ES2076939T3 - Lipasa recombinante de humicola y procedimiento para la produccion de lipasas recombinantes de humicola. - Google Patents

Lipasa recombinante de humicola y procedimiento para la produccion de lipasas recombinantes de humicola.

Info

Publication number
ES2076939T3
ES2076939T3 ES88307980T ES88307980T ES2076939T3 ES 2076939 T3 ES2076939 T3 ES 2076939T3 ES 88307980 T ES88307980 T ES 88307980T ES 88307980 T ES88307980 T ES 88307980T ES 2076939 T3 ES2076939 T3 ES 2076939T3
Authority
ES
Spain
Prior art keywords
recombinant
humicola
production
lipase
lipas
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88307980T
Other languages
English (en)
Inventor
Esper Boel
Ida Birgitte Huge-Jensen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DK450087A external-priority patent/DK450087D0/da
Priority claimed from DK656087A external-priority patent/DK656087D0/da
Priority claimed from DK205488A external-priority patent/DK205488D0/da
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Application granted granted Critical
Publication of ES2076939T3 publication Critical patent/ES2076939T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PROCESO PARA LA PRODUCCION DE UNA LIPOSA HUMICOLA RECOMBINANTE. EL PROCESO COMPRENDE EL CULTIVO DE ANFITRION ASPERGILLUS SP TRANSFORMADO CON UN SISTEMA VECTOR QUE COMPRENDE SECUENCIAS DE ADN QUE CODIFICAN FUNCIONES QUE FACILITAN LA EXPRESION DE GENES Y OPCIONALMENTE UN MARCADOR CONVENIETE PARA SELECCION DE TRANSFORMANTES, Y UNA SECUENCIA DE ADN QUE CODIFICA LA LIPOSA HUMICOLA. UN ORGANISMO ANFITRION PREFERIDO ES ASPERGILLUS ORIZAL. LA LIPOSA RECOMBINANTE HUMICOLA DE A.ORIZAL DIFIERE DE LA LIPOSA NATIVA EN QUE TIENE UNA MAYOR GLUCOSILACION Y MUESTRA MAYOR TERMOESTABILIDAD.
ES88307980T 1987-08-28 1988-08-26 Lipasa recombinante de humicola y procedimiento para la produccion de lipasas recombinantes de humicola. Expired - Lifetime ES2076939T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK450087A DK450087D0 (da) 1987-08-28 1987-08-28 Fremgangsmaade til fremstilling af proteiner
DK656087A DK656087D0 (da) 1987-12-15 1987-12-15 Enzym
DK205488A DK205488D0 (da) 1988-04-15 1988-04-15 Enzym

Publications (1)

Publication Number Publication Date
ES2076939T3 true ES2076939T3 (es) 1995-11-16

Family

ID=27221490

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88307980T Expired - Lifetime ES2076939T3 (es) 1987-08-28 1988-08-26 Lipasa recombinante de humicola y procedimiento para la produccion de lipasas recombinantes de humicola.

Country Status (5)

Country Link
EP (1) EP0305216B1 (es)
JP (1) JPH01157383A (es)
AT (1) ATE125865T1 (es)
DE (1) DE3854249T2 (es)
ES (1) ES2076939T3 (es)

Families Citing this family (846)

* Cited by examiner, † Cited by third party
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US5766912A (en) * 1986-03-17 1998-06-16 Novo Nordisk A/S Humicola lipase produced in aspergillus
US5830735A (en) * 1987-03-06 1998-11-03 Gist-Brocades Nv Method for producing lipolytic enzymes using transformed Pseudomonas
GB8810954D0 (en) * 1988-05-09 1988-06-15 Unilever Plc Enzymatic detergent & bleaching composition
US5292448A (en) * 1988-05-10 1994-03-08 Lever Brothers Company, Division Of Conopco, Inc. Enzymatic detergent composition
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GB8915658D0 (en) * 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
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JPH01157383A (ja) 1989-06-20
EP0305216B1 (en) 1995-08-02
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