DK81387A - Renfremstilling af proteiner - Google Patents

Renfremstilling af proteiner

Info

Publication number: DK81387A
Authority: DK; Denmark
Prior art keywords: recombinant; erythropoietin; biologically active; chromatographic procedures; elution
Prior art date: 1985-06-20

Application number

DK081387A

Other languages

English (en)

Other versions

DK175251B1 (da

DK81387D0 (da

Inventor

Lai Por-Hsiung

Thomas Wayne Strickland

Original Assignee

Kirin Amgen Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-06-20

Filing date

1987-02-18

Publication date

1987-02-18

1985-06-20 Priority claimed from US06/747,119 external-priority patent/US4667016A/en

1987-02-18 Application filed by Kirin Amgen Inc filed Critical Kirin Amgen Inc

1987-02-18 Publication of DK81387A publication Critical patent/DK81387A/da

1987-02-18 Publication of DK81387D0 publication Critical patent/DK81387D0/da

2004-07-19 Application granted granted Critical

2004-07-19 Publication of DK175251B1 publication Critical patent/DK175251B1/da

Links

102000004169 proteins and genes Human genes 0.000 title abstract 2
108090000623 proteins and genes Proteins 0.000 title abstract 2
238000004140 cleaning Methods 0.000 title 1
OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 abstract 4
102000003951 Erythropoietin Human genes 0.000 abstract 3
108090000394 Erythropoietin Proteins 0.000 abstract 3
229940105423 erythropoietin Drugs 0.000 abstract 3
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 2
238000010828 elution Methods 0.000 abstract 2
238000000034 method Methods 0.000 abstract 2
239000011347 resin Substances 0.000 abstract 2
229920005989 resin Polymers 0.000 abstract 2
BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 abstract 1
102100031939 Erythropoietin Human genes 0.000 abstract 1
102000003886 Glycoproteins Human genes 0.000 abstract 1
108090000288 Glycoproteins Proteins 0.000 abstract 1
108091005804 Peptidases Proteins 0.000 abstract 1
239000004365 Protease Substances 0.000 abstract 1
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 1
239000002253 acid Substances 0.000 abstract 1
238000005571 anion exchange chromatography Methods 0.000 abstract 1
230000015556 catabolic process Effects 0.000 abstract 1
210000004027 cell Anatomy 0.000 abstract 1
239000006143 cell culture medium Substances 0.000 abstract 1
239000000356 contaminant Substances 0.000 abstract 1
239000012531 culture fluid Substances 0.000 abstract 1
238000006731 degradation reaction Methods 0.000 abstract 1
238000002955 isolation Methods 0.000 abstract 1
210000004962 mammalian cell Anatomy 0.000 abstract 1
239000000463 material Substances 0.000 abstract 1
239000002609 medium Substances 0.000 abstract 1
230000000116 mitigating effect Effects 0.000 abstract 1
238000004366 reverse phase liquid chromatography Methods 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/505—Erythropoietin [EPO]
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Medicinal Chemistry (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
Biophysics (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Toxicology (AREA)
Zoology (AREA)
Gastroenterology & Hepatology (AREA)
Analytical Chemistry (AREA)
Peptides Or Proteins (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Detergent Compositions (AREA)
Treatment Of Liquids With Adsorbents In General (AREA)
Cephalosporin Compounds (AREA)

DK198700813A 1985-06-20 1987-02-18 Renfremstilling af erythropoietin DK175251B1 (da)

Applications Claiming Priority (6)

Application Number	Priority Date	Filing Date	Title
US06/747,119 US4667016A (en)	1985-06-20	1985-06-20	Erythropoietin purification
US74711985		1985-06-20
US87215286A	1986-06-13	1986-06-13
US87215286		1986-06-13
PCT/US1986/001342 WO1986007594A1 (en)	1985-06-20	1986-06-20	Protein purification
US8601342		1986-06-20

Publications (3)

Publication Number	Publication Date
DK81387A true DK81387A (da)	1987-02-18
DK81387D0 DK81387D0 (da)	1987-02-18
DK175251B1 DK175251B1 (da)	2004-07-19

Family

ID=27114696

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DK198700813A DK175251B1 (da)	1985-06-20	1987-02-18	Renfremstilling af erythropoietin

Country Status (14)

Country	Link
EP (1)	EP0228452B1 (da)
JP (1)	JPH0698019B2 (da)
KR (1)	KR930003665B1 (da)
AT (1)	ATE120208T1 (da)
AU (1)	AU606578B2 (da)
CA (1)	CA1297635C (da)
DE (1)	DE3650276T2 (da)
DK (1)	DK175251B1 (da)
ES (1)	ES8801381A1 (da)
GR (1)	GR861624B (da)
IE (1)	IE69343B1 (da)
IL (1)	IL79176A (da)
PT (1)	PT82811B (da)
WO (1)	WO1986007594A1 (da)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5179199A (en) *	1986-10-20	1993-01-12	Genzyme Corporation	Protein purification
IL118201A (en) *	1995-05-11	2004-12-15	Roche Diagnostics Gmbh	Preparation comprising a protein with human erythropoietin activity which is free of serum and non-recombinant mammalian protein and process for the preparation thereof
KR100374308B1 (ko) *	1995-07-27	2003-12-18	주식회사 엘지생명과학	재조합차이니스햄스터오바리세포로부터발현된수두바이러스의지피i당단백질의정제방법
JP2001520009A (ja) *	1997-10-16	2001-10-30	ユニバーシティ・オブ・ジョージア・リサーチ・ファウンデイション・インコーポレイテッド	鳥の導入遺伝子についてのマグナム特異的プロモーターを含むベクター
US6210924B1 (en)	1998-08-11	2001-04-03	Amgen Inc.	Overexpressing cyclin D 1 in a eukaryotic cell line
BR9917606A (pt) *	1998-11-06	2002-12-31	Bio Sidus S A	Procedimento para a purificação de eritropoetina humana recombinante a partir de sobrenadantes de cultivo de células e eritropoetina humana recombinante obtida com tal procedimento
DE60228460D1 (de) *	2002-12-13	2008-10-02	Bioceuticals Arzneimittel Ag	Verfahren zur Herstellung und Reinigung von Erythropoietin
US7423139B2 (en)	2004-01-20	2008-09-09	Insight Biopharmaceuticals Ltd.	High level expression of recombinant human erythropoietin having a modified 5′-UTR
DE102004027816A1 (de) *	2004-06-08	2006-01-05	Bioceuticals Arzneimittel Ag	Verfahren zur Reinigung von Erythropoietin
IL165484A0 (en)	2004-11-30	2006-01-15	Applied Research Systems	Production of proteins
EP1966232A1 (en) *	2005-12-23	2008-09-10	Novo Nordisk A/S	Purification of proteins using preparative reverse phase chromatography (rpc)
KR100900033B1 (ko) *	2007-11-21	2009-06-01	한국생명공학연구원	인간 에리스로포이에틴의 정제방법
DE102008002209A1 (de) *	2008-06-04	2009-12-10	Evonik Degussa Gmbh	Verfahren zur Aufreinigung von Erythropoietin
CN102164954B (zh)	2008-09-23	2018-04-06	弗·哈夫曼-拉罗切有限公司	促红细胞生成素的纯化
DE102008054716A1 (de)	2008-12-16	2010-06-17	Evonik Degussa Gmbh	Inprozesskontrolle in einem Verfahren zur Herstellung von EPO
AU2010241154A1 (en) *	2009-04-22	2011-10-27	Bayer Bioscience N.V.	Production of multi-antennary N-glycan structures in plants
EA022396B1 (ru)	2009-09-23	2015-12-30	Ратиофарм Гмбх	Способ очистки рекомбинантного эритропоэтина человека (epo), эритропоэтин, очищенный данным способом, и фармацевтические композиции, содержащие такой эритропоэтин
WO2012022688A1 (de)	2010-08-20	2012-02-23	Boehringer Ingelheim International Gmbh	Verfahren zur inaktivierung von proteasen in einer aus einer zellkultur gewonnen flüssigkeit durch ph änderung
WO2013002330A1 (ja) *	2011-06-29	2013-01-03	協和発酵キリン株式会社	たん白質の精製方法
EP3153522A1 (en)	2015-10-09	2017-04-12	Hetero Drugs Limited	Process for the purification of erythropoietin and darbepoetin alfa

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
IN150740B (da) *	1978-11-24	1982-12-04	Hoffmann La Roche
JPS5616496A (en) *	1979-07-20	1981-02-17	Sumitomo Chem Co Ltd	Recovery of erythropoietin
JPS5653696A (en) *	1979-10-09	1981-05-13	Ajinomoto Co Inc	Isolation of erythropoietin by adsorption
JPS57149228A (en) *	1981-03-11	1982-09-14	Ajinomoto Co Inc	Novel erythropoietin and its preparation
US4558005A (en) *	1982-09-13	1985-12-10	University Patents, Inc.	Monoclonal anti-erythropoietin
JPH0686480B2 (ja) *	1983-02-21	1994-11-02	雪印乳業株式会社	エリスロポエチン製造用モノクローナル抗体
NZ210501A (en) *	1983-12-13	1991-08-27	Kirin Amgen Inc	Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence
US4568488A (en) *	1984-01-11	1986-02-04	Lee Huang Sylvia	Reverse immunoaffinity chromatography purification method
US4677195A (en) *	1985-01-11	1987-06-30	Genetics Institute, Inc.	Method for the purification of erythropoietin and erythropoietin compositions

1986
- 1986-04-20 IL IL79176A patent/IL79176A/xx not_active IP Right Cessation
- 1986-06-18 IE IE162886A patent/IE69343B1/en not_active IP Right Cessation
- 1986-06-18 CA CA000511855A patent/CA1297635C/en not_active Expired - Lifetime
- 1986-06-19 ES ES556257A patent/ES8801381A1/es not_active Expired
- 1986-06-20 DE DE3650276T patent/DE3650276T2/de not_active Expired - Lifetime
- 1986-06-20 PT PT82811A patent/PT82811B/pt unknown
- 1986-06-20 AT AT86904556T patent/ATE120208T1/de not_active IP Right Cessation
- 1986-06-20 WO PCT/US1986/001342 patent/WO1986007594A1/en not_active Ceased
- 1986-06-20 EP EP86904556A patent/EP0228452B1/en not_active Expired - Lifetime
- 1986-06-20 AU AU61230/86A patent/AU606578B2/en not_active Expired
- 1986-06-20 GR GR861624A patent/GR861624B/el unknown
- 1986-06-20 JP JP61503570A patent/JPH0698019B2/ja not_active Expired - Lifetime
- 1986-10-04 KR KR1019860008331A patent/KR930003665B1/ko not_active Expired - Lifetime
1987
- 1987-02-18 DK DK198700813A patent/DK175251B1/da not_active IP Right Cessation

Also Published As

Publication number	Publication date
IL79176A0 (en)	1986-09-30
ES556257A0 (es)	1988-01-01
ES8801381A1 (es)	1988-01-01
DK175251B1 (da)	2004-07-19
KR880000464A (ko)	1988-03-26
EP0228452A4 (en)	1989-05-16
PT82811B (pt)	1988-04-21
ATE120208T1 (de)	1995-04-15
JPH0698019B2 (ja)	1994-12-07
EP0228452A1 (en)	1987-07-15
PT82811A (en)	1986-07-01
DE3650276T2 (de)	1995-07-27
CA1297635C (en)	1992-03-17
AU606578B2 (en)	1991-02-14
DK81387D0 (da)	1987-02-18
JPS63503352A (ja)	1988-12-08
IE69343B1 (en)	1996-09-04
IE861628L (en)	1986-12-20
WO1986007594A1 (en)	1986-12-31
IL79176A (en)	1992-06-21
DE3650276D1 (de)	1995-04-27
EP0228452B1 (en)	1995-03-22
GR861624B (en)	1986-10-21
KR930003665B1 (ko)	1993-05-08
AU6123086A (en)	1987-01-13

Legal Events

Date	Code	Title	Description
2006-07-10	PUP	Patent expired

Publication	Publication Date	Title
DK81387A (da)	1987-02-18	Renfremstilling af proteiner
RIDGE et al.	1971	Role of peroxidase when hydroxyproline-rich protein in plant cell walls is increased by ethylene
US6471500B1 (en)	2002-10-29	Process for producing erythropoietin containing no animal proteins
Zechel	1980	Isolation of polymerization‐competent cytoplasmic actin by affinity chromatography on immobilized DNAse I using formamide as eluant
DK506488A (da)	1989-03-12	Fremgangsmaade til dyrkning af rekombinante celler, vaertscelle til anvendelse ved fremgangsmaaden og kultur omfattende vaertscellen
AU7575487A (en)	1988-01-21	Method for the preparation of proteins with factor VIII activity by microbial host cells; expression, vectors, host cells, antibodies
DK97784A (da)	1984-08-29	Dna-segment
US4777242A (en)	1988-10-11	Purification of recombinant tumor necrosis factor
ATE24934T1 (de)	1987-01-15	Abtrennung von plasma-proteinen aus zellkultursystemen.
Rihn et al.	1984	A new purification procedure for Clostridium difficile enterotoxin
Nath et al.	1990	Separation of enzymes from Candida boidinii crude extract by continuous flow zone electrophoresis
Emöd et al.	1977	Five sepharose‐bound ligands for the chromatographic purification of Clostridium collagenase and clostripain
Iwai et al.	1983	Purification of four esterases from Aspergillus niger NRRL 337
Dimroth	1982	Purification of the sodium transport enzyme oxaloacetate decarboxylase by affinity chromatography on avidin sepharose
Kovacs et al.	1985	Purification of hydrogenase by fast protein liquid chromatography and by conventional separation techniques: a comparative study
Chan et al.	1985	Dipeptidyl-aminopeptidases and aminopeptidases in Dictyostelium discoideum
CA1328538C (en)	1994-04-12	Method of separating an organic solvent from a protein mixture
Le Meur et al.	1972	Purification and properties of seryl-tRNA synthetase from hen's liver
Seyedyazdani et al.	1975	Molecular nature of β-galactosidase from different tissues in two strains of the house mouse
Paberit et al.	1984	Isolation and properties of neutral metalloprotease from thermophilic bacteria Bacillus brevis.
AU7378087A (en)	1987-12-10	Interleukin-2 induced antineoplastic peptide
ITRM920385A1 (it)	1993-11-22	Procedimento per la purificazione di proteine da sistemi cellulari.
DK1025121T3 (da)	2011-11-28	Peptider, som er i stand til at hæmme interaktionen mellem preseniliner og peptidet beta-amyloid eller dets precursor
Devlin et al.	1982	Biochemical studies of high molecular weight complex lymphotoxin and evidence that it is involved in cytotoxic T-cell mediated lysis