EP1109916A1 - Für eine amylosucrase kodierende nucleinsäuremoleküle - Google Patents

Für eine amylosucrase kodierende nucleinsäuremoleküle

Info

Publication number
EP1109916A1
EP1109916A1 EP98948899A EP98948899A EP1109916A1 EP 1109916 A1 EP1109916 A1 EP 1109916A1 EP 98948899 A EP98948899 A EP 98948899A EP 98948899 A EP98948899 A EP 98948899A EP 1109916 A1 EP1109916 A1 EP 1109916A1
Authority
EP
European Patent Office
Prior art keywords
nucleic acid
amylosucrase
glucans
protein
fructose
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP98948899A
Other languages
English (en)
French (fr)
Inventor
Martin Quanz
Nicholas Provart
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Bioscience GmbH
Original Assignee
Planttec Biotechnologie GmbH Forschung and Entwicklung
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Planttec Biotechnologie GmbH Forschung and Entwicklung filed Critical Planttec Biotechnologie GmbH Forschung and Entwicklung
Publication of EP1109916A1 publication Critical patent/EP1109916A1/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)

Definitions

  • the present invention relates to nucleic acid molecules encoding a protein having amylosucrase activity and to vectors containing such molecules. Furthermore, the invention relates to the production of ⁇ -1 ,4 glucans and fructose using the described nucleic acid molecules or the encoded proteins.
  • Linear ⁇ -1 ,4 glucans are polysaccharides consisting of glucose monomers, the latter being exclusively linked to each other by ⁇ -1 ,4 glycosidic bonds.
  • the most frequently occurring natural ⁇ -1,4 glucan is amylose, a component of plant starch. Recently, more and more importance has been attached to the commercial use of linear ⁇ -1 ,4 glucans. Due to its physico-chemical properties amylose can be used to produce films that are colorless, odorless and flavorless, non-toxic and biologically degradable.
  • there are various possibilities of application e.g., in the food industry, the textile industry, the glass fiber industry and in the production of paper.
  • ⁇ -1 ,4 glucans in particular linear ⁇ -1 ,4 glucans, are obtained in the form of amylose from starch.
  • Starch itself consists of two components. One component forms the amylose as an unbranched chain of ⁇ -1 ,4 linked glucose units. The other component forms the amylopectin, a highly branched polymer from glucose units in which in addition to the ⁇ -1 ,4 links the glucose chains can also be branched via ⁇ -1 ,6 links. Due to their different structure and the resulting physico-chemical properties, the two components are also used for different fields of application. In order to be able to directly utilize the properties of the individual components, it is necessary to obtain them in pure form.
  • Both components can be obtained from starch, the process, however, requiring several purification steps and involving considerable time and money. Therefore, there is a need to find possibilities of obtaining both components of the starch in a uniform manner. It is known that certain bacteria, in particular those of the genus Neisseria produce enzymes capable of synthesizing linear ⁇ -1 ,4 glucans from sucrose. In order to be able to use such enzymes for the efficient production of ⁇ -1 ,4 glucans, it is necessary to isolate and characterize the corresponding DNA sequences.
  • the technical problem underlying the present invention is therefore to provide nucleic acid molecules and processes that allow the production of ⁇ -1 ,4 glucans.
  • nucleic acid molecules encoding a protein comprising the amino acid sequence as depicted in SEQ ID NO: 2;
  • nucleic acid molecules the sequence of which differs from the sequence of a nucleic acid molecule as defined in (c) due to the degeneracy of the genetic code.
  • the present invention also relates to nucleic acid molecules the complementary strand of which hybridizes under stringent conditions to a nucleic acid molecule as defined above and which encode a polypeptide having the enzymatic activity of an amylosucrase.
  • hybridization means a hybridization under stringent conditions as described for example in Sambrook et al., Molecular Cloning, A Laboratory Manual, 2 nd Edition (1989) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY).
  • Stringent conditions mean that there is a sequence identity of at least 80% of the complete coding sequence, preferably an identity of at least 90%, more preferably of at least 95% and particularly preferred of at least 99%.
  • Nucleic acid molecules hybridizing to the molecules according to the invention may be isolated e.g. from genomic or from cDNA libraries produced from organism expressing an amylosucrase, for example, from microorganisms, in particular from bacteria of the genus Neisseria.
  • the molecules hybridizing to the nucleic acid molecules of the invention also comprise fragments, derivatives and allelic variants of the above-described nucleic acid molecules which encode a protein having the enzymatic activity of an amylosucrase.
  • fragments are defined as parts of the nucleic acid molecules, which are long enough in order to encode a protein still having the enzymatic activity.
  • derivatives means that the sequences of these molecules differ from the sequences of the above-mentioned nucleic acid molecules at one or more positions and that they exhibit a high degree of homology to these sequences.
  • homology means that functional and/or structural equivalence exists between the respective nucleic acid molecules or the proteins they encode.
  • the nucleic acid molecules which are homologous to the above-described molecules and represent derivatives of these molecules, are generally variations of these molecules, that constitute modifications which exert the same biological function. These variations may be naturally occurring variations, for example sequences derived from other organisms, or mutations, whereby these mutations may have occurred naturally or they may have been introduced by means of a specific mutagenesis. Moreover the variations may be synthetically produced sequences.
  • the allelic variants may be naturally occurring as well as synthetically produced variants or variants produced by recombinant DNA techniques.
  • the proteins encoded by the various variants of the nucleic acid molecules according to the invention exhibit certain common characteristics.
  • Enzyme activity, molecular weight, immunologic reactivity, conformation etc. may belong to these characteristics as well as physical properties such as the mobility in gel electrophoresis, chromatographic characteristics, sedimentation coefficients, solubility, spectroscopic properties, stability, pH-optimum, temperature-optimum etc.
  • This reaction is a transglucosylation.
  • the transglucosylation can take place in the presence or absence of acceptor molecules.
  • acceptor molecules can be polysaccharides, such as maltooligosaccharides, dextrin, glycogen etc.
  • acceptor molecules can be polysaccharides, such as maltooligosaccharides, dextrin, glycogen etc.
  • the resulting product is a polymeric linear ⁇ -1 ,4-glucan.
  • a glucan is obtained which comprises a terminal fructose molecule. All the products obtainable by transglycosylation with the help of an amylosucrase in the absence or presence of an acceptor molecule are referred to in the scope of the present invention as ⁇ -1 ,4 glucans.
  • G-F+n(G-F) ⁇ G n -G-F+ ⁇ F wherein G-F is sucrose.
  • G glucose
  • F fructose
  • G n -G-F is an ⁇ -1 ,4 glucan.
  • G-F sucrose
  • F fructose
  • G is glucose.
  • the products of the reaction catalyzed by an amylosucrase are the above described ⁇ -1 ,4 glucans and fructose. Cofactors are not required.
  • Amylosucrase activity so far has been found only in few bacteria species, among them particularly the species Neisseria (MacKenzie et al., Can. J. Microbiol. 24 (1978), 357-362) and the enzyme has been examined only for its enzymatic activity. According to Okada et al., the partially purified enzyme from Neisseria perflava upon addition of sucrose results in the synthesis of glycogen-like polysaccharides which are branched to a small extent (Okada et al., J. Biol.
  • Neisseria The enzyme that is expressed in a constitutive manner in Neisseria is extremely stable, binds very strongly to the polymerization products and is competitively inhibited by the product fructose (MacKenzie et al., Can. J. Microbiol. 23 (1977), 1303-1307).
  • the Neisseria species Neisse ⁇ a polysaccharea secretes the amylosucrase (Riou et al., loc. cit.) while in the other Neisseria species it remains in the cell. Enzymes having amylosucrase activity could only be detected in microorganisms. Plants are not known to have amylosucrases.
  • the detection of the enzymatic activity of the amylosucrase can be achieved by detecting the synthesized glucans, as is described in Example 3, below. Detection is usually carried out by using a iodine stain. It is possible to identify bacterial colonies expressing amylosucrase by, e.g., treatment with iodine vapor. Colonies synthesizing the ⁇ -1 ,4 glucans are stained blue.
  • the enzyme activity of the purified enzyme can be detected on, e.g., sucrose- containing agarose plates. If the protein is applied to such a plate and incubated for about 1 h or more at 37°C, it diffuses into the agarose and catalyzes the synthesis of glucans. The latter can be detected by treatment with iodine vapor. Furthermore, the protein can be detected in native polyacrylamide gels. After a native polyacrylamide gel electrophoresis, the gel is equilibrated in sodium citrate buffer (50 mM, pH 6.5) and incubated over night in a sucrose solution (5% in sodium citrate buffer). If the gel is subsequently stained with Lugol's solution, areas in which proteins having amylosucrase activity are localized are stained blue due to the synthesis of ⁇ -1 ,4 glucans.
  • the invention relates to nucleic acid molecules encoding an amylosucrase from a microorganism, particularly a gram negative microorganism, preferably from a bacterium of the species Neisseria and particularly preferred from
  • nucleic acid molecules according to the invention can be any kind if nucleic acid molecule, for example, RNA or DNA, in particular cDNA or genomic DNA. They can be synthetic, partly synthetic or isolated from natural sources.
  • the present invention relates to vectors, for example, plasmids, phages, cosmids, phagemids or artificial chromosomes, containing a nucleic acid molecule according to the invention.
  • the invention particularly relates to vectors in which the nucleic acid molecule of the invention is linked to sequences ensuring expression of the nucleic acid molecule in prokaryotic or eukaryotic host cells.
  • Expression in this regard means transcription, preferably transcription and translation.
  • Expression vectors have been extensively described in the art.
  • a selection marker gene and a replication origin allowing replication in the selected host they normally contain a promoter active in the host cell and a transcription termination signal.
  • Promoters allowing a particularly strong expression of the gene downstream thereof are, e.g., the T7 promoter (Studier et al., in Methods in Enzymology 185 (1990), 60-89), lacuv ⁇ , trp, trp-lacUV5 (DeBoer et al., in Rodriguez, R.L. and Chamberlin, M.J., (Eds.), Promoters, Structure and Function; Praeger, New York, 1982, pp. 462-481 ; DeBoer et al., Proc. Natl. Acad. Sci.
  • the expression cassette is constructed from promoter, DNA sequence to be expressed and a DNA sequence allowing transcriptional termination and polyadenylation of the transcript. Promoters and transcriptional termination signals from Saccharomyces have also been described and are available.
  • An expression vector can be introduced into yeast cells by transformation according to standard techniques (Methods in Yeast Genetics, A Laboratory Course Manual, Cold Spring Harbor Laboratory Press, 1990). Cells containing the vector are selected and propagated on appropriate selection media. Yeasts furthermore allow to integrate the expression cassette via homologous recombination into the genome of a cell using an appropriate vector, leading to a stable inheritance of the feature.
  • the present invention relates to host cells transformed with a nucleic acid molecule or with a vector according to the invention.
  • Suitable host cells are prokaryotic cells, such as microorganisms, e.g. bacteria, such as E. coli, Bacillus, Streptococcus etc., or eukaryotic cells, e.g. fungal cells, such as Saccharomyces cerevisiae; plant cells or animal cells, e.g. insect cells, CHO cells etc.
  • the present invention relates to a process for producing a protein having amylosucrase activity comprising culturing a host cell according to the invention under conditions allowing expression of the protein and recovering the protein from the cells and/or the culture medium.
  • the present invention relates to a process for producing ⁇ -1 ,4 glucans and/or fructose comprising
  • the above described process now allows to produce pure ⁇ -1 ,4 glucans in vitro.
  • the amylosucrase expressed by Neisse ⁇ a polysaccharea is an extracellular enzyme which synthesizes linear ⁇ -1 ,4 glucans outside of the cells on the basis of sucrose. Unlike in the most pathways of synthesis for polysaccharides that proceed within the cell, neither activated glucose derivatives nor cofactors are required.
  • the energy that is required for the formation of the ⁇ -1 ,4 glucosidic link between the condensed glucose residues is directly obtained from the hydrolysis of the link between the glucose and the fructose unit in the sucrose molecule.
  • amylosucrase-secreting host cells in a sucrose- containing medium, with the secreted amylosucrase leading to a synthesis of ⁇ -1 ,4 glucans from sucrose in the medium.
  • These glucans can be isolated from the culture medium.
  • the process according to the invention allows to produce in an inexpensive manner pure fructose syrup.
  • Conventional methods for the production of fructose either contemplate the enzymatic hydrolysis of sucrose using an invertase or the degradation of starch into glucose units, often by acidolysis, and subsequent enzymatic conversion of the glucose into fructose by glucose isomerase. Both methods result in mixtures of glucose and fructose. The two components have to be separated from each other by chromatographic processes which are time consuming and expensive.
  • the separation of the substrate, sucrose, from the two reaction products, fructose and ⁇ -1 ,4 glucans, or separation of the two reaction products can be achieved by, e.g., using membranes allowing the permeation of fructose but not of sucrose or glucans. If the fructose is continuously removed via such a membrane, the sucrose is converted more or less completely into fructose and linear glucans.
  • amylosucrase producing cells can preferably be immobilized on a carrier material located between two membranes, one of which allows the permeation of fructose but not of sucrose or glucans and the other allows the permeation of sucrose but not of glucans.
  • the substrate is supplied through the membrane which allows the permeation of sucrose.
  • the synthesized glucans remain in the space between the two membranes and the released fructose can continuously be removed from the reaction equilibrium through the membrane which allows only the permeation of fructose.
  • Such a set-up allows an efficient separation of the reaction products and thus inter alia the production of pure fructose.
  • amylosucrases for the production of pure fructose offers the advantage that the comparably inexpensive substrate sucrose can be used as starting material and furthermore that the fructose can be isolated from the reaction mixture in a simple manner without chromatographic processes.
  • yeast cells expressing an amylosucrase can be used as a microorganism in the process. Cultivation methods for yeasts have been sufficiently described (Methods in Yeast Genetics, A Laboratory Course Manual, Cold Spring Harbor Laboratory Press, 1990).
  • yeasts secreting amylosucrase for the synthesis of ⁇ -1 ,4 glucans in sucrose-containing media is not readily possible as yeasts secrete an invertase that hydrolyzes extracellular sucrose.
  • the yeasts import the resulting hexoses via a hexose transporter.
  • Gozalbo and Hohmann describe a yeast strain that carries a defective suc2 gene and that therefore cannot secrete invertase.
  • these yeast cells do not contain a transport system for importing sucrose into the cells.
  • ⁇ -1 ,4 glucans are synthesized by the amylosucrase if the culture medium contains sucrose.
  • the fructose being formed as reaction product may subsequently be imported by the yeasts.
  • the present invention relates to a process for the production of ⁇ -1 ,4 glucans and/or fructose in vitro comprising the step of bringing a protein according to the invention into contact with a sucrose-containing solution under conditions allowing the conversion of sucrose to ⁇ -1 ,4 glucans and fructose and recovering the produced ⁇ -1 ,4 glucans and/or fructose from the solution.
  • the reaction conditions can be better controlled and that the reaction products are substantially purer and can more easily be further purified.
  • the enzyme can be purified from the culture medium by conventional purification techniques such as precipitation, ion exchange chromatography, affinity chromatography, gel filtration, HPLC reverse phase chromatography, etc. It is furthermore possible to express a polypeptide by modification of the DNA sequence inserted into the expression vector leading to a polypeptide which can be isolated more easily from the culture medium due to certain properties. It is possible to express the enzyme as a fusion protein along with another polypeptide sequence whose specific binding properties allow isolation of the fusion protein via affinity chromatography.
  • fusion protein e.g., expression as fusion protein along with glutathion S transferase and subsequent purification via affinity chromatography on a glutathion column, making use of the affinity of the glutathion S transferase to glutathion (Smith and Johnson, Gene 67 (1988), 31-40).
  • Another known technique is the expression as fusion protein along with the maltose binding protein (MBP) and subsequent purification on an amylose column (Guan et al., Gene 67 (1988), 21-30; Maina et al., Gene 74 (1988), 365-373).
  • the amylosucrase in such a process is immobilized.
  • immobilization offers the advantage that the enzyme as synthesis catalyst can easily be retrieved and can be used several times. Since the purification of enzymes usually is very time and cost intensive, an immobilization and reuse of the enzyme contributes to a considerable reduction of the costs.
  • Another advantage is the high degree of purity of the reaction products which inter alia is due to the fact that the reaction conditions can be better controlled when immobilized enzymes are used. The insoluble linear glucans yielded as reaction products can then be easily purified further.
  • carrier materials available for the immobilization of proteins which can be coupled to the carrier material either by covalent or non-covalent links (for an overview see: Methods in Enzymology Vol. 135, 136 and 137).
  • Widely used carrier materials are, e.g., agarose, cellulose, polyacrylamide, silica or nylon.
  • Cyclodextrins are produced by the degradation of starch by the enzyme cyclodextrin transglycosyiase (EC 2.4.1.19) which is obtained from the bacterium Bacillus macerans. Due to the branching of starch only about 40% of the glucose units can be converted to cyclodextrins using this system.
  • the concentrated supernatant was added to 50 ml of a sucrose solution (5% sucrose in 50 mM sodium citrate buffer pH 6.5). The entire solution was incubated at 37°C. Whitish insoluble polysaccharides are formed.
  • PCR polymerase chain reaction
  • the resulting fragment contains the coding region for amylosucrase except for the nucleotides coding for the 16 N-terminal amino acids. These amino acids comprise the sequences that appear to be necessary for the secretion of the enzyme from the cell. Furthermore, this PCR fragment contains 88 bp of the 3' untranslated region. By way of the primers used ⁇ /coi restriction sites were introduced into both ends of the fragment.
  • the resulting fragment was ligated with the A/col digested expression vector pMex 7.
  • the ligation products were transformed in E. coli cells and transformed clones were selected. Positive clones were incubated over night at 37°C on YT plates (1.5% agar; 100 ⁇ g/ml ampicillin; 5% sucrose; 0.2 mM IPTG). After subjecting the plates to iodine vapor no blue staining could be observed in the area surrounding the bacteria colonies, but the intracellular production of glycogen could be detected (brown staining of transformed cells in contrast to no staining in nontransformed XL1-Blue cells).
  • transformed cells cultivated on YT medium were broken up by ultrasound and the obtained crude extract was pipetted onto sucrose- containing agar plates. After subjecting the plates to iodine vapor a blue stain could be observed.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
EP98948899A 1998-09-02 1998-09-02 Für eine amylosucrase kodierende nucleinsäuremoleküle Withdrawn EP1109916A1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP1998/005573 WO2000014249A1 (en) 1998-09-02 1998-09-02 Nucleic acid molecules encoding an amylosucrase

Publications (1)

Publication Number Publication Date
EP1109916A1 true EP1109916A1 (de) 2001-06-27

Family

ID=8167048

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98948899A Withdrawn EP1109916A1 (de) 1998-09-02 1998-09-02 Für eine amylosucrase kodierende nucleinsäuremoleküle

Country Status (6)

Country Link
EP (1) EP1109916A1 (de)
JP (1) JP2002524080A (de)
AU (1) AU9535798A (de)
CA (1) CA2342124A1 (de)
HU (1) HUP0103414A3 (de)
WO (1) WO2000014249A1 (de)

Families Citing this family (185)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1117822B1 (de) * 1998-10-09 2006-05-03 Südzucker AG Mannheim/Ochsenfurt Herstellung von polyglucanen durch amylosucrase in gegenwart einer transferase
US7189288B2 (en) 2004-10-08 2007-03-13 Tate & Lyle Ingredients Americas, Inc. Enzyme-resistant starch and method for its production
US7276126B2 (en) 2005-06-03 2007-10-02 Tate And Lyle Ingredients Americas, Inc. Production of enzyme-resistant starch by extrusion
US8993039B2 (en) 2006-01-25 2015-03-31 Tate & Lyle Ingredients Americas Llc Fiber-containing carbohydrate composition
US8057840B2 (en) 2006-01-25 2011-11-15 Tate & Lyle Ingredients Americas Llc Food products comprising a slowly digestible or digestion resistant carbohydrate composition
US7608436B2 (en) 2006-01-25 2009-10-27 Tate & Lyle Ingredients Americas, Inc. Process for producing saccharide oligomers
CL2007003743A1 (es) 2006-12-22 2008-07-11 Bayer Cropscience Ag Composicion que comprende fenamidona y un compuesto insecticida; y metodo para controlar de forma curativa o preventiva hongos fitopatogenos de cultivos e insectos.
CL2007003744A1 (es) 2006-12-22 2008-07-11 Bayer Cropscience Ag Composicion que comprende un derivado 2-piridilmetilbenzamida y un compuesto insecticida; y metodo para controlar de forma curativa o preventiva hongos fitopatogenos de cultivos e insectos.
US20080286410A1 (en) * 2007-03-06 2008-11-20 Richmond Patricia A Production of Resistant Starch Product
EP1969934A1 (de) 2007-03-12 2008-09-17 Bayer CropScience AG 4-Cycloalkyl-oder 4-arylsubstituierte Phenoxyphenylamidine und deren Verwendung als Fungizide
WO2008110281A2 (de) 2007-03-12 2008-09-18 Bayer Cropscience Ag 3,4-disubstituierte phenoxyphenylamidine und deren verwendung als fungizide
WO2008110279A1 (de) 2007-03-12 2008-09-18 Bayer Cropscience Ag Dihalogenphenoxyphenylamidine und deren verwendung als fungizide
EP1969929A1 (de) 2007-03-12 2008-09-17 Bayer CropScience AG Substituierte Phenylamidine und deren Verwendung als Fungizide
BRPI0808846A2 (pt) 2007-03-12 2019-09-24 Bayer Cropscience Ag fenoxifenilamidinas 3-substituídas e seu uso como fungicidas
BRPI0810654B1 (pt) 2007-04-19 2016-10-04 Bayer Cropscience Ag tiadiazoliloxifenilamidinas, seu uso e seu método de preparação, composição e método para combate de micro-organismos indesejados, semente resistente a micro-organismo indesejado, bem como método para proteger a dita semente contra micro-organismos
DE102007045956A1 (de) 2007-09-26 2009-04-09 Bayer Cropscience Ag Wirkstoffkombination mit insektiziden und akariziden Eigenschaften
DE102007045922A1 (de) 2007-09-26 2009-04-02 Bayer Cropscience Ag Wirkstoffkombinationen mit insektiziden und akariziden Eigenschaften
DE102007045919B4 (de) 2007-09-26 2018-07-05 Bayer Intellectual Property Gmbh Wirkstoffkombinationen mit insektiziden und akariziden Eigenschaften
DE102007045920B4 (de) 2007-09-26 2018-07-05 Bayer Intellectual Property Gmbh Synergistische Wirkstoffkombinationen
DE102007045953B4 (de) 2007-09-26 2018-07-05 Bayer Intellectual Property Gmbh Wirkstoffkombinationen mit insektiziden und akariziden Eigenschaften
EP2090168A1 (de) 2008-02-12 2009-08-19 Bayer CropScience AG Methode zur Verbesserung des Pflanzenwachstums
EP2072506A1 (de) 2007-12-21 2009-06-24 Bayer CropScience AG Thiazolyloxyphenylamidine oder Thiadiazolyloxyphenylamidine und deren Verwendung als Fungizide
EP2168434A1 (de) 2008-08-02 2010-03-31 Bayer CropScience AG Verwendung von Azolen zur Steigerung der Resistenz von Pflanzen oder Pflanzenteilen gegenüber abiotischem Stress
AU2009281457A1 (en) 2008-08-14 2010-02-18 Bayer Cropscience Ag Insecticidal 4-phenyl-1H-pyrazoles
DE102008041695A1 (de) 2008-08-29 2010-03-04 Bayer Cropscience Ag Methoden zur Verbesserung des Pflanzenwachstums
EP2201838A1 (de) 2008-12-05 2010-06-30 Bayer CropScience AG Wirkstoff-Nützlings-Kombinationen mit insektiziden und akariziden Eigenschaften
EP2198709A1 (de) 2008-12-19 2010-06-23 Bayer CropScience AG Verfahren zur Bekämpfung resistenter tierischer Schädlinge
EP2204094A1 (de) 2008-12-29 2010-07-07 Bayer CropScience AG Verfahren zur verbesserten Verwendung des Herstellungspotentials von transgenen Pflanzen
US9763451B2 (en) 2008-12-29 2017-09-19 Bayer Intellectual Property Gmbh Method for improved use of the production potential of genetically modified plants
EP2223602A1 (de) 2009-02-23 2010-09-01 Bayer CropScience AG Verfahren zur verbesserten Nutzung des Produktionspotentials genetisch modifizierter Pflanzen
EP2039770A2 (de) 2009-01-06 2009-03-25 Bayer CropScience AG Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen
EP2039771A2 (de) 2009-01-06 2009-03-25 Bayer CropScience AG Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen
EP2039772A2 (de) 2009-01-06 2009-03-25 Bayer CropScience AG Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen
WO2010081689A2 (en) 2009-01-19 2010-07-22 Bayer Cropscience Ag Cyclic diones and their use as insecticides, acaricides and/or fungicides
EP2227951A1 (de) 2009-01-23 2010-09-15 Bayer CropScience AG Verwendung von Enaminocarbonylverbindungen zur Bekämpfung von durch Insekten übertragenen Viren
ES2406131T3 (es) 2009-01-28 2013-06-05 Bayer Intellectual Property Gmbh Derivados fungicidas de N-cicloalquil-N-biciclometileno-carboxamina
AR075126A1 (es) 2009-01-29 2011-03-09 Bayer Cropscience Ag Metodo para el mejor uso del potencial de produccion de plantas transgenicas
EP2218717A1 (de) 2009-02-17 2010-08-18 Bayer CropScience AG Fungizide N-((HET)Arylethyl)Thiocarboxamid-Derivative
JP5728735B2 (ja) 2009-02-17 2015-06-03 バイエル・インテレクチュアル・プロパティ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツングＢａｙｅｒ Ｉｎｔｅｌｌｅｃｔｕａｌ Ｐｒｏｐｅｒｔｙ ＧｍｂＨ 殺菌性ｎ−（フェニルシクロアルキル）カルボキサミド、ｎ−（ベンジルシクロアルキル）カルボキサミド及びチオカルボキサミド誘導体
TW201031331A (en) 2009-02-19 2010-09-01 Bayer Cropscience Ag Pesticide composition comprising a tetrazolyloxime derivative and a fungicide or an insecticide active substance
EP2232995A1 (de) 2009-03-25 2010-09-29 Bayer CropScience AG Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen
US8828906B2 (en) 2009-03-25 2014-09-09 Bayer Cropscience Ag Active compound combinations having insecticidal and acaricidal properties
MA33140B1 (fr) 2009-03-25 2012-03-01 Bayer Cropscience Ag Combinaisons d'agents actifs ayant des proprietes insecticides et acaricides
CN102395271A (zh) 2009-03-25 2012-03-28 拜尔农作物科学股份公司 具有杀虫和杀螨特性的活性化合物结合物
CN102448305B (zh) 2009-03-25 2015-04-01 拜尔农作物科学股份公司 具有杀昆虫和杀螨虫特性的活性成分结合物
MX2011009732A (es) 2009-03-25 2011-09-29 Bayer Cropscience Ag Combinaciones de principios activos sinergicas.
EP2239331A1 (de) 2009-04-07 2010-10-13 Bayer CropScience AG Verfahren zur verbesserten Verwendung des Herstellungspotentials von transgenen Pflanzen
US8835657B2 (en) 2009-05-06 2014-09-16 Bayer Cropscience Ag Cyclopentanedione compounds and their use as insecticides, acaricides and/or fungicides
EP2251331A1 (de) 2009-05-15 2010-11-17 Bayer CropScience AG Fungizide Pyrazolcarboxamid-Derivate
AR076839A1 (es) 2009-05-15 2011-07-13 Bayer Cropscience Ag Derivados fungicidas de pirazol carboxamidas
EP2255626A1 (de) 2009-05-27 2010-12-01 Bayer CropScience AG Verwendung von Succinat Dehydrogenase Inhibitoren zur Steigerung der Resistenz von Pflanzen oder Pflanzenteilen gegenüber abiotischem Stress
CN102595889A (zh) 2009-06-02 2012-07-18 拜耳作物科学公司 琥珀酸脱氢酶抑制剂在控制核盘菌属真菌中的应用
KR20120051015A (ko) 2009-07-16 2012-05-21 바이엘 크롭사이언스 아게 페닐 트리아졸을 함유하는 상승적 활성 물질 배합물
WO2011015524A2 (en) 2009-08-03 2011-02-10 Bayer Cropscience Ag Fungicide heterocycles derivatives
EP2292094A1 (de) 2009-09-02 2011-03-09 Bayer CropScience AG Wirkstoffkombinationen
EP2343280A1 (de) 2009-12-10 2011-07-13 Bayer CropScience AG Fungizid-Chinolinderivate
EP2519103B1 (de) 2009-12-28 2014-08-13 Bayer Intellectual Property GmbH Fungizide Hydroximoyl-Tetrazol-Derivate
KR20120102142A (ko) 2009-12-28 2012-09-17 바이엘 크롭사이언스 아게 살진균제 히드록시모일-헤테로사이클 유도체
CN102725282B (zh) 2009-12-28 2015-12-16 拜尔农科股份公司 杀真菌剂肟基-四唑衍生物
EA022553B1 (ru) 2010-01-22 2016-01-29 Байер Интеллектуэль Проперти Гмбх Применение комбинации биологически активных веществ, набор и средство, содержащие комбинацию биологически активных веществ, для борьбы с вредителями животного происхождения и способ улучшения использования продукционного потенциала трансгенного растения
ES2523503T3 (es) 2010-03-04 2014-11-26 Bayer Intellectual Property Gmbh 2-Amidobencimidazoles sustituidos con fluoroalquilo y su uso para el aumento de la tolerancia al estrés en plantas
JP2013523795A (ja) 2010-04-06 2013-06-17 バイエル・インテレクチユアル・プロパテイー・ゲー・エム・ベー・ハー 植物のストレス耐性を増強させるための４−フェニル酪酸及び／又はその塩の使用
BR112012025848A2 (pt) 2010-04-09 2015-09-08 Bayer Ip Gmbh uso de derivados do ácido (1-cianociclopropil) fenilfosfínico, os ésteres do mesmo e/ou os sais do mesmo para aumentar a tolerância de plantas a estresse abiótico.
US20130116287A1 (en) 2010-04-28 2013-05-09 Christian Beier Fungicide hydroximoyl-heterocycles derivatives
BR112012027558A2 (pt) 2010-04-28 2015-09-15 Bayer Cropscience Ag ''composto da fórmula (i), composição fungicida e método para o controle de fungos fitogênicos de colheitas''
WO2011134911A2 (en) 2010-04-28 2011-11-03 Bayer Cropscience Ag Fungicide hydroximoyl-tetrazole derivatives
WO2011151370A1 (en) 2010-06-03 2011-12-08 Bayer Cropscience Ag N-[(het)arylalkyl)] pyrazole (thio)carboxamides and their heterosubstituted analogues
UA110703C2 (uk) 2010-06-03 2016-02-10 Байєр Кропсайнс Аг Фунгіцидні похідні n-[(тризаміщений силіл)метил]-карбоксаміду
AU2011260332B2 (en) 2010-06-03 2014-10-02 Bayer Cropscience Ag N-[(het)arylethyl)] pyrazole(thio)carboxamides and their heterosubstituted analogues
CN109504700A (zh) 2010-06-09 2019-03-22 拜尔作物科学公司 植物基因组改造中常用的在核苷酸序列上修饰植物基因组的方法和工具
US9593317B2 (en) 2010-06-09 2017-03-14 Bayer Cropscience Nv Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering
CN103003246B (zh) 2010-07-20 2015-11-25 拜尔农科股份公司 作为抗真菌剂的苯并环烯烃
WO2012028578A1 (de) 2010-09-03 2012-03-08 Bayer Cropscience Ag Substituierte anellierte pyrimidinone und dihydropyrimidinone
EP2460406A1 (de) 2010-12-01 2012-06-06 Bayer CropScience AG Verwendung von Fluopyram zum Steuern von Nematoden in nematodresistentem Pflanzen
BR112013006611B1 (pt) 2010-09-22 2021-01-19 Bayer Intellectual Property Gmbh método para o controle do nematoide de cisto de soja (heterodera glycines) infestando uma planta de soja resistente a nematoide compreendendo a aplicação de n- {2- [3-cloro-5- (trifluorometil)-2-piridinil]etil}-2-(trifluorometil) benzamida (fluorpiram
US9408391B2 (en) 2010-10-07 2016-08-09 Bayer Intellectual Property Gmbh Fungicide composition comprising a tetrazolyloxime derivative and a thiazolylpiperidine derivative
BR112013009580B1 (pt) 2010-10-21 2018-06-19 Bayer Intellectual Property Gmbh Composto de fómrula (i), composição fungicida e método para controlar fungos fitopatogênicos
EP2630135B1 (de) 2010-10-21 2020-03-04 Bayer Intellectual Property GmbH 1-(heterocyclische carbonyl)-piperidine
CA2815117A1 (en) 2010-11-02 2012-05-10 Bayer Intellectual Property Gmbh N-hetarylmethyl pyrazolylcarboxamides
US20130231303A1 (en) 2010-11-15 2013-09-05 Bayer Intellectual Property Gmbh 5-halogenopyrazole(thio)carboxamides
CN103391925B (zh) 2010-11-15 2017-06-06 拜耳知识产权有限责任公司 5‑卤代吡唑甲酰胺
AR083875A1 (es) 2010-11-15 2013-03-27 Bayer Cropscience Ag N-aril pirazol(tio)carboxamidas
KR20180096815A (ko) 2010-12-01 2018-08-29 바이엘 인텔렉쳐 프로퍼티 게엠베하 작물에서 선충류를 구제하고 수확량을 증가시키기 위한 플루오피람의 용도
EP2460407A1 (de) 2010-12-01 2012-06-06 Bayer CropScience AG Wirkstoffkombinationen umfassend Pyridylethylbenzamide und weitere Wirkstoffe
US20130289077A1 (en) 2010-12-29 2013-10-31 Juergen Benting Fungicide hydroximoyl-tetrazole derivatives
EP2474542A1 (de) 2010-12-29 2012-07-11 Bayer CropScience AG Fungizide Hydroximoyl-Tetrazol-Derivate
EP2471363A1 (de) 2010-12-30 2012-07-04 Bayer CropScience AG Verwendung von Aryl-, Heteroaryl- und Benzylsulfonamidocarbonsäuren, -carbonsäureestern, -carbonsäureamiden und -carbonitrilen oder deren Salze zur Steigerung der Stresstoleranz in Pflanzen
EP2494867A1 (de) 2011-03-01 2012-09-05 Bayer CropScience AG Halogen-substituierte Verbindungen in Kombination mit Fungiziden
BR112013022998A2 (pt) 2011-03-10 2018-07-03 Bayer Ip Gmbh método para aprimorar a germinação das sementes.
CN103502238A (zh) 2011-03-14 2014-01-08 拜耳知识产权有限责任公司 杀真菌剂肟基-四唑衍生物
CN103517900A (zh) 2011-04-08 2014-01-15 拜耳知识产权有限责任公司 杀真菌剂肟基-四唑衍生物
AR085568A1 (es) 2011-04-15 2013-10-09 Bayer Cropscience Ag 5-(biciclo[4.1.0]hept-3-en-2-il)-penta-2,4-dienos y 5-(biciclo[4.1.0]hept-3-en-2-il)-pent-2-en-4-inos sustituidos como principios activos contra el estres abiotico de las plantas
AR085585A1 (es) 2011-04-15 2013-10-09 Bayer Cropscience Ag Vinil- y alquinilciclohexanoles sustituidos como principios activos contra estres abiotico de plantas
EP2511255A1 (de) 2011-04-15 2012-10-17 Bayer CropScience AG Substituierte Prop-2-in-1-ol- und Prop-2-en-1-ol-Derivate
AR090010A1 (es) 2011-04-15 2014-10-15 Bayer Cropscience Ag 5-(ciclohex-2-en-1-il)-penta-2,4-dienos y 5-(ciclohex-2-en-1-il)-pent-2-en-4-inos sustituidos como principios activos contra el estres abiotico de las plantas, usos y metodos de tratamiento
WO2012143127A1 (en) 2011-04-22 2012-10-26 Bayer Cropsciences Ag Active compound combinations comprising a (thio)carboxamide derivative and a fungicidal compound
US20140173770A1 (en) 2011-06-06 2014-06-19 Bayer Cropscience Nv Methods and means to modify a plant genome at a preselected site
JP2014520776A (ja) 2011-07-04 2014-08-25 バイエル・インテレクチユアル・プロパテイー・ゲー・エム・ベー・ハー 植物における非生物的ストレスに対する活性薬剤としての置換されているイソキノリノン類、イソキノリンジオン類、イソキノリントリオン類およびジヒドロイソキノリノン類または各場合でのそれらの塩の使用
IN2014DN00156A (de) 2011-08-10 2015-05-22 Bayer Ip Gmbh
WO2013026836A1 (en) 2011-08-22 2013-02-28 Bayer Intellectual Property Gmbh Fungicide hydroximoyl-tetrazole derivatives
BR112014003919A2 (pt) 2011-08-22 2017-03-14 Bayer Cropscience Ag métodos e meios para modificar um genoma de planta
EP2561759A1 (de) 2011-08-26 2013-02-27 Bayer Cropscience AG Fluoralkyl-substituierte 2-amidobenzimidazole und ihre Wirkung auf das Pflanzenwachstum
RU2014113760A (ru) 2011-09-09 2015-10-20 Байер Интеллекчуал Проперти Гмбх Ацил-гомосериновые лактоновые производные для повышения урожая растений
CN103874681B (zh) 2011-09-12 2017-01-18 拜耳知识产权有限责任公司 杀真菌性4‑取代的‑3‑{苯基[(杂环基甲氧基)亚氨基]甲基}‑1,2,4‑噁二唑‑5(4h)‑酮衍生物
UA115971C2 (uk) 2011-09-16 2018-01-25 Байєр Інтеллектуал Проперті Гмбх Застосування ацилсульфонамідів для покращення врожайності рослин
EP2755484A1 (de) 2011-09-16 2014-07-23 Bayer Intellectual Property GmbH Verwendung von 5-phenyl- oder 5-benzyl-2-isoxazolin-3-carboxylaten für erhöhten pflanzenertrag
AR087873A1 (es) 2011-09-16 2014-04-23 Bayer Ip Gmbh Uso de fenilpirazolin-3-carboxilatos para mejorar el rendimiento de las plantas
BR112014006940A2 (pt) 2011-09-23 2017-04-04 Bayer Ip Gmbh uso de derivados de ácido 1-fenilpirazol-3-carboxílico 4-substituído como agentes contra estresse abiótico em plantas
ES2628436T3 (es) 2011-10-04 2017-08-02 Bayer Intellectual Property Gmbh ARNi para el control de hongos y oomicetos por la inhibición del gen de sacaropina deshidrogenasa
WO2013050324A1 (de) 2011-10-06 2013-04-11 Bayer Intellectual Property Gmbh Abiotischen pflanzenstress-reduzierende kombination enthaltend 4- phenylbuttersäure (4-pba) oder eines ihrer salze (komponente (a)) und eine oder mehrere ausgewählte weitere agronomisch wirksame verbindungen (komponente(n) (b)
WO2013075817A1 (en) 2011-11-21 2013-05-30 Bayer Intellectual Property Gmbh Fungicide n-[(trisubstitutedsilyl)methyl]-carboxamide derivatives
US9725414B2 (en) 2011-11-30 2017-08-08 Bayer Intellectual Property Gmbh Fungicidal N-bicycloalkyl and N-tricycloalkyl pyrazole-4-(thio)carboxamide derivatives
WO2013092519A1 (en) 2011-12-19 2013-06-27 Bayer Cropscience Ag Use of anthranilic acid diamide derivatives for pest control in transgenic crops
WO2013098147A1 (en) 2011-12-29 2013-07-04 Bayer Intellectual Property Gmbh Fungicidal 3-[(pyridin-2-ylmethoxyimino)(phenyl)methyl]-2-substituted-1,2,4-oxadiazol-5(2h)-one derivatives
CN104039769B (zh) 2011-12-29 2016-10-19 拜耳知识产权有限责任公司 杀真菌的3-[(1,3-噻唑-4-基甲氧基亚氨基)(苯基)甲基]-2-取代的-1,2,4-噁二唑-5(2h)-酮衍生物
US9408386B2 (en) 2012-02-22 2016-08-09 Bayer Intellectual Property Gmbh Use of succinate dehydrogenase inhibitors (SDHIs) for controlling wood diseases in grape
PE20190345A1 (es) 2012-02-27 2019-03-07 Bayer Ip Gmbh Combinaciones de compuestos activos
WO2013139949A1 (en) 2012-03-23 2013-09-26 Bayer Intellectual Property Gmbh Compositions comprising a strigolactame compound for enhanced plant growth and yield
JP2015517996A (ja) 2012-04-12 2015-06-25 バイエル・クロップサイエンス・アーゲーＢａｙｅｒ Ｃｒｏｐｓｃｉｅｎｃｅ Ａｇ 殺真菌剤として有用なｎ−アシル−２−（シクロ）アルキルピロリジンおよびピペリジン
EP2838363A1 (de) 2012-04-20 2015-02-25 Bayer Cropscience AG N-cycloalkyl-n-[(trisubstituiertes silylphenyl)methylen]-(thio)carboxamidderivate
AU2013251109B2 (en) 2012-04-20 2017-08-24 Bayer Cropscience Ag N-cycloalkyl-N-[(heterocyclylphenyl)methylene]-(thio)carboxamide derivatives
CN104245940A (zh) 2012-04-23 2014-12-24 拜尔作物科学公司 植物中的靶向基因组工程
EP2662360A1 (de) 2012-05-09 2013-11-13 Bayer CropScience AG 5-Halogenpyrazol-Indanyl-Carboxamide
EP2662370A1 (de) 2012-05-09 2013-11-13 Bayer CropScience AG 5-Halogenpyrazol-Benzofuranyl-Carboxamide
EP2662361A1 (de) 2012-05-09 2013-11-13 Bayer CropScience AG Pyrazol-Indanyl-Carboxamide
BR112014027644A2 (pt) 2012-05-09 2017-06-27 Bayer Cropscience Ag 5-halogenopirazol-indanil-carboxamidas
CN104768934B (zh) 2012-05-09 2017-11-28 拜耳农作物科学股份公司 吡唑茚满基甲酰胺
EP2662364A1 (de) 2012-05-09 2013-11-13 Bayer CropScience AG Pyrazol-Tetrahydronaphthyl-Carboxamide
EP2662363A1 (de) 2012-05-09 2013-11-13 Bayer CropScience AG 5-Halogenpyrazol-Biphenyl-Carboxamide
EP2662362A1 (de) 2012-05-09 2013-11-13 Bayer CropScience AG Pyrazol-Indanyl-Carboxamide
AR091104A1 (es) 2012-05-22 2015-01-14 Bayer Cropscience Ag Combinaciones de compuestos activos que comprenden un derivado lipo-quitooligosacarido y un compuesto nematicida, insecticida o fungicida
AU2013289301A1 (en) 2012-07-11 2015-01-22 Bayer Cropscience Ag Use of fungicidal combinations for increasing the tolerance of a plant towards abiotic stress
US20150216168A1 (en) 2012-09-05 2015-08-06 Bayer Cropscience Ag Use of substituted 2-amidobenzimidazoles, 2-amidobenzoxazoles and 2-amidobenzothiazoles or salts thereof as active substances against abiotic plant stress
CA2888559C (en) 2012-10-19 2021-03-02 Bayer Cropscience Ag Method for enhancing tolerance to abiotic stress in plants using carboxamide or thiocarboxamide derivatives
ES2665320T3 (es) 2012-10-19 2018-04-25 Bayer Cropscience Ag Procedimiento de tratamiento de plantas contra hongos resistentes a fungicidas usando derivados de carboxamida o de tiocarboxamida
CN105357968A (zh) 2012-10-19 2016-02-24 拜尔农科股份公司 包含羧酰胺衍生物的活性化合物复配物
PL2908640T3 (pl) 2012-10-19 2020-06-29 Bayer Cropscience Ag Sposób stymulowania wzrostu roślin przy pomocy pochodnych karboksamidu
WO2014079957A1 (de) 2012-11-23 2014-05-30 Bayer Cropscience Ag Selektive inhibition der ethylensignaltransduktion
EP2735231A1 (de) 2012-11-23 2014-05-28 Bayer CropScience AG Wirkstoffkombinationen
EP2925138A1 (de) 2012-11-30 2015-10-07 Bayer CropScience AG Ternäre fungizid- und pestizidmischungen
EP2925134B1 (de) 2012-11-30 2019-12-25 Bayer CropScience AG Ternäre fungizidmischungen
BR112015012473A2 (pt) 2012-11-30 2017-07-11 Bayer Cropscience Ag misturas binárias pesticidas e fungicidas
WO2014083033A1 (en) 2012-11-30 2014-06-05 Bayer Cropsience Ag Binary fungicidal or pesticidal mixture
WO2014083088A2 (en) 2012-11-30 2014-06-05 Bayer Cropscience Ag Binary fungicidal mixtures
EP2740720A1 (de) 2012-12-05 2014-06-11 Bayer CropScience AG Substituierte bicyclische- und tricyclische Pent-2-en-4-insäure -Derivate und ihre Verwendung zur Steigerung der Stresstoleranz in Pflanzen
WO2014086751A1 (de) 2012-12-05 2014-06-12 Bayer Cropscience Ag Verwendung substituierter 1-(arylethinyl)-, 1-(heteroarylethinyl)-, 1-(heterocyclylethinyl)- und 1-(cyloalkenylethinyl)-cyclohexanole als wirkstoffe gegen abiotischen pflanzenstress
EP2740356A1 (de) 2012-12-05 2014-06-11 Bayer CropScience AG Substituierte (2Z)-5(1-Hydroxycyclohexyl)pent-2-en-4-insäure-Derivate
AR093909A1 (es) 2012-12-12 2015-06-24 Bayer Cropscience Ag Uso de ingredientes activos para controlar nematodos en cultivos resistentes a nematodos
AR093996A1 (es) 2012-12-18 2015-07-01 Bayer Cropscience Ag Combinaciones bactericidas y fungicidas binarias
BR112015014307A2 (pt) 2012-12-19 2017-07-11 Bayer Cropscience Ag difluorometil-nicotínico- tetrahidronaftil carboxamidas
JP2016515100A (ja) 2013-03-07 2016-05-26 バイエル・クロップサイエンス・アクチェンゲゼルシャフト 殺菌性３−｛フェニル［（ヘテロシクリルメトキシ）イミノ］メチル｝−ヘテロ環誘導体
CA2908403A1 (en) 2013-04-02 2014-10-09 Bayer Cropscience Nv Targeted genome engineering in eukaryotes
EP2984080B1 (de) 2013-04-12 2017-08-30 Bayer CropScience Aktiengesellschaft Neuartige triazolderivate
CA2909213A1 (en) 2013-04-12 2014-10-16 Bayer Cropscience Aktiengesellschaft Novel triazole derivatives
BR112015026235A2 (pt) 2013-04-19 2017-10-10 Bayer Cropscience Ag método para melhorar a utilização do potencial de produção de plantas transgênicas envolvendo a aplicação de um derivado de ftaldiamida
US9554573B2 (en) 2013-04-19 2017-01-31 Bayer Cropscience Aktiengesellschaft Binary insecticidal or pesticidal mixture
WO2014177514A1 (en) 2013-04-30 2014-11-06 Bayer Cropscience Ag Nematicidal n-substituted phenethylcarboxamides
TW201507722A (zh) 2013-04-30 2015-03-01 Bayer Cropscience Ag 做為殺線蟲劑及殺體內寄生蟲劑的ｎ－（２－鹵素－２－苯乙基）－羧醯胺類
US9770022B2 (en) 2013-06-26 2017-09-26 Bayer Cropscience Ag N-cycloalkyl-N-[(bicyclylphenyl)methylene]-(thio)carboxamide derivatives
WO2015004040A1 (de) 2013-07-09 2015-01-15 Bayer Cropscience Ag Verwendung ausgewählter pyridoncarboxamide oder deren salzen als wirkstoffe gegen abiotischen pflanzenstress
US10071967B2 (en) 2013-12-05 2018-09-11 Bayer Cropscience Aktiengesellschaft N-cycloalkyl-N-{[2-(1-substitutedcycloalkyl)phenyl]methylene}-(thio)carboxamide derivatives
CN105793243A (zh) 2013-12-05 2016-07-20 拜耳作物科学股份公司 N-环烷基-n-{[2-(1-取代的环烷基)苯基]亚甲基}-(硫代)甲酰胺衍生物
AU2015223025B2 (en) * 2014-02-27 2018-09-20 Nutrition & Biosciences USA 4, Inc. Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes
AR101214A1 (es) 2014-07-22 2016-11-30 Bayer Cropscience Ag Ciano-cicloalquilpenta-2,4-dienos, ciano-cicloalquilpent-2-en-4-inas, ciano-heterociclilpenta-2,4-dienos y ciano-heterociclilpent-2-en-4-inas sustituidos como principios activos contra el estrés abiótico de plantas
AR103024A1 (es) 2014-12-18 2017-04-12 Bayer Cropscience Ag Piridoncarboxamidas seleccionadas o sus sales como sustancias activas contra estrés abiótico de las plantas
EP3283476B1 (de) 2015-04-13 2019-08-14 Bayer Cropscience AG N-cycloalkyl-n-[(biheterocyclylmethylen)-(thio)carboxamid-fungizide
WO2016205749A1 (en) 2015-06-18 2016-12-22 The Broad Institute Inc. Novel crispr enzymes and systems
BR112019001764A2 (pt) 2016-07-29 2019-05-07 Bayer Cropscience Ag combinações de compostos ativos e métodos para proteção de material de propagação de plantas
US20190281828A1 (en) 2016-09-22 2019-09-19 Bayer Cropscience Aktiengesellschaft Novel triazole derivatives
CN109715621A (zh) 2016-09-22 2019-05-03 拜耳作物科学股份公司 新的三唑衍生物
US20190225974A1 (en) 2016-09-23 2019-07-25 BASF Agricultural Solutions Seed US LLC Targeted genome optimization in plants
US12499971B2 (en) 2016-09-28 2025-12-16 The Broad Institute, Inc. Systematic screening and mapping of regulatory elements in non-coding genomic regions, methods, compositions, and applications thereof
WO2018077711A2 (en) 2016-10-26 2018-05-03 Bayer Cropscience Aktiengesellschaft Use of pyraziflumid for controlling sclerotinia spp in seed treatment applications
RU2755433C2 (ru) 2016-12-08 2021-09-16 Байер Кропсайенс Акциенгезельшафт Применение инсектицидов для борьбы с проволочниками
EP3332645A1 (de) 2016-12-12 2018-06-13 Bayer Cropscience AG Verwendung substituierter pyrimidindione oder jeweils deren salze als wirkstoffe gegen abiotischen pflanzenstress
WO2018108627A1 (de) 2016-12-12 2018-06-21 Bayer Cropscience Aktiengesellschaft Verwendung substituierter indolinylmethylsulfonamide oder deren salze zur steigerung der stresstoleranz in pflanzen
US11591601B2 (en) 2017-05-05 2023-02-28 The Broad Institute, Inc. Methods for identification and modification of lncRNA associated with target genotypes and phenotypes
WO2018213726A1 (en) 2017-05-18 2018-11-22 The Broad Institute, Inc. Systems, methods, and compositions for targeted nucleic acid editing
WO2019025153A1 (de) 2017-07-31 2019-02-07 Bayer Cropscience Aktiengesellschaft Verwendung von substituierten n-sulfonyl-n'-aryldiaminoalkanen und n-sulfonyl-n'-heteroaryldiaminoalkanen oder deren salzen zur steigerung der stresstoleranz in pflanzen
AU2018338318B2 (en) 2017-09-21 2022-12-22 Massachusetts Institute Of Technology Systems, methods, and compositions for targeted nucleic acid editing
WO2019126709A1 (en) 2017-12-22 2019-06-27 The Broad Institute, Inc. Cas12b systems, methods, and compositions for targeted dna base editing
US10968257B2 (en) 2018-04-03 2021-04-06 The Broad Institute, Inc. Target recognition motifs and uses thereof
EP3802521A1 (de) 2018-06-04 2021-04-14 Bayer Aktiengesellschaft Herbizid wirksame bizyklische benzoylpyrazole
CN113544266A (zh) 2018-12-17 2021-10-22 博德研究所 Crispr相关转座酶系统和其使用方法
US11540549B2 (en) 2019-11-28 2023-01-03 Tate & Lyle Solutions Usa Llc High-fiber, low-sugar soluble dietary fibers, products including them and methods for using them

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69535543T2 (de) * 1994-05-18 2008-04-30 Bayer Bioscience Gmbh Für enzyme, die die fähigkeit besitzen lineare alpha 1,4-glucane in pflanzen, pilzen und mikroorganismen zu synthesieren, kodierende dna sequenzen
DE4420223C1 (de) * 1994-06-06 1995-05-04 Inst Genbiologische Forschung Verfahren zur Kombination der intrazellulären Polyhydroxyalkanoat-Synthese in Mikroorganismen mit einer extrazellulären Polysaccharid-Synthese

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO0014249A1 *

Also Published As

Publication number Publication date
WO2000014249A1 (en) 2000-03-16
HUP0103414A3 (en) 2005-12-28
HUP0103414A2 (hu) 2002-01-28
AU9535798A (en) 2000-03-27
JP2002524080A (ja) 2002-08-06
CA2342124A1 (en) 2000-03-16

Similar Documents

Publication Publication Date Title
WO2000014249A1 (en) Nucleic acid molecules encoding an amylosucrase
CA2190149C (en) Dna sequences coding for enzymes capable of facilitating the synthesis of linear .alpha.-1,4 glucans in plants, fungi and microorganisms
AU695355B2 (en) Use of alpha-1,4-glucan lyase for preparation of 1,5-d-anhydrofructose
KR100387303B1 (ko) 비환원성 당질로부터 트레할로오스를 유리하는 재조합 열안정성 효소
DE69534685T2 (de) Transferase und amylase, verfahren zur herstellung dieser enzyme, ihre verwendung und für die kodierende gene
JP3958884B2 (ja) 非還元性糖質生成酵素及びトレハロース遊離酵素ならびに該酵素を用いる糖質の製造方法
KR100395445B1 (ko) 환원성 전분당으로부터 비환원성 당질을 생성하는 재조합 열안정성 효소
RU2272842C2 (ru) Линейные альфа-1,4-глюканы и способ их получения
US6632643B2 (en) Use of α-1,4-glucan lyase for preparation of 1,5-D-hydrofructose
JP4012931B2 (ja) 非還元性糖質生成酵素及びトレハロース遊離酵素ならびに該酵素を用いる糖質の製造方法
JP4012932B2 (ja) 非還元性糖質生成酵素及びトレハロース遊離酵素ならびに該酵素を用いる糖質の製造方法
CN111560077A (zh) 一种酶及其在合成普鲁兰多糖中的作用
JP2003250559A (ja) 糖転移反応を触媒する新規な酵素をコードする遺伝子および当該酵素の製造方法
DE4447388A1 (de) DNA-Sequenzen, die die Synthese linearer alpha-1,4-Glukane in Pflanzen, Pilzen und Mikroorganismen ermöglichen
JP2004305225A (ja) 非還元性糖質生成酵素及びトレハロース遊離酵素ならびに該酵素を用いる糖質の製造方法

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20010221

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: PLANTTEC BIOTECHNOLOGIE GMBH

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: BAYER BIOSCIENCE GMBH

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20060117