ES2056821T3 - Enzimas quimericas. - Google Patents

Enzimas quimericas.

Info

Publication number
ES2056821T3
ES2056821T3 ES87305781T ES87305781T ES2056821T3 ES 2056821 T3 ES2056821 T3 ES 2056821T3 ES 87305781 T ES87305781 T ES 87305781T ES 87305781 T ES87305781 T ES 87305781T ES 2056821 T3 ES2056821 T3 ES 2056821T3
Authority
ES
Spain
Prior art keywords
amino acid
acid residues
terminal
alpha
amylase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES87305781T
Other languages
English (en)
Inventor
Boerge Krag Diderichsen
Helle Outtrup
Martin Schuelein
Barrie Edmund Norman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Application granted granted Critical
Publication of ES2056821T3 publication Critical patent/ES2056821T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

SE PRESENTAN ALFA-AMILASAS QUIMERICAS DE FORMULA Q-R-L EN LAS QUE Q ES UN RESTO POLIPEPTIDO N-TERMINAL DE 55 A 60 RESTOS AMINOACIDOS QUE ES AL MENOS EN UN 75% HOMOLOGO A LOS 55 RESTOS AMINOACIDOS N-TERMINALES DE LA ALFA-AMILASA DE BACILLUS AMYLOLIQUEFACIENS COMO SE DESCRIBE EN BIOL. CHEM., J.TAKKINER Y OTROS, 258 (1983) 1007-1013. R ES UN RESTO POLIPEPTIDO DE FORMULA GENERAL (I) EN LA QUE X8 COMPRENDE HIS O GIN; X9 COMPRENDE GLI O SER, Y X10 COMPRENDE SER O ASP, Y L COMPRENDE UN POLIPEPTIDO C.TERMINAL DE 390 A 400 RESTOS AMINOACIDOS QUE ES AL MENOS EN UN 75% HOMOLOGO A LA ALFA-AMILASA DE 395 RESTOS AMINOACIDOS C-TERMINALES DEL BACILLUS LICHENIFORMIS 584 (ATCC 27811).
ES87305781T 1986-06-30 1987-06-30 Enzimas quimericas. Expired - Lifetime ES2056821T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK311186A DK311186D0 (da) 1986-06-30 1986-06-30 Enzymer

Publications (1)

Publication Number Publication Date
ES2056821T3 true ES2056821T3 (es) 1994-10-16

Family

ID=8119209

Family Applications (1)

Application Number Title Priority Date Filing Date
ES87305781T Expired - Lifetime ES2056821T3 (es) 1986-06-30 1987-06-30 Enzimas quimericas.

Country Status (6)

Country Link
EP (1) EP0252666B1 (es)
JP (1) JP2582787B2 (es)
AT (1) ATE90965T1 (es)
DE (1) DE3786306T2 (es)
DK (1) DK311186D0 (es)
ES (1) ES2056821T3 (es)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD282028A5 (de) * 1989-02-16 1990-08-29 Akad Wissenschaften Ddr Verfahren zur herstellung einer thermostabilen, hybriden bacillus-beta-1,3-1,4-glukanase
JP2530942B2 (ja) * 1989-02-16 1996-09-04 カールスベルク エイ／エス 酵素に関する改良
WO1991000353A2 (en) * 1989-06-29 1991-01-10 Gist-Brocades N.V. MUTANT MICROBIAL α-AMYLASES WITH INCREASED THERMAL, ACID AND/OR ALKALINE STABILITY
DE69434635D1 (en) * 1993-10-08 2006-04-27 Novo Nordisk As Amylasevarianten
US5830837A (en) * 1994-11-22 1998-11-03 Novo Nordisk A/S Amylase variants
CA2173329C (en) * 1993-10-08 2011-07-12 Henrik Bisgard-Frantzen Amylase variants
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US20060257890A1 (en) * 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5837458A (en) * 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
AR000862A1 (es) 1995-02-03 1997-08-06 Novozymes As Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
ES2432519T3 (es) 1996-04-30 2013-12-04 Novozymes A/S Mutantes de alfa-amilasa
US6197070B1 (en) 1996-05-15 2001-03-06 The Procter & Gamble Company Detergent compositions comprising alpha combination of α-amylases for malodor stripping
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
FR2762616B1 (fr) * 1997-04-28 1999-07-16 Roquette Freres Procede de fabrication d'un hydrolysat d'amidon a haute teneur en dextrose
US6187576B1 (en) 1997-10-13 2001-02-13 Novo Nordisk A/S α-amylase mutants
US6204232B1 (en) 1997-10-30 2001-03-20 Novo Nordisk A/S α-amlase mutants
FR2770854B1 (fr) * 1997-11-07 2001-11-30 Rhone Poulenc Agrochimie Sequence adn d'un gene de l'hydroxy-phenyl pyruvate dioxygenase et obtention de plantes contenant un tel gene, tolerantes aux herbicides
US6537746B2 (en) 1997-12-08 2003-03-25 Maxygen, Inc. Method for creating polynucleotide and polypeptide sequences
KR20010041617A (ko) * 1998-03-09 2001-05-25 피아 스타르 전분으로부터 글루코스 시럽의 효소성 제조
BRPI9911086B1 (pt) 1998-06-10 2016-08-02 Novozymes As composição de limpeza, processo para tratar tecidos a máquina, e uso de uma mananase
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
WO2000060059A2 (en) 1999-03-30 2000-10-12 NovozymesA/S Alpha-amylase variants
JP5571274B2 (ja) 2000-03-08 2014-08-13 ノボザイムス アクティーゼルスカブ 改変された特性を有する変異体
US6768044B1 (en) 2000-05-10 2004-07-27 Bayer Cropscience Sa Chimeric hydroxyl-phenyl pyruvate dioxygenase, DNA sequence and method for obtaining plants containing such a gene, with herbicide tolerance
EP2308980A3 (en) 2000-08-01 2011-04-27 Novozymes A/S Alpha-amylase mutants with altered properties
US20020155574A1 (en) 2000-08-01 2002-10-24 Novozymes A/S Alpha-amylase mutants with altered properties
EP1975229A3 (en) 2000-10-13 2009-03-18 Novozymes A/S Alpha-amylase variant with altered properties
EP2292784A3 (en) 2001-02-21 2011-06-22 Verenium Corporation Enzymes having alpha amylase activity and methods of use thereof
US7659102B2 (en) 2001-02-21 2010-02-09 Verenium Corporation Amylases, nucleic acids encoding them and methods for making and using them
ATE449840T1 (de) 2001-05-15 2009-12-15 Novozymes As Alpha-amylasevariante mit veränderten eigenschaften
US7741092B2 (en) 2002-10-31 2010-06-22 Verenium Corporation Amylases, nucleic acids encoding them and methods for making and using them
ES2554635T3 (es) 2004-07-05 2015-12-22 Novozymes A/S Variantes de alfa-amilasa con propiedades alteradas
KR20100088675A (ko) 2007-11-05 2010-08-10 다니스코 유에스 인크. 변경된 특성을 지닌 바실러스 종 ｔｓ-23 알파-아밀라아제의 변이체
US8206966B2 (en) 2007-11-05 2012-06-26 Danisco Us Inc. Alpha-amylase variants with altered properties
AU2009212526A1 (en) 2008-02-04 2009-08-13 Danisco Us Inc. TS23 alpha-amylase variants with altered properties
MX2010013113A (es) 2008-06-06 2010-12-21 Danisco Inc Variantes de alfa-amilasa geobacillus stearothermophilus con propiedades mejoradas.
WO2010036515A1 (en) 2008-09-25 2010-04-01 Danisco Us Inc. Alpha-amylase blends and methods for using said blends
WO2011076897A1 (en) 2009-12-22 2011-06-30 Novozymes A/S Use of amylase variants at low temperature
US20110150955A1 (en) 2009-12-23 2011-06-23 Shannon Elizabeth Klingman Products and Methods for Reducing Malodor from the Pudendum
WO2011080353A1 (en) 2010-01-04 2011-07-07 Novozymes A/S Stabilization of alpha-amylases towards calcium depletion and acidic ph
EP3404087A1 (en) 2010-02-10 2018-11-21 Novozymes A/S Alpha-amylase variants with high stability in presence of a chelating agent
EP2357220A1 (en) 2010-02-10 2011-08-17 The Procter & Gamble Company Cleaning composition comprising amylase variants with high stability in the presence of a chelating agent
US9434932B2 (en) 2011-06-30 2016-09-06 Novozymes A/S Alpha-amylase variants
US12157869B2 (en) 2019-07-10 2024-12-03 Jeffrey Dean Lindsay Methods and compositions for reducing persistent odor in clothing and mitigating biofilms on various materials
WO2022081615A1 (en) * 2020-10-13 2022-04-21 Ginkgo Bioworks, Inc. Biosynthesis of cannabinoids and cannabinoid precursors

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3480411D1 (en) * 1983-07-06 1989-12-14 Gist Brocades Nv Molecular cloning and expression in industrial microorganism species
JPS61115496A (ja) * 1984-11-09 1986-06-03 Hitachi Ltd 殿粉の液化方法
EP0208491B1 (en) * 1985-07-03 1993-08-25 Genencor International, Inc. Hybrid polypeptides and process for their preparation

Also Published As

Publication number Publication date
DK311186D0 (da) 1986-06-30
EP0252666A2 (en) 1988-01-13
ATE90965T1 (de) 1993-07-15
DE3786306T2 (de) 1994-01-13
EP0252666B1 (en) 1993-06-23
DE3786306D1 (de) 1993-07-29
JPS6368084A (ja) 1988-03-26
EP0252666A3 (en) 1989-03-15
JP2582787B2 (ja) 1997-02-19

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