DK2316929T3 - Maltogene alfa-amylasevarianter - Google Patents

Maltogene alfa-amylasevarianter Download PDF

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Publication number
DK2316929T3
DK2316929T3 DK10181858.1T DK10181858T DK2316929T3 DK 2316929 T3 DK2316929 T3 DK 2316929T3 DK 10181858 T DK10181858 T DK 10181858T DK 2316929 T3 DK2316929 T3 DK 2316929T3
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asn
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DK10181858.1T
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Joel Robert Cherry
Allan Svendsen
Carsten Andersen
Lars Beier
Torben Peter Frandsen
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Novozymes As
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
    • A21D8/00—Methods for preparing or baking dough
    • A21D8/02—Methods for preparing dough; Treating dough prior to baking
    • A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Detergent Compositions (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Noodles (AREA)

Claims (7)

1. Polypeptid, som: a) har maltogen alfa-amylaseaktivitet; b) udviser mindst 70% identitet med SEQ ID NO: 1; c) omfatter en aminosyremodifikation i sammenligning med SEQ ID NO: 1 i en position svarende til rest T288; og d) har en ændret pH-afhængig aktivitetsprofil i sammenligning med polypeptidet ifølge SEQ ID NO: 1.
2. Polypeptid ifølge krav 1, hvor modifikationen i sammenligning med SEQ ID NO: 1 omfatter T288E, T288K eller T288R.
3. Nukleinsyresekvens, som koder for polypeptidet ifølge et hvilket som helst af kravene 1-2, hvilken nukleinsyresekvens fortrinsvis er operativt forbundet til en eller flere kontrolsekvenser, der styrer ekspressionen af varianten i en egnet ekspressionsvært.
4. Rekombinant ekspressionsvektor, som omfatter nukleinsyresekvensen ifølge krav 3, en promotor og transskriptions- og translationsstopsignaler, og som fortrinsvis endvidere omfatter en selekterbar markør.
5. Transformeret værtscelle, som omfatter nukleinsyresekvensen ifølge krav 3 eller vektoren ifølge krav 4.
6. Fremgangsmåde til fremstilling af polypeptidet ifølge et hvilket som helst af kravene 1-2, hvilken fremgangsmåde omfatter: a) dyrkning af den transformerede værtscelle ifølge krav 6 under betingelser, som er befordrende for ekspression af varianten; og b) indvinding af varianten.
7. Fremgangsmåde til fremstilling af en dej eller et bagt produkt fremstillet af dej, hvilken fremgangsmåde omfatter tilsætning af polypeptidet ifølge et hvilket som helst af kravene 1-2 til dejen i en mængde, som er effektiv til at forsinke ældningen af brødet.
DK10181858.1T 1998-02-27 1999-02-26 Maltogene alfa-amylasevarianter DK2316929T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK26998 1998-02-27
EP99904736A EP1058724B1 (en) 1998-02-27 1999-02-26 Maltogenic alpha-amylase variants

Publications (1)

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DK2316929T3 true DK2316929T3 (da) 2016-07-25

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ID=8091645

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Application Number Title Priority Date Filing Date
DK10181858.1T DK2316929T3 (da) 1998-02-27 1999-02-26 Maltogene alfa-amylasevarianter
DK99904736.8T DK1058724T3 (en) 1998-02-27 1999-02-26 Maltogene alpha-amylase varianter
DK10181883.9T DK2305799T3 (da) 1998-02-27 1999-02-26 Maltogene alfa-amylasevarianter

Family Applications After (2)

Application Number Title Priority Date Filing Date
DK99904736.8T DK1058724T3 (en) 1998-02-27 1999-02-26 Maltogene alpha-amylase varianter
DK10181883.9T DK2305799T3 (da) 1998-02-27 1999-02-26 Maltogene alfa-amylasevarianter

Country Status (14)

Country Link
US (1) US6162628A (da)
EP (3) EP1058724B1 (da)
JP (2) JP4672864B2 (da)
CN (2) CN1292028B (da)
AT (1) ATE490312T1 (da)
AU (1) AU757935B2 (da)
BR (1) BRPI9908281B1 (da)
CA (2) CA2321595C (da)
DE (1) DE69942995D1 (da)
DK (3) DK2316929T3 (da)
NZ (1) NZ505820A (da)
RU (1) RU2258739C2 (da)
TR (1) TR200002498T2 (da)
WO (1) WO1999043794A1 (da)

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