ES2570382T3 - Composiciones y métodos para la producción de azúcares fermentables - Google Patents

Composiciones y métodos para la producción de azúcares fermentables

Info

Publication number
ES2570382T3
ES2570382T3 ES11838704T ES11838704T ES2570382T3 ES 2570382 T3 ES2570382 T3 ES 2570382T3 ES 11838704 T ES11838704 T ES 11838704T ES 11838704 T ES11838704 T ES 11838704T ES 2570382 T3 ES2570382 T3 ES 2570382T3
Authority
ES
Spain
Prior art keywords
cellobiose oxidizing
compositions
production
methods
fungal cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES11838704T
Other languages
English (en)
Inventor
Ish Kumar Dhawan
Dipnath Baidyaroy
Andrew Shaw
Oleh Tanchak
Christopher Hill
Chengsong Liu
Amala Chokshi
Brian R Scott
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Codexis Inc
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Application granted granted Critical
Publication of ES2570382T3 publication Critical patent/ES2570382T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/99—Oxidoreductases acting on the CH-OH group of donors (1.1) with other acceptors (1.1.99)
    • C12Y101/99018—Cellobiose oxidase (1.1.99.18)
    • C—CHEMISTRY; METALLURGY
    • C13—SUGAR INDUSTRY
    • C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
    • C13K1/00—Glucose; Glucose-containing syrups
    • C13K1/02—Glucose; Glucose-containing syrups obtained by saccharification of cellulosic materials
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Emergency Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Una célula fúngica de Myceliophthora thermophila que ha sido genéticamente modificada para reducir la cantidad de actividad enzimática oxidante de celobiosa endógena de dos o más enzimas oxidantes de celobiosa endógena que se producen por la célula fúngica, en la que la célula fúngica ha sido genéticamente modificada para delecionar al menos parcialmente los genes que codifican las dos o más enzimas oxidantes de celobiosa endógena, en la que la primera de las dos o más enzimas oxidantes de celobiosa comprende una secuencia de aminoácidos que es al menos aproximadamente el 90 % idéntica a SEQ ID NO: 6, y en la que la segunda de las dos o más enzimas oxidantes de celobiosa comprende una secuencia de aminoácidos que es al menos el 90 % idéntica a SEQ ID NO: 8.
ES11838704T 2010-11-02 2011-11-01 Composiciones y métodos para la producción de azúcares fermentables Active ES2570382T3 (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US40947210P 2010-11-02 2010-11-02
US40921710P 2010-11-02 2010-11-02
US40918610P 2010-11-02 2010-11-02
US40948010P 2010-11-02 2010-11-02
PCT/US2011/058842 WO2012061432A1 (en) 2010-11-02 2011-11-01 Compositions and methods for production of fermentable sugars

Publications (1)

Publication Number Publication Date
ES2570382T3 true ES2570382T3 (es) 2016-05-18

Family

ID=45997183

Family Applications (1)

Application Number Title Priority Date Filing Date
ES11838704T Active ES2570382T3 (es) 2010-11-02 2011-11-01 Composiciones y métodos para la producción de azúcares fermentables

Country Status (9)

Country Link
US (3) US8236551B2 (es)
EP (1) EP2635690B1 (es)
CN (1) CN103189516A (es)
BR (1) BR112013010812A2 (es)
CA (1) CA2815430C (es)
DK (1) DK2635690T3 (es)
ES (1) ES2570382T3 (es)
HU (1) HUE028981T2 (es)
WO (1) WO2012061432A1 (es)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9493806B2 (en) 2001-06-01 2016-11-15 Colorado State University Research Foundation Enzymatic biosensing systems
US9493805B2 (en) 2001-06-01 2016-11-15 Colorado State University Research Foundation Enzymatic biosensors with enhanced activity retention for detection of organic compounds
US9796998B2 (en) 2007-04-09 2017-10-24 Colorado State University Research Foundation Oxygenase-based biosensing systems for measurement of halogenated alkene concentrations
US9394555B2 (en) * 2010-08-12 2016-07-19 Novozymes, Inc. Compositions comprising a polypeptide having cellulolytic enhancing activity and a dioxy compound and uses thereof
US9458483B2 (en) 2010-08-12 2016-10-04 Novozymes, Inc. Compositions comprising a polypeptide having cellulolytic enhancing activity and a bicyclic compound and uses thereof
ES2623288T3 (es) 2010-08-20 2017-07-10 Codexis, Inc. Uso de proteínas de la familia de glicólido hidrolasa 61 en el procesamiento de celulosa
WO2012061382A1 (en) * 2010-11-02 2012-05-10 Codexis, Inc. Improved fungal strains
WO2012071471A2 (en) 2010-11-22 2012-05-31 Colorado State University Research Foundation Biosensing systems for measurement of lactose
US9434972B2 (en) * 2011-01-31 2016-09-06 Novozymes North America, Inc. Processes for enzymatic refining of pretreated cellulosic material for saccharification
WO2013003219A1 (en) 2011-06-30 2013-01-03 Codexis, Inc. Pentose fermentation by a recombinant microorganism
US9499853B2 (en) 2011-08-02 2016-11-22 Colorado State University Research Foundation Biosensing system with extended lifetime via cofactor recycling
DK2748317T3 (en) 2011-08-22 2017-07-17 Codexis Inc GH61 glycoside hydrolase protein variants and cofactors that enhance GH61 activity
WO2013029170A1 (en) * 2011-08-26 2013-03-07 Iogen Energy Corporation A process for producing cellulase mixtures from myceliophthora and related organisms
EP2760997A4 (en) 2011-09-30 2015-02-11 Codexis Inc MUSHROOM PROTEASE
WO2013159005A2 (en) * 2012-04-19 2013-10-24 Dyadic International (Usa) Ltd. A method of improving the activity of cellulase enzyme mixtures in the saccharification of (ligno)cellulosic material
US8980578B2 (en) 2012-06-11 2015-03-17 Codexis, Inc. Fungal beta-xylosidase variants
KR101535526B1 (ko) * 2012-06-26 2015-07-09 한국생명공학연구원 야생형보다 분비능이 증가된 gh61 융합 단백질
UA118174C2 (uk) * 2012-07-02 2018-12-10 Ксілеко, Інк. Спосіб обробки біомаси
DK2895599T3 (en) * 2012-09-11 2017-10-30 Univ Danmarks Tekniske Hitherto Unknown LACCASE FROM GANODERMA LUCIDUM, ABLE TO IMPROVE ENZYMATIC DEGRADATION OF LIGNOCELLULOLYTIC BIOMASS
BR112015011466A8 (pt) 2012-11-20 2018-05-02 Codexis Inc Célula hospedeira fúngica recombinante, constructo de ácido nucleico recombinante, sequência de polpeptídeo isolada, e, métodos para produzir um produto de fermentação e pelo menos um produto final a partir de pelo menos um substrato celulósico
MX374839B (es) * 2012-12-20 2025-03-06 Archer Daniels Midland Co Produccion de biocombustibles a partir de esteres de acidos carboxilicos bioderivados.
US9850512B2 (en) 2013-03-15 2017-12-26 The Research Foundation For The State University Of New York Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield
WO2015039962A1 (en) * 2013-09-20 2015-03-26 Novozymes A/S Enzymatic bleaching of paper pulp
FI128419B (en) * 2014-01-13 2020-04-30 Upm Kymmene Corp A method for separating lignocellulose particle fraction and lignin particle fraction
US9951363B2 (en) 2014-03-14 2018-04-24 The Research Foundation for the State University of New York College of Environmental Science and Forestry Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects
KR101539535B1 (ko) * 2014-03-31 2015-07-27 고려대학교 산학협력단 곰팡이 유래의 글리코시드 하이드로레이즈 ６１을 발현하는 미생물 및 이를 이용한 셀룰로스 분해 촉진 방법
DK3419992T3 (da) 2016-02-22 2021-02-15 Danisco Us Inc Svampesystem til proteinproduktion med højt udbytte
WO2017170919A1 (ja) * 2016-03-31 2017-10-05 東レ株式会社 キシロオリゴ糖の製造方法
WO2017190188A1 (en) * 2016-05-02 2017-11-09 Swinburne University Of Technology A method of obtaining useful material from plant biomass waste
ES2647322B1 (es) * 2016-05-20 2018-10-05 Abengoa Bioenergía Nuevas Tecnologías, S.A. Célula hospedadora Myceliophthora thermophila con actividad celulolítica mejorada y composiciones enzimáticas obtenidas por la misma
CN105949254B (zh) * 2016-06-06 2018-08-31 青岛农业大学 一种糖苷化合物及其制备方法
CN107328883A (zh) * 2017-09-05 2017-11-07 南京凯通粮食生化研究设计有限公司 一种鼠李糖生产企业快速准确测定鼠李糖含量的方法
GB2566516A (en) 2017-09-15 2019-03-20 Univ Oxford Innovation Ltd Electrochemical recognition and quantification of cytochrome c oxidase expression in bacteria
WO2019074828A1 (en) 2017-10-09 2019-04-18 Danisco Us Inc CELLOBIOSE DEHYDROGENASE VARIANTS AND METHODS OF USE
CN112912511A (zh) * 2018-10-24 2021-06-04 帝斯曼知识产权资产管理有限公司 用于碳水化合物材料酶促水解和糖发酵的方法
CN110669742A (zh) * 2019-11-04 2020-01-10 孙芳 一种提高有机磷农药降解漆酶活力的方法
CN110754292B (zh) * 2019-11-25 2021-08-17 广东省微生物研究所（广东省微生物分析检测中心） 卵孢小奥德蘑白色变种及其人工栽培方法
CN111320266B (zh) * 2020-02-28 2022-05-20 江苏大学 一种染料厌氧生物脱色体系及方法
US20230313257A1 (en) * 2020-06-05 2023-10-05 The Board Of Trustees Of The Leland Stanford Junior University Methods and Systems to Secrete Lignin-Modifying Enzymes and Uses Thereof
CN112625921B (zh) * 2020-12-29 2022-05-20 中国科学院成都生物研究所 一种用于处理高木质素含量废弃物的菌制剂
CN115044696B (zh) * 2022-05-07 2024-09-17 浙江大学 一种蔬菜菌核病的早期分子快速检测方法及其应用
CN115896049B (zh) * 2022-11-28 2024-02-02 中南林业科技大学 纤维二糖脱氢酶基因、载体、重组菌及它们的应用
CN120210404B (zh) * 2025-03-03 2025-11-28 山东省食品药品检验研究院 一种用于鉴定中药材白薇、白前、老瓜头和徐长卿的mnp标记位点、引物组、试剂盒及鉴定方法

Family Cites Families (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3953295A (en) 1971-10-20 1976-04-27 Mallinckrodt, Inc. Reagent formulations for glucose assay
US4461648A (en) 1980-07-11 1984-07-24 Patrick Foody Method for increasing the accessibility of cellulose in lignocellulosic materials, particularly hardwoods agricultural residues and the like
US4356196A (en) 1980-10-20 1982-10-26 Hultquist Joe H Process for treating alfalfa and other cellulosic agricultural crops
US4451648A (en) 1981-08-19 1984-05-29 The United States Of America As Represented By The Department Of Health And Human Services Process for the production of 2-β-D-ribofuranosylthiazole-4-carboxamide
US4600590A (en) 1981-10-14 1986-07-15 Colorado State University Research Foundation Method for increasing the reactivity and digestibility of cellulose with ammonia
US5037663A (en) 1981-10-14 1991-08-06 Colorado State University Research Foundation Process for increasing the reactivity of cellulose-containing materials
US4556430A (en) 1982-09-20 1985-12-03 Trustees Of Dartmouth College Process for hydrolysis of biomass
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5171592A (en) 1990-03-02 1992-12-15 Afex Corporation Biomass refining process
US5360732A (en) * 1992-03-04 1994-11-01 Genecor International, Inc. Production of Aspergillus niger catalase-R
US5576220A (en) 1993-02-19 1996-11-19 Arris Pharmaceutical Corporation Thin film HPMP matrix systems and methods for constructing and displaying ligands
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
JP3390015B2 (ja) 1995-03-25 2003-03-24 ローネ−ポウレンク ローディア アクチェン ゲゼルシャフト 活性化しているポリサッカライドを得るためのプロセス、このプロセスによって作られるポリサッカライドおよびそれらの使用方法
US5811381A (en) 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US6352857B1 (en) 1997-01-21 2002-03-05 Wisconsin Alumni Research Foundation Treatment of diabetes with synthetic beta cells
US5916780A (en) 1997-06-09 1999-06-29 Iogen Corporation Pretreatment process for conversion of cellulose to fuel ethanol
US6015703A (en) 1998-03-10 2000-01-18 Iogen Corporation Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase
US6176176B1 (en) 1998-04-30 2001-01-23 Board Of Trustees Operating Michigan State University Apparatus for treating cellulosic materials
ES2237159T3 (es) 1998-10-06 2005-07-16 Mark Aaron Emalfarb Sistema de transformacion en el campo de los hongos micoticos filamentosos en chrysosporium.
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
ES2166316B1 (es) 2000-02-24 2003-02-16 Ct Investig Energeticas Ciemat Procedimiento de produccion de etanol a partir de biomasa lignocelulosica utilizando una nueva levadura termotolerante.
ES2266265T3 (es) 2000-09-25 2007-03-01 Iogen Energy Corporation Metodo para la produccion de glucosa con una mezcla de celulosa que comprende una celulosa modificada.
US7049485B2 (en) 2000-10-20 2006-05-23 Board Of Trustees Of Michigan State University Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars
WO2003070940A1 (en) 2002-02-25 2003-08-28 Oji Paper Co., Ltd. Cellulose digesting enzyme gene and utilization of the gene
US20050084907A1 (en) 2002-03-01 2005-04-21 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
WO2006011899A1 (en) 2003-11-25 2006-02-02 L-3 Communications Security and Detection Systems Corporation Security system for detecting nuclear masses
US20060004699A1 (en) 2004-06-30 2006-01-05 Nokia Corporation Method and system for managing metadata
DK176540B1 (da) 2004-09-24 2008-07-21 Cambi Bioethanol Aps Fremgangsmåde til behandling af biomasse og organisk affald med henblik på at udvinde önskede biologisk baserede produkter
US7727746B2 (en) 2004-12-17 2010-06-01 Iogen Energy Corporation Upflow reactor for enzymatic hydrolysis of cellulose
EP1869194A2 (en) 2005-04-12 2007-12-26 E.I.Du pont de nemours and company Treatment of biomass to obtain a target chemical
AU2006254627A1 (en) 2005-06-03 2006-12-07 Iogen Energy Corporation Method of continuous processing of lignocellulosic feedstocks
CA2655645A1 (en) 2006-06-22 2007-12-27 Iogen Energy Corporation Enzyme compositions for the improved enzymatic hydrolysis of cellulose and methods of using same
US8017373B2 (en) 2006-08-31 2011-09-13 Iogen Energy Corporation Process for enzymatic hydrolysis of pretreated lignocellulosic feedstocks
EP2505651A3 (en) 2006-12-10 2013-01-09 Dyadic International, Inc. Isolated fungus with reduced protease activity
US20080295980A1 (en) 2007-05-31 2008-12-04 Lignol Innovations Ltd. Continuous counter-current organosolv processing of lignocellulosic feedstocks
US7807419B2 (en) 2007-08-22 2010-10-05 E. I. Du Pont De Nemours And Company Process for concentrated biomass saccharification
DK2188381T3 (da) 2007-08-30 2012-04-02 Iogen Energy Corp Enzymatisk hydrolyse af lignocellulosisk råmateriale med anvendelse af accessoriske enzymer
CA2736661A1 (en) * 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes
US20090312196A1 (en) 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
US8328947B2 (en) 2008-08-29 2012-12-11 Iogen Energy Corporation Method for low water hydrolysis or pretreatment of polysaccharides in a lignocellulosic feedstock
CN102325889A (zh) * 2008-12-19 2012-01-18 诺维信股份有限公司 增加纤维素材料水解的方法
CN102325879A (zh) 2008-12-19 2012-01-18 诺维信股份有限公司 在纤维二糖脱氢酶存在下增加纤维素材料水解的方法
EP3421597B1 (en) 2009-03-16 2020-10-28 Danisco US Inc. Chrysosporium lucknowense protein production system
US8486683B2 (en) 2009-05-29 2013-07-16 Iogen Energy Corporation Beta-glucosidase enzymes
RU2012126131A (ru) * 2009-11-25 2013-12-27 Кодексис, Инк. РЕКОМБИНАНТНЫЕ ВАРИАНТЫ β-ГЛЮКОЗИДАЗЫ ДЛЯ ПРОДУКЦИИ РАСТВОРИМЫХ САХАРОВ ИЗ ЦЕЛЛЮЛОЗНОЙ БИОМАССЫ

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WO2012061432A1 (en) 2012-05-10
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