DK2812430T3 - Pentose-fermenterende mikroorganismer - Google Patents

Pentose-fermenterende mikroorganismer Download PDF

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DK2812430T3
DK2812430T3 DK13706186.7T DK13706186T DK2812430T3 DK 2812430 T3 DK2812430 T3 DK 2812430T3 DK 13706186 T DK13706186 T DK 13706186T DK 2812430 T3 DK2812430 T3 DK 2812430T3
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DK13706186.7T
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Zdravko Dragovic
Christian Gamauf
Christoph Reisinger
Ulrich Kettling
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Clariant Produkte Deutschland Gmbh
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    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N9/90—Isomerases (5.)
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    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
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    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
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    • C12P7/00—Preparation of oxygen-containing organic compounds
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    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
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    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01005—Xylose isomerase (5.3.1.5)
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    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
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  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (12)

1. Gærcelle, der eksprimerer et protein omfattende en aminosyresekvens med mindst 80 % identitet, mest foretrukket 90 % identitet, allermest foretrukket 95 % identitet med SEQ ID NO. 2 og med xylose-isomerase-aktivitet i en gærcelle.
2. Gærcelle ifølge krav 1, hvor proteinet består af sekvensen ifølge SEQ ID NO. 2 og har xylose-isomerase-aktivitet i gærcellen.
3. Gærcelle ifølge krav 1 eller 2, hvor proteinet består af sekvensen ifølge SEQ ID NO. 8, eller af an aminosyresekvens med mindst 75 % identitet, foretrukket 80 % identitet, mest foretrukket 90 % identitet, allermest foretrukket 95 % identitet med SEQ SEQ ID NO. 8 og har xylose-isomerase-aktivitet i cellen.
4. Gærcelle ifølge et af kravene 1 til 3, hvor proteinet har optimal xylose-isomerase-aktivitet inden for et pH-område på 7,5 til 8,5.
5. Gærcelle ifølge et af kravene 1 til 4 omfattende et DNA-molekyle omfattende en DNA-sekvens, der koder et protein som defineret i et af kravene 1 til 4, hvor DNA-sekvensen er operabelt forbundet med en eukaryotisk regulato-risk sekvens.
6. Gærcelle ifølge krav 5, hvor the DNA-molekyldet består af sekvensen ifølge SEQ ID NO. 1 eller SEQ SEQ ID NO. 7.
7. Gærcelle ifølge et af kravene 1 til 6, hvor gærcellen er udvalgt fra gruppen af Pichia, Pachysolen, Yarrowia, Saccharomyces, Candida, Arxula, Ashbya, Debaryomyces, Hansenula, Hartaea, Kluyveromyces, Schwanniomyces, Trichosporon, Xanthophylomyces, Schizosaccharomyces, Zygosaccharomy-ces, mest foretrukket Saccharomyces cerevisiae.
8. Gærcelle ifølge et af kravene 1 til 7 omfattende et eksogent xylose-iso-merase-gen, der er funktionsdygtigt i gærcellen, hvor det eksogene xylose-isomerase-gen er operabelt forbundet med promotor- og terminator-sekven- ser, som er funktionsdygtige i gærcellen, hvilket fører til ekspressionen af et protein som defineret i et af kravene 1 til 4.
9. Gærcelle ifølge krav 8 hvor det eksogene xylose-isomerase-gen er et DNA-molekyle som defineret i krav 5 eller 6.
10. Anvendelse af gærcellen ifølge et af kravene 1 til 9 til fermentering af biomasse ud fra et medium, der indeholder en xylose-carbon-kilde.
11. Anvendelse af gærcellen ifølge et af kravene 1 til 9 som en biokatalysator in situ eller i oprenset form til fremstilling af isomeriserede sukkerprodukter, foretrukket til isomeriserede sukkerprodukter.
12. Fremgangsmåde til fremstilling af et fermenteringsprodukt udvalgt fra gruppen af mælkesyre, eddikesyre, ravsyre, aminosyrer, 1,3-propan-diol, ethylen, glycerol, β-lactam-antibiotika, cephalosporiner, biobrændstoffer, butanol, ethanol, mælkesyre, itakonsyre, foretrukket butanol, mest foretrukket ethanol, hvor fremgangsmåden omfatter de følgende trin: a) fermentering af et medium, der indeholder en kilde af xylose, med en gærcelle som defineret i et af kravene 1 til 9, og eventuelt, b) udvinding af fermenteringsproduktet.
DK13706186.7T 2012-02-07 2013-02-07 Pentose-fermenterende mikroorganismer DK2812430T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP12000783.6A EP2626423A1 (en) 2012-02-07 2012-02-07 Pentose-fermenting microorganism
PCT/EP2013/052407 WO2013117631A1 (en) 2012-02-07 2013-02-07 Pentose fermenting microorganisms

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DK2812430T3 true DK2812430T3 (da) 2017-10-23

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US (2) US20140377813A1 (da)
EP (2) EP2626423A1 (da)
CN (1) CN104204202B (da)
AU (1) AU2013218001B2 (da)
BR (1) BR112014019578B1 (da)
CA (1) CA2863734C (da)
DK (1) DK2812430T3 (da)
EA (1) EA035000B1 (da)
ES (1) ES2643046T3 (da)
HR (1) HRP20171583T1 (da)
HU (1) HUE035703T2 (da)
MX (1) MX350005B (da)
MY (1) MY171359A (da)
NO (1) NO2812430T3 (da)
PL (1) PL2812430T3 (da)
RS (1) RS56569B1 (da)
SI (1) SI2812430T1 (da)
WO (1) WO2013117631A1 (da)
ZA (1) ZA201405761B (da)

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Publication number Priority date Publication date Assignee Title
US9187743B2 (en) 2013-03-11 2015-11-17 E I Du Pont De Nemours And Company Bacterial xylose isomerases active in yeast cells
CA2991707C (en) 2015-07-13 2023-08-01 MARA Renewables Corporation Enhancing microbial metabolism of c5 organic carbon
BR112018002284A2 (pt) 2015-08-05 2018-12-11 Cargill Inc método de fermentação para produzir um bioproduto, levedura crabtree negativa geneticamente engenheirada e método para reduzir a produção de glicerol por uma levedura engenheirada
EP3559210A1 (en) * 2016-12-21 2019-10-30 Vib Vzw Xylose isomerases that confer efficient xylose fermentation capability to yeast
KR20200135469A (ko) * 2018-03-27 2020-12-02 바스프 에스이 자일로스 대사 효모

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DE60325457D1 (de) * 2002-01-23 2009-02-05 Royal Nedalco B V Fermentation von pentosezuckern
DE102008031350B4 (de) * 2008-07-02 2011-02-10 Johann Wolfgang Goethe-Universität Frankfurt am Main Prokaryotische Xylose-Isomerase zur Konstruktion Xylose-vergärender Hefen
CN102791858B (zh) * 2009-12-22 2014-11-26 株式会社丰田中央研究所 木糖异构酶及其利用
WO2012009272A2 (en) * 2010-07-14 2012-01-19 Codexis, Inc. Pentose fermentation by a recombinant microorganism

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US20140377813A1 (en) 2014-12-25
US20160312177A1 (en) 2016-10-27
EA035000B1 (ru) 2020-04-16
CA2863734A1 (en) 2013-08-15
EP2626423A1 (en) 2013-08-14
AU2013218001A1 (en) 2014-08-28
EP2812430A1 (en) 2014-12-17
CN104204202B (zh) 2017-08-01
HRP20171583T1 (hr) 2017-12-01
MY171359A (en) 2019-10-10
BR112014019578A8 (pt) 2017-07-11
MX2014009496A (es) 2014-09-11
PL2812430T3 (pl) 2018-02-28
SI2812430T1 (en) 2018-03-30
NO2812430T3 (da) 2018-02-03
BR112014019578B1 (pt) 2022-10-11
EP2812430B1 (en) 2017-09-06
ZA201405761B (en) 2015-11-25
ES2643046T3 (es) 2017-11-21
BR112014019578A2 (en) 2019-10-15
HUE035703T2 (en) 2018-05-28
WO2013117631A1 (en) 2013-08-15
MX350005B (es) 2017-08-22
CA2863734C (en) 2020-03-24
CN104204202A (zh) 2014-12-10
RS56569B1 (sr) 2018-02-28
EA201400877A1 (ru) 2015-01-30
AU2013218001B2 (en) 2016-02-18

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