ECSP941123A - Genes que codifican el complejo cetoacido deshidrogenasa de cadena ramificada de streptomyces avermitilis - Google Patents

Genes que codifican el complejo cetoacido deshidrogenasa de cadena ramificada de streptomyces avermitilis

Info

Publication number
ECSP941123A
ECSP941123A EC1994001123A ECSP941123A ECSP941123A EC SP941123 A ECSP941123 A EC SP941123A EC 1994001123 A EC1994001123 A EC 1994001123A EC SP941123 A ECSP941123 A EC SP941123A EC SP941123 A ECSP941123 A EC SP941123A
Authority
EC
Ecuador
Prior art keywords
dehydrogenase complex
ketoacide
streptomyces avermitilis
branched chain
new
Prior art date
Application number
EC1994001123A
Other languages
English (en)
Inventor
Claudio Daniel Denoya
Kim Jonelle Stutzman-Engwall
Original Assignee
Pfizer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer filed Critical Pfizer
Publication of ECSP941123A publication Critical patent/ECSP941123A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/04—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with a disulfide as acceptor (1.2.4)
    • C12Y102/04004—3-Methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (1.2.4.4), i.e. branched-chain-alpha-ketoacid dehydrogenase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención se refiere a nuevas secuencias de ADN que codifican el complejo alfa-cetoácido deshidrogenasa de cadena ramificada de un organismo que pertenece al género Streptomyces y a nuevos polipéptidos producidos por la expresión de tales secuencias. También se refiere a nuevos procedimientos de potenciar la producción de avermectina natural, de producir un mutante bkd de Streptomyces avermitilis y de producir nuevas avermectinas por fermentación.
EC1994001123A 1993-12-16 1994-07-20 Genes que codifican el complejo cetoacido deshidrogenasa de cadena ramificada de streptomyces avermitilis ECSP941123A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US16860293A 1993-12-16 1993-12-16

Publications (1)

Publication Number Publication Date
ECSP941123A true ECSP941123A (es) 1994-12-15

Family

ID=22612168

Family Applications (1)

Application Number Title Priority Date Filing Date
EC1994001123A ECSP941123A (es) 1993-12-16 1994-07-20 Genes que codifican el complejo cetoacido deshidrogenasa de cadena ramificada de streptomyces avermitilis

Country Status (21)

Country Link
US (1) US5707839A (es)
EP (1) EP0734443A1 (es)
JP (1) JP2800921B2 (es)
KR (1) KR100209842B1 (es)
CN (2) CN1056189C (es)
AU (2) AU6805894A (es)
BR (1) BR9408337A (es)
CA (1) CA2177269A1 (es)
CO (1) CO4340655A1 (es)
CZ (1) CZ174496A3 (es)
EC (1) ECSP941123A (es)
EG (1) EG22869A (es)
FI (1) FI942725L (es)
HU (1) HUT76092A (es)
IL (3) IL109991A (es)
NO (2) NO962544L (es)
NZ (3) NZ329675A (es)
PE (1) PE11096A1 (es)
PL (1) PL178993B1 (es)
WO (1) WO1995016781A1 (es)
ZA (1) ZA944092B (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5466102A (en) * 1993-12-16 1995-11-14 Kennametal Inc. System for coupling machine tools
US6248579B1 (en) 1998-02-13 2001-06-19 Pfizer Inc Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins
CN1521180A (zh) * 1998-02-13 2004-08-18 �Ʒ� 介导除虫菌素b2：b1比例的除虫链霉菌基因
US6197591B1 (en) 1998-09-14 2001-03-06 Pfizer Inc. Streptomyces avermitilis regulatory genes for increased avermectin production
BRPI0013119B8 (pt) * 1999-08-12 2021-05-25 Pah Usa 15 Llc molécula polinucleotídica isolada, vetor recombinante purificado, célula hospedeira procariótica e célula de streptomyces avermitilis mutada, método para obtenção de uma nova cepa de s. avermitilis e processo para produção de avermectinas
DE10356092B4 (de) * 2003-12-01 2008-04-03 Siemens Audiologische Technik Gmbh Hörgerät mit drahtlosem Übertragungssystem
CN121574200B (zh) * 2026-01-28 2026-05-05 中国科学院烟台海岸带研究所 一种具有抗炎活性的六肽lr6及其制备方法和应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0262154A1 (en) * 1985-12-17 1988-04-06 Lubrizol Genetics Inc. Isolation of genes for biosynthesis of polyketide antibiotics
IN167980B (es) * 1987-01-23 1991-01-19 Pfizer
US5077278A (en) * 1987-01-23 1991-12-31 Pfizer Inc. Non-natural demethylavermectins compositions and method of use
DE3881147T2 (de) * 1987-10-23 1993-09-02 Pfizer Verfahren zur herstellung von aglykonen von avermectin und sie enthaltende kulturen.
ES2079436T3 (es) * 1989-03-31 1996-01-16 Merck & Co Inc Clonacion de genes de streptomyces avermitilis para biosintesis de avermectinas y procedimientos para su uso.
US5252474A (en) * 1989-03-31 1993-10-12 Merck & Co., Inc. Cloning genes from Streptomyces avermitilis for avermectin biosynthesis and the methods for their use

Also Published As

Publication number Publication date
NO962544D0 (no) 1996-06-14
FI942725A0 (fi) 1994-06-09
IL154161A0 (en) 2003-07-31
WO1995016781A1 (en) 1995-06-22
CA2177269A1 (en) 1995-06-22
PL314977A1 (en) 1996-09-30
NZ266526A (en) 1997-12-19
EG22869A (en) 2003-09-30
AU6805894A (en) 1995-07-03
PE11096A1 (es) 1996-04-12
KR100209842B1 (ko) 1999-07-15
AU9421998A (en) 1999-03-04
JPH09500283A (ja) 1997-01-14
US5707839A (en) 1998-01-13
IL109991A (en) 2005-08-31
EP0734443A1 (en) 1996-10-02
NO20070802L (no) 1996-06-14
FI942725A7 (fi) 1995-06-17
CN1137806A (zh) 1996-12-11
NZ329117A (en) 1998-04-27
HUT76092A (en) 1997-06-30
NZ329675A (en) 1998-10-28
CN1210150A (zh) 1999-03-10
HU9601644D0 (en) 1996-08-28
BR9408337A (pt) 1997-08-19
CO4340655A1 (es) 1996-07-30
CZ174496A3 (cs) 1999-05-12
IL154162A0 (en) 2003-07-31
JP2800921B2 (ja) 1998-09-21
ZA944092B (en) 1995-12-11
NO962544L (no) 1996-06-14
IL109991A0 (en) 1994-10-07
PL178993B1 (pl) 2000-07-31
CN1056189C (zh) 2000-09-06
FI942725L (fi) 1995-06-17

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