZVILOHSSID-OVLDLUHVSA-N 0.000 description 1
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235000011852 gelatine desserts Nutrition 0.000 description 1
235000012209 glucono delta-lactone Nutrition 0.000 description 1
239000007770 graphite material Substances 0.000 description 1
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210000004185 liver Anatomy 0.000 description 1
UKVIEHSSVKSQBA-UHFFFAOYSA-N methane;palladium Chemical compound C.[Pd] UKVIEHSSVKSQBA-UHFFFAOYSA-N 0.000 description 1
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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/40—Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/817—Enzyme or microbe electrode

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Immunology (AREA)
Organic Chemistry (AREA)
Molecular Biology (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Biotechnology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Analytical Chemistry (AREA)
Hematology (AREA)
Biomedical Technology (AREA)
Physics & Mathematics (AREA)
Microbiology (AREA)
Bioinformatics & Cheminformatics (AREA)
Urology & Nephrology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
General Engineering & Computer Science (AREA)
General Physics & Mathematics (AREA)
Medicinal Chemistry (AREA)
Pathology (AREA)
Genetics & Genomics (AREA)
Biophysics (AREA)
Cell Biology (AREA)
Food Science & Technology (AREA)
Chemical Kinetics & Catalysis (AREA)
Electrochemistry (AREA)
Sustainable Development (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Electrodes For Compound Or Non-Metal Manufacture (AREA)

DK731988A 1987-05-01 1988-12-30 Amperometrisk fremgangsmåde til kvantitativ bestemmelse af 1,4-dihydronikotinamid-adenin-dinukleotid (NADH) i opløsning og enzymelektrode til brug ved udøvelse af fremgangsmåden DK169559B1 (da)

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date	Title
GB878710472A GB8710472D0 (en)	1987-05-01	1987-05-01	Amperometric method
GB8710472		1987-05-01
GB8800338		1988-04-29
PCT/GB1988/000338 WO1988008447A1 (fr)	1987-05-01	1988-04-29	Procede amperometrique servant a la determination quantitative de dinucleotide de 1,4-dihydronicotinamide-adenine en solution

Publications (3)

Publication Number	Publication Date
DK731988D0 DK731988D0 (da)	1988-12-30
DK731988A DK731988A (da)	1989-02-28
DK169559B1 true DK169559B1 (da)	1994-11-28

Family

ID=10616757

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DK731988A DK169559B1 (da)	1987-05-01	1988-12-30	Amperometrisk fremgangsmåde til kvantitativ bestemmelse af 1,4-dihydronikotinamid-adenin-dinukleotid (NADH) i opløsning og enzymelektrode til brug ved udøvelse af fremgangsmåden

Country Status (18)

Country	Link
US (1)	US5122456A (fr)
EP (2)	EP0289344B1 (fr)
JP (2)	JP2528956B2 (fr)
KR (1)	KR960001501B1 (fr)
AU (2)	AU1686188A (fr)
CA (1)	CA1304127C (fr)
DE (1)	DE3882078T2 (fr)
DK (1)	DK169559B1 (fr)
ES (1)	ES2058271T3 (fr)
FI (1)	FI92221C (fr)
GB (3)	GB8710472D0 (fr)
HU (1)	HU205625B (fr)
IE (1)	IE60238B1 (fr)
IL (2)	IL86222A0 (fr)
MX (1)	MX167769B (fr)
NO (1)	NO179953C (fr)
RU (1)	RU1806187C (fr)
WO (2)	WO1988008447A1 (fr)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB8710472D0 (en) *	1987-05-01	1987-06-03	Cambridge Life Sciences	Amperometric method
GB8817997D0 (en) *	1988-07-28	1988-09-01	Cambridge Life Sciences	Enzyme electrodes & improvements in manufacture thereof
DE3932247A1 (de) *	1989-09-27	1991-04-04	Biotechnolog Forschung Gmbh	Elektrodenmaterial, elektroden, verfahren zur herstellung und verwendung der elektrode
TW279133B (fr) *	1990-12-13	1996-06-21	Elan Med Tech
AT397513B (de) *	1992-12-15	1994-04-25	Avl Verbrennungskraft Messtech	Amperometrische enzymelektrode
US5429735A (en) *	1994-06-27	1995-07-04	Miles Inc.	Method of making and amperometric electrodes
IE72524B1 (en) *	1994-11-04	1997-04-23	Elan Med Tech	Analyte-controlled liquid delivery device and analyte monitor
DE4442253A1 (de) *	1994-11-28	1996-05-30	Bayer Corp N D Ges D Staates I	Elektrochemischer Enzymbiosensor
US8075760B2 (en)	1995-06-19	2011-12-13	Lifescan, Inc.	Electrochemical cell
FR2743575B1 (fr) *	1996-01-17	1998-03-13	Univ Toulouse	Procede perfectionne de modification en surface d'une electrode pour la realisation d'un reaction electrochimique et electrodes obtenues
DE69730612T2 (de) *	1996-11-07	2005-01-27	Cambridge Sensors Ltd., Godmanchester	Elektroden und ihre verwendung in assays
AU8031898A (en)	1997-06-16	1999-01-04	Elan Medical Technologies Limited	Methods of calibrating and testing a sensor for (in vivo) measurement of an analyte and devices for use in such methods
KR100491316B1 (ko) *	2002-05-29	2005-05-24	삼성엔지니어링 주식회사	망간이 고정된 흑연 또는 뉴트랄 레드가 결합된 흑연을포함하는 ｎａｄ(ｐ)+ 또는 ｎａｄ(ｐ)ｈ의 직접 환원또는 산화용 전극
JP5273680B2 (ja)	2007-09-18	2013-08-28	国立大学法人東京農工大学	酵素電極
JP5362406B2 (ja) *	2009-03-25	2013-12-11	三洋電機株式会社	燃料電池
JP5930810B2 (ja)	2011-04-26	2016-06-08	アークレイ株式会社	分析用具
CN103650203B (zh) *	2011-07-07	2017-03-01	丰田自动车株式会社	二次电池
WO2020148414A1 (fr)	2019-01-18	2020-07-23	Frieslandcampina Nederland B.V.	Produit fromager moulé
CN116698945A (zh) *	2023-06-09	2023-09-05	深圳津合生物有限公司	一种酶电极系统及其应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4044193A (en) *	1971-06-16	1977-08-23	Prototech Company	Finely particulated colloidal platinum compound and sol for producing the same, and method of preparation of fuel cell electrodes and the like employing the same
SU593439A1 (ru) *	1975-08-04	1980-10-07	Ордена Трудового Красного Знамени Институт Химических Наук Ан Казахской Сср	Способ получени электропроводных фермент-ковакторных систем
US4166143A (en) *	1977-01-24	1979-08-28	Prototech Company	Control of the interaction of novel platinum-on-carbon electrocatalysts with fluorinated hydrocarbon resins in the preparation of fuel cell electrodes
US4321123A (en) *	1978-04-21	1982-03-23	Matsushita Electric Industrial Co., Ltd.	Coenzyme immobilized electrode
JPS5816695B2 (ja) *	1978-04-24	1983-04-01	松下電器産業株式会社	酵素電極
JPS584982B2 (ja) *	1978-10-31	1983-01-28	松下電器産業株式会社	酵素電極
US4293396A (en) *	1979-09-27	1981-10-06	Prototech Company	Thin carbon-cloth-based electrocatalytic gas diffusion electrodes, and electrochemical cells comprising the same
US4478696A (en) *	1982-07-21	1984-10-23	Prototech Company	Ionizable reducing and oxidizing gaseous supply means and process for catalytic barriers and electrodes
GB8606824D0 (en) *	1986-03-19	1986-04-23	Univ Strathclyde	Biochemical detector
GB8612861D0 (en) *	1986-05-27	1986-07-02	Cambridge Life Sciences	Immobilised enzyme biosensors
GB8710472D0 (en) *	1987-05-01	1987-06-03	Cambridge Life Sciences	Amperometric method

1987
- 1987-05-01 GB GB878710472A patent/GB8710472D0/en active Pending
1988
- 1988-04-28 IL IL86222A patent/IL86222A0/xx unknown
- 1988-04-28 IL IL86223A patent/IL86223A/xx not_active IP Right Cessation
- 1988-04-28 IE IE127488A patent/IE60238B1/en not_active IP Right Cessation
- 1988-04-29 KR KR1019880701786A patent/KR960001501B1/ko not_active Expired - Lifetime
- 1988-04-29 JP JP63503840A patent/JP2528956B2/ja not_active Expired - Lifetime
- 1988-04-29 HU HU883273A patent/HU205625B/hu unknown
- 1988-04-29 US US07/295,035 patent/US5122456A/en not_active Expired - Lifetime
- 1988-04-29 AU AU16861/88A patent/AU1686188A/en not_active Abandoned
- 1988-04-29 DE DE88303921T patent/DE3882078T2/de not_active Expired - Lifetime
- 1988-04-29 AU AU16862/88A patent/AU612366B2/en not_active Expired
- 1988-04-29 EP EP88303921A patent/EP0289344B1/fr not_active Expired - Lifetime
- 1988-04-29 WO PCT/GB1988/000338 patent/WO1988008447A1/fr not_active Ceased
- 1988-04-29 GB GB8810181A patent/GB2204698B/en not_active Expired - Lifetime
- 1988-04-29 GB GB8810180A patent/GB2204415B/en not_active Expired - Lifetime
- 1988-04-29 JP JP63503839A patent/JPH01503409A/ja active Pending
- 1988-04-29 WO PCT/GB1988/000337 patent/WO1988008446A1/fr not_active Ceased
- 1988-04-29 ES ES88303921T patent/ES2058271T3/es not_active Expired - Lifetime
- 1988-04-29 EP EP88303922A patent/EP0289345A1/fr not_active Withdrawn
- 1988-05-02 MX MX011347A patent/MX167769B/es unknown
- 1988-05-02 CA CA000565696A patent/CA1304127C/fr not_active Expired - Lifetime
- 1988-12-30 DK DK731988A patent/DK169559B1/da not_active IP Right Cessation
- 1988-12-30 FI FI886050A patent/FI92221C/fi not_active IP Right Cessation
- 1988-12-30 RU SU884613363A patent/RU1806187C/ru active
- 1988-12-30 NO NO885836A patent/NO179953C/no not_active IP Right Cessation

Also Published As

Publication number	Publication date
GB8810180D0 (en)	1988-06-02
IE881274L (en)	1988-11-01
GB8810181D0 (en)	1988-06-02
HU205625B (en)	1992-05-28
EP0289345A1 (fr)	1988-11-02
HUT50863A (en)	1990-03-28
DK731988A (da)	1989-02-28
NO885836L (no)	1988-12-30
AU612366B2 (en)	1991-07-11
FI92221C (fi)	1994-10-10
WO1988008447A1 (fr)	1988-11-03
EP0289344A1 (fr)	1988-11-02
KR890701721A (ko)	1989-12-21
DE3882078D1 (de)	1993-08-05
JPH01503409A (ja)	1989-11-16
IE60238B1 (en)	1994-06-15
GB8710472D0 (en)	1987-06-03
WO1988008446A1 (fr)	1988-11-03
FI92221B (fi)	1994-06-30
IL86223A (en)	1993-01-14
JPH01503410A (ja)	1989-11-16
NO885836D0 (no)	1988-12-30
FI886050A7 (fi)	1988-12-30
US5122456A (en)	1992-06-16
NO179953C (no)	1997-01-15
CA1304127C (fr)	1992-06-23
AU1686188A (en)	1988-12-02
GB2204698B (en)	1991-04-17
GB2204698A (en)	1988-11-16
IL86223A0 (en)	1988-11-15
GB2204415B (en)	1991-05-08
IL86222A0 (en)	1988-11-15
KR960001501B1 (ko)	1996-01-31
AU1686288A (en)	1988-12-02
EP0289344B1 (fr)	1993-06-30
DE3882078T2 (de)	1994-01-20
DK731988D0 (da)	1988-12-30
JP2528956B2 (ja)	1996-08-28
NO179953B (no)	1996-10-07
ES2058271T3 (es)	1994-11-01
GB2204415A (en)	1988-11-09
MX167769B (es)	1993-04-12
RU1806187C (ru)	1993-03-30

Legal Events

Date	Code	Title	Description
1994-11-28	B1	Patent granted (law 1993)
2008-05-19	PUP	Patent expired

Publication	Publication Date	Title
DK169559B1 (da)	1994-11-28	Amperometrisk fremgangsmåde til kvantitativ bestemmelse af 1,4-dihydronikotinamid-adenin-dinukleotid (NADH) i opløsning og enzymelektrode til brug ved udøvelse af fremgangsmåden
EP0247850B1 (fr)	1993-04-21	Electrodes à enzymes immobilisées
EP0352925B1 (fr)	1994-12-28	Electrodes enzymatiques et leur fabrication
Hart et al.	1994	Screen‐printed voltammetric and amperometric electrochemical sensors for decentralized testing
EP0343203B1 (fr)	1994-05-25	Electrodes a enzyme immobilisee et procede de reduction de la sensibilite a l'alcool
ITMI951441A1 (it)	1997-01-05	Nuovi biosensori elettrochimici basati su nuovi trasduttori compositi
JPS6239900B2 (fr)	1987-08-25
Yabuki et al.	1997	d‐Fructose sensing electrode based on electron transfer of d‐fructose dehydrogenase at colloidal gold‐enzyme modified electrode
Reddy et al.	1994	Selective membranes for the construction and optimization of an amperometric oxalate enzyme electrode
Loughran et al.	1995	Amperometric detection of histamine at a quinoprotein dehydrogenase enzyme electrode
JPH0721479B2 (ja)	1995-03-08	酵素電極及びこれを用いたセンサ、定量分析方法
Ikeda et al.	1998	Electrochemical monitoring of in vivo reconstitution of glucose dehydrogenase in Escherichia coli cells with externally added pyrroloquinoline quinone