DK2855674T3 - Chymosinvarianter med forbedrede mælkekoagulerende egenskaber - Google Patents

Chymosinvarianter med forbedrede mælkekoagulerende egenskaber Download PDF

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Publication number
DK2855674T3
DK2855674T3 DK13727539.2T DK13727539T DK2855674T3 DK 2855674 T3 DK2855674 T3 DK 2855674T3 DK 13727539 T DK13727539 T DK 13727539T DK 2855674 T3 DK2855674 T3 DK 2855674T3
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DK
Denmark
Prior art keywords
improved milk
chymosin variants
coagulant properties
milk coagulant
properties
Prior art date
Application number
DK13727539.2T
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English (en)
Other versions
DK2855674T4 (da
Inventor
Martin Lund
Jonas Jacobsen
Sari Charlotte Hansen
Iben Jeppesen
Den Brink Johannes Maarten Van
Original Assignee
Chr Hansen As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=48577692&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK2855674(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Chr Hansen As filed Critical Chr Hansen As
Publication of DK2855674T3 publication Critical patent/DK2855674T3/da
Application granted granted Critical
Publication of DK2855674T4 publication Critical patent/DK2855674T4/da

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Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/1203Addition of, or treatment with, enzymes or microorganisms other than lactobacteriaceae
    • A23C9/1209Proteolytic or milk coagulating enzymes, e.g. trypsine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478Aspartic endopeptidases (3.4.23)
    • C12N9/6483Chymosin (3.4.23.4), i.e. rennin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C11/00Milk substitutes, e.g. coffee whitener compositions
    • A23C11/02Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins
    • A23C11/10Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins
    • A23C11/103Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins containing only proteins from pulses, oilseeds or nuts, e.g. nut milk
    • A23C11/106Addition of, or treatment with, microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C19/00Cheese; Cheese preparations; Making thereof
    • A23C19/02Making cheese curd
    • A23C19/032Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A23C19/0326Rennet produced by fermentation, e.g. microbial rennet; Rennet produced by genetic engineering
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C20/00Cheese substitutes
    • A23C20/02Cheese substitutes containing neither milk components, nor caseinate, nor lactose, as sources of fats, proteins or carbohydrates
    • A23C20/025Cheese substitutes containing neither milk components, nor caseinate, nor lactose, as sources of fats, proteins or carbohydrates mainly containing proteins from pulses or oilseeds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/189Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L11/00Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/40Pulse curds
    • A23L11/45Soy bean curds, e.g. tofu
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L11/00Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/60Drinks from legumes, e.g. lupine drinks
    • A23L11/65Soy drinks
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23Aspartic endopeptidases (3.4.23)
    • C12Y304/23004Chymosin (3.4.23.4), i.e. rennin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00Production
    • C12N2330/50Biochemical production, i.e. in a transformed host cell

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Polymers & Plastics (AREA)
  • Organic Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Nutrition Science (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Animal Husbandry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Peptides Or Proteins (AREA)
  • Dairy Products (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
DK13727539.2T 2012-05-25 2013-05-22 Chymosinvarianter med forbedrede mælkekoagulerende egenskaber DK2855674T4 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP12169503 2012-05-25
PCT/EP2013/060460 WO2013174840A1 (en) 2012-05-25 2013-05-22 Variants of chymosin with improved milk-clotting properties

Publications (2)

Publication Number Publication Date
DK2855674T3 true DK2855674T3 (da) 2019-06-24
DK2855674T4 DK2855674T4 (da) 2022-11-21

Family

ID=48577692

Family Applications (2)

Application Number Title Priority Date Filing Date
DK19174281.6T DK3604509T3 (da) 2012-05-25 2013-05-22 Chymosinvarianter med forbedrede mælkekoagulationsegenskaber
DK13727539.2T DK2855674T4 (da) 2012-05-25 2013-05-22 Chymosinvarianter med forbedrede mælkekoagulerende egenskaber

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK19174281.6T DK3604509T3 (da) 2012-05-25 2013-05-22 Chymosinvarianter med forbedrede mælkekoagulationsegenskaber

Country Status (19)

Country Link
US (2) US9930899B2 (da)
EP (3) EP2855674B2 (da)
JP (3) JP2015518718A (da)
KR (2) KR102245756B1 (da)
CN (2) CN110714001A (da)
AU (3) AU2013265343B2 (da)
BR (2) BR112014029012B1 (da)
CA (3) CA3100606C (da)
DK (2) DK3604509T3 (da)
ES (1) ES2741723T5 (da)
FI (1) FI2855674T4 (da)
HK (1) HK1202894A1 (da)
MX (3) MX377155B (da)
NZ (4) NZ702030A (da)
PL (2) PL2855674T5 (da)
RU (2) RU2683385C2 (da)
TR (1) TR201910420T4 (da)
UA (2) UA126330C2 (da)
WO (1) WO2013174840A1 (da)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ702030A (en) 2012-05-25 2016-11-25 Chr Hansen As Variants of chymosin with improved milk-clotting properties
RU2717770C2 (ru) 2014-02-26 2020-03-25 Кхр. Хансен А/С Варианты химозина с улучшенными молокосвертывающими свойствами
US20180110234A1 (en) 2015-02-10 2018-04-26 Chr. Hansen A/S Blends of chymosins with improved milk-clotting properties
RU2740317C2 (ru) * 2015-06-22 2021-01-13 Кхр. Хансен А/С Варианты химозина с улучшенными свойствами
EP3344049A1 (en) * 2015-08-31 2018-07-11 Chr. Hansen A/S Variants of chymosin with improved properties
EP3457859A1 (en) * 2016-05-19 2019-03-27 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
US10961524B2 (en) 2016-05-19 2021-03-30 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
CN106754840B (zh) * 2016-12-22 2019-06-21 江南大学 一种酶活及热稳定性改善的凝乳酶突变体
US20200345022A1 (en) 2018-01-15 2020-11-05 Chr. Hansen A/S Fermented milk product and preparation thereof using phospholipase
CN111172139B (zh) * 2020-02-27 2021-11-30 武汉轻工大学 一种小牛皱胃凝乳酶及基因与菌株和应用
CN113801867B (zh) * 2021-11-18 2022-03-18 中国农业科学院生物技术研究所 骆驼凝乳酶原突变蛋白及其编码基因和应用
US20260000087A1 (en) 2022-04-06 2026-01-01 Chr. Hansen A/S Aspartic Protease, Methods and Uses Thereof

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0123928A3 (en) 1983-03-31 1986-02-05 Codon Genetic Engineering Laboratories Recombinant dna coding for a polypeptide displaying milk clotting activity
US7390936B1 (en) 1999-08-23 2008-06-24 Sembiosys Genetics Inc. Commercial production of chymosin in plants
RU2192137C2 (ru) * 2000-08-07 2002-11-10 Открытое акционерное общество "Уфамолагропром" Способ производства сыра
DK1334182T4 (da) 2000-11-06 2021-04-26 Eidgenoessische Technische Hochschule Eth Fremgangsmåde til fremstilling af ikke-bovint chymosin og anvendelse heraf
AU2003282724B2 (en) 2002-10-02 2010-03-04 Catalyst Biosciences, Inc. Methods of generating and screening for proteases with altered specificity
BRPI0412007A (pt) 2003-06-27 2006-08-15 Bioren Inc mutagênese look-through
EP1745068A4 (en) 2004-03-30 2009-11-04 Sudershan Biotech Ltd RECOMBINANT CALM CHYMOSIN AND PROCESS FOR PRODUCING THE SAME
EP3763220A1 (en) 2007-02-13 2021-01-13 Chr. Hansen A/S Coagulation of milk
JP2010046034A (ja) 2008-08-22 2010-03-04 Toyota Central R&D Labs Inc 変異体のスクリーニング方法
EP2411512B1 (en) 2009-03-24 2013-11-20 Meito Sangyo Co., Ltd. Improved type milk-clotting protease derived from a microorganism
EA035275B1 (ru) 2012-05-03 2020-05-22 ДСМ АйПи АССЕТС Б.В. Варианты химозина с повышенной специфичностью и их применение в получении сыра
NZ702030A (en) 2012-05-25 2016-11-25 Chr Hansen As Variants of chymosin with improved milk-clotting properties
RU2717770C2 (ru) 2014-02-26 2020-03-25 Кхр. Хансен А/С Варианты химозина с улучшенными молокосвертывающими свойствами
US20180110234A1 (en) 2015-02-10 2018-04-26 Chr. Hansen A/S Blends of chymosins with improved milk-clotting properties
RU2740317C2 (ru) 2015-06-22 2021-01-13 Кхр. Хансен А/С Варианты химозина с улучшенными свойствами
EP3344049A1 (en) 2015-08-31 2018-07-11 Chr. Hansen A/S Variants of chymosin with improved properties
EP3457859A1 (en) 2016-05-19 2019-03-27 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
US10961524B2 (en) 2016-05-19 2021-03-30 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties

Also Published As

Publication number Publication date
NZ725398A (en) 2018-05-25
NZ742660A (en) 2019-12-20
PL2855674T3 (pl) 2019-11-29
RU2019108268A3 (da) 2021-11-29
WO2013174840A1 (en) 2013-11-28
EP3929288A1 (en) 2021-12-29
AU2013265343B2 (en) 2019-02-21
MX2014014300A (es) 2015-02-12
JP2020058367A (ja) 2020-04-16
EP2855674B1 (en) 2019-05-15
PL2855674T5 (pl) 2023-03-27
EP3604509B1 (en) 2021-03-31
KR102245756B1 (ko) 2021-04-29
CA3100606C (en) 2023-08-01
CA2874214A1 (en) 2013-11-28
MX366610B (es) 2019-07-16
BR112014029012A2 (pt) 2017-06-27
NZ742745A (en) 2019-12-20
US20150173383A1 (en) 2015-06-25
CA2874214C (en) 2021-02-16
DK3604509T3 (da) 2021-05-25
US20180317510A1 (en) 2018-11-08
MX377155B (es) 2025-03-07
RU2019108268A (ru) 2019-04-23
KR20150015014A (ko) 2015-02-09
AU2019203589B2 (en) 2021-11-04
ES2741723T3 (es) 2020-02-12
UA118956C2 (uk) 2019-04-10
US9930899B2 (en) 2018-04-03
EP2855674A1 (en) 2015-04-08
HK1202894A1 (en) 2015-10-09
CA3201812A1 (en) 2013-11-28
CN110714001A (zh) 2020-01-21
ES2741723T5 (es) 2023-01-23
MX2019008255A (es) 2020-01-15
AU2013265343A1 (en) 2014-12-04
KR102419515B1 (ko) 2022-07-11
BR112014029012B1 (pt) 2022-05-03
CN104487572B (zh) 2019-12-13
JP2018148926A (ja) 2018-09-27
EP3604509A1 (en) 2020-02-05
MX2020011941A (es) 2021-01-15
RU2014152723A (ru) 2016-07-20
KR20210048580A (ko) 2021-05-03
JP2015518718A (ja) 2015-07-06
PL3604509T3 (pl) 2021-09-13
BR122021019895B1 (pt) 2022-10-25
AU2022200663B2 (en) 2024-10-31
CA3100606A1 (en) 2013-11-28
TR201910420T4 (tr) 2019-08-21
DK2855674T4 (da) 2022-11-21
EP2855674B2 (en) 2022-11-02
AU2019203589A1 (en) 2019-06-13
US10806157B2 (en) 2020-10-20
NZ702030A (en) 2016-11-25
CN104487572A (zh) 2015-04-01
AU2022200663A1 (en) 2022-02-24
UA126330C2 (uk) 2022-09-21
RU2683385C2 (ru) 2019-03-28
FI2855674T4 (fi) 2023-01-13

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