DK2872629T3 - Tm-forstærkede blokeringsoligonukleotider og lokkemidler til forbedret target-berigesle og reduceret off-target-udvælgelse - Google Patents

Tm-forstærkede blokeringsoligonukleotider og lokkemidler til forbedret target-berigesle og reduceret off-target-udvælgelse Download PDF

Info

Publication number
DK2872629T3
DK2872629T3 DK13737778T DK13737778T DK2872629T3 DK 2872629 T3 DK2872629 T3 DK 2872629T3 DK 13737778 T DK13737778 T DK 13737778T DK 13737778 T DK13737778 T DK 13737778T DK 2872629 T3 DK2872629 T3 DK 2872629T3
Authority
DK
Denmark
Prior art keywords
target
lurgets
oigonucleotides
enhanced
reduced
Prior art date
Application number
DK13737778T
Other languages
English (en)
Inventor
Mark Aaron Behlke
John Robert Havens
Scott Daniel Rose
Zachary Zwirko
Doron Lipson
Frank Soo Juhn
Mirna Jarosz
Original Assignee
Integrated Dna Tech Inc
Found Medicine Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Integrated Dna Tech Inc, Found Medicine Inc filed Critical Integrated Dna Tech Inc
Application granted granted Critical
Publication of DK2872629T3 publication Critical patent/DK2872629T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
DK13737778T 2012-07-03 2013-07-03 Tm-forstærkede blokeringsoligonukleotider og lokkemidler til forbedret target-berigesle og reduceret off-target-udvælgelse DK2872629T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261667919P 2012-07-03 2012-07-03
US201261745435P 2012-12-21 2012-12-21
PCT/US2013/049402 WO2014008447A1 (en) 2012-07-03 2013-07-03 Tm-enhanced blocking oligonucleotides and baits for improved target enrichment and reduced off-target selection

Publications (1)

Publication Number Publication Date
DK2872629T3 true DK2872629T3 (da) 2019-12-09

Family

ID=48794242

Family Applications (1)

Application Number Title Priority Date Filing Date
DK13737778T DK2872629T3 (da) 2012-07-03 2013-07-03 Tm-forstærkede blokeringsoligonukleotider og lokkemidler til forbedret target-berigesle og reduceret off-target-udvælgelse

Country Status (9)

Country Link
US (4) US10266889B2 (da)
EP (1) EP2872629B1 (da)
JP (4) JP6433893B2 (da)
AU (1) AU2013286635B2 (da)
CA (1) CA2877740A1 (da)
DK (1) DK2872629T3 (da)
ES (1) ES2769796T3 (da)
SG (2) SG10201610861XA (da)
WO (1) WO2014008447A1 (da)

Families Citing this family (86)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20140024270A (ko) 2010-12-30 2014-02-28 파운데이션 메디신 인코포레이티드 종양 샘플의 다유전자 분석의 최적화
US9074204B2 (en) * 2011-05-20 2015-07-07 Fluidigm Corporation Nucleic acid encoding reactions
EP2825675B1 (en) * 2012-03-13 2017-12-27 Patel, Abhijit Ajit Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
SG10201610861XA (en) 2012-07-03 2017-02-27 Integrated Dna Tech Inc Tm-enhanced blocking oligonucleotides and baits for improved target enrichment and reduced off-target selection
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
EP3561072A1 (en) 2012-12-10 2019-10-30 Resolution Bioscience, Inc. Methods for targeted genomic analysis
TWI707038B (zh) 2013-08-05 2020-10-11 美商扭轉生物科技有限公司 重新合成之基因庫
WO2015105993A1 (en) * 2014-01-09 2015-07-16 AgBiome, Inc. High throughput discovery of new genes from complex mixtures of environmental microbes
US9587268B2 (en) * 2014-01-29 2017-03-07 Agilent Technologies Inc. Fast hybridization for next generation sequencing target enrichment
US9670485B2 (en) * 2014-02-15 2017-06-06 The Board Of Trustees Of The Leland Stanford Junior University Partitioning of DNA sequencing libraries into host and microbial components
EP3919621A1 (en) 2014-06-23 2021-12-08 The General Hospital Corporation Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq)
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
US10648103B2 (en) 2014-10-10 2020-05-12 Invitae Corporation Universal blocking oligo system and improved hybridization capture methods for multiplexed capture reactions
CA2967447C (en) 2014-12-05 2025-07-08 Foundation Medicine, Inc. MULTIGEN ANALYSIS OF TUMOR SAMPLES
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US10429342B2 (en) 2014-12-18 2019-10-01 Edico Genome Corporation Chemically-sensitive field effect transistor
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
AU2016250529A1 (en) 2015-04-20 2017-11-30 Neogenomics Laboratories, Inc. Method to increase sensitivity of next generation sequencing
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
CA2986685C (en) 2015-05-27 2024-05-28 Quest Diagnostics Investments Incorporated Compositions and methods for screening solid tumors
KR101858344B1 (ko) * 2015-06-01 2018-05-16 연세대학교 산학협력단 바코드 서열을 포함하는 어댑터를 이용한 차세대 염기서열 분석 방법
EP3347466B9 (en) 2015-09-08 2024-06-26 Cold Spring Harbor Laboratory Genetic copy number determination using high throughput multiplex sequencing of smashed nucleotides
KR20180050411A (ko) 2015-09-18 2018-05-14 트위스트 바이오사이언스 코포레이션 올리고핵산 변이체 라이브러리 및 그의 합성
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
US10577643B2 (en) * 2015-10-07 2020-03-03 Illumina, Inc. Off-target capture reduction in sequencing techniques
CA3004435A1 (en) 2015-11-11 2017-05-18 Resolution Bioscience, Inc. High efficiency construction of dna libraries
WO2017095958A1 (en) 2015-12-01 2017-06-08 Twist Bioscience Corporation Functionalized surfaces and preparation thereof
CN108603224B (zh) 2015-12-16 2022-06-28 富鲁达公司 高水平多路复用扩增
WO2017168329A1 (en) * 2016-03-28 2017-10-05 Boreal Genomics, Inc. Droplet-based linked-fragment sequencing
WO2017180592A1 (en) * 2016-04-11 2017-10-19 President And Fellows Of Harvard College Sequence design for efficient assembly of nucleic acid structures
US11149312B2 (en) * 2016-04-15 2021-10-19 University Health Network Hybrid-capture sequencing for determining immune cell clonality
EP3459115A4 (en) 2016-05-16 2020-04-08 Agilome, Inc. GRAPHENE FETING DEVICES, SYSTEMS, AND METHODS OF USE THEREOF FOR NUCLEIC ACID SEQUENCING
KR102212257B1 (ko) 2016-08-22 2021-02-04 트위스트 바이오사이언스 코포레이션 드 노보 합성된 핵산 라이브러리
JP7217224B2 (ja) 2016-08-25 2023-02-02 レゾリューション バイオサイエンス, インコーポレイテッド Dna試料中のゲノムコピー変化の検出方法
JP6997773B2 (ja) * 2016-09-15 2022-01-18 アーチャーディーエックス, エルエルシー 無細胞dnaの分析用の核酸サンプル調製の方法
CA3037185A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation
WO2018057526A2 (en) 2016-09-21 2018-03-29 Twist Bioscience Corporation Nucleic acid based data storage
CN110191959B (zh) 2016-11-02 2024-04-09 阿谢尔德克斯有限责任公司 用于免疫组库测序的核酸样品制备方法
CA3048420C (en) 2016-12-09 2025-05-20 Ncan Genomics, Inc. Bound ligature
EP3554514A4 (en) 2016-12-16 2020-08-05 Twist Bioscience Corporation BANKS OF VARIANTS OF IMMUNOLOGICAL SYNAPSE AND THEIR SYNTHESIS
EP3559255A1 (en) 2016-12-23 2019-10-30 Grail, Inc. Methods for high efficiency library preparation using double-stranded adapters
CN110892485B (zh) 2017-02-22 2024-03-22 特韦斯特生物科学公司 基于核酸的数据存储
AU2018234629A1 (en) 2017-03-15 2019-10-17 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018183918A1 (en) * 2017-03-30 2018-10-04 Grail, Inc. Enhanced ligation in sequencing library preparation
US11118222B2 (en) 2017-03-31 2021-09-14 Grail, Inc. Higher target capture efficiency using probe extension
US12473587B2 (en) * 2017-04-06 2025-11-18 Complete Omics Inc. Nucleic acid capture method
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US11053537B2 (en) * 2017-05-25 2021-07-06 Integrated Dna Technologies, Inc. Reverse complement adapters for the mitigation of UMI hopping
JP7169999B2 (ja) 2017-06-12 2022-11-11 ツイスト バイオサイエンス コーポレーション シームレス核酸アセンブリのための方法
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
AU2018328847A1 (en) 2017-09-11 2020-04-23 Twist Bioscience Corporation GPCR binding proteins and synthesis thereof
EP4269583B1 (en) * 2017-09-28 2025-12-31 Grail, Inc. ENRICHEMENT OF SHORT NUCLEIC ACID FRAGMENTS IN SEQUENCING BANK PREPARATION
WO2019075197A1 (en) 2017-10-11 2019-04-18 The General Hospital Corporation METHODS OF DETECTION OF INDIVIDUAL SITE-SPECIFIC PARASITE GENOMIC DEAMINATION BY BASE EDITING TECHNOLOGIES
EP3697798A4 (en) * 2017-10-18 2021-08-11 Day Zero Diagnostics, Inc. SELECTIVE ENRICHMENT OF A POPULATION OF DNA IN A MIXED DNA SAMPLE BY TARGETED DELETION OF DNA AMPLIFICATION
WO2019079769A1 (en) 2017-10-20 2019-04-25 Twist Bioscience Corporation HEATED NANOWELLS FOR THE SYNTHESIS OF POLYNUCLEOTIDES
EP4715681A2 (en) 2018-01-04 2026-03-25 Atlas Data Storage, Inc. Dna-based digital information storage
EP3781585A4 (en) 2018-04-17 2022-01-26 The General Hospital Corporation SENSITIVE IN VITRO TESTS FOR SUBSTRATE PREFERENCES AND SITE FOR NUCLEIC ACID BINDERS, MODIFIERS AND CLEAVAGE AGENTS
IL278771B2 (en) 2018-05-18 2025-09-01 Twist Bioscience Corp Polynucleotides, reagents and methods for nucleic acid hybridization
US12584163B2 (en) 2018-06-11 2026-03-24 Foundation Medicine, Inc. Compositions and methods for evaluating genomic alterations
CN108949941A (zh) * 2018-06-25 2018-12-07 北京莲和医学检验所有限公司 低频突变检测方法、试剂盒和装置
WO2020033394A1 (en) * 2018-08-06 2020-02-13 Northwestern University Enhanced capture of target nucleic acids
CA3109238A1 (en) 2018-08-15 2020-02-20 Illumina, Inc. Compositions and methods for improving library enrichment
WO2020139871A1 (en) 2018-12-26 2020-07-02 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
CN110699426B (zh) * 2019-01-02 2022-01-28 上海臻迪基因科技有限公司 基因目标区域富集方法及试剂盒
WO2020176362A1 (en) * 2019-02-25 2020-09-03 Twist Bioscience Corporation Compositions and methods for next generation sequencing
WO2020176678A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for glp1 receptor
AU2020227802A1 (en) 2019-02-26 2021-10-14 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
CA3144644A1 (en) 2019-06-21 2020-12-24 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
CN110409001B (zh) * 2019-07-25 2022-11-15 北京贝瑞和康生物技术有限公司 一种构建捕获文库的方法和试剂盒
CN110527715A (zh) * 2019-09-16 2019-12-03 中国科学院遗传与发育生物学研究所农业资源研究中心 一种功能基因组克隆子库的测序方法
US12091777B2 (en) 2019-09-23 2024-09-17 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2
EP4034564A4 (en) 2019-09-23 2023-12-13 Twist Bioscience Corporation VARIANT NUCLEIC ACID LIBRARIES FOR SINGLE-DOMAIN ANTIBODIES
BR112022011235A2 (pt) 2019-12-09 2022-12-13 Twist Bioscience Corp Bibliotecas de variantes de ácido nucleico para receptores de adenosina
EP3851542A1 (en) 2020-01-20 2021-07-21 Tecan Genomics, Inc. Depletion of abundant uninformative sequences
CN113275053A (zh) 2020-02-03 2021-08-20 帝肯基因组学公司 试剂存储系统
WO2021195324A1 (en) * 2020-03-26 2021-09-30 Integrated Dna Technologies, Inc. Hybridization capture methods and compositions
US20220154256A1 (en) * 2020-11-19 2022-05-19 Regeneron Pharmaceuticals, Inc. Genotyping By Sequencing
US12378596B2 (en) * 2020-12-03 2025-08-05 Roche Sequencing Solutions, Inc. Whole transcriptome analysis in single cells
KR20230123872A (ko) * 2020-12-21 2023-08-24 일루미나, 인코포레이티드 변형된 포획 프라이머를 사용하여 표적 폴리뉴클레오티드를 포획 및 증폭시키기 위한 조성물 및 방법
CN117730155A (zh) * 2021-05-24 2024-03-19 豪夫迈·罗氏有限公司 用于核酸杂交的增强子寡核苷酸
US20240296903A1 (en) * 2021-07-06 2024-09-05 Switch Therapeutics Inc. Methods of placing locked nucleic acids in small interfering rna strands
CN117940578A (zh) 2021-09-03 2024-04-26 因美纳有限公司 含有氟化碳链的杂交探针和相关方法

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6110676A (en) * 1996-12-04 2000-08-29 Boston Probes, Inc. Methods for suppressing the binding of detectable probes to non-target sequences in hybridization assays
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
US6936443B2 (en) * 2000-04-03 2005-08-30 Cytyc Corporation Detection and typing of human papillomavirus using PNA probes
WO2003014734A1 (fr) * 2001-08-07 2003-02-20 Keio University Procede de detection d'une interaction entre une substance et une proteine, procede d'analyse de la proteine interagissant avec la substance et procede de formation d'un complexe contenant la substance et la proteine interagissant avec cette substance
FR2842534B1 (fr) 2002-07-19 2006-01-20 Inst Nat Sante Rech Med Amplification multiplex quantitative a l'echelle d'un genome, et applications a la detection de remaniements genomiques
US20040022764A1 (en) * 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
US20050181394A1 (en) * 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
ATE461292T1 (de) * 2003-09-10 2010-04-15 Althea Technologies Inc Erstellung von expressionsprofilen unter verwendung von mikroarrays
US7407753B2 (en) * 2004-05-24 2008-08-05 Yissum Research Development Company Of The Hebrewuniversityofjerusalem Methods, kits and pharmaceutical compositions for diagnosing, delaying onset of, preventing and/or treating osteoporosis
US7867703B2 (en) 2004-08-26 2011-01-11 Agilent Technologies, Inc. Element defined sequence complexity reduction
US20070111960A1 (en) * 2005-03-04 2007-05-17 Advandx, Inc. High affinity probes for analysis of human papillomavirus expression
WO2007117256A1 (en) 2005-05-31 2007-10-18 Applera Corporation Multiplexed amplification of short nucleic acids
US20090068643A1 (en) * 2005-11-23 2009-03-12 Integrated Dna Technologies, Inc. Dual Function Primers for Amplifying DNA and Methods of Use
US20100009355A1 (en) * 2006-03-14 2010-01-14 Kolodney Michael S Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides
WO2008143972A2 (en) * 2007-05-18 2008-11-27 Advandx, Inc. Detection of methicillin-resistant staphylococcus aureus
EP2245198A1 (en) 2008-02-04 2010-11-03 Massachusetts Institute of Technology Selection of nucleic acids by solution hybridization to oligonucleotide baits
US20110287424A1 (en) * 2009-03-27 2011-11-24 Life Technologies Corporation Methylation-specific competitive allele-specific taqman polymerase chain reaction (cast-pcr)
EP3002337B1 (en) * 2009-03-30 2018-10-24 Illumina, Inc. Gene expression analysis in single cells
EP2270203A1 (en) 2009-06-29 2011-01-05 AIT Austrian Institute of Technology GmbH Oligonucleotide hybridization method
DK2459743T3 (da) 2009-07-30 2017-11-06 Hoffmann La Roche Sæt af oligonukleotidprober såvel som fremgangsmåder og anvendelser relateret dertil
US20110091939A1 (en) * 2009-10-20 2011-04-21 Longze Cui Methods and Compositions for Removing Specific Target Nucleic Acids
EP2494066B1 (en) * 2009-10-27 2017-04-05 Swift Biosciences, Inc. Bimolecular primers
US20110111462A1 (en) * 2009-11-06 2011-05-12 Stephen Picone Composition and Method for Synthesizing a Deoxyribonucleotide Chain Using a Double Stranded Nucleic Acid Complex with a Thermostable Polymerase
US20110117559A1 (en) * 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
US8278049B2 (en) * 2010-04-26 2012-10-02 Ann & Robert H. Lurie Children's Hospital of Chicago Selective enrichment of CpG islands
CN101921874B (zh) * 2010-06-30 2013-09-11 深圳华大基因科技有限公司 基于Solexa测序法的检测人类乳头瘤病毒的方法
US9081737B2 (en) 2010-08-02 2015-07-14 Integrated Dna Technologies, Inc. Methods for predicting stability and melting temperatures of nucleic acid duplexes
CN101967476B (zh) * 2010-09-21 2012-11-14 深圳华大基因科技有限公司 一种基于接头连接的DNA PCR-Free标签文库构建方法
AU2011319755B2 (en) * 2010-10-27 2017-02-23 President And Fellows Of Harvard College Compositions of toehold primer duplexes and methods of use
US20130230857A1 (en) 2010-11-05 2013-09-05 The Broad Institute, Inc. Hybrid selection using genome-wide baits for selective genome enrichment in mixed samples
KR20140024270A (ko) 2010-12-30 2014-02-28 파운데이션 메디신 인코포레이티드 종양 샘플의 다유전자 분석의 최적화
US10196681B2 (en) * 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
SG10201610861XA (en) * 2012-07-03 2017-02-27 Integrated Dna Tech Inc Tm-enhanced blocking oligonucleotides and baits for improved target enrichment and reduced off-target selection

Also Published As

Publication number Publication date
US20170114404A1 (en) 2017-04-27
US20170096706A1 (en) 2017-04-06
US11566283B2 (en) 2023-01-31
CA2877740A1 (en) 2014-01-09
AU2013286635B2 (en) 2018-11-08
JP6433893B2 (ja) 2018-12-05
JP2021104032A (ja) 2021-07-26
US11566282B2 (en) 2023-01-31
SG10201610861XA (en) 2017-02-27
US20230357841A1 (en) 2023-11-09
EP2872629A1 (en) 2015-05-20
WO2014008447A1 (en) 2014-01-09
JP2015521853A (ja) 2015-08-03
JP2019037248A (ja) 2019-03-14
JP7459010B2 (ja) 2024-04-01
US20140031240A1 (en) 2014-01-30
JP2021104031A (ja) 2021-07-26
SG11201408807YA (en) 2015-01-29
JP7358413B2 (ja) 2023-10-10
EP2872629B1 (en) 2019-09-04
US10266889B2 (en) 2019-04-23
AU2013286635A1 (en) 2015-01-22
ES2769796T3 (es) 2020-06-29

Similar Documents

Publication Publication Date Title
DK2854883T3 (da) Biomatrix-hydrogeler og fremgangsmåder til anvendelse deraf
DK3468197T3 (da) Fremgangsmåde til interforudsigelse og indretning dertil
DK2925782T3 (da) Anti-cd47-antistoffer og fremgangsmåder til anvendelse deraf
DK2852680T3 (da) Fremgangsmåder og processer til ikke-invasiv evaluering af genetiske variationer
DK3196022T3 (da) Trykningssystem og fremgangsmåde
DK3421051T3 (da) Fremgangsmåder til glycokonjugering og sammensætninger
DK3550836T3 (da) Fremgangsmåde og anordning til afkodning
DK2573542T3 (da) Automatiseret farvningssystem og reaktionskammer
DK2855736T3 (da) Elektrolysator og energisystem
DK2906684T3 (da) T-celle-modificerende forbindelser og anvendelser deraf
DK2920167T3 (da) Hydrazinyl-indol-forbindelser og konjugater
DK2850202T3 (da) Fremgangsmåder og grupper
DK3045187T3 (da) Injektor og fremgangsmåde til samling
DK3597644T3 (da) Kationiske aminlipider og anvendelser deraf
DK2857401T5 (da) HIDTIL UKENDT ß-LACTAMASEINHIBITOR OG FREMGANGSMÅDE TIL AT FREMSTILLE SAMME
DK2812443T3 (da) Cd47-antistoffer og fremgangsmåder til anvendelse deraf
DK2692026T3 (da) Forspændingselement til fastholdelse af kontinuitet og tilknyttet fremgangsmåde
DK3308641T3 (da) Immundeficient genmodificeret gnaver og fremgangsmåder til anvendelse deraf
DK2890780T3 (da) Fremgangsmåder og sammensætninger til behandling af en genetisk tilstand
DK2914291T3 (da) Anti-komplement-c1s-antistoffer og anvendelser deraf
DK2838900T3 (da) Forbindelser og fremgangsmåder til antiviral behandling
SG10201802883UA (en) Nucleic acid amplification
DK2758349T3 (da) Vanadiumbaserede frittematerialer og fremgangsmåder til fremstilling heraf
LT2864292T (lt) Neprilizino inhibitoriai
DK3381461T3 (da) Peptider og fremgangsmåder til anvendelse deraf