DK2420570T3 - Arabinofuranosidaseenzymer, nucleinsyrer, som koder for dem, og fremgangsmåder til at fremstille og anvende dem - Google Patents

Arabinofuranosidaseenzymer, nucleinsyrer, som koder for dem, og fremgangsmåder til at fremstille og anvende dem Download PDF

Info

Publication number
DK2420570T3
DK2420570T3 DK11007477T DK11007477T DK2420570T3 DK 2420570 T3 DK2420570 T3 DK 2420570T3 DK 11007477 T DK11007477 T DK 11007477T DK 11007477 T DK11007477 T DK 11007477T DK 2420570 T3 DK2420570 T3 DK 2420570T3
Authority
DK
Denmark
Prior art keywords
polypeptide
nucleic acid
sequence
seq
optionally
Prior art date
Application number
DK11007477T
Other languages
English (en)
Inventor
Kevin A Gray
Lishan Zhao
Michelle H Cayouette
Original Assignee
Bp Corp North America Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bp Corp North America Inc filed Critical Bp Corp North America Inc
Application granted granted Critical
Publication of DK2420570T3 publication Critical patent/DK2420570T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01006—Endo-1,3(4)-beta-glucanase (3.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01039—Glucan endo-1,3-beta-D-glucosidase (3.2.1.39)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01055—Alpha-N-arabinofuranosidase (3.2.1.55)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/249921—Web or sheet containing structurally defined element or component

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)
  • Peptides Or Proteins (AREA)

Claims (19)

1. Isoleret, syntetisk eller rekombinant nucleinsyre omfattende (a) en nucleinsyresekvens med mindst 70% 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% eller mere eller komplet (100%) sekvensidentitet til SEQ ID NO: 103, hvor nucleinsy-ren, som koder for et polypeptid, har en arabinofuranosidaseaktivitet, og eventuelt er sekvensidentiteterne bestemt ved analyse med en sekvenssammenligningsalgoritme eller ved en visuel inspicering, eller (b) en nucleinsyresekvens, som hybridiserer ved stringente betingelser til en nucleinsyre omfattende SEQ ID NO: 103, hvor nucleinsyren koder for et polypeptid med en arabinofuranosidaseaktivitet, og har mindst 70% identitet med SEQ ID NO: 103, og de stringente betingelser indbefatter et vasketrin omfattende en vask med 0,2 X SSC ved en temperatur på ca. 65°C i ca. 15 minutter, (c) en nucleinsyresekvens, som koder for et polypeptid med mindst 70% identitet med sekvensen i SEQ ID NO: 104, hvor polypeptidet har arabinofuranosidaseaktivitet, (d) nucleinsyren i (a), (b) eller (c), som koder for et polypeptid med en arabinofuranosidaseaktivitet, men mangler en signalsekvens eller et carbohy-dratbindingsmodul, (e) nucleinsyren i (a), (b), (c) eller (d), som koder for et polypeptid med en arabinofuranosidaseaktivitet, men har en heterolog sekvens, hvor den heterologe sekvens eventuelt omfatter en heterolog signalsekvens, et carbohydratbindingsmodul, et katalytisk domæne (CD) eller en kombination heraf, og eventuelt den heterologe signalsekvens, carbohy-dratbindingsmodulet eller det katalytiske domæne (CD) er afledt fra en anden oligomerase, cellulase eller cellulytisk enzym, eller en non-oligomera-se, -cellulase eller -cellulytisk enzym, eller (f) en nucleinsyresekvens fuldstændig komplementær til (a), (b), (c), (d) eller (e).
2. Ekspressionskassette, vektor eller kloningsvehikel omfattende en nuclein-syre omfattende sekvensen i krav 1, hvor eventuelt kloningsvehiklen omfatter en viral vektor, et plasmid, en phag, et phagemid, et cosmid, et fosmid, en bak-teriophag eller et kunstigt kromosom, og hvor eventuelt kloningsvehiklen omfatter en viral vektor omfattende en adenovirusvektor, en retroviral vektor eller en adenoassocieret viral vektor, eller hvor eventuelt kloningsvehiklen omfatter et bakterielt kunstigt kromosom (BAC), et plasmid, en bakteriophag P1-afledt vektor (PAC), et kunstigt gærkromosom (YAC) eller et kunstigt patterdyrskromosom (MAC).
3. Transformeret celle omfattende en nucleinsyre omfattende sekvensen i krav 1, eller en vektor eller kloningsvehikel som anført i krav 2, hvor eventuelt cellen er en bakteriel celle, en pattedyrscelle, en svampecelle, en gærcelle, en insektcelle eller en plantecelle, og hvor den transformerede celle producerer et polypeptid kodet for af nucleinsyren ifølge krav 1, og som har arabinofurano-sidaseaktivitet.
4. Transgent ikke-humant dyr, plante, plantedel eller plantefrø omfattende sekvensen i krav 1 eller en vektor eller kloningsvehikel ifølge krav 2, hvor eventuelt det transgene ikke-humane dyr er en mus eller en rotte, og hvor det ikke-humane dyrs, plantes, plantedels eller plantefrøs transformerede celle fremstiller et polypeptid kodet for af nucleinsyren i krav 1, og har arabinofuranosidase-aktivitet.
5. Isoleret, syntetisk eller rekombinant polypeptid (i) med en aminosyresekvens, som har mindst 70%, 71 %, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81 %, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91 %, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% eller mere eller 100% sekvensidentitet til SEQ ID NO: 104, hvor polypepti-det har en arabinofuranosidaseaktivitet, hvor sekvensidentiteterne eventuelt bestemmes ved analyse med en sekvenssammenligningsalgoritme eller ved visuel inspektion, og eventuelt er sekvenssammenligningsalgoritmen en BLAST version 2.2.2-algoritme, hvor en filtreringsindstilling er sat til blastall-p blastp -d, ”nr pataa”-F F, og alle andre valg er sat til default, (ii) med en aminosyresekvens kodet for af en nucleinsyre ifølge krav 1, hvor polypeptidet har en arabinofuranosidaseaktivitet eller har immunogen aktivitet derved, at den er i stand til at generere et antistof, som specifikt binder til et polypeptid med sekvensen i SEQ ID NO: 104, (iii) polypeptidet i (i) eller (ii) med en arabinofuranosidaseaktivitet, men uden en signalsekvens, (iv) polypeptidet fra (i), (ii) eller (iii) med en arabinofuranosidaseaktivitet, men som har en heterolog sekvens, hvor den heterologe sekvens ifølge krav omfatter en heterolog signalsekvens, et carbohydratbindingsmodul, et katalytisk domæne (CD) eller en kombination heraf, og den heterologe signalsekvens, carbohydratbindings-modulet eller det katalytiske domæne (CD) stammer eventuelt fra en anden oligomerase, cellulase eller cellulytisk enzym, eller en non-oligomera-se, -cellulase eller non-cellulytisk enzym, eller (v) polypeptidet fra (i), (ii), (iii) eller (iv), hvor polypeptidet er glycosyleret.
6. Isoleret, syntetisk eller rekombinant polypeptid med en aminosyresekvens fra SEQ ID NO: 104.
7. Polypeptid ifølge krav 6 med arabinofuranosidaseaktivitet, en signalsekvens og et carbohydratbindingsmodul.
8. Fremgangsmåde til fremstilling af et rekombinant polypeptid omfattende trinnene: (a) tilvejebringelse af en nucleinsyre ifølge krav 1, og (b) ekspression af nucleinsyren i trin (a) ved betingelser, som tillader ekspression af polypeptidet, og derved producere et rekombinant polypeptid, hvor eventuelt fremgangsmåden yderligere omfatter at transformere en værtscelle med nucleinsyren fra trin (a) fulgt at ekspression af nucleinsyren fra trin (a) og derved producere et rekombinant polypeptid i en transformeret celle.
9. Fremgangsmåde til at generere en variant af en nucleinsyre, som koder for et polypeptid med en arabinofuranosidaseaktivitet omfattende trinnene: (a) tilvejebringelse af en templatnucleinsyre omfattende sekvensen ifølge krav 1, og (b) modificering, deletering eller addering af en eller flere nucleotider i tem-platsekvensen, eller en kombination heraf for at generere en variant af templatnucleinsyren, hvor fremgangsmåden eventuelt omfatter at udtrykke nucleinsyrevarianten for at generere en oligomerasevariant og/eller cellulasepolypeptidvariant, og eventuelt indføres modifikationerne, additionerne eller deleteringerne ved en fremgangsmåde omfattende fejlbehæftet PCR, shuffling, oligonucleotidrettet mutagenese, samlings-PCR, seksuel PCR-mutagenese, in vivo mutagenese, kassettemutagenese, rekursiv gruppemutagenese, eksponentiel gruppemutage-nese, stedsspecifik mutagenese, gensamling, Gene Site Saturation Mutagenesis (GSSM), syntetisk ligeringsgensamling (SLR), rekombination, rekursiv sekvensrekombination, phosphothiat-modificeret DNA mutagenese, uracilholdig templatmutagenese, gapped dupleks mutagenese, punktfejlparringsreparations-mutagenese, reparationsdeficient værtsstammemutagenese, kemisk mutagenese, radiogenisk mutagenese, deleteringsmutagenese, restriktions/selekterings-mutagenese, restriktionsoprensningsmutagenese, kunstig gensyntese, homo-mutagenese, kimærisk nucleinsyre multimerskabelse og en kombination heraf, og hvor fremgangsmåden eventuelt iterativt gentages indtil en oligomerase og/-eller en cellulase med en ændret eller forskellig aktivitet, en ændret eller forskellig stabilitet, en ændret kodonanvendelse eller en højere eller lavere grad af messageekspressioner sammenlignet med et polypeptid kodet af templatnucleinsyren fremstilles.
10. Fremgangsmåde til at modificere kodoner i en nucleinsyrer, som koder for et arabinofuranosidasepolypeptid, hvilken fremgangsmåde omfatter følgende trin: (a) tilvejebringelse af en nucleinsyre, som koder for et polypeptid ifølge krav 5, og (b) identificering af en kodon i nucleinsyren i trin (a) og erstatning af den med en forskellig kodon, som koder for den samme aminosyre som den erstattede kodon og derved modificerer kodoner i en nucleinsyre, som koder for en cellulase.
11. Kimærisk polypeptid omfattende et første domæne og mindst et andet domæne, hvor det første domæne omfatter et enzym som anført i krav 5, og det andet domæne omfatter et heterologt eller modificeret carbohydratbindingsdo-mæne eller et heterologt eller modificeret dockerin-domæne, og carbohydratbin-dingsdomænet eventuelt er et cellulosebindende modul (CBM), eller et lignin-bindende domæne, og det andet domæne eventuelt vedhæftet tæt ved enzymets katalytiske domæne, og det andet domæne er eventuelt hæftet omtrent ved C-terminalen af enzymets katalytiske domæne.
12. Sammensætning eller fremstillingsprodukt omfattende (a) en blanding (eller ’’cocktail”) af hemicellulose- og cellulose-hydrolyse-rende enzymer, hvor de cellulosehydrolyserende enzymer omfatter mindst et af hver af en endoglucanase, cellobiohydrolase I (CBHI), cellobiohydro-lase 11 (CBH11) og β-glucosidase, og de hemicellulosehydrolyserende enzymer omfatter mindst et af hver af en xylanase, β- xylosidase og arabino-furanosidase, hvor mindst en arabinofuranosidase er et polypeptid ifølge krav 5 eller et polypeptid kodet for af en nucleinsyre ifølge krav 1, (b) en blanding (eller ’’cocktail”) af hemicellulose- og cellulosehydrolyserende enzymer omfattende mindst et af hver af en endoglucanase, oligomera-se, cellobiohydrolase I (CBHI), cellobiohydrolase II (CBHII), arabinofuranosidase og xylanase, hvor oligomerasen eventuelt er en oligomerase-1 eller β-g lu cos id ase eller oligomerasen eventuelt er en oligomerase-2 eller β-xy-losidase, og hvor mindst en arabinofuranosidase er et polypeptid ifølge krav 5 eller et polypeptid kodet for af en nucleinsyre ifølge krav 1, (c) en blanding (eller ’’cocktail”) af hemicellulose- og cellulosehydrolyse-rende enzymer omfattende mindst en af hver af endoglucanase, en cello-biohydrolase I (CBHI), en cellobiohydrolase II (CBHII), en arabinofuranosidase, en xylanase, en oligomerase-1 (en β-glucosidase), og en oligomerase-2 eller β-xylosidase, og hvor mindst en arabinofuranosidase er et polypeptid ifølge krav 5 eller et polypeptid kodet for af en nucleinsyre ifølge krav 1, (d) et polypeptid med cellobiohydrolase l-aktivitet og et polypeptid med ara-binofuranosidaseaktivitet, hvor mindst et polypeptid med cellobiohydrolase l-aktivitet er SEQ ID NO: 34 eller SEQ ID NO: 46, og hvor mindst en arabinofuranosidase er et polypeptid ifølge krav 5 eller et polypeptid kodet for af en nucleinsyre ifølge krav 1, hvor eventuelt (i) endoglucanasen omfatter SEQ ID NO: 106, cellobiohydrolase I omfatter SEQ ID NO: 34 eller SEQ ID NO: 46, cellobiohydrolase II omfatter SEQ ID NO: 98, β-glucosidasen omfatter SEQ ID NO: 94, xylanasen omfatter SEQ ID NO: 100, SEQ ID NO: 102 eller SEQ ID NO: 524, β-xylosodasen omfatter SEQ ID NO: 96, en yderligere arabinofuranosidase, såfremt den er til stede, omfatter SEQ ID NO: 92 eller en vilkårlig kombination heraf, hvor SEQ ID NO: 106 eventuelt omfatter et yderligere carbohydratbindingsdo-mæne, (ii) blandingen omfatter en endoglucanase omfattende SEQ ID NO: 106, en oligomerase-1 (en β-glucosidase) omfattende SEQ ID NO: 522, en cellobiohydrolase I (CBHI) omfattende SEQ ID NO: 34 eller SEQ ID NO: 46, en cellobiohydrolase II (CBHII) omfattende SEQ ID NO: 98, en yderligere eventuel arabinofuranosidase, hvis til stede, omfattende SEQ ID NO: 92, en oligomerase-2 (en β-xylosidase) omfattende SEQ ID NO: 520, og en xy- lanase omfattende SEQ ID NO: 102, SEQ ID NO: 524 og/eller SEQ ID NO: 100, eller (iii) sammensætningen eller fremstillingsproduktet omfattende yderligere et biomassemateriale, hvor biomassematerialet eventuelt omfatter et lignocel-lulosemateriale afledt fra en landbrugsmæssig afgrøde, eller biomassematerialet er et biprodukt af en fødevare- eller en foderstofproduktion, eller biomassematerialet er et lignocellulosespildprodukt, eller biomassematerialet er en planteremanens eller affaldspapir eller et affaldspapirprodukt, eller biomassematerialet omfatter en planteremanens, og planteremanensen eventuelt omfatter stængler, blade, avner, majskolber, majsblade, hø, træ, træflis, træpulp og/eller savsmuld, og papiraffaldet omfatter eventuelt bortkastet og brugt fotokopieringspapir, computerprinterpapir, notesblokpapir, notesbogpapir, maskinskrivningspapir, aviser, magasiner, pap- og papirbaserede emballagematerialer.
13. Sammensætning eller fremstillingsprodukt omfattende en blanding af enzymer omfattende: (a) SEQ ID NO: 106, en cellobiohydrolase I (CBHI), en cellobiohydrolase II (CBHII) og en arabinofuranosidase i SEQ ID NO: 104, (b) blandingen fra (a), hvor CBHI er SEQ ID NO: 46 eller SEQ ID NO: 34, (c) blandingen fra (a) eller (b), hvor CBHII er SEQ ID NO: 98, (d) blandingen fra (a), (b) eller (c) omfattende en yderligere arabinofuranosidase i SEQ ID NO: 92, (e) blandingen fra (a), (b), (c) eller (d) omfattende yderligere en xylanase, (f) blandingen fra (e), hvor xylanasen er SEQ ID NO: 100, SEQ ID NO: 102 eller SEQ ID NO: 524 eller en kombination heraf, (g) blandingen fra (a), (b), (c), (d), (e) eller (f) omfattende yderligere en oligomerase, (h) blandingen fra (g), hvor oligomerasen er SEQ ID NO: 520 eller SEQ ID NO: 522 eller en kombination heraf, (i) blandingen fra (a), (b), (c), (d), (e), (f), (g) eller (h) omfattende yderligere mindst en af SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 264, SEQ ID NO: 440 eller SEQ ID NO: 442 eller en kombination heraf, eller (j) blandingen fra (a), (b), (c), (d), (e), (f), (g), (h) eller (i) yderligere omfattende en endoglucanase, hvor endoglucanasen eventuelt omfatter SEQ ID NO: 108, SEQ ID NO: 112, SEQ ID NO: 114 eller SEQ ID NO: 116.
14. Enzymgruppe eller ’’cocktail” til depolymerisering af cellulose- og hæmicel-lulosepolymerer til metaboliseringsdygtige carbongrupper omfattende et po-lypeptid som anført i krav 5 eller et polypeptid kodet for af en nucleinsyre som anført i krav 1.
15. Sammensætning omfattende et polypeptid som anført i krav 5 eller et polypeptid kodet for af en nucleinsyre som anført i krav 1 eller en sammensætning eller et fremstillingsprodukt som anført i et vilkårligt af kravene 12 eller 13 eller en enzymgruppe ifølge krav 14, hvor sammensætningen eventuelt er en farmaceutisk sammensætning, en detergentsammensætning, en kontaktlinseopløsning, en spildbehandlingssammensætning, et desinficerende middel, et bioforsvarsmiddel eller et biodetyficeringsmiddel, eller hvor sammensætningen eventuelt er et brændstof, hvor sammensætningen eventuelt er en alkohol, hvor alkoholen eventuelt er ethanol, eller hvor sammensætningen eventuelt er en biomasse eller et biomassemateriale, et papir, papiraffald, genanvendt papir, papirpulp, et papirprodukt, træ, træprodukt, træpulp, tekstil, væv, garn eller fibre, eller hvor sammensætningen eventuelt er en cellulose- eller en celluloseafledt sammensætning, eller hvor eventuelt sammensætningen er en drikkevare, en fødevare, et foderstof, et mejeriprodukt eller et ernæringsmæssigt supplement, eller hvor eventuelt fødevaren er en dej, et brød eller et bagt produkt, eller hvor eventuelt drikkevaren, fødevaren, foderet eller det ernæringsmæssige supplement er til et dyr, eller hvor eventuelt biomassematerialet omfatter et lignocellu-losemateriale stammende fra en landbrugsmæssig afgrøde, eller biomassematerialet er et biprodukt af en fødevareproduktion eller en foderstofproduktion, eller biomassematerialet er et lignocelluloseaffaldsprodukt, eller biomassematerialet er en planteremanens eller et affaldspapir eller affaldspapirprodukt, eller biomassematerialet omfatter en planteremanens, og planteremanensen omfatter eventuelt stængler, blade, avner, avner, majskolber, majsblade, hø, træ, træflis, træpulp og/eller savsmuld, og affaldspapiret eventuelt omfatter bortkastet og brugt fotokopieringspapir, computerprinterpapir, notesbogpapir, notes-blokpapir, skrivemaskinepapir, aviser, magasiner, pap- og papirbaserede emballagematerialer.
16. Fremgangsmåde til at behandle eller modificere en sammensætning omfattende at bringe sammensætningen i kontakt med et polypeptid som anført i krav 5, eller et polypeptid kodet for af en nucleinsyre som anført i krav 1, eller en sammensætning eller fremstillingsprodukt som anført i et vilkårligt af kravene 12 eller 13, eller en enzymgruppering ifølge krav 14, hvor eventuelt sammensætningen er en farmaceutisk sammensætning, en detergentsammensætning, en kontaktlinseopløsning, en affaldssammensætning, et desinficerende middel, et bioforsvarsmiddel eller et biodetoxificeringsmiddel, eller hvor eventuelt sammensætningen er et brændstof, hvor eventuelt sammensætningen er en alkohol, hvor eventuelt alkoholen er ethanol, eller hvor eventuelt sammensætningen er en biomasse eller et biomassemateriale, et papir, papiraffald, genanvendt papirprodukt, papirpulp, papirprodukt, træ, træprodukt, træpulp, tekstil, væv, garn eller fibre, eller hvor eventuelt sammensætningen er en cellulose- eller cel-lulosederivatsammensætning, eller hvor eventuelt sammensætningen er en drikkevare, en fødevare, et foder, et mejeriprodukt eller et ernæringsmæssigt supplement, eller hvor eventuelt fødevaren er en dej, et brød eller et bagt produkt, eller hvor eventuelt drikkevaren, fødevaren, foderstoffet eller det ernæringsmæssige supplement er til dyr, eller hvor eventuelt biomassematerialet omfatter et lignocellulosemateriale stammende fra en landbrugsmæssig afgrøde, eller biomassematerialet er et biprodukt fra en fødevareproduktion eller en foderproduktion eller biomassematerialet er et lignocelluloseaffaldsprodukt eller biomassematerialet er en planteremanens eller et affaldspapir eller affaldspapirprodukt eller biomassematerialet omfatter en planteremanens og planteremanensen omfatter eventuelt stængler, blade, avner, avner, majskolber, majsblade, løv, træ, træflis, træpulp og/eller savsmuld og eventuelt omfatter papiraffaldet bortkastet og brugt fotokopieringspapir, computerprinterpapir, notesbog- papir, notesblokpapir, skrivemaskinepapir, aviser, magasiner, pap- og papirbaserede emballeringsmaterialer.
17. Fremgangsmåde til at hydrolysere, opbryde eller afbryde en arabinoxylan-oligomer omfattende sammensætning omfattende de følgende trin: (a) tilvejebringelse af et polypeptid med en arabinofuranosidaseaktivitet som anført i krav 5 eller et polypeptid kodet for af en nucleinsyre som anført i krav 1, eller en sammensætning eller fremstillingsprodukt som anført i et vilkårligt af kravene 12 eller 13, eller en enzymsamling ifølge krav 14, (b) tilvejebringelse af en sammensætning omfattende en arabinoxylanoli-gomer, og (c) anbringelse af polypeptidet fra trin (a) i kontakt med sammensætningen fra trin (b) ved betingelser, hvor cellulasen hydrolyserer, opbryder eller afbryder den arabinoxylanoligomeromfattende sammensætning.
18. Fremgangsmåde til at fremstille et brændstof omfattende at bringe en sammensætning i kontakt omfattende en cellulose eller en fermenteringsdygtig sukker med et polypeptid som anført i krav 5 eller et polypeptid kodet for af en nucleinsyre som anført i krav 1 eller en sammensætning eller et fremstillet produkt som anført i et vilkårligt af kravene 12 eller 13, eller en enzymgruppe ifølge krav 14, hvor eventuelt sammensætningen omfattende en cellulose eller en fermenteringsdygtig sukker omfatter en plante, planteprodukt eller plantederivat, og planten eller planteproduktet eventuelt omfatter sukkerrørsplanter eller planteprodukter, roer eller sukkerroer, hvede, majs, sojabønner, kartofler, ris eller byg, og eventuelt omfatter brændstoffet en bioethanol eller en blanding af benzin og ethanol.
19. Fremgangsmåde til forarbejdning af et biomassemateriale omfattende at bringe biomassematerialet i kontakt med et polypeptid som anført i krav 5, eller et polypeptid kodet for af en nucleinsyre som anført i krav 1, eller en sammensætning eller et fremstillet produkt som anført i et vilkårligt af kravene 12 eller 13 eller en enzymgruppering ifølge krav 14, hvor biomassematerialet eventuelt stammer fra en landbrugsmæssig afgrøde, er et biprodukt af en fødevare- eller foderproduktion, er et lignocelluloseaffalds-produkt eller er en planteremanens eller et affaldspapir eller et affaldspapirprodukt, og planteremanensen eventuelt omfatter stængler, blade, avner, avner, majskolber, majsblade, hø, træ, træflis, træpulp og savsmuld, og papiraffaldet omfatter bortkastet eller brugt fotokopieringspapir, computerprinterpapir, notes-bogpapir, notesblokpapir, skrivemaskinepapir, aviser, magasiner, pap- og papirbaserede emballeringsmaterialer og eventuelt genererer forarbejdningen af biomassematerialet en bioethanol.
DK11007477T 2006-02-10 2006-12-08 Arabinofuranosidaseenzymer, nucleinsyrer, som koder for dem, og fremgangsmåder til at fremstille og anvende dem DK2420570T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US77278606P 2006-02-10 2006-02-10
EP20060845040 EP1989301B1 (en) 2006-02-10 2006-12-08 Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them

Publications (1)

Publication Number Publication Date
DK2420570T3 true DK2420570T3 (da) 2014-03-10

Family

ID=38371947

Family Applications (5)

Application Number Title Priority Date Filing Date
DK11007476T DK2450439T3 (da) 2006-02-10 2006-12-08 Cellulosenedbrydende enzymer, nucleinsyrer, der koder for dem, og fremgangsmåder til at fremstille og benytte dem
DK06845040T DK1989301T3 (da) 2006-02-10 2006-12-08 Cellulolytiske enzymer, nucleinsyrer, som koder for dem, og fremgangsmåder til at fremstille og anvende dem
DK11007471.3T DK2444487T3 (da) 2006-02-10 2006-12-08 Cellulolytiske enzymer, nukleinsyrer, der koder for dem, samt fremgangsmåder til deres fremstilling og anvendelse
DK11007477T DK2420570T3 (da) 2006-02-10 2006-12-08 Arabinofuranosidaseenzymer, nucleinsyrer, som koder for dem, og fremgangsmåder til at fremstille og anvende dem
DK11007472T DK2415864T3 (da) 2006-02-10 2006-12-08 Oligomerase-2-enzymer (eller beta-xylosidaseenzymer), nucleinsyrer, som koder for dem, og fremgangsmåder til at fremstille og anvende dem

Family Applications Before (3)

Application Number Title Priority Date Filing Date
DK11007476T DK2450439T3 (da) 2006-02-10 2006-12-08 Cellulosenedbrydende enzymer, nucleinsyrer, der koder for dem, og fremgangsmåder til at fremstille og benytte dem
DK06845040T DK1989301T3 (da) 2006-02-10 2006-12-08 Cellulolytiske enzymer, nucleinsyrer, som koder for dem, og fremgangsmåder til at fremstille og anvende dem
DK11007471.3T DK2444487T3 (da) 2006-02-10 2006-12-08 Cellulolytiske enzymer, nukleinsyrer, der koder for dem, samt fremgangsmåder til deres fremstilling og anvendelse

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK11007472T DK2415864T3 (da) 2006-02-10 2006-12-08 Oligomerase-2-enzymer (eller beta-xylosidaseenzymer), nucleinsyrer, som koder for dem, og fremgangsmåder til at fremstille og anvende dem

Country Status (10)

Country Link
US (4) US8101393B2 (da)
EP (9) EP2420570B1 (da)
CN (3) CN103555741A (da)
AU (1) AU2006338208B2 (da)
BR (1) BRPI0621331A2 (da)
CA (1) CA2637013A1 (da)
DK (5) DK2450439T3 (da)
MY (8) MY161027A (da)
NZ (9) NZ595497A (da)
WO (1) WO2007094852A2 (da)

Families Citing this family (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030051836A1 (en) * 2001-05-21 2003-03-20 Novozymes A/S Enzymatic hydrolysis of a polymer comprising vinyl acetate monomer
US7024800B2 (en) 2004-07-19 2006-04-11 Earthrenew, Inc. Process and system for drying and heat treating materials
US7685737B2 (en) 2004-07-19 2010-03-30 Earthrenew, Inc. Process and system for drying and heat treating materials
US7610692B2 (en) 2006-01-18 2009-11-03 Earthrenew, Inc. Systems for prevention of HAP emissions and for efficient drying/dehydration processes
NZ598285A (en) * 2007-01-30 2013-10-25 Syngenta Participations Ag Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them
US7923236B2 (en) * 2007-08-02 2011-04-12 Dyadic International (Usa), Inc. Fungal enzymes
BRPI0817811A2 (pt) * 2007-10-03 2014-10-14 Verenium Corp Xilanases, ácidos nucleicos que codificam as mesmas e métodos para fabricação e uso das mesmas.
CA2707017A1 (en) * 2007-11-30 2009-06-11 Novozymes A/S Polypeptides having arabinofuranosidase activity and polynucleotides encoding same
FR2926824A1 (fr) * 2008-01-25 2009-07-31 Cie Ind De La Matiere Vegetale Procede de pretraitement d'une matiere vegetale lignocellulosique en vue de la production de bioethanol.
CA2724076C (en) 2008-05-11 2018-11-27 Universiteit Stellenbosch Heterologous expression of fungal cellobiohydrolases in yeast
CN101348776B (zh) * 2008-07-31 2011-08-17 中国农业科学院北京畜牧兽医研究所 分泌酸性纤维素酶的转基因乳酸菌及其制备方法和应用
WO2010048522A1 (en) 2008-10-24 2010-04-29 C5-6 Technologies, Inc. Thermostable cellulase and methods of use
WO2010059424A2 (en) 2008-11-18 2010-05-27 Novozymes, Inc. Methods and compositions for degrading cellulosic material
WO2010124266A2 (en) * 2009-04-24 2010-10-28 The Regents Of The University Of California Thermostable cellulases, and mutants thereof, capable of hydrolyzing cellulose in ionic liquid
WO2010129287A2 (en) 2009-04-27 2010-11-11 The Board Of Trustees Of The University Of Illinois Hemicellulose-degrading enzymes
CN101870966B (zh) * 2009-04-27 2012-08-01 复旦大学 葡萄糖苷酶/木糖苷酶双功能纤维素降解酶及其制备方法和应用
MX353237B (es) 2009-09-23 2018-01-08 Danisco Us Inc Enzimas de glicosil hidrolasa novedosas y usos de las mismas.
US9267125B2 (en) * 2009-11-06 2016-02-23 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
US20130071884A1 (en) 2009-11-06 2013-03-21 Agrivida, Inc. Plants expressing cell wall degrading enzymes and expression vectors
RU2012131405A (ru) 2009-12-23 2014-01-27 ДАНИСКО ЮЭс ИНК. Способы улучшения эффективности одновременно протекающих реакций осахаривания и ферментации
FI122937B (fi) 2009-12-30 2012-09-14 Roal Oy Menetelmä selluloosamateriaalin käsittelemiseksi sekä tässä käyttökelpoiset CBH II/Cel6A entsyymit
US9133448B2 (en) * 2010-02-11 2015-09-15 Dsm Ip Assets B.V. Polypeptide having cellobiohydrolase activity and uses thereof
EP2536842A1 (en) 2010-02-15 2012-12-26 Yeda Research and Development Co. Ltd. Bio-engineered multi-enzyme complexes comprising xylanases and uses thereof
CN102918151B (zh) * 2010-03-31 2015-09-09 诺维信股份有限公司 纤维二糖水解酶变体及编码其的多核苷酸
JP5628544B2 (ja) * 2010-04-07 2014-11-19 株式会社豊田中央研究所 クロストリジウム・サーモセラム由来のタンパク質複合体を構築するためのタンパク質及びその利用
ES2371317B1 (es) * 2010-06-14 2012-11-07 Consejo Superior De Investigaciones Científicas (Csic) Endoxilanasa termorresistente obtenida por mutagénesis y su aplicación al proceso de obtención de bioetanol.
CN101906406B (zh) * 2010-06-24 2012-10-17 复旦大学 具有内切葡聚糖酶/木聚糖酶活性的双功能酶及其制备方法与应用
US10443068B2 (en) 2010-06-25 2019-10-15 Agrivida, Inc. Plants with engineered endogenous genes
US9598700B2 (en) 2010-06-25 2017-03-21 Agrivida, Inc. Methods and compositions for processing biomass with elevated levels of starch
DK2735611T3 (da) 2010-08-30 2019-01-28 Novozymes As Polypeptider med cellulolyseforøgende aktivitet og polynukleotider, som koder for dem
US9267126B2 (en) 2010-08-30 2016-02-23 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
US9187742B2 (en) * 2010-08-30 2015-11-17 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
CA2811206A1 (en) * 2010-09-15 2012-03-22 The Regents Of The University Of California Thermophilic mutants of trichoderma reesei endoglucanase i
US9096871B2 (en) * 2010-10-06 2015-08-04 Bp Corporation North America Inc. Variant CBH I polypeptides with reduced product inhibition
KR20130132789A (ko) * 2010-11-05 2013-12-05 아사히 가라스 가부시키가이샤 스키조사카로미세스속 효모의 형질 전환체 및 그 제조 방법
US20140147873A1 (en) * 2011-03-03 2014-05-29 The Regents Of The University Of California Surface display of cellulolytic enzymes and enzyme complexes on gram-positive microorganisms
CA2829207C (en) * 2011-03-07 2022-10-11 Agrivida, Inc. Consolidated pretreatment and hydrolysis of plant biomass expressing cell wall degrading enzymes
CN103492561A (zh) * 2011-03-17 2014-01-01 丹尼斯科美国公司 纤维素酶组合物和使用相同组合物用于改善木质纤维素生物质转化成可发酵糖的方法
SG192025A1 (en) * 2011-03-17 2013-08-30 Danisco Us Inc Glycosyl hydrolase enzymes and uses thereof for biomass hydrolysis
US20140134677A1 (en) * 2011-03-17 2014-05-15 Danisco Us Inc. Method for reducing viscosity in saccharification process
CN103562382A (zh) * 2011-03-25 2014-02-05 诺维信公司 用于降解或转化纤维素材料的方法
CN103608356A (zh) * 2011-04-29 2014-02-26 丹尼斯科美国公司 包含黏琼脂芽孢杆菌(Bacillus agaradhaerens)甘露聚糖酶的洗涤剂组合物及其使用方法
US8835141B2 (en) * 2011-06-09 2014-09-16 The United States Of America As Represented By The Secretary Of Agriculture Methods for integrated conversion of lignocellulosic material to sugars or biofuels and nano-cellulose
WO2013103127A1 (ja) * 2012-01-06 2013-07-11 本田技研工業株式会社 糖化酵素組成物及びそれを用いる糖化溶液の製造方法
EA028648B1 (ru) * 2012-03-30 2017-12-29 Басф Энзаймс Ллк Ген, кодирующий целлюлазу (варианты)
US8980578B2 (en) 2012-06-11 2015-03-17 Codexis, Inc. Fungal beta-xylosidase variants
CN102827819B (zh) * 2012-08-08 2014-03-12 天津工业生物技术研究所 一种β-木糖苷酶及其应用
WO2014070856A2 (en) 2012-11-02 2014-05-08 Bp Corporation North America Inc. Thermotolerant beta-glucosidase variants
CN104870636A (zh) * 2012-12-07 2015-08-26 丹尼斯科美国公司 组合物及使用方法
BR112015012968A2 (pt) * 2012-12-07 2017-09-12 Danisco Us Inc composições e métodos de uso
EP2929023B1 (en) * 2012-12-07 2017-08-16 Danisco US Inc. Compositions and methods of use
BR112015012986A2 (pt) * 2012-12-07 2017-09-12 Danisco Us Inc composições e métodos de uso
US9850512B2 (en) 2013-03-15 2017-12-26 The Research Foundation For The State University Of New York Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield
US9493751B2 (en) * 2013-03-15 2016-11-15 Academia Sinica Dual-functional hybrid glucanases
US20140356913A1 (en) * 2013-03-29 2014-12-04 Matthew Wayne Parrow Enzymes Manufactured in Transgenic Soybean for Plant Biomass Engineering and Organopollutant Bioremediation
HUE047670T2 (hu) * 2013-05-18 2020-05-28 Direvo Ind Biotechnology Gmbh Enzimkészítmények a fermentációs folyamatok és melléktermékek javítására
JP6449272B2 (ja) * 2013-07-08 2019-01-09 ナンジン レジェンド バイオテック カンパニー，リミテッドＮａｎｊｉｎｇ Ｌｅｇｅｎｄ Ｂｉｏｔｅｃｈ Ｃｏ．，Ｌｔｄ． タンパク質の血清半減期を増加する組成物及び方法
US9951363B2 (en) 2014-03-14 2018-04-24 The Research Foundation for the State University of New York College of Environmental Science and Forestry Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects
US10494655B2 (en) 2014-04-03 2019-12-03 Universiti Brunei Darussalam Synthetic fusion gene and its use thereof
GB201409047D0 (en) * 2014-05-21 2014-07-02 Cellucomp Ltd Cellulose microfibrils
US10047351B2 (en) * 2014-05-27 2018-08-14 National Institute Of Advanced Industrial Science And Technology Cellobiohydrolase having improved thermal stability
JP2016029908A (ja) * 2014-07-28 2016-03-07 本田技研工業株式会社 Ｇｈファミリー１０に属する耐熱性キシラナーゼ
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
AR102445A1 (es) * 2014-10-27 2017-03-01 Danisco Us Inc COMPOSICIONES Y MÉTODOS RELACIONADOS CON b-GLUCOSIDASA
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
GB201509149D0 (en) 2015-05-28 2015-07-15 Sintef Thermostatable Cellulases
US20180216089A1 (en) * 2015-07-24 2018-08-02 Novozymes, Inc. Polypeptides Having Beta-Xylosidase Activity And Polynucleotides Encoding Same
WO2017063787A1 (en) * 2015-10-15 2017-04-20 Technische Universitaet Muenchen Mutant beta-glucosidase variants with increased thermostability
CN108603184B (zh) * 2016-02-18 2022-09-16 百奥普拉特公司 阿拉伯聚糖酶及其用途
CN106338453A (zh) * 2016-08-26 2017-01-18 浙江海申新材料有限公司 一种快速检测纤维素醚防霉性能的方法
IL247569A0 (en) 2016-08-30 2016-11-30 Yeda Res & Dev An enzymatic complex for the breakdown of lignocellulosic material
EP3545003A4 (en) * 2016-11-25 2020-12-09 Novozymes A/S GH10 XYLANASE, GH62 ARABINOFURANOSIDASE, CRUSHING PROCESS AND OTHER APPLICATION
WO2018106656A1 (en) 2016-12-06 2018-06-14 Danisco Us Inc Truncated lpmo enzymes and use thereof
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
CA3070606A1 (en) * 2017-07-24 2019-01-31 North Carolina State University Compositions and methods for increasing phytochemical bioavailablity and bioactivity
CN119264211A (zh) 2018-08-27 2025-01-07 瑞泽恩制药公司 拉曼光谱在下游纯化中的应用
CN109182358B (zh) * 2018-09-17 2022-02-08 黑龙江省农业科学院耕作栽培研究所 适用于水稻秸秆还田的芽孢杆菌纤维素酶基因fib1x
CN109504632B (zh) * 2018-12-18 2022-02-25 河北农业大学 一株枯草芽孢杆菌及其应用
CN113496760B (zh) * 2020-04-01 2024-01-12 深圳华大基因科技服务有限公司 基于第三代测序的多倍体基因组组装方法和装置
WO2021248045A2 (en) * 2020-06-04 2021-12-09 California Institute Of Technology Novel signal peptides generated by attention-based neural networks
JP7634960B2 (ja) * 2020-10-28 2025-02-25 花王株式会社 改変シグナルペプチド
EP4052581A1 (en) * 2021-03-03 2022-09-07 AB Enzymes GmbH Use of gh12 cellulases in spelt, oat, barley, and / or millet baking
WO2023114930A1 (en) * 2021-12-15 2023-06-22 The George Washington University Compositions for and methods of improving directed evolution of biomolecules
CN115500382B (zh) * 2021-12-27 2024-07-30 君乐宝乳业集团股份有限公司 一种脱冷控制后酸的乳酸菌饮料及其制备方法
CN114628036B (zh) * 2022-05-17 2022-08-02 中南大学湘雅医院 基于神经网络的脑缺血风险预估平台
DE102023001827A1 (de) 2023-04-19 2024-10-24 Horst Wochnowski Verfahren und dazugehörige Vorrichtung zum Züchten von in Gewässern Mikroplastik zersetzenden und abbauenden Mikroorganismenstämme durch einen künstlich herbeigeführten und beschleunigten Evolutions- und Selektionsprozess
CN117143848B (zh) * 2023-07-17 2025-01-21 大理大学 一种耐盐β-葡萄糖苷酶及应用
CN118308396A (zh) * 2024-05-16 2024-07-09 浙江工业大学 用于遗传转化的重组多粘类芽孢杆菌及其构建方法

Family Cites Families (195)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1138583A (en) 1914-11-09 1915-05-04 Thomas H Tasker Ladder-support.
US5366558A (en) 1979-03-23 1994-11-22 Brink David L Method of treating biomass material
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US5021246A (en) 1984-03-30 1991-06-04 Anheuser-Busch, Incorporated Step mashing process for producing low alcohol beer
US5087571A (en) 1984-06-22 1992-02-11 President And Fellows Of Harvard College Method for providing a cell culture from a transgenic non-human mammal
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DE229046T1 (de) 1985-03-30 1987-12-17 Marc Genf/Geneve Ballivet Verfahren zum erhalten von dns, rns, peptiden, polypeptiden oder proteinen durch dns-rekombinant-verfahren.
US4962028A (en) 1986-07-09 1990-10-09 Dna Plant Technology Corporation Plant promotors
US4946778A (en) 1987-09-21 1990-08-07 Genex Corporation Single polypeptide chain binding molecules
US4987071A (en) 1986-12-03 1991-01-22 University Patents, Inc. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods
US5015580A (en) 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
US5573933A (en) 1987-04-14 1996-11-12 Luminis Pty, Ltd. Transgenic pigs
US4788066A (en) 1987-12-14 1988-11-29 Grain Processing Corporation Preparation of low alcohol beer
US6054270A (en) 1988-05-03 2000-04-25 Oxford Gene Technology Limited Analying polynucleotide sequences
US5700637A (en) 1988-05-03 1997-12-23 Isis Innovation Limited Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays
US5217879A (en) 1989-01-12 1993-06-08 Washington University Infectious Sindbis virus vectors
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5527681A (en) 1989-06-07 1996-06-18 Affymax Technologies N.V. Immobilized molecular synthesis of systematically substituted compounds
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5589583A (en) 1990-01-11 1996-12-31 Monsanto Company Plant promoter
WO1991016427A1 (en) 1990-04-24 1991-10-31 Stratagene Methods for phenotype creation from multiple gene populations
US5326477A (en) 1990-05-07 1994-07-05 Bio-Sep, Inc. Process for digesting solid waste
DK0531372T4 (da) 1990-05-09 2004-08-09 Novozymes As Cellulasepræparat omfattende et endoglucanaseenzym
ES2217250T3 (es) 1990-06-15 2004-11-01 Scios Inc. Mamifero transgenico, no humano que muestra la patologia de formacion amiloides de la enfermedad de alzheimer.
ES2260886T3 (es) 1990-11-23 2006-11-01 Bayer Bioscience N.V. Procedimiento para transformar plantas monocotiledoneas.
ES2322032T3 (es) 1990-12-10 2009-06-16 Genencor Int Sacrificacion mejorada de celulosa por clonacion y amplificacion del gen de beta-glucosidasa de trichoderma reesei.
JP3298879B2 (ja) 1990-12-20 2002-07-08 イグジス，インコーポレイテッド 結合タンパク質の最適化
NL9100050A (nl) 1991-01-11 1992-08-03 Heineken Technische Beheer Bv Werkwijze voor het continu bereiden van wort.
US5405624A (en) 1991-02-14 1995-04-11 Bio-Technical Resources Process for producing a product with an intensified beer flavor
US6110700A (en) 1991-03-11 2000-08-29 The General Hospital Corporation PRAD1 cyclin and its cDNA
US5922854A (en) 1991-06-14 1999-07-13 Kumar; Ramesh Purifying Human Hemogloblin from transgenic pig red cells and plasmids containing pig globin nucleic acids
CA2075135A1 (en) 1991-08-02 1993-02-03 David E. Ellis Particle-mediated transformation of gymnosperms
US5632957A (en) 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
JPH05236997A (ja) 1992-02-28 1993-09-17 Hitachi Ltd ポリヌクレオチド捕捉用チップ
US5550046A (en) 1992-03-27 1996-08-27 Toyo Boseki Kabushiki Kaisha DNA encoding α-glucosidase and method of producing same by genetic engineering
AU5676394A (en) 1992-11-20 1994-06-22 Agracetus, Inc. Transgenic cotton plants producing heterologous bioplastic
BR9307805A (pt) 1992-12-31 1996-07-30 Metallgesellschaft Ag Processo para a preparaçao de cerveja
AU7925094A (en) 1993-09-30 1995-04-18 Agracetus, Inc. Transgenic cotton plants producing heterologous peroxidase
US6045996A (en) 1993-10-26 2000-04-04 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays
US5965452A (en) 1996-07-09 1999-10-12 Nanogen, Inc. Multiplexed active biologic array
US6468742B2 (en) 1993-11-01 2002-10-22 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using bioelectronic microchip
DK131193D0 (da) 1993-11-23 1993-11-23 Novo Nordisk As
US5571703A (en) 1993-12-23 1996-11-05 Controlled Environmental Systems Corporation Municipal solid waste processing facility and commercial ethanol production process
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5639940A (en) 1994-03-03 1997-06-17 Pharmaceutical Proteins Ltd. Production of fibrinogen in transgenic animals
WO1995024494A1 (en) 1994-03-09 1995-09-14 Abbott Laboratories Humanized milk
AU692841B2 (en) 1994-03-09 1998-06-18 Abbott Laboratories Transgenic animals producing oligosaccharides and glycoconjugates
US5824531A (en) 1994-03-29 1998-10-20 Novid Nordisk Alkaline bacilus amylase
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5750870A (en) 1994-06-17 1998-05-12 Agritope, Inc. Plant genetic transformation methods and transgenic plants
US5582681A (en) 1994-06-29 1996-12-10 Kimberly-Clark Corporation Production of soft paper products from old newspaper
US5959171A (en) 1994-08-17 1999-09-28 Pharming B.V. Method for the production of biologically active polypeptides in a mammal's
WO1996006927A1 (en) 1994-09-01 1996-03-07 Merck & Co., Inc. Transgenic animal expressing a familial form of human amyloid precursor protein
US5423145A (en) * 1994-09-16 1995-06-13 Nasset; James L. Rifle-barrel harmonic vibration tuning device
US6111166A (en) 1994-09-19 2000-08-29 Medarex, Incorporated Transgenic mice expressing human Fcα and β receptors
US5795737A (en) 1994-09-19 1998-08-18 The General Hospital Corporation High level expression of proteins
US5880327A (en) 1994-09-21 1999-03-09 American National Red Cross Transgenic mammals expressing human coagulation factor VIII
US5556752A (en) 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
US6187992B1 (en) 1994-12-05 2001-02-13 Merck & Co., Inc. Transgenic mouse having a disrupted amyloid precursor protein gene
US5830645A (en) 1994-12-09 1998-11-03 The Regents Of The University Of California Comparative fluorescence hybridization to nucleic acid arrays
US5633440A (en) 1994-12-20 1997-05-27 Dna Plant Technology Corporation P119 promoters and their uses
US5705369A (en) 1994-12-27 1998-01-06 Midwest Research Institute Prehydrolysis of lignocellulose
CN1165614C (zh) 1995-01-26 2004-09-08 诺沃奇梅兹有限公司 含有木聚糖酶的动物饲料添加剂
US6093562A (en) 1996-02-05 2000-07-25 Novo Nordisk A/S Amylase variants
US5959098A (en) 1996-04-17 1999-09-28 Affymetrix, Inc. Substrate preparation process
AU698739B2 (en) 1995-06-06 1998-11-05 Isis Pharmaceuticals, Inc. Oligonucleotides having phosphorothioate linkages of high chiral purity
CN1188510A (zh) * 1995-06-23 1998-07-22 丹尼斯科成分公司(丹尼斯科公司) 新的β-木糖苷酶和编码该酶的核苷酸序列以及该酶的用途
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
FR2736359B1 (fr) 1995-07-06 1997-10-03 Agronomique Inst Nat Rech Beta-glucosidase de champignons filamenteaux, et ses utilisations
US5985662A (en) 1995-07-13 1999-11-16 Isis Pharmaceuticals Inc. Antisense inhibition of hepatitis B virus replication
US5958672A (en) 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US6057103A (en) 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
EP0839224A1 (en) 1995-07-19 1998-05-06 Novo Nordisk A/S Treatment of fabrics
US5962258A (en) 1995-08-23 1999-10-05 Diversa Corporation Carboxymethyl cellulase fromthermotoga maritima
WO1997016533A1 (en) 1995-10-31 1997-05-09 The Regents Of The University Of California Mammalian artificial chromosomes and methods of using same
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US6358709B1 (en) 1995-12-07 2002-03-19 Diversa Corporation End selection in directed evolution
US6740506B2 (en) 1995-12-07 2004-05-25 Diversa Corporation End selection in directed evolution
US20030215798A1 (en) 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US6361974B1 (en) 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US6939689B2 (en) 1995-12-07 2005-09-06 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6022963A (en) 1995-12-15 2000-02-08 Affymetrix, Inc. Synthesis of oligonucleotide arrays using photocleavable protecting groups
US6013440A (en) 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
US6127160A (en) 1996-03-14 2000-10-03 Japan As Represented By Director General Of Agency Of Industrial Science And Technology Protein having cellulase activities and process for producing the same
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US6025155A (en) 1996-04-10 2000-02-15 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US5697987A (en) 1996-05-10 1997-12-16 The Trustees Of Princeton University Alternative fuel
US6197070B1 (en) 1996-05-15 2001-03-06 The Procter & Gamble Company Detergent compositions comprising alpha combination of α-amylases for malodor stripping
US6057100A (en) 1996-06-07 2000-05-02 Eos Biotechnology, Inc. Oligonucleotide arrays
US5833857A (en) 1996-06-07 1998-11-10 Lytal Family Trust Mobile Bioreactor and Biogenerator
US5747320A (en) 1996-08-02 1998-05-05 The United States Of America, As Represented By The Secretary Of Agriculture Glucose and cellobiose tolerant β-glucosidase from Candida peltata
AU4067297A (en) 1996-08-16 1998-03-06 International Paper Company Enzymatic freeness enhancement
US6326341B1 (en) 1996-09-11 2001-12-04 The Procter & Gamble Company Low foaming automatic dishwashing compositions
US6069122A (en) 1997-06-16 2000-05-30 The Procter & Gamble Company Dishwashing detergent compositions containing organic diamines for improved grease cleaning, sudsing, low temperature stability and dissolution
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
KR100570935B1 (ko) 1997-01-17 2006-04-13 맥시겐, 인크. 반복적 서열 재조합에 의한 전세포 및 유기체의 개량 방법
DE19703364A1 (de) 1997-01-30 1998-08-06 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Wasch- und Reinigungsmittel
ATE418608T1 (de) 1997-03-18 2009-01-15 Novozymes As Methode zur herstellung einer bibliothek mittels dna-shuffling
BR9808404A (pt) 1997-03-18 2000-05-16 2B Ag Processo para aproveitamento de biomassa vegetal e prensa helicoidal para realização desse processo
WO1998041653A1 (en) 1997-03-18 1998-09-24 Novo Nordisk A/S An in vitro method for construction of a dna library
US5948653A (en) 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
US6153410A (en) 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
US6333181B1 (en) 1997-04-07 2001-12-25 University Of Florida Research Foundation, Inc. Ethanol production from lignocellulose
RU2127760C1 (ru) 1997-04-22 1999-03-20 Акционерное общество открытого типа "Туласпирт" спиртоводочный завод "Лужковский" Способ производства этилового спирта из зернового сырья
US5916780A (en) 1997-06-09 1999-06-29 Iogen Corporation Pretreatment process for conversion of cellulose to fuel ethanol
US7019827B2 (en) 1997-06-16 2006-03-28 Diversa Corporation GigaMatrix holding tray having through-hole wells
AUPO758297A0 (en) 1997-06-27 1997-07-24 Rowe, James Baber Control of acidic gut syndrome
NZ328434A (en) 1997-07-24 1998-05-27 Univ British Columbia Substitu Coniferin beta-glucosidase cdna for modifying lignin content in plants
US6826296B2 (en) 1997-07-25 2004-11-30 Affymetrix, Inc. Method and system for providing a probe array chip design database
US5958758A (en) 1997-08-04 1999-09-28 Biosun Systems Corporation Treatment of animal waste
EP1012335A4 (en) 1997-08-15 2004-06-09 Hyseq Inc EXPERIENCE AND COMPOSITIONS FOR DETECTING OR QUANTIFYING NUCLEIC ACID SPECIES
IL134702A (en) 1997-09-11 2004-03-28 Bioventures Inc High density arrays and methods of making the same
US6465178B2 (en) 1997-09-30 2002-10-15 Surmodics, Inc. Target molecule attachment to surfaces
US6399383B1 (en) 1997-10-28 2002-06-04 Maxygen, Inc. Human papilloma virus vectors
US6204232B1 (en) 1997-10-30 2001-03-20 Novo Nordisk A/S α-amlase mutants
EP1030861A4 (en) 1997-10-31 2001-09-05 Maxygen Inc MODIFICATION OF VIRUSTROPISMIUS AND THE ECONOMIC SPECTRUM BY VIRAL GENOM MIXING
EP1032644B1 (en) 1997-11-10 2003-03-19 The Procter & Gamble Company Process for preparing a detergent tablet
DE69828574T2 (de) 1997-11-14 2005-10-06 Sankyo Co., Ltd. Transgenes Tier als Modell für Allergie und seine Verwendung
US6537746B2 (en) 1997-12-08 2003-03-25 Maxygen, Inc. Method for creating polynucleotide and polypeptide sequences
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
EP1054973A1 (en) 1998-02-11 2000-11-29 Maxygen, Inc. Antigen library immunization
AU3291099A (en) 1998-02-11 1999-08-30 Maxygen, Inc. Genetic vaccine vector engineering
CA2323638A1 (en) 1998-04-03 1999-10-14 Phylos, Inc. Addressable protein arrays
US6156952A (en) 1998-04-09 2000-12-05 Constituent Institution Of The University Of Maryland System HIV transgenic animals and uses therefor
JP2003527450A (ja) 1998-05-01 2003-09-16 ノボ ノルディスク アクティーゼルスカブ Ｎ−ヒドロキシアセトアニリドの様な増強剤
US6048695A (en) 1998-05-04 2000-04-11 Baylor College Of Medicine Chemically modified nucleic acids and methods for coupling nucleic acids to solid support
EP0976838A1 (en) 1998-05-06 2000-02-02 Rhone-Poulenc Nutrition Animale Enzymes mixture
WO1999057287A2 (en) 1998-05-06 1999-11-11 University Of Georgia Research Foundation, Inc. Beta-glucosidase coding sequences and protein from orpinomyces pc-2
DE19824705A1 (de) 1998-06-03 1999-12-09 Henkel Kgaa Amylase und Protease enthaltende Wasch- und Reinigungsmittel
IL140100A0 (en) 1998-06-29 2002-02-10 Phylos Inc Methods of generating highly diverse libraries
JP4335995B2 (ja) 1998-07-22 2009-09-30 昭 神谷 環境保全型粒状洗浄用組成物
FR2782323B1 (fr) 1998-08-12 2002-01-11 Proteus Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues
GB2340755B (en) 1998-08-24 2002-09-25 Cannon Rubber Ltd Breast pump insert
US6602700B1 (en) 1998-09-04 2003-08-05 University Of Georgia Research Foundation, Inc. Phenolic acid esterases, coding sequences and methods
AU6046199A (en) 1998-09-17 2000-04-03 Novozymes North America, Inc. Methods for deinking and decolorizing printed paper
US6277628B1 (en) 1998-10-02 2001-08-21 Incyte Genomics, Inc. Linear microarrays
EP1123974B1 (en) 1998-10-23 2010-05-05 Meiji Seika Kaisha Ltd. Endoglucanases and cellulase preparations containing the same
US6277489B1 (en) 1998-12-04 2001-08-21 The Regents Of The University Of California Support for high performance affinity chromatography and other uses
US7220542B2 (en) 2000-07-17 2007-05-22 Van Den Brink Johannes Maarten Expression cloning in filamentous fungi
AU1685600A (en) 1998-12-24 2000-07-31 Takara Shuzo Co., Ltd. Polypeptides
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6511824B1 (en) 1999-03-17 2003-01-28 Exelixis, Inc. Nucleic acids and polypeptides of invertebrate TWIK channels and methods of use
US6309871B1 (en) 1999-03-31 2001-10-30 Novozymes A/S Polypeptides having alkaline α-amylase activity
US6221653B1 (en) 1999-04-27 2001-04-24 Agilent Technologies, Inc. Method of performing array-based hybridization assays using thermal inkjet deposition of sample fluids
US6653151B2 (en) 1999-07-30 2003-11-25 Large Scale Proteomics Corporation Dry deposition of materials for microarrays using matrix displacement
US6409841B1 (en) 1999-11-02 2002-06-25 Waste Energy Integrated Systems, Llc. Process for the production of organic products from diverse biomass sources
CA2360750A1 (en) 1999-11-22 2001-05-31 Diversa Corporation Capillary array-based sample screening
US20010008765A1 (en) 1999-12-06 2001-07-19 Fuji Photo Film Co., Ltd. DNA chip and reactive solid carrier
US6531644B1 (en) 2000-01-14 2003-03-11 Exelixis, Inc. Methods for identifying anti-cancer drug targets
WO2001070998A1 (en) 2000-03-20 2001-09-27 Dsm N.V. Talaromyces emersonii beta-glucanases
AU2001266978A1 (en) 2000-06-14 2001-12-24 Diversa Corporation Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating
US6423145B1 (en) 2000-08-09 2002-07-23 Midwest Research Institute Dilute acid/metal salt hydrolysis of lignocellulosics
CA2422558C (en) 2000-09-25 2012-02-14 Iogen Energy Corporation Method for glucose production with a modified cellulase mixture
US7256031B2 (en) * 2000-09-29 2007-08-14 Meiji Seika Kaisha, Ltd. Enzyme having β-glucosidase activity and use thereof
WO2002029032A2 (en) 2000-09-30 2002-04-11 Diversa Corporation Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating
JP2005501217A (ja) 2000-10-10 2005-01-13 ディベルサ コーポレーション 生体活性または生体分子のハイスループットスクリーニングまたはキャピラリーに基づくスクリーニング
EP1332153A4 (en) 2000-10-12 2006-01-11 Exelixis Inc HUMAN ECT2 AND METHOD OF USE THEREOF
US6309872B1 (en) 2000-11-01 2001-10-30 Novozymes Biotech, Inc Polypeptides having glucoamylase activity and nucleic acids encoding same
EP1347770A4 (en) 2000-11-30 2005-10-19 Diversa Corp PROCESS FOR PREPARING A PROTEIN POLYMER AND USE OF THE POLYMER
ES2521615T3 (es) 2001-06-06 2014-11-13 Novozymes A/S Endo-beta-1,4-glucanasa
DK2295544T3 (da) 2001-06-26 2016-09-26 Novozymes As Polypeptider med cellobiohydrolase I-aktivitet og polynukleotider, der koder for samme
US7393673B2 (en) 2001-07-28 2008-07-01 Midwest Research Institute Thermal tolerant exoglucanase from Acidothermus cellulolyticus
WO2003012109A1 (en) 2001-07-28 2003-02-13 Midwest Research Institute Thermal tolerant cellulase from acidothermus cellulolyticus
US6979733B2 (en) * 2001-08-03 2005-12-27 Diversa Corporation Epoxide hydrolases, nucleic acids encoding them and methods of making and using them
EP1446495A4 (en) 2001-10-01 2006-06-07 Diversa Corp ENTIRE CELL ENGINEERING USING REAL-TIME METABOLIC FLOW ANALYSIS
US7314974B2 (en) 2002-02-21 2008-01-01 Monsanto Technology, Llc Expression of microbial proteins in plants for production of plants with improved properties
US6918738B2 (en) 2002-03-11 2005-07-19 Diversa Corporation Stackable sample holding plate with robot removable lid
US20040005674A1 (en) 2002-04-30 2004-01-08 Athenix Corporation Methods for enzymatic hydrolysis of lignocellulose
USD480814S1 (en) 2002-06-11 2003-10-14 Diversa Corporation Gigamatrix holding tray
DK2305801T3 (da) 2002-08-16 2017-10-09 Danisco Us Inc Hidtil ukendte hypocrea jecorina-cbh1-cellulaser
US6776979B2 (en) 2002-12-30 2004-08-17 Marvin B. Frager Periodontal treatment compound and method of use
WO2004078919A2 (en) * 2003-02-27 2004-09-16 Midwest Research Institute Superactive cellulase formulation using cellobiohydrolase-1 from penicillium funiculosum
US20040231060A1 (en) 2003-03-07 2004-11-25 Athenix Corporation Methods to enhance the activity of lignocellulose-degrading enzymes
US7960148B2 (en) 2003-07-02 2011-06-14 Verenium Corporation Glucanases, nucleic acids encoding them and methods for making and using them
EP1687419B1 (en) 2003-10-28 2010-02-03 Novozymes North America, Inc. Hybrid enzymes
US7338763B2 (en) 2004-06-02 2008-03-04 Eppendorf Array Technologies S.A. Method and kit for the detection and/or quantification of homologous nucleotide sequences on arrays
US20060147581A1 (en) 2004-12-22 2006-07-06 Novozymes A/S Hybrid enzymes
WO2006096527A2 (en) * 2005-03-04 2006-09-14 Diversa Corporation Nucleic acids and proteins and methods for making and using them
CA2611859C (en) 2005-03-15 2015-03-31 Verenium Corporation Cellulases, nucleic acids encoding them and methods for making and using them
AU2007244753B2 (en) * 2006-04-28 2012-05-17 C5-6 Technologies, Inc. Thermostable cellulase and methods of use
NZ598285A (en) * 2007-01-30 2013-10-25 Syngenta Participations Ag Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them
JP6449266B2 (ja) 2013-06-21 2019-01-09 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド 流体ピックアップを備えるマイクロ流体システム
KR102193685B1 (ko) 2014-05-02 2020-12-21 삼성전자주식회사 수직 구조의 비휘발성 메모리 소자

Also Published As

Publication number Publication date
EP1989301A4 (en) 2009-08-12
MY160770A (en) 2017-03-15
CA2637013A1 (en) 2007-08-23
NZ595502A (en) 2013-11-29
EP2447363B1 (en) 2014-02-12
EP2444489A1 (en) 2012-04-25
EP2450439B1 (en) 2013-11-27
NZ595500A (en) 2013-05-31
EP2450439A1 (en) 2012-05-09
NZ595495A (en) 2013-05-31
US20160108386A1 (en) 2016-04-21
CN103602692A (zh) 2014-02-26
CN103555741A (zh) 2014-02-05
US20120266328A1 (en) 2012-10-18
EP2447363A1 (en) 2012-05-02
EP1989301A2 (en) 2008-11-12
NZ595499A (en) 2013-05-31
WO2007094852A3 (en) 2008-06-05
DK2444487T3 (da) 2018-05-28
EP2444488B1 (en) 2014-02-12
EP2444488A1 (en) 2012-04-25
DK2415864T3 (da) 2014-05-05
NZ595496A (en) 2013-05-31
MY160756A (en) 2017-03-15
BRPI0621331A2 (pt) 2011-12-20
MY162321A (en) 2017-05-31
EP2420570B1 (en) 2013-12-04
EP2444487B1 (en) 2018-04-18
US9175275B2 (en) 2015-11-03
EP1989301B1 (en) 2014-09-24
WO2007094852A2 (en) 2007-08-23
AU2006338208A1 (en) 2007-08-23
EP2444490A1 (en) 2012-04-25
NZ595498A (en) 2013-05-31
EP2415864A1 (en) 2012-02-08
MY147955A (en) 2013-02-15
US8101393B2 (en) 2012-01-24
MY160710A (en) 2017-03-15
NZ571087A (en) 2012-04-27
NZ595501A (en) 2013-05-31
EP2420570A1 (en) 2012-02-22
CN101426909A (zh) 2009-05-06
DK2450439T3 (da) 2014-02-10
EP2444489B1 (en) 2014-02-12
MY161026A (en) 2017-03-31
WO2007094852A8 (en) 2008-11-13
AU2006338208B2 (en) 2013-10-24
DK1989301T3 (da) 2015-01-05
US20130227741A1 (en) 2013-08-29
MY161027A (en) 2017-03-31
EP2444490B1 (en) 2014-02-12
EP2444487A1 (en) 2012-04-25
EP2415864B1 (en) 2014-02-12
MY160772A (en) 2017-03-15
NZ595497A (en) 2013-09-27
US20090220480A1 (en) 2009-09-03
US9127263B2 (en) 2015-09-08

Similar Documents

Publication Publication Date Title
DK2420570T3 (da) Arabinofuranosidaseenzymer, nucleinsyrer, som koder for dem, og fremgangsmåder til at fremstille og anvende dem
US8426184B2 (en) Cellulases, nucleic acids encoding them and methods for making and using them
AU2013205502B2 (en) Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them