DK320387D0 - Fremgangsmaade til aktivering af genteknologisk fremstillede, heterologe, disulfidbroer indeholdende eukaryotiske proteiner efter ekspression i prokaryotiske celler - Google Patents

Fremgangsmaade til aktivering af genteknologisk fremstillede, heterologe, disulfidbroer indeholdende eukaryotiske proteiner efter ekspression i prokaryotiske celler

Info

Publication number
DK320387D0
DK320387D0 DK320387A DK320387A DK320387D0 DK 320387 D0 DK320387 D0 DK 320387D0 DK 320387 A DK320387 A DK 320387A DK 320387 A DK320387 A DK 320387A DK 320387 D0 DK320387 D0 DK 320387D0
Authority
DK
Denmark
Prior art keywords
expression
pref
activating
gentechnologically
heterologist
Prior art date
Application number
DK320387A
Other languages
English (en)
Other versions
DK175091B1 (da
DK320387A (da
Inventor
Stephan Fischer
Ralf Mattes
Rainer Rudolph
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of DK320387D0 publication Critical patent/DK320387D0/da
Publication of DK320387A publication Critical patent/DK320387A/da
Priority to DK200001897A priority Critical patent/DK175109B1/da
Application granted granted Critical
Publication of DK175091B1 publication Critical patent/DK175091B1/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Lubricants (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
DK198703203A 1985-10-23 1987-06-23 Fremgangsmåde til aktivering af genteknologisk fremstillede, heterologe, disulfidbro-holdige eukaryotiske proteiner efter ekspression i prokaryotiske celler DK175091B1 (da)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DK200001897A DK175109B1 (da) 1985-10-23 2000-12-18 Fremgangsmåde til aktivering af genteknologisk fremstillede, heterologe, disulfidbroer indeholdende eukaryotiske proteiner efter ekspression i prokaryotiske celler

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE3537708 1985-10-23
DE19853537708 DE3537708A1 (de) 1985-10-23 1985-10-23 Verfahren zur aktivierung von t-pa nach expression in prokaryonten
PCT/EP1986/000610 WO1987002673A2 (en) 1985-10-23 1986-10-23 Process for activating heterologous, eucaryotic proteins genetically engineered and presenting disulphide bridges after their expression in procaryotic cells
EP8600610 1986-10-23

Publications (3)

Publication Number Publication Date
DK320387D0 true DK320387D0 (da) 1987-06-23
DK320387A DK320387A (da) 1987-06-23
DK175091B1 DK175091B1 (da) 2004-05-24

Family

ID=6284269

Family Applications (2)

Application Number Title Priority Date Filing Date
DK198703203A DK175091B1 (da) 1985-10-23 1987-06-23 Fremgangsmåde til aktivering af genteknologisk fremstillede, heterologe, disulfidbro-holdige eukaryotiske proteiner efter ekspression i prokaryotiske celler
DK200001897A DK175109B1 (da) 1985-10-23 2000-12-18 Fremgangsmåde til aktivering af genteknologisk fremstillede, heterologe, disulfidbroer indeholdende eukaryotiske proteiner efter ekspression i prokaryotiske celler

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK200001897A DK175109B1 (da) 1985-10-23 2000-12-18 Fremgangsmåde til aktivering af genteknologisk fremstillede, heterologe, disulfidbroer indeholdende eukaryotiske proteiner efter ekspression i prokaryotiske celler

Country Status (26)

Country Link
EP (3) EP0393725B1 (da)
JP (2) JPH0728745B2 (da)
KR (1) KR900009139B1 (da)
AT (2) ATE131489T1 (da)
AU (2) AU590029B2 (da)
CA (1) CA1329157C (da)
CZ (1) CZ280727B6 (da)
DD (1) DD260517A5 (da)
DE (3) DE3537708A1 (da)
DK (2) DK175091B1 (da)
ES (2) ES2061434T3 (da)
FI (2) FI94050C (da)
GR (2) GR920300062T1 (da)
HK (2) HK153596A (da)
HR (1) HRP921075B1 (da)
HU (2) HUT43643A (da)
IE (1) IE62634B1 (da)
IL (1) IL80325A (da)
PT (1) PT83609B (da)
SI (1) SI8611796B (da)
SK (1) SK752686A3 (da)
SU (1) SU1607689A3 (da)
UA (1) UA6023A1 (da)
WO (1) WO1987002673A2 (da)
YU (1) YU47185B (da)
ZA (1) ZA868012B (da)

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JP2581668B2 (ja) * 1985-11-27 1997-02-12 三井東圧化学株式会社 ヒト正常細胞由来のヒト組織プラスミノ−ゲン活性化因子をコ−ドする新しいｄｎａ配列とそれを含むベクタ−及び細胞
US4777043A (en) * 1985-12-17 1988-10-11 Genentech, Inc. Stabilized human tissue plasminogen activator compositions
AU621051B2 (en) * 1987-04-28 1992-03-05 Amgen, Inc. Method for purifying granulocyte-macrophage colony stimulating factor
DE3722082A1 (de) * 1987-07-03 1989-01-12 Behringwerke Ag Verfahren zur bestimmung der aktivitaet von serinproteasen oder serinproteaseinhibitoren
CA1340586C (en) * 1988-09-23 1999-06-08 Cetus Corporation Process for recovering microbially produced interferon-beta
DE3832898A1 (de) * 1988-09-28 1990-04-12 Boehringer Mannheim Gmbh Praeparat von in prokaryonten exprimiertem plasminogenaktivator
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
DE3903581A1 (de) * 1989-02-07 1990-08-16 Boehringer Mannheim Gmbh Gewebs-plasminogenaktivator-derivat
DE3942143A1 (de) * 1989-12-20 1991-06-27 Boehringer Mannheim Gmbh T-pa pro stabilisierung
WO1992003477A1 (en) * 1990-08-20 1992-03-05 Novo Nordisk A/S Biologically active compound, a process for the preparation thereof and use of the same
AU664021B2 (en) * 1990-09-05 1995-11-02 Natinco Nv Solubilization of proteins in active forms
DE4037196A1 (de) * 1990-11-22 1992-05-27 Boehringer Mannheim Gmbh Verfahren zur reaktivierung von denaturiertem protein
DE4113750A1 (de) * 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen
DE4139000A1 (de) * 1991-11-27 1993-06-03 Boehringer Mannheim Gmbh Verfahren zur gentechnologischen herstellung von biologisch aktivem ss-ngf
US5212091A (en) * 1992-03-02 1993-05-18 Monsanto Company Method of producing tissue factor pathway inhibitor
AU3812993A (en) * 1992-03-24 1993-10-21 Synergen, Inc. Refolding and purification of insulin-like growth factor I
SG46612A1 (en) 1992-12-02 1998-02-20 Hoechst Ag Process for obtaining proinsulin in processing correctly linked cystine bridges
DE4405179A1 (de) * 1994-02-18 1995-08-24 Hoechst Ag Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
FR2729972B1 (fr) * 1995-01-31 1997-04-18 Sanofi Sa Procede d'extraction de proteines periplasmiques de microorganismes procaryotes en presence d'arginine
US5714371A (en) * 1995-05-12 1998-02-03 Schering Corporation Method for refolding insoluble aggregates of hepatitis C virus protease
US5728804A (en) * 1995-06-02 1998-03-17 Research Corporation Technologies, Inc. Use of cyclodextrins for protein renaturation
AU714318B2 (en) * 1996-06-11 2000-01-06 Roche Diagnostics Gmbh Process for the activation of denatured protein
US7153943B2 (en) 1997-07-14 2006-12-26 Bolder Biotechnology, Inc. Derivatives of growth hormone and related proteins, and methods of use thereof
US6653098B1 (en) * 1998-02-23 2003-11-25 G. D. Searle & Co. Method of producing mouse and human endostatin
DE19850429A1 (de) * 1998-10-27 2000-05-04 Andre Schrattenholz Fragmente
EP1048732A1 (de) * 1999-04-26 2000-11-02 F. Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen
EP1077263A1 (de) * 1999-07-29 2001-02-21 F.Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen durch Co-Sekretion von Chaperonen
NZ522847A (en) 2000-05-16 2004-11-26 Bolder Biotechnology Inc Methods for refolding proteins containing free cysteine residues
DE10105911A1 (de) 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Expression der rekombinanten Proteinase K aus Tritirachium album in Hefe
DE10105912A1 (de) * 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Rekombinante Proteinase K
DE102005033250A1 (de) 2005-07-15 2007-01-18 Bioceuticals Arzneimittel Ag Verfahren zur Reinigung von G-CSF
DE102006009437A1 (de) 2006-03-01 2007-09-13 Bioceuticals Arzneimittel Ag G-CSF-Flüssigformulierung
CN101506222B (zh) 2006-07-14 2013-10-02 健泰科生物技术公司 重组蛋白的重折叠
US8617531B2 (en) 2006-12-14 2013-12-31 Bolder Biotechnology, Inc. Methods of making proteins and peptides containing a single free cysteine
BR112012022518A2 (pt) 2010-03-17 2020-10-13 Biogenerix Gmbh método para obtenção de g- csf recombinante humano biologicamente ativo
CA2815342A1 (en) * 2010-10-20 2012-04-26 Medimmune, Llc Methods for processing inclusion bodies
HUP1200171A1 (hu) 2012-03-19 2013-09-30 Richter Gedeon Nyrt Módszerek polipeptidek elõállítására
HUP1200172A2 (en) 2012-03-19 2013-10-28 Richter Gedeon Nyrt Methods for refolding g-csf from inclusion bodies
CN103852527B (zh) * 2012-12-05 2015-05-13 中国科学院大连化学物理研究所 一种高通量蛋白质样品预处理装置
US10457716B2 (en) 2014-08-06 2019-10-29 University Of Notre Dame Du Lac Protein folding and methods of using same
US10919948B2 (en) 2016-12-30 2021-02-16 Biogend Therapeutics Co., Ltd. Recombinant polypeptides, compositions, and methods thereof
SMT202500333T1 (it) 2020-12-18 2025-11-10 Richter Gedeon Nyrt Metodi per la purificazione di proteina di fusione fc-peptide

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US4468633A (en) 1982-04-28 1984-08-28 The Bendix Corporation Adjustable microwave power combiner for a plurality of coaxially mounted impatt diodes
IL68561A (en) 1982-05-05 1991-01-31 Genentech Inc Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof
US4432895A (en) * 1982-11-24 1984-02-21 Hoffmann-La Roche Inc. Monomeric interferons
GR79124B (da) * 1982-12-22 1984-10-02 Genentech Inc
DE122080T1 (de) * 1983-03-25 1985-05-23 Celltech Ltd., Slough, Berkshire Verfahren zur herstellung eines proteins.
US4530787A (en) * 1984-03-28 1985-07-23 Cetus Corporation Controlled oxidation of microbially produced cysteine-containing proteins
US4748234A (en) * 1985-06-26 1988-05-31 Cetus Corporation Process for recovering refractile bodies containing heterologous proteins from microbial hosts
US4766205A (en) * 1985-11-13 1988-08-23 Beatrice Companies, Inc. Method for isolation of recombinant polypeptides in biologically active forms
FR2596360B1 (fr) * 1986-04-01 1989-02-17 Sotralentz Sa Conteneur sur palette avec dispositif de protection en treillis plie et renforce
JPH0651119A (ja) * 1992-07-28 1994-02-25 Sekisui Chem Co Ltd 位相差板の製造方法

Also Published As

Publication number Publication date
FI933868A0 (fi) 1993-09-03
SU1607689A3 (ru) 1990-11-15
EP0253823A1 (de) 1988-01-27
PT83609A (de) 1986-11-01
DK200001897A (da) 2000-12-18
UA6023A1 (uk) 1994-12-29
ZA868012B (en) 1987-06-24
AU4132189A (en) 1990-01-04
HUT43643A (en) 1987-11-30
WO1987002673A3 (en) 1987-10-22
HRP921075B1 (en) 1999-02-28
JPH0824594B2 (ja) 1996-03-13
DE3537708A1 (de) 1987-04-23
FI872753A0 (fi) 1987-06-22
FI933868L (fi) 1993-09-03
HK153596A (en) 1996-08-16
EP0393725A1 (de) 1990-10-24
ES2020498T3 (es) 1996-04-01
FI94050C (fi) 1995-07-10
SK278317B6 (en) 1996-10-02
GR920300062T1 (en) 1992-08-31
JPH0728745B2 (ja) 1995-04-05
IE62634B1 (en) 1995-02-22
YU47185B (sh) 1995-01-31
EP0219874B1 (de) 1993-12-15
KR900009139B1 (ko) 1990-12-22
DK175109B1 (da) 2004-06-07
IL80325A0 (en) 1987-01-30
CA1329157C (en) 1994-05-03
DE3650449D1 (de) 1996-01-25
DE3689404D1 (de) 1994-01-27
DK175091B1 (da) 2004-05-24
CZ752686A3 (en) 1996-01-17
DE3537708C2 (da) 1993-07-08
HU204855B (en) 1992-02-28
CZ280727B6 (cs) 1996-04-17
FI94050B (fi) 1995-03-31
ATE98648T1 (de) 1994-01-15
EP0219874A3 (en) 1988-02-10
JPS62502895A (ja) 1987-11-19
ATE131489T1 (de) 1995-12-15
AU607083B2 (en) 1991-02-21
KR870700601A (ko) 1987-12-30
IL80325A (en) 1992-06-21
SI8611796A (sl) 1996-10-31
GR3018410T3 (en) 1996-03-31
YU179686A (en) 1988-06-30
FI872753L (fi) 1987-06-22
PT83609B (pt) 1988-10-14
JPH04218387A (ja) 1992-08-07
DD260517A5 (de) 1988-09-28
IE862683L (en) 1987-04-23
AU590029B2 (en) 1989-10-26
SI8611796B (sl) 1998-06-30
WO1987002673A2 (en) 1987-05-07
ES2061434T3 (es) 1994-12-16
AU6599386A (en) 1987-05-19
ES2020498A4 (es) 1991-08-16
DK320387A (da) 1987-06-23
SK752686A3 (en) 1996-10-02
EP0219874A2 (de) 1987-04-29
HRP921075A2 (en) 1995-06-30
FI95578B (fi) 1995-11-15
FI95578C (fi) 1996-02-26
HK153496A (en) 1996-08-16
EP0393725B1 (de) 1995-12-13

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