ES2061434T3 - Procedimiento para la activacion de proteinas eucarioticas heterologas que contienen puentes de disulfuro, preparadas mediante tecnologia genetica, despues de su expresion en procariotas. - Google Patents

Procedimiento para la activacion de proteinas eucarioticas heterologas que contienen puentes de disulfuro, preparadas mediante tecnologia genetica, despues de su expresion en procariotas.

Info

Publication number
ES2061434T3
ES2061434T3 ES86114731T ES86114731T ES2061434T3 ES 2061434 T3 ES2061434 T3 ES 2061434T3 ES 86114731 T ES86114731 T ES 86114731T ES 86114731 T ES86114731 T ES 86114731T ES 2061434 T3 ES2061434 T3 ES 2061434T3
Authority
ES
Spain
Prior art keywords
activation
denaturalizing
concentration
expression
procedure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES86114731T
Other languages
English (en)
Inventor
Rainer Dr Rudolph
Stephan Dr Fischer
Ralf Dr Mattes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2061434T3 publication Critical patent/ES2061434T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Lubricants (AREA)

Abstract

PARA LA ACTIVACION DE PROTEINAS EUCARIONTICAS, HETEROLOGAS, PREPARADAS POR TECNOLOGIA GENETICA, QUE CONTIENEN PUENTES DISULFURO POR EXPRESION EN PROCARIONTES MEDIANTE DISGREGACION CELULAR, SOLUBILIZACION EN CONDICIONES DESNATURALIZANTES Y REDUCTORAS Y ACTIVACION EN CONDICIONES OXIDANTES EN PRESENCIA DE GSH/GSSG NO SE TRABAJA EN LA ETAPA DE LA ACTIVACION A UN VALOR DE PH DE 9 A 12, CONCENTRACION DE GSH DE 0,1 A 20 MMOL/L, UNA CONCENTRACION DE 0,01 A 3 MMOL/L Y CONCENTRACION NO DESNATURALIZANTE DEL MEDIO DESNATURALIZANTE O EN LA QUE SE SEPARA EL MEDIO DE REDUCCION/DESNATURALIZACION, POR ADICION DE GSSG EN CONDICIONES DESNATURALIZANTES DEL GRUPO TIOL DE LA PROTEINA EN LA MEZCLA DE DISULFUROS DE PROTEINA Y GLUTATION. SE EFECTUA EN LA ETAPA DE LA ACTIVACION A PH DE 7 A 10,5, CONCENTRACION DE GSH DE 0,5 A 5MMOL/L Y A UNA CONCENTRACION NO DESNATURALIZANTE DEL AGENTE DE DESNATURALIZACION. EL PROCEDIMIENTO ES ESPECIALMENTE VALIOSO PARA T-PA E INTERFERON B.
ES86114731T 1985-10-23 1986-10-23 Procedimiento para la activacion de proteinas eucarioticas heterologas que contienen puentes de disulfuro, preparadas mediante tecnologia genetica, despues de su expresion en procariotas. Expired - Lifetime ES2061434T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19853537708 DE3537708A1 (de) 1985-10-23 1985-10-23 Verfahren zur aktivierung von t-pa nach expression in prokaryonten

Publications (1)

Publication Number Publication Date
ES2061434T3 true ES2061434T3 (es) 1994-12-16

Family

ID=6284269

Family Applications (2)

Application Number Title Priority Date Filing Date
ES90109721T Expired - Lifetime ES2020498T3 (es) 1985-10-23 1986-10-23 Procedimiento para la activacion de proteinas eucarioticas heterologas, que tienen puentes de disulfuro, preparadas por tecnologia genetica, despues de su expresion en el seno de procariotas.
ES86114731T Expired - Lifetime ES2061434T3 (es) 1985-10-23 1986-10-23 Procedimiento para la activacion de proteinas eucarioticas heterologas que contienen puentes de disulfuro, preparadas mediante tecnologia genetica, despues de su expresion en procariotas.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES90109721T Expired - Lifetime ES2020498T3 (es) 1985-10-23 1986-10-23 Procedimiento para la activacion de proteinas eucarioticas heterologas, que tienen puentes de disulfuro, preparadas por tecnologia genetica, despues de su expresion en el seno de procariotas.

Country Status (26)

Country Link
EP (3) EP0219874B1 (es)
JP (2) JPH0728745B2 (es)
KR (1) KR900009139B1 (es)
AT (2) ATE131489T1 (es)
AU (2) AU590029B2 (es)
CA (1) CA1329157C (es)
CZ (1) CZ280727B6 (es)
DD (1) DD260517A5 (es)
DE (3) DE3537708A1 (es)
DK (2) DK175091B1 (es)
ES (2) ES2020498T3 (es)
FI (2) FI94050C (es)
GR (2) GR920300062T1 (es)
HK (2) HK153596A (es)
HR (1) HRP921075B1 (es)
HU (2) HU204855B (es)
IE (1) IE62634B1 (es)
IL (1) IL80325A (es)
PT (1) PT83609B (es)
SI (1) SI8611796B (es)
SK (1) SK278317B6 (es)
SU (1) SU1607689A3 (es)
UA (1) UA6023A1 (es)
WO (1) WO1987002673A2 (es)
YU (1) YU47185B (es)
ZA (1) ZA868012B (es)

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JP2581668B2 (ja) * 1985-11-27 1997-02-12 三井東圧化学株式会社 ヒト正常細胞由来のヒト組織プラスミノ−ゲン活性化因子をコ−ドする新しいｄｎａ配列とそれを含むベクタ−及び細胞
US4777043A (en) * 1985-12-17 1988-10-11 Genentech, Inc. Stabilized human tissue plasminogen activator compositions
AU621051B2 (en) * 1987-04-28 1992-03-05 Amgen, Inc. Method for purifying granulocyte-macrophage colony stimulating factor
DE3722082A1 (de) * 1987-07-03 1989-01-12 Behringwerke Ag Verfahren zur bestimmung der aktivitaet von serinproteasen oder serinproteaseinhibitoren
CA1340586C (en) * 1988-09-23 1999-06-08 Cetus Corporation Process for recovering microbially produced interferon-beta
DE3832898A1 (de) * 1988-09-28 1990-04-12 Boehringer Mannheim Gmbh Praeparat von in prokaryonten exprimiertem plasminogenaktivator
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
DE3903581A1 (de) * 1989-02-07 1990-08-16 Boehringer Mannheim Gmbh Gewebs-plasminogenaktivator-derivat
DE3942143A1 (de) * 1989-12-20 1991-06-27 Boehringer Mannheim Gmbh T-pa pro stabilisierung
AU8417091A (en) * 1990-08-20 1992-03-17 Novo Nordisk A/S Biologically active compound, a process for the preparation thereof and use of the same
AU664021B2 (en) * 1990-09-05 1995-11-02 Natinco Nv Solubilization of proteins in active forms
DE4037196A1 (de) * 1990-11-22 1992-05-27 Boehringer Mannheim Gmbh Verfahren zur reaktivierung von denaturiertem protein
DE4113750A1 (de) * 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen
DE4139000A1 (de) * 1991-11-27 1993-06-03 Boehringer Mannheim Gmbh Verfahren zur gentechnologischen herstellung von biologisch aktivem ss-ngf
US5212091A (en) * 1992-03-02 1993-05-18 Monsanto Company Method of producing tissue factor pathway inhibitor
WO1993019084A1 (en) * 1992-03-24 1993-09-30 Synergen, Inc. Refolding and purification of insulin-like growth factor i
DK0600372T3 (da) 1992-12-02 1997-08-11 Hoechst Ag Fremgangsmåde til fremstilling af proinsulin med korrekt forbundne cystinbroer.
DE4405179A1 (de) * 1994-02-18 1995-08-24 Hoechst Ag Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
FR2729972B1 (fr) * 1995-01-31 1997-04-18 Sanofi Sa Procede d'extraction de proteines periplasmiques de microorganismes procaryotes en presence d'arginine
US5714371A (en) * 1995-05-12 1998-02-03 Schering Corporation Method for refolding insoluble aggregates of hepatitis C virus protease
US5728804A (en) * 1995-06-02 1998-03-17 Research Corporation Technologies, Inc. Use of cyclodextrins for protein renaturation
US6342585B1 (en) * 1996-06-11 2002-01-29 Roche Diagnostics Gmbh Method of activating denatured protein
US7153943B2 (en) 1997-07-14 2006-12-26 Bolder Biotechnology, Inc. Derivatives of growth hormone and related proteins, and methods of use thereof
US6653098B1 (en) 1998-02-23 2003-11-25 G. D. Searle & Co. Method of producing mouse and human endostatin
DE19850429A1 (de) * 1998-10-27 2000-05-04 Andre Schrattenholz Fragmente
EP1048732A1 (de) * 1999-04-26 2000-11-02 F. Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen
EP1077263A1 (de) 1999-07-29 2001-02-21 F.Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen durch Co-Sekretion von Chaperonen
IL152804A0 (en) 2000-05-16 2003-06-24 Bolder Biotechnology Inc Methods for refolding proteins containing free cysteine residues
DE10105912A1 (de) * 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Rekombinante Proteinase K
DE10105911A1 (de) 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Expression der rekombinanten Proteinase K aus Tritirachium album in Hefe
DE102005033250A1 (de) 2005-07-15 2007-01-18 Bioceuticals Arzneimittel Ag Verfahren zur Reinigung von G-CSF
DE202006020194U1 (de) 2006-03-01 2007-12-06 Bioceuticals Arzneimittel Ag G-CSF-Flüssigformulierung
US20080125580A1 (en) 2006-07-14 2008-05-29 Genentech, Inc. Refolding of Recombinant Proteins
US8617531B2 (en) 2006-12-14 2013-12-31 Bolder Biotechnology, Inc. Methods of making proteins and peptides containing a single free cysteine
MX2012010661A (es) 2010-03-17 2013-03-25 Biogenerix Gmbh Metodo para obtener factor estimulante de colonias de granulocitos (g-csf) humano recombinante biologicamente activo.
AU2011317021B2 (en) * 2010-10-20 2015-07-09 Medimmune, Llc Methods for processing inclusion bodies
HUP1200171A1 (hu) 2012-03-19 2013-09-30 Richter Gedeon Nyrt Módszerek polipeptidek elõállítására
HUP1200172A2 (en) 2012-03-19 2013-10-28 Richter Gedeon Nyrt Methods for refolding g-csf from inclusion bodies
CN103852527B (zh) * 2012-12-05 2015-05-13 中国科学院大连化学物理研究所 一种高通量蛋白质样品预处理装置
US10457716B2 (en) 2014-08-06 2019-10-29 University Of Notre Dame Du Lac Protein folding and methods of using same
CA3044371C (en) 2016-12-30 2024-01-16 Biogend Therapeutics Co., Ltd. Recombinant polypeptides and nucleic acid molecules, compositions, and methods of making and uses thereof
PL4263568T3 (pl) 2020-12-18 2025-11-24 Richter Gedeon Nyrt. Sposoby oczyszczania poddanego ponownemu fałdowaniu białka fuzyjnego peptydu Fc

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US4468633A (en) 1982-04-28 1984-08-28 The Bendix Corporation Adjustable microwave power combiner for a plurality of coaxially mounted impatt diodes
IL68561A (en) 1982-05-05 1991-01-31 Genentech Inc Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof
US4432895A (en) * 1982-11-24 1984-02-21 Hoffmann-La Roche Inc. Monomeric interferons
GR79124B (es) * 1982-12-22 1984-10-02 Genentech Inc
WO1984003711A1 (en) * 1983-03-25 1984-09-27 Celltech Ltd A process for the production of a protein
US4530787A (en) * 1984-03-28 1985-07-23 Cetus Corporation Controlled oxidation of microbially produced cysteine-containing proteins
US4748234A (en) * 1985-06-26 1988-05-31 Cetus Corporation Process for recovering refractile bodies containing heterologous proteins from microbial hosts
US4766205A (en) * 1985-11-13 1988-08-23 Beatrice Companies, Inc. Method for isolation of recombinant polypeptides in biologically active forms
FR2596360B1 (fr) * 1986-04-01 1989-02-17 Sotralentz Sa Conteneur sur palette avec dispositif de protection en treillis plie et renforce
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Also Published As

Publication number Publication date
AU4132189A (en) 1990-01-04
FI95578B (fi) 1995-11-15
FI95578C (fi) 1996-02-26
SK752686A3 (en) 1996-10-02
DK175109B1 (da) 2004-06-07
SI8611796B (sl) 1998-06-30
YU179686A (en) 1988-06-30
IL80325A0 (en) 1987-01-30
DE3689404D1 (de) 1994-01-27
HK153496A (en) 1996-08-16
JPH0728745B2 (ja) 1995-04-05
DD260517A5 (de) 1988-09-28
ES2020498A4 (es) 1991-08-16
FI933868A0 (fi) 1993-09-03
DK320387A (da) 1987-06-23
JPH0824594B2 (ja) 1996-03-13
ES2020498T3 (es) 1996-04-01
CA1329157C (en) 1994-05-03
EP0253823A1 (de) 1988-01-27
SK278317B6 (en) 1996-10-02
WO1987002673A3 (en) 1987-10-22
UA6023A1 (uk) 1994-12-29
EP0219874B1 (de) 1993-12-15
HK153596A (en) 1996-08-16
FI872753A0 (fi) 1987-06-22
EP0219874A3 (en) 1988-02-10
JPS62502895A (ja) 1987-11-19
KR870700601A (ko) 1987-12-30
AU590029B2 (en) 1989-10-26
ATE131489T1 (de) 1995-12-15
HRP921075A2 (en) 1995-06-30
WO1987002673A2 (en) 1987-05-07
EP0219874A2 (de) 1987-04-29
IE862683L (en) 1987-04-23
FI933868L (fi) 1993-09-03
HUT43643A (en) 1987-11-30
IE62634B1 (en) 1995-02-22
GR3018410T3 (en) 1996-03-31
DE3650449D1 (de) 1996-01-25
ATE98648T1 (de) 1994-01-15
DK175091B1 (da) 2004-05-24
CZ752686A3 (en) 1996-01-17
DE3537708C2 (es) 1993-07-08
EP0393725A1 (de) 1990-10-24
PT83609B (pt) 1988-10-14
KR900009139B1 (ko) 1990-12-22
SU1607689A3 (ru) 1990-11-15
FI872753L (fi) 1987-06-22
DK320387D0 (da) 1987-06-23
SI8611796A (sl) 1996-10-31
DK200001897A (da) 2000-12-18
FI94050C (fi) 1995-07-10
AU6599386A (en) 1987-05-19
DE3537708A1 (de) 1987-04-23
PT83609A (de) 1986-11-01
HRP921075B1 (en) 1999-02-28
HU204855B (en) 1992-02-28
CZ280727B6 (cs) 1996-04-17
ZA868012B (en) 1987-06-24
YU47185B (sh) 1995-01-31
AU607083B2 (en) 1991-02-21
IL80325A (en) 1992-06-21
GR920300062T1 (en) 1992-08-31
FI94050B (fi) 1995-03-31
EP0393725B1 (de) 1995-12-13
JPH04218387A (ja) 1992-08-07

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