DK3030661T3 - Øgning af et transgens ekspression i eukaryotiske celler ved reduktion af rna-interferens - Google Patents

Øgning af et transgens ekspression i eukaryotiske celler ved reduktion af rna-interferens Download PDF

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Publication number
DK3030661T3
DK3030661T3 DK14756146.8T DK14756146T DK3030661T3 DK 3030661 T3 DK3030661 T3 DK 3030661T3 DK 14756146 T DK14756146 T DK 14756146T DK 3030661 T3 DK3030661 T3 DK 3030661T3
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Denmark
Prior art keywords
transgens
expression
reduction
increase
rna interference
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DK14756146.8T
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English (en)
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Andrei Miasnikov
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Danisco Us Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/127—RNA-directed RNA polymerase (2.7.7.48), i.e. RNA replicase
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/485—Exopeptidases (3.4.11-3.4.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07048—RNA-directed RNA polymerase (2.7.7.48), i.e. RNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/26—Endoribonucleases producing 5'-phosphomonoesters (3.1.26)
    • C12Y301/26003—Ribonuclease III (3.1.26.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/14—Dipeptidyl-peptidases and tripeptidyl-peptidases (3.4.14)

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  • Health & Medical Sciences (AREA)
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  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK14756146.8T 2013-08-08 2014-07-31 Øgning af et transgens ekspression i eukaryotiske celler ved reduktion af rna-interferens DK3030661T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361863829P 2013-08-08 2013-08-08
PCT/US2014/049159 WO2015020876A1 (en) 2013-08-08 2014-07-31 Increasing the expression of a transgene in eukaryotic cells by reducing rna interference

Publications (1)

Publication Number Publication Date
DK3030661T3 true DK3030661T3 (da) 2019-06-11

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DK14756146.8T DK3030661T3 (da) 2013-08-08 2014-07-31 Øgning af et transgens ekspression i eukaryotiske celler ved reduktion af rna-interferens

Country Status (12)

Country Link
US (1) US10450575B2 (da)
EP (1) EP3030661B1 (da)
CN (1) CN105683380B (da)
CA (1) CA2920564C (da)
DK (1) DK3030661T3 (da)
ES (1) ES2727530T3 (da)
LT (1) LT3030661T (da)
PL (1) PL3030661T3 (da)
PT (1) PT3030661T (da)
SI (1) SI3030661T1 (da)
TR (1) TR201907493T4 (da)
WO (1) WO2015020876A1 (da)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015168733A1 (en) * 2014-05-05 2015-11-12 Queensland University Of Technology Decoy molecules
EP4012018A1 (en) * 2020-11-12 2022-06-15 Clariant Produkte (Deutschland) GmbH Process for the production of a technical enzyme composition with low viscosity produced by a filamentous fungus
WO2023039358A1 (en) 2021-09-09 2023-03-16 Dupont Nutrition Biosciences Aps Over expression of foldases and chaperones improves protein production

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1302885B1 (it) 1998-11-30 2000-10-10 Univ Roma Isolamento e caratterizzazione di un gene per il silenziamento genicoda n. crassa e suoi usi.
IT1306014B1 (it) 1999-02-22 2001-05-23 Univ Roma Isolamento e caratterizzazione di un gene per il silenziamento genicoda n. crassa e suoi usi.
EP1235842A4 (en) * 1999-10-15 2003-04-23 Univ Massachusetts Rna interference pathway genes as tools for targeted genetic interference
GB201012845D0 (en) * 2010-07-30 2010-09-15 Vib Vzw Inhibition of dicer function for treatment of cancer
AU2011302536A1 (en) * 2010-09-15 2013-03-14 The Regents Of The University Of California Thermophilic mutants of Trichoderma reesei endoglucanase I
DK2630249T3 (da) 2010-10-20 2018-12-17 Danisco Us Inc Termostabil trichoderma-cellulase

Also Published As

Publication number Publication date
US10450575B2 (en) 2019-10-22
EP3030661A1 (en) 2016-06-15
PT3030661T (pt) 2019-06-06
PL3030661T3 (pl) 2019-06-28
CN105683380A (zh) 2016-06-15
CA2920564C (en) 2021-11-09
CA2920564A1 (en) 2015-02-12
EP3030661B1 (en) 2019-02-27
LT3030661T (lt) 2019-04-25
WO2015020876A1 (en) 2015-02-12
CN105683380B (zh) 2020-07-03
ES2727530T3 (es) 2019-10-16
TR201907493T4 (tr) 2019-06-21
SI3030661T1 (sl) 2019-06-28
US20160177319A1 (en) 2016-06-23

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