ES2010983A4 - Separacionm isoelectrica continua - Google Patents

Separacionm isoelectrica continua

Info

Publication number
ES2010983A4
ES2010983A4 ES89870001T ES89870001T ES2010983A4 ES 2010983 A4 ES2010983 A4 ES 2010983A4 ES 89870001 T ES89870001 T ES 89870001T ES 89870001 T ES89870001 T ES 89870001T ES 2010983 A4 ES2010983 A4 ES 2010983A4
Authority
ES
Spain
Prior art keywords
duct
protein
target protein
charged
isoelectric separation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
ES89870001T
Other languages
English (en)
Inventor
Donald Irvine Stimpson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monsanto Co
Original Assignee
Monsanto Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Co filed Critical Monsanto Co
Publication of ES2010983A4 publication Critical patent/ES2010983A4/es
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C07K1/28—Isoelectric focusing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)

Abstract

UN METODO Y APARATO PARA LA SEPARACION CONTINUA DE UNA PROTEINA DIANA DE UNA MEZCLA DE PROTEINA QUE CONTIENE DOS O MAS PROTEINAS Y A UN PH IGUAL AL PL DE LA PROTEINA DIANA SE DESCUBRE. EL METODO Y APARATO EMPLEADOS ES UN CONDUCTO POROSO CONDUCTOR NO ELECTRICAMENTE, NO IONICO (10) A TRAVES DEL CUAL SE PASA LA MEZCLA DE PROTEINA. EL CONDUCTO SE SOMETE A LA INFLUENCIA DE UN CAMPO ELECTRICO SUSTANCIALMENTE PERPENDICULAR A LA CORRIENTE O FLUJO DE ESTO RESULTANTE EN MOVIMIENTO DE TODA LA PROTEINA CARGADA DE LA LUZ DEL CONDUCTO. LA PROTEINA DIANA DE LA LUZ DEL CONDUCTO. LA PROTEINA DIANA QUE NO SE AFECTA EL CAMPO ELECTRICO YA QUE NO ESTA CARGADA AL MISMO PH QUE SU PL SE ALMACENA DESDE EL CONDUCTO SALE EN UNA FORNMA SUSTANCIALMENTE PURIFICADA.
ES89870001T 1988-01-05 1989-01-04 Separacionm isoelectrica continua Pending ES2010983A4 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14085588A 1988-01-05 1988-01-05
US07/273,780 US5114555A (en) 1988-01-05 1988-11-23 Continuous isoelectric separation

Publications (1)

Publication Number Publication Date
ES2010983A4 true ES2010983A4 (es) 1989-12-16

Family

ID=26838532

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89870001T Pending ES2010983A4 (es) 1988-01-05 1989-01-04 Separacionm isoelectrica continua

Country Status (6)

Country Link
US (1) US5114555A (es)
EP (1) EP0323948A3 (es)
JP (1) JPH026739A (es)
DK (1) DK1989A (es)
ES (1) ES2010983A4 (es)
NO (1) NO890038L (es)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2037273T3 (es) * 1987-04-11 1993-06-16 Ciba-Geigy Ag Proceso de focalizacion isoelectrica y medios para llevar a cabo dicho proceso.
GB2267502B (en) * 1992-05-28 1997-01-22 Aligena Ag Polymeric reaction products for use in immobilized buffered gels and membranes
WO1995014118A1 (en) * 1993-11-17 1995-05-26 Applied Hydrogel Technology Corporation Methods for the separation of biological materials
CN1051245C (zh) * 1995-01-20 2000-04-12 清华大学 制备型等电点电泳分离方法及设备
WO1998013689A1 (de) * 1996-09-28 1998-04-02 Fuhr Guenter Verfahren und vorrichtung zur isoelektrischen teilchentrennung
AUPP521298A0 (en) * 1998-08-12 1998-09-03 Life Therapeutics Limited Purification of fibrinogen
AUPP790698A0 (en) 1998-12-23 1999-01-28 Life Therapeutics Limited Separation of microorganisms
US20050224355A1 (en) * 1999-12-23 2005-10-13 Brendon Conlan Removal of biological contaminants
AUPP790898A0 (en) 1998-12-23 1999-01-28 Life Therapeutics Limited Renal dialysis
US7077942B1 (en) * 1999-12-23 2006-07-18 Gradipore Limited Removal of biological contaminants
AUPQ691400A0 (en) 2000-04-14 2000-05-11 Life Therapeutics Limited Separation of micromolecules
AUPQ697300A0 (en) 2000-04-18 2000-05-11 Life Therapeutics Limited Separation apparatus
WO2001078877A1 (en) 2000-04-18 2001-10-25 Gradipore Limited Electrophoresis separation and treatment of samples
GB0010957D0 (en) 2000-05-05 2000-06-28 Novartis Ag Compound & method
WO2001085830A1 (en) * 2000-05-05 2001-11-15 University Of Louisville Research Foundation, Inc. Improved electrophoresis gel support
US6923896B2 (en) * 2000-09-22 2005-08-02 The Texas A&M University System Electrophoresis apparatus and method
JP2004510170A (ja) * 2000-10-06 2004-04-02 グラディポア　リミテッド マルチポート型分離装置および方法
DE10063096C1 (de) * 2000-12-18 2002-09-12 Gerhard Weber Elektrophoresevorrichtung, Elektrophoreseverfahren unter Verwendung einer Elektrophoresevorrichtung und Verwendung der Elektrophoresevorrichtung
AUPR222300A0 (en) * 2000-12-21 2001-01-25 Life Therapeutics Limited Electrophoresis device and method
CN1549924A (zh) 2001-07-16 2004-11-24 �����ʸ���˹�ع�˾ 通过等电点分析生物分子的缓冲液的阵列
GB0121189D0 (en) * 2001-08-31 2001-10-24 Diagnoswiss Sa Apparatus and method for separating an analyte
DE212005000044U1 (de) * 2004-08-25 2007-04-05 Agilent Technologies Inc., Santa Clara Elektrophoretische Separation in einem bewegten Fluid
TWI341217B (en) * 2008-10-14 2011-05-01 Ind Tech Res Inst Membrane cleaning method and apparatus
US20220334033A1 (en) * 2021-04-20 2022-10-20 Brown University Electrical dissociation of tissue samples into single cells and/or smaller groups of cells

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2976576A (en) * 1956-04-24 1961-03-28 Wichterle Otto Process for producing shaped articles from three-dimensional hydrophilic high polymers
US3051640A (en) * 1959-09-21 1962-08-28 Armour Pharma Amino acid separation process
US3240692A (en) * 1961-03-13 1966-03-15 Swift & Co Electrophoretic fractionation of ampholytes
CH489259A (de) * 1968-06-08 1970-04-30 Dietzsch Gmbh Hans Joachim Verfahren zur Herstellung von kapillaren Austauschern
DE2861803D1 (en) * 1977-06-15 1982-07-01 Nat Res Dev Electrophoresis membranes, their use in a separation method and separation apparatus
JPS58147639A (ja) * 1982-02-26 1983-09-02 Hideyuki Nishizawa 連続電気泳動分離法および装置

Also Published As

Publication number Publication date
EP0323948A3 (en) 1991-11-21
DK1989D0 (da) 1989-01-04
NO890038D0 (no) 1989-01-04
JPH026739A (ja) 1990-01-10
DK1989A (da) 1989-07-06
NO890038L (no) 1989-07-06
EP0323948A2 (en) 1989-07-12
US5114555A (en) 1992-05-19

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