DK2295544T3 - Polypeptider med cellobiohydrolase I-aktivitet og polynukleotider, der koder for samme - Google Patents

Polypeptider med cellobiohydrolase I-aktivitet og polynukleotider, der koder for samme Download PDF

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DK2295544T3
DK2295544T3 DK10179594.6T DK10179594T DK2295544T3 DK 2295544 T3 DK2295544 T3 DK 2295544T3 DK 10179594 T DK10179594 T DK 10179594T DK 2295544 T3 DK2295544 T3 DK 2295544T3
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ser
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asp
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DK10179594.6T
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Dominique Aubert
Lene Lange
Wenping Wu
Sara Landvik
Kirk Matthew Schnorr
Ib Groth Clausen
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Novozymes As
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38645—Preparations containing enzymes, e.g. protease or amylase containing cellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/14—Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)

Claims (13)

1. Polypeptid med cellobiohydrolase l-aktivitet, der er udvalgt fra gruppen, som består af: (a) et polypeptid, der omfatter en aminosyresekvens, hvilken aminosyresekvens har mindst 80 % identitet med aminosyrerne 1 til 532 af SEQ ID NO: 56, (b) et polypeptid, der omfatter en aminosyresekvens, hvilken aminosyresekvens har mindst 80 % identitet med polypeptidet, der kodes af nu-kleotiderne 1 til 1599 af SEQ ID NO: 55.
2. Polypeptid ifølge krav 1, der omfatter en aminosyresekvens, som har mindst 85 % identitet, fortrinsvis mindst 90 % identitet, mere fortrinsvis mindst 95 % identitet, med aminosyrerne 1 til 532 af SEQ ID NO: 56.
3. Polypeptid ifølge et hvilket som helst af kravene 1-2, hvilket polypeptid består af aminosyrerne 1 til 532 af SEQ ID NO: 56.
4. Polynukleotid, der har en nukleotidsekvens, der koder for polypeptidet som defineret i et hvilket som helst af kravene 1-3.
5. Nukleinsyrekonstruktion, der omfatter nukleotidsekvensen som defineret i krav 4, der er ope-rabelt forbundet til en eller flere kontrolsekvenser, der kontrollerer produktionen af polypeptidet i en egnet vært.
6. Rekombinant ekspressionsvektor, der omfatter nukleinsyrekonstruktionen som defineret i krav 5.
7. Rekombinant værtscelle, der omfatter nukleinsyrekonstruktionen som defineret i krav 5.
8. Fremgangsmåde til produktion af et polypeptid som defineret i et hvilket som helst af kravene 1-3, hvilken fremgangsmåde omfatter: (a) dyrkning af en rekombinant værtscelle som defineret i krav 7 under betingelser, der bidrager til produktion af polypeptidet; og (b) udvinding af polypeptidet.
9. Fremgangsmåde til in situ-produktion af et polypeptid som defineret i et hvilket som helst af kravene 1 -3, hvilken fremgangsmåde omfatter: (a) dyrkning af en rekombinant værtscelle som defineret i krav 7 under betingelser, der bidrager til produktion af polypeptidet; og (b) kontaktbringning af polypeptidet med et ønsket substrat uden forudgående udvinding af polypeptidet.
10. Fremgangsmåde til fremstilling af ethanol fra biomasse, der omfatter kontaktbringning af biomassen med polypeptidet som defineret i et hvilket som helst af kravene 1-3.
11. Anvendelse af polypeptidet som defineret i et hvilket som helst af kravene 1-3 til fremstilling af ethanol.
12. Transgen plante, plantedel eller plantecelle, der omfatter en nukleotidsekvens, der koder for et polypeptid med cellobiohydrolase l-aktivitet som defineret i et hvilket som helst af kravene 1-3.
13. Detergentsammensætning, der omfatter et overfladeaktivt middel og polypeptidet ifølge et hvilket som helst af kravene 1-3.
DK10179594.6T 2001-06-26 2002-06-26 Polypeptider med cellobiohydrolase I-aktivitet og polynukleotider, der koder for samme DK2295544T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200101000 2001-06-26
EP02745180A EP1421224B1 (en) 2001-06-26 2002-06-26 Polypeptides having cellobiohydrolase i activity and polynucleotides encoding same

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DK2295544T3 true DK2295544T3 (da) 2016-09-26

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DK10179594.6T DK2295544T3 (da) 2001-06-26 2002-06-26 Polypeptider med cellobiohydrolase I-aktivitet og polynukleotider, der koder for samme
DK10179578.9T DK2277997T3 (da) 2001-06-26 2002-06-26 Polypeptider med cellobiohydrolase I-aktivitet og polynukleotider, der koder for samme
DK10179521.9T DK2298868T3 (da) 2001-06-26 2002-06-26 Polypeptider med cellobiohydrolase I-aktivitet og polynukleotider, der koder for samme
DK02745180.6T DK1421224T3 (da) 2001-06-26 2002-06-26 Polypeptider med cellobiohydrolase I aktivitet og polynukleotider der koder for samme

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DK10179578.9T DK2277997T3 (da) 2001-06-26 2002-06-26 Polypeptider med cellobiohydrolase I-aktivitet og polynukleotider, der koder for samme
DK10179521.9T DK2298868T3 (da) 2001-06-26 2002-06-26 Polypeptider med cellobiohydrolase I-aktivitet og polynukleotider, der koder for samme
DK02745180.6T DK1421224T3 (da) 2001-06-26 2002-06-26 Polypeptider med cellobiohydrolase I aktivitet og polynukleotider der koder for samme

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US (10) US7785853B2 (da)
EP (4) EP2277997B1 (da)
CN (1) CN1620501A (da)
AU (1) AU2002316809A1 (da)
DK (4) DK2295544T3 (da)
ES (4) ES2589831T3 (da)
WO (1) WO2003000941A2 (da)

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