DK320387A - Fremgangsmaade til aktivering af genteknologisk fremstillede, heterologe, disulfidbroer indeholdende eukaryotiske proteiner efter ekspression i prokaryotiske celler - Google Patents

Fremgangsmaade til aktivering af genteknologisk fremstillede, heterologe, disulfidbroer indeholdende eukaryotiske proteiner efter ekspression i prokaryotiske celler

Info

Publication number: DK320387A
Authority: DK; Denmark
Prior art keywords: expression; pref; activating; gentechnologically; heterologist
Prior art date: 1985-10-23

Application number

DK320387A

Other languages

English (en)

Other versions

DK175091B1 (da

DK320387D0 (da

Inventor

Stephan Fischer

Ralf Mattes

Rainer Rudolph

Original Assignee

Boehringer Mannheim Gmbh

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-10-23

Filing date

1987-06-23

Publication date

1987-06-23

1987-06-23 Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh

1987-06-23 Publication of DK320387A publication Critical patent/DK320387A/da

1987-06-23 Publication of DK320387D0 publication Critical patent/DK320387D0/da

2000-12-18 Priority to DK200001897A priority Critical patent/DK175109B1/da

2004-05-24 Application granted granted Critical

2004-05-24 Publication of DK175091B1 publication Critical patent/DK175091B1/da

Links

238000000034 method Methods 0.000 title abstract 3
230000003213 activating effect Effects 0.000 title abstract 2
230000014509 gene expression Effects 0.000 title abstract 2
102000004169 proteins and genes Human genes 0.000 title 1
108090000623 proteins and genes Proteins 0.000 title 1
102000003978 Tissue Plasminogen Activator Human genes 0.000 abstract 3
108090000373 Tissue Plasminogen Activator Proteins 0.000 abstract 3
229960000187 tissue plasminogen activator Drugs 0.000 abstract 3
239000003795 chemical substances by application Substances 0.000 abstract 2
239000004475 Arginine Substances 0.000 abstract 1
241000588724 Escherichia coli Species 0.000 abstract 1
ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 abstract 1
241000589776 Pseudomonas putida Species 0.000 abstract 1
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract 1
ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 abstract 1
239000004202 carbamide Substances 0.000 abstract 1
230000006037 cell lysis Effects 0.000 abstract 1
RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 abstract 1
YPZRWBKMTBYPTK-BJDJZHNGSA-N glutathione disulfide Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@H](C(=O)NCC(O)=O)CSSC[C@@H](C(=O)NCC(O)=O)NC(=O)CC[C@H](N)C(O)=O YPZRWBKMTBYPTK-BJDJZHNGSA-N 0.000 abstract 1
229960000789 guanidine hydrochloride Drugs 0.000 abstract 1
PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 abstract 1
210000001236 prokaryotic cell Anatomy 0.000 abstract 1
230000007420 reactivation Effects 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/565—IFN-beta
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Zoology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Medicinal Chemistry (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
General Engineering & Computer Science (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Biophysics (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Chemical Kinetics & Catalysis (AREA)
Gastroenterology & Hepatology (AREA)
Toxicology (AREA)
General Chemical & Material Sciences (AREA)
Analytical Chemistry (AREA)
Peptides Or Proteins (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Enzymes And Modification Thereof (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Mechanical Treatment Of Semiconductor (AREA)
Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Polysaccharides And Polysaccharide Derivatives (AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Lubricants (AREA)

DK198703203A 1985-10-23 1987-06-23 Fremgangsmåde til aktivering af genteknologisk fremstillede, heterologe, disulfidbro-holdige eukaryotiske proteiner efter ekspression i prokaryotiske celler DK175091B1 (da)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
DK200001897A DK175109B1 (da)	1985-10-23	2000-12-18	Fremgangsmåde til aktivering af genteknologisk fremstillede, heterologe, disulfidbroer indeholdende eukaryotiske proteiner efter ekspression i prokaryotiske celler

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date	Title
DE19853537708 DE3537708A1 (de)	1985-10-23	1985-10-23	Verfahren zur aktivierung von t-pa nach expression in prokaryonten
DE3537708		1985-10-23
EP8600610		1986-10-23
PCT/EP1986/000610 WO1987002673A2 (en)	1985-10-23	1986-10-23	Process for activating heterologous, eucaryotic proteins genetically engineered and presenting disulphide bridges after their expression in procaryotic cells

Publications (3)

Publication Number	Publication Date
DK320387A true DK320387A (da)	1987-06-23
DK320387D0 DK320387D0 (da)	1987-06-23
DK175091B1 DK175091B1 (da)	2004-05-24

Family

ID=6284269

Family Applications (2)

Application Number	Title	Priority Date	Filing Date
DK198703203A DK175091B1 (da)	1985-10-23	1987-06-23	Fremgangsmåde til aktivering af genteknologisk fremstillede, heterologe, disulfidbro-holdige eukaryotiske proteiner efter ekspression i prokaryotiske celler
DK200001897A DK175109B1 (da)	1985-10-23	2000-12-18	Fremgangsmåde til aktivering af genteknologisk fremstillede, heterologe, disulfidbroer indeholdende eukaryotiske proteiner efter ekspression i prokaryotiske celler

Family Applications After (1)

Application Number	Title	Priority Date	Filing Date
DK200001897A DK175109B1 (da)	1985-10-23	2000-12-18	Fremgangsmåde til aktivering af genteknologisk fremstillede, heterologe, disulfidbroer indeholdende eukaryotiske proteiner efter ekspression i prokaryotiske celler

Country Status (26)

Country	Link
EP (3)	EP0219874B1 (da)
JP (2)	JPH0728745B2 (da)
KR (1)	KR900009139B1 (da)
AT (2)	ATE98648T1 (da)
AU (2)	AU590029B2 (da)
CA (1)	CA1329157C (da)
CZ (1)	CZ280727B6 (da)
DD (1)	DD260517A5 (da)
DE (3)	DE3537708A1 (da)
DK (2)	DK175091B1 (da)
ES (2)	ES2061434T3 (da)
FI (2)	FI94050C (da)
GR (2)	GR920300062T1 (da)
HK (2)	HK153596A (da)
HR (1)	HRP921075B1 (da)
HU (2)	HU204855B (da)
IE (1)	IE62634B1 (da)
IL (1)	IL80325A (da)
PT (1)	PT83609B (da)
SI (1)	SI8611796B (da)
SK (1)	SK278317B6 (da)
SU (1)	SU1607689A3 (da)
UA (1)	UA6023A1 (da)
WO (1)	WO1987002673A2 (da)
YU (1)	YU47185B (da)
ZA (1)	ZA868012B (da)

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US4766205A (en) *	1985-11-13	1988-08-23	Beatrice Companies, Inc.	Method for isolation of recombinant polypeptides in biologically active forms
JP2581668B2 (ja) *	1985-11-27	1997-02-12	三井東圧化学株式会社	ヒト正常細胞由来のヒト組織プラスミノ−ゲン活性化因子をコ−ドする新しいｄｎａ配列とそれを含むベクタ−及び細胞
US4777043A (en) *	1985-12-17	1988-10-11	Genentech, Inc.	Stabilized human tissue plasminogen activator compositions
AU621051B2 (en) *	1987-04-28	1992-03-05	Amgen, Inc.	Method for purifying granulocyte-macrophage colony stimulating factor
DE3722082A1 (de) *	1987-07-03	1989-01-12	Behringwerke Ag	Verfahren zur bestimmung der aktivitaet von serinproteasen oder serinproteaseinhibitoren
CA1340586C (en) *	1988-09-23	1999-06-08	Cetus Corporation	Process for recovering microbially produced interferon-beta
DE3832898A1 (de) *	1988-09-28	1990-04-12	Boehringer Mannheim Gmbh	Praeparat von in prokaryonten exprimiertem plasminogenaktivator
DE3835350A1 (de) *	1988-10-17	1990-04-19	Boehringer Mannheim Gmbh	Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
DE3903581A1 (de) *	1989-02-07	1990-08-16	Boehringer Mannheim Gmbh	Gewebs-plasminogenaktivator-derivat
DE3942143A1 (de) *	1989-12-20	1991-06-27	Boehringer Mannheim Gmbh	T-pa pro stabilisierung
JPH06500084A (ja) *	1990-08-20	1994-01-06	ノボ　ノルディスク　アクティーゼルスカブ	生物学的に活性な化合物、その製造方法及びその利用
AU664021B2 (en) *	1990-09-05	1995-11-02	Natinco Nv	Solubilization of proteins in active forms
DE4037196A1 (de) *	1990-11-22	1992-05-27	Boehringer Mannheim Gmbh	Verfahren zur reaktivierung von denaturiertem protein
DE4113750A1 (de)	1991-04-26	1992-10-29	Boehringer Mannheim Gmbh	Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen
DE4139000A1 (de)	1991-11-27	1993-06-03	Boehringer Mannheim Gmbh	Verfahren zur gentechnologischen herstellung von biologisch aktivem ss-ngf
US5212091A (en) *	1992-03-02	1993-05-18	Monsanto Company	Method of producing tissue factor pathway inhibitor
WO1993019084A1 (en) *	1992-03-24	1993-09-30	Synergen, Inc.	Refolding and purification of insulin-like growth factor i
ATE148710T1 (de)	1992-12-02	1997-02-15	Hoechst Ag	Verfahren zur gewinnung von proinsulin mit korrekt verbundenen cystinbrücken
DE4405179A1 (de) *	1994-02-18	1995-08-24	Hoechst Ag	Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
FR2729972B1 (fr) *	1995-01-31	1997-04-18	Sanofi Sa	Procede d'extraction de proteines periplasmiques de microorganismes procaryotes en presence d'arginine
US5714371A (en) *	1995-05-12	1998-02-03	Schering Corporation	Method for refolding insoluble aggregates of hepatitis C virus protease
US5728804A (en) *	1995-06-02	1998-03-17	Research Corporation Technologies, Inc.	Use of cyclodextrins for protein renaturation
TR199802584T2 (xx) *	1996-06-11	1999-03-22	Boehringer Mannheim Gmbh	Denat�re proteinin aktive edilmesi i�in y�ntem.
US7153943B2 (en)	1997-07-14	2006-12-26	Bolder Biotechnology, Inc.	Derivatives of growth hormone and related proteins, and methods of use thereof
US6653098B1 (en)	1998-02-23	2003-11-25	G. D. Searle & Co.	Method of producing mouse and human endostatin
DE19850429A1 (de) *	1998-10-27	2000-05-04	Andre Schrattenholz	Fragmente
EP1048732A1 (de) *	1999-04-26	2000-11-02	F. Hoffmann-La Roche Ag	Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen
EP1077263A1 (de) *	1999-07-29	2001-02-21	F.Hoffmann-La Roche Ag	Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen durch Co-Sekretion von Chaperonen
IL152804A0 (en)	2000-05-16	2003-06-24	Bolder Biotechnology Inc	Methods for refolding proteins containing free cysteine residues
DE10105912A1 (de) *	2001-02-09	2002-08-14	Roche Diagnostics Gmbh	Rekombinante Proteinase K
DE10105911A1 (de)	2001-02-09	2002-08-14	Roche Diagnostics Gmbh	Expression der rekombinanten Proteinase K aus Tritirachium album in Hefe
DE102005033250A1 (de)	2005-07-15	2007-01-18	Bioceuticals Arzneimittel Ag	Verfahren zur Reinigung von G-CSF
DE202006020194U1 (de)	2006-03-01	2007-12-06	Bioceuticals Arzneimittel Ag	G-CSF-Flüssigformulierung
AR062069A1 (es)	2006-07-14	2008-10-15	Genentech Inc	Replegado de proteinas recombinantes
WO2008076933A2 (en)	2006-12-14	2008-06-26	Bolder Biotechnology, Inc.	Long acting proteins and peptides and methods of making and using the same
CN102892780B (zh)	2010-03-17	2015-07-29	通益制药有限公司	获得生物活性的重组人g-csf的方法
CN103168046A (zh) *	2010-10-20	2013-06-19	米迪缪尼有限公司	用于加工包涵体的方法
HUP1200172A2 (en)	2012-03-19	2013-10-28	Richter Gedeon Nyrt	Methods for refolding g-csf from inclusion bodies
HUP1200171A1 (hu)	2012-03-19	2013-09-30	Richter Gedeon Nyrt	Módszerek polipeptidek elõállítására
CN103852527B (zh) *	2012-12-05	2015-05-13	中国科学院大连化学物理研究所	一种高通量蛋白质样品预处理装置
US10457716B2 (en)	2014-08-06	2019-10-29	University Of Notre Dame Du Lac	Protein folding and methods of using same
NZ746109A (en)	2016-12-30	2022-12-23	Biogend Therapeutics Co Ltd	Recombinant polypeptides and nucleic acid molecules, compositions, and methods of making and uses thereof
ES3040791T3 (en)	2020-12-18	2025-11-05	Richter Gedeon Nyrt	Methods for the purification of refolded fc-peptide fusion protein

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US4468633A (en)	1982-04-28	1984-08-28	The Bendix Corporation	Adjustable microwave power combiner for a plurality of coaxially mounted impatt diodes
GR79202B (da)	1982-05-05	1984-10-22	Genentech Inc
US4432895A (en) *	1982-11-24	1984-02-21	Hoffmann-La Roche Inc.	Monomeric interferons
GR79124B (da)	1982-12-22	1984-10-02	Genentech Inc
EP0122080B1 (en) *	1983-03-25	1989-09-13	Celltech Limited	A process for the production of a protein
US4530787A (en) *	1984-03-28	1985-07-23	Cetus Corporation	Controlled oxidation of microbially produced cysteine-containing proteins
US4748234A (en) *	1985-06-26	1988-05-31	Cetus Corporation	Process for recovering refractile bodies containing heterologous proteins from microbial hosts
US4766205A (en) *	1985-11-13	1988-08-23	Beatrice Companies, Inc.	Method for isolation of recombinant polypeptides in biologically active forms
FR2596360B1 (fr) *	1986-04-01	1989-02-17	Sotralentz Sa	Conteneur sur palette avec dispositif de protection en treillis plie et renforce
JPH0651119A (ja) *	1992-07-28	1994-02-25	Sekisui Chem Co Ltd	位相差板の製造方法

1985
- 1985-10-23 DE DE19853537708 patent/DE3537708A1/de active Granted
1986
- 1986-10-10 IE IE268386A patent/IE62634B1/en not_active IP Right Cessation
- 1986-10-15 IL IL80325A patent/IL80325A/xx not_active IP Right Cessation
- 1986-10-17 SK SK7526-86A patent/SK278317B6/sk unknown
- 1986-10-17 CZ CS867526A patent/CZ280727B6/cs not_active IP Right Cessation
- 1986-10-21 SI SI8611796A patent/SI8611796B/sl unknown
- 1986-10-21 YU YU179686A patent/YU47185B/sh unknown
- 1986-10-22 CA CA000521121A patent/CA1329157C/en not_active Expired - Lifetime
- 1986-10-22 DD DD29546886A patent/DD260517A5/de not_active IP Right Cessation
- 1986-10-22 ZA ZA868012A patent/ZA868012B/xx unknown
- 1986-10-23 AT AT86114731T patent/ATE98648T1/de not_active IP Right Cessation
- 1986-10-23 AT AT90109721T patent/ATE131489T1/de not_active IP Right Cessation
- 1986-10-23 DE DE3650449T patent/DE3650449D1/de not_active Expired - Lifetime
- 1986-10-23 AU AU65993/86A patent/AU590029B2/en not_active Ceased
- 1986-10-23 EP EP86114731A patent/EP0219874B1/de not_active Expired - Lifetime
- 1986-10-23 UA UA4202987A patent/UA6023A1/uk unknown
- 1986-10-23 DE DE86114731T patent/DE3689404D1/de not_active Expired - Lifetime
- 1986-10-23 HU HU865290A patent/HU204855B/hu unknown
- 1986-10-23 JP JP61505882A patent/JPH0728745B2/ja not_active Expired - Lifetime
- 1986-10-23 ES ES86114731T patent/ES2061434T3/es not_active Expired - Lifetime
- 1986-10-23 ES ES90109721T patent/ES2020498T3/es not_active Expired - Lifetime
- 1986-10-23 EP EP86906320A patent/EP0253823A1/de active Pending
- 1986-10-23 EP EP90109721A patent/EP0393725B1/de not_active Expired - Lifetime
- 1986-10-23 HU HU865290A patent/HUT43643A/hu unknown
- 1986-10-23 KR KR1019870700536A patent/KR900009139B1/ko not_active Expired
- 1986-10-23 WO PCT/EP1986/000610 patent/WO1987002673A2/de not_active Ceased
- 1986-10-23 PT PT83609A patent/PT83609B/pt not_active IP Right Cessation
1987
- 1987-06-22 SU SU874202987Q patent/SU1607689A3/ru active
- 1987-06-22 FI FI872753A patent/FI94050C/fi not_active IP Right Cessation
- 1987-06-23 DK DK198703203A patent/DK175091B1/da not_active IP Right Cessation
1989
- 1989-09-13 AU AU41321/89A patent/AU607083B2/en not_active Expired
1991
- 1991-04-12 JP JP3079762A patent/JPH0824594B2/ja not_active Expired - Lifetime
1992
- 1992-08-31 GR GR92300062T patent/GR920300062T1/el unknown
- 1992-10-16 HR HRP-1796/86A patent/HRP921075B1/xx not_active IP Right Cessation
1993
- 1993-09-03 FI FI933868A patent/FI95578C/fi not_active IP Right Cessation
1995
- 1995-12-14 GR GR950403376T patent/GR3018410T3/el unknown
1996
- 1996-08-08 HK HK153596A patent/HK153596A/xx not_active IP Right Cessation
- 1996-08-08 HK HK153496A patent/HK153496A/xx not_active IP Right Cessation
2000
- 2000-12-18 DK DK200001897A patent/DK175109B1/da not_active IP Right Cessation

Also Published As

Publication number	Publication date
HUT43643A (en)	1987-11-30
JPH0728745B2 (ja)	1995-04-05
EP0393725A1 (de)	1990-10-24
CZ280727B6 (cs)	1996-04-17
ES2020498A4 (es)	1991-08-16
SI8611796A (sl)	1996-10-31
DK175091B1 (da)	2004-05-24
KR900009139B1 (ko)	1990-12-22
PT83609A (de)	1986-11-01
EP0253823A1 (de)	1988-01-27
DE3537708A1 (de)	1987-04-23
SU1607689A3 (ru)	1990-11-15
IE62634B1 (en)	1995-02-22
EP0393725B1 (de)	1995-12-13
AU6599386A (en)	1987-05-19
YU47185B (sh)	1995-01-31
ATE98648T1 (de)	1994-01-15
EP0219874B1 (de)	1993-12-15
KR870700601A (ko)	1987-12-30
FI872753A0 (fi)	1987-06-22
PT83609B (pt)	1988-10-14
FI94050B (fi)	1995-03-31
EP0219874A3 (en)	1988-02-10
FI95578B (fi)	1995-11-15
FI95578C (fi)	1996-02-26
FI872753L (fi)	1987-06-22
IE862683L (en)	1987-04-23
FI933868A0 (fi)	1993-09-03
HK153596A (en)	1996-08-16
ATE131489T1 (de)	1995-12-15
IL80325A0 (en)	1987-01-30
AU4132189A (en)	1990-01-04
ZA868012B (en)	1987-06-24
DK200001897A (da)	2000-12-18
HRP921075B1 (en)	1999-02-28
WO1987002673A2 (en)	1987-05-07
IL80325A (en)	1992-06-21
DE3689404D1 (de)	1994-01-27
JPH0824594B2 (ja)	1996-03-13
EP0219874A2 (de)	1987-04-29
ES2061434T3 (es)	1994-12-16
SK752686A3 (en)	1996-10-02
GR920300062T1 (en)	1992-08-31
FI94050C (fi)	1995-07-10
GR3018410T3 (en)	1996-03-31
DE3650449D1 (de)	1996-01-25
HK153496A (en)	1996-08-16
DK320387D0 (da)	1987-06-23
UA6023A1 (uk)	1994-12-29
JPS62502895A (ja)	1987-11-19
HRP921075A2 (en)	1995-06-30
JPH04218387A (ja)	1992-08-07
FI933868L (fi)	1993-09-03
YU179686A (en)	1988-06-30
ES2020498T3 (es)	1996-04-01
DK175109B1 (da)	2004-06-07
DD260517A5 (de)	1988-09-28
AU590029B2 (en)	1989-10-26
WO1987002673A3 (en)	1987-10-22
SI8611796B (sl)	1998-06-30
AU607083B2 (en)	1991-02-21
SK278317B6 (en)	1996-10-02
CA1329157C (en)	1994-05-03
HU204855B (en)	1992-02-28
CZ752686A3 (en)	1996-01-17
DE3537708C2 (da)	1993-07-08

Legal Events

Date	Code	Title	Description
2006-11-13	PUP	Patent expired

Publication	Publication Date	Title
DK320387A (da)	1987-06-23	Fremgangsmaade til aktivering af genteknologisk fremstillede, heterologe, disulfidbroer indeholdende eukaryotiske proteiner efter ekspression i prokaryotiske celler
Schwartz et al.	1977	Degradation of myofibrillar proteins by cathepsins B and D
ATE53762T1 (de)	1990-06-15	Fluessige diclofenac-zubereitungen.
DK623186D0 (da)	1986-12-22	Polypeptid med vaevsplasminogen-aktivator-virkning
DE3686654D1 (de)	1992-10-08	Verfahren zur isolierung rekombinanter polypeptide in biologisch aktiver form.
NO864990L (no)	1987-06-12	Solubilisering av proteinaggregater.
DK394786D0 (da)	1986-08-19	Fremgangsmaade til rensning og aktivering af proteiner fra uoploeselige inklusionslegemer
ES469328A1 (es)	1978-11-16	Procedimiento para el enriquecimiento de una nueva glucopre-teina.
DE3680271D1 (de)	1991-08-22	Thrombolytische zusammensetzung und verfahren zu deren herstellung.
Nagler et al.	1976	Subunit structure of bovine milk xanthine oxidase. Effect of limited cleavage by proteolytic enzymes on activity and structure
Takahashi et al.	1984	Thrombospondin fragmentation by α-thrombin and resistance to γ-thrombin
Duque-Magalhaes et al.	1980	Cytochrome c degrading activity in rat liver mitochondria
DK104390A (da)	1990-06-21	Vaevs-plasminogen aktivatorforbindelse, dna, der koder herfor, udtrykkelsesvektor indeholdende dette dna, vaertsceller transformeret med udtrykkelsesvektoren, fremgangsmaade til fremstilling af aktivatoren og farmaceutisk praeparat indeholdende denne
CHIEN	1977	Threshold Limit Value of Subtilising