EA201400877A1 - Микроорганизмы, ферментирующие пентозы - Google Patents

Микроорганизмы, ферментирующие пентозы

Info

Publication number
EA201400877A1
EA201400877A1 EA201400877A EA201400877A EA201400877A1 EA 201400877 A1 EA201400877 A1 EA 201400877A1 EA 201400877 A EA201400877 A EA 201400877A EA 201400877 A EA201400877 A EA 201400877A EA 201400877 A1 EA201400877 A1 EA 201400877A1
Authority
EA
Eurasian Patent Office
Prior art keywords
identity
eukaryotic cell
microorganisms
sugars
seq
Prior art date
Application number
EA201400877A
Other languages
English (en)
Other versions
EA035000B1 (ru
Inventor
Здравко ДРАГОВИЧ
Кристиан Гамауф
Кристоф Райзингер
Ульрих Кетлинг
Original Assignee
Клариант Продукте (Дойчланд) Гмбх
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Клариант Продукте (Дойчланд) Гмбх filed Critical Клариант Продукте (Дойчланд) Гмбх
Publication of EA201400877A1 publication Critical patent/EA201400877A1/ru
Publication of EA035000B1 publication Critical patent/EA035000B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C12P7/20—Glycerol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/54—Acetic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01005—Xylose isomerase (5.3.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Изобретение обеспечивает микробную эукариотическую клетку, способную к утилизации С5 сахаров, в частности ксилозы. Другой задачей изобретения является обеспечение усовершенствованной белковой последовательности для обеспечения деградации С5 сахаров эукариотической клеткой. Таким образом, настоящее изобретение обеспечивает белок, содержащий аминокислотную последовательность, обладающую по меньшей мере 75% идентичностью, предпочтительно 80% идентичностью, более предпочтительно 90% идентичностью, наиболее предпочтительно 95% идентичностью с SEQ ID № 2 или SEQ ID № 8, и обладающий ксилозоизомеразной активностью в эукариотической клетке.
EA201400877A 2012-02-07 2013-02-07 Эукариотическая клетка, содержащая белок с ксилозоизомеразной активностью, и ее применение EA035000B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP12000783.6A EP2626423A1 (en) 2012-02-07 2012-02-07 Pentose-fermenting microorganism
PCT/EP2013/052407 WO2013117631A1 (en) 2012-02-07 2013-02-07 Pentose fermenting microorganisms

Publications (2)

Publication Number Publication Date
EA201400877A1 true EA201400877A1 (ru) 2015-01-30
EA035000B1 EA035000B1 (ru) 2020-04-16

Family

ID=47750621

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201400877A EA035000B1 (ru) 2012-02-07 2013-02-07 Эукариотическая клетка, содержащая белок с ксилозоизомеразной активностью, и ее применение

Country Status (19)

Country Link
US (2) US20140377813A1 (ru)
EP (2) EP2626423A1 (ru)
CN (1) CN104204202B (ru)
AU (1) AU2013218001B2 (ru)
BR (1) BR112014019578B1 (ru)
CA (1) CA2863734C (ru)
DK (1) DK2812430T3 (ru)
EA (1) EA035000B1 (ru)
ES (1) ES2643046T3 (ru)
HR (1) HRP20171583T1 (ru)
HU (1) HUE035703T2 (ru)
MX (1) MX350005B (ru)
MY (1) MY171359A (ru)
NO (1) NO2812430T3 (ru)
PL (1) PL2812430T3 (ru)
RS (1) RS56569B1 (ru)
SI (1) SI2812430T1 (ru)
WO (1) WO2013117631A1 (ru)
ZA (1) ZA201405761B (ru)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9187743B2 (en) 2013-03-11 2015-11-17 E I Du Pont De Nemours And Company Bacterial xylose isomerases active in yeast cells
EP3322796A4 (en) 2015-07-13 2019-03-27 Mara Renewables Corporation IMPROVEMENT OF MICROGENONAL FLUID CHANGES OF XYLOSE
WO2017024150A1 (en) 2015-08-05 2017-02-09 Cargill, Incorporated Xylose isomerase-modified yeast strains and methods for bioproduct production
EP3559210A1 (en) * 2016-12-21 2019-10-30 Vib Vzw Xylose isomerases that confer efficient xylose fermentation capability to yeast
WO2019185737A1 (en) * 2018-03-27 2019-10-03 Basf Se Xylose metabolizing yeast

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2319757T5 (es) * 2002-01-23 2018-05-22 Dsm Ip Assets B.V. Fermentación de azúcares de pentosa
DE102008031350B4 (de) * 2008-07-02 2011-02-10 Johann Wolfgang Goethe-Universität Frankfurt am Main Prokaryotische Xylose-Isomerase zur Konstruktion Xylose-vergärender Hefen
WO2011078262A1 (ja) * 2009-12-22 2011-06-30 株式会社豊田中央研究所 キシロースイソメラーゼ及びその利用
WO2012009272A2 (en) * 2010-07-14 2012-01-19 Codexis, Inc. Pentose fermentation by a recombinant microorganism

Also Published As

Publication number Publication date
MX350005B (es) 2017-08-22
NO2812430T3 (ru) 2018-02-03
WO2013117631A1 (en) 2013-08-15
HUE035703T2 (en) 2018-05-28
AU2013218001A1 (en) 2014-08-28
BR112014019578B1 (pt) 2022-10-11
DK2812430T3 (en) 2017-10-23
CA2863734A1 (en) 2013-08-15
RS56569B1 (sr) 2018-02-28
EP2626423A1 (en) 2013-08-14
BR112014019578A8 (pt) 2017-07-11
ES2643046T3 (es) 2017-11-21
MY171359A (en) 2019-10-10
US20160312177A1 (en) 2016-10-27
CN104204202A (zh) 2014-12-10
PL2812430T3 (pl) 2018-02-28
CA2863734C (en) 2020-03-24
EP2812430A1 (en) 2014-12-17
ZA201405761B (en) 2015-11-25
MX2014009496A (es) 2014-09-11
US20140377813A1 (en) 2014-12-25
EA035000B1 (ru) 2020-04-16
SI2812430T1 (en) 2018-03-30
EP2812430B1 (en) 2017-09-06
AU2013218001B2 (en) 2016-02-18
BR112014019578A2 (en) 2019-10-15
HRP20171583T1 (hr) 2017-12-01
CN104204202B (zh) 2017-08-01

Similar Documents

Publication Publication Date Title
EA202090067A2 (ru) Способ получения рекомбинантного adamts13 в культуре клеток
EA201291024A1 (ru) Композиции эндорибонуклеаз и способы их использования
EA202090805A1 (ru) Клетки для получения рекомбинантной идуронат-2-сульфатазы
ES2570382T3 (es) Composiciones y métodos para la producción de azúcares fermentables
WO2011159684A3 (en) Generation of induced pluripotent stem cells from small volumes of peripheral blood
EA201300054A1 (ru) Полипептид, обладающий активностью бета-глюкозидазы, и его применение
EA201270187A1 (ru) Ферментативное продуцирование этанола, не содержащего глицерин
NZ592726A (en) Amnion derived adherent cells
MX2013005654A (es) Composiciones y metodos para la produccion de acido 3-hidroxipropionico.
EA201001435A1 (ru) Клетка, ферментирующая сахар-пентозу
EA201171018A1 (ru) Биореактор для культивирования клеток млекопитающих
AR087980A1 (es) Actividad de dnasa endogena para reducir el contenido de adn
EA201201126A1 (ru) Полипептид, обладающий активностью целлобиогидролазы, и его применение
EA201100225A1 (ru) Новые регуляторные элементы
RU2017113926A (ru) Композиция для получения тагатозы и способ получения тагатозы из фруктозы
WO2013012824A3 (en) Trichoplusia ni piggybac transposases with reduced integration activity
AR083354A1 (es) Polipeptidos variantes cbh i (celobiohidrolasas i) con reducida inhibicion de producto
WO2013016724A3 (en) Decarboxylase proteins with high keto-isovalerate decarboxylase activity
EA201400877A1 (ru) Микроорганизмы, ферментирующие пентозы
EA201590794A1 (ru) Рекомбинантные нейротоксины clostridium botulinum
MX373941B (es) Microorganismo capaz de producir l-aminoacido, y un metodo para producir l-aminoacido usando el mismo.
EA201300057A1 (ru) Полипептид, обладающий углеводдеградирующей активностью, и его применения
IN2015DN01042A (ru)
EA201300059A1 (ru) Полипептид, обладающий активностью сволленина, и его применение
IN2015DN01041A (ru)