ES2047946T3 - Metodos de cultivos celulares para producir proteina c activada. - Google Patents

Metodos de cultivos celulares para producir proteina c activada.

Info

Publication number
ES2047946T3
ES2047946T3 ES90912701T ES90912701T ES2047946T3 ES 2047946 T3 ES2047946 T3 ES 2047946T3 ES 90912701 T ES90912701 T ES 90912701T ES 90912701 T ES90912701 T ES 90912701T ES 2047946 T3 ES2047946 T3 ES 2047946T3
Authority
ES
Spain
Prior art keywords
protein
cell culture
culture methods
producing activated
activated protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90912701T
Other languages
English (en)
Inventor
Anur Ashok Kumar
Donald C Foster
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zymogenetics Inc
Original Assignee
Zymogenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zymogenetics Inc filed Critical Zymogenetics Inc
Application granted granted Critical
Publication of ES2047946T3 publication Critical patent/ES2047946T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6464—Protein C (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

SE PRESENTAN METODOS PARA LA PRODUCCION DE UNA PROTEINA C ACTIVADA EN CELULAS DE MAMIFEROS TRANSFECTADOS DE MANERA ESTABLE. LAS CELULAS SE CULTIVAN EN UN MEDIO QUE NO CONTIENE MAS DE UN 0,1% DE SUERO Y LA PROTEINA C ACTIVADA SE SEPARA DE LAS CELULAS. TAMBIEN SE PRESENTA LA PROTEINA C ACTIVADA PRODUCIDA SEGUN ESTOS METODOS.
ES90912701T 1989-08-11 1990-08-07 Metodos de cultivos celulares para producir proteina c activada. Expired - Lifetime ES2047946T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US39286189A 1989-08-11 1989-08-11

Publications (1)

Publication Number Publication Date
ES2047946T3 true ES2047946T3 (es) 1994-03-01

Family

ID=23552314

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90912701T Expired - Lifetime ES2047946T3 (es) 1989-08-11 1990-08-07 Metodos de cultivos celulares para producir proteina c activada.

Country Status (10)

Country Link
EP (1) EP0485504B1 (es)
JP (1) JP3045307B2 (es)
AT (1) ATE97958T1 (es)
AU (1) AU6295390A (es)
CA (1) CA2064774C (es)
DE (1) DE69004964T2 (es)
DK (1) DK0485504T3 (es)
ES (1) ES2047946T3 (es)
IE (1) IE66340B1 (es)
WO (1) WO1991002065A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7168591A (en) * 1989-12-29 1991-07-24 Zymogenetics Inc. Hybrid protein c
US5618714A (en) * 1993-12-15 1997-04-08 Eli Lilly And Company Methods for producing protein C
DE19710190A1 (de) * 1997-03-12 1998-09-17 Immuno Ag Aktivierter Vitamin K-abhängiger Blutfaktor und Verfahren zu dessen Herstellung
GB9810999D0 (en) * 1998-05-21 1998-07-22 Goken Joop Materials and methods relating to the expression of genes

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3688900T3 (de) * 1985-06-27 1998-06-10 Univ Washington Expression von Protein C.
US4959318A (en) * 1985-06-27 1990-09-25 Zymogenetics, Inc. Expression of protein C
AU606582B2 (en) * 1985-09-06 1991-02-14 Codon Methods for the recovery of tissue plasminogen activator
DE3769872D1 (de) * 1986-07-11 1991-06-13 Miles Inc Herstellung von rekombinantem protein.
JPH0387173A (ja) * 1987-09-10 1991-04-11 Teijin Ltd ヒト活性化天然型ファクター８ｃの製造方法及びそれに用いる形質転換体
US4992373A (en) * 1987-12-04 1991-02-12 Eli Lilly And Company Vectors and compounds for direct expression of activated human protein C

Also Published As

Publication number Publication date
WO1991002065A1 (en) 1991-02-21
EP0485504A1 (en) 1992-05-20
CA2064774A1 (en) 1991-02-12
DE69004964D1 (de) 1994-01-13
JP3045307B2 (ja) 2000-05-29
ATE97958T1 (de) 1993-12-15
CA2064774C (en) 2000-01-11
DK0485504T3 (da) 1994-04-18
IE902912A1 (en) 1991-02-27
EP0485504B1 (en) 1993-12-01
JPH04507347A (ja) 1992-12-24
DE69004964T2 (de) 1994-03-24
AU6295390A (en) 1991-03-11
IE66340B1 (en) 1995-12-27

Similar Documents

Publication Publication Date Title
NO975674L (no) Fremgangsmåte for å regulere sialyleringen av proteiner fremstilt av pattedyrceller i kultur
EP1344819A3 (en) Human hematopoietic stem cell
FI873162A7 (fi) Menetelmä faktori VIII aktiviteetin omaavien proteiinien valmistamiseksi käyttäen mikrobi-isäntäsoluja; ekspressiovektorit, isäntäsolut, vasta-aineet
ES2072892T3 (es) Proteina receptora para un factor 2 estimulante de celulas b humanas.
EP0449950A4 (en) Cell culture medium for human liver epithelial cell line
ATE177472T1 (de) Expression von makrophagen-induzierbaren proteinen (mips) in hefezellen
ES8402346A1 (es) Procedimiento para la obtencion de lineas de celulas animales y humanas cultivables permanentemente.
YU86883A (en) Process for producing functional human urochinase proteins
FI885436A7 (fi) Fermenttori soluviljelmien viljelemiseksi
ES2055611T3 (es) Pdgf resistente a proteasas y metodos de uso.
ES2176224T3 (es) Proceso para la produccion de proteina.
ZA917561B (en) Media for culture of mammalian cells
AU6295390A (en) Cell culture methods for producing activated protein c
IL111983A0 (en) Methods for producing protein c
AU524979B2 (en) Purified serum factors in animal cell culture medium
DE69634363D1 (de) Vorläufer von normalen neuralen epithelzellen
AU631651B2 (en) Method for culturing cells
JPS6434371A (en) Periosteal bone forming material
DE69923595D1 (de) Herstellung von proteinen
AR243231A1 (es) Procedimiento para producir medios de cultivo.
IE871932L (en) Method for the preparation of proteins with factor viii¹activity by microbial host cells, expression vectors, host¹cells, antibodies
ZA875267B (en) Method for the preparation of proteins with factor viii activity by microbial host cells;expression vectors,host cells,antibodies

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 485504

Country of ref document: ES