ES2075831T3 - Regulacion antisentido de expresion genica vegetal. - Google Patents

Regulacion antisentido de expresion genica vegetal.

Info

Publication number
ES2075831T3
ES2075831T3 ES87309853T ES87309853T ES2075831T3 ES 2075831 T3 ES2075831 T3 ES 2075831T3 ES 87309853 T ES87309853 T ES 87309853T ES 87309853 T ES87309853 T ES 87309853T ES 2075831 T3 ES2075831 T3 ES 2075831T3
Authority
ES
Spain
Prior art keywords
gene expression
plant gene
sense regulation
arn
base sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES87309853T
Other languages
English (en)
Inventor
Ian George Bridges
Wolfgang Walter Schuch
Donald Grierson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syngenta Ltd
Original Assignee
Zeneca Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=10607120&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2075831(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Zeneca Ltd filed Critical Zeneca Ltd
Application granted granted Critical
Publication of ES2075831T3 publication Critical patent/ES2075831T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8249Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/01Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01011Pectinesterase (3.1.1.11)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01015Polygalacturonase (3.2.1.15)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/11Antisense
    • C12N2310/111Antisense spanning the whole gene, or a large part of it

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Fruits And Vegetables (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

EL ADN RECOMBINANTE COMPRENDE SECUENCIAS BASE PROMOTORA Y TERMINADORA RESPECTIVAMENTE HACIA DELANTE Y HACIA DETRAS DE UNA SECUENCIA BASE INVERTIDA COMPLEMENTARIA A UNA SECUENCIA SUSTANCIAL DE BASES EN EL M-ARN DE POLIGALACTURONASA. EL M-ARN COMPLEMENTARIO PRODUCIDO ASI RETARDA EL REBLANDECIMIENTO DE FRUTOS, EN PARTICULAR DE TOMATES.
ES87309853T 1986-11-11 1987-11-06 Regulacion antisentido de expresion genica vegetal. Expired - Lifetime ES2075831T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB868626879A GB8626879D0 (en) 1986-11-11 1986-11-11 Dna

Publications (1)

Publication Number Publication Date
ES2075831T3 true ES2075831T3 (es) 1995-10-16

Family

ID=10607120

Family Applications (2)

Application Number Title Priority Date Filing Date
ES92117411T Expired - Lifetime ES2149162T3 (es) 1986-11-11 1987-11-06 Regulacion anti-sentido de la expresion de genes de plantas.
ES87309853T Expired - Lifetime ES2075831T3 (es) 1986-11-11 1987-11-06 Regulacion antisentido de expresion genica vegetal.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES92117411T Expired - Lifetime ES2149162T3 (es) 1986-11-11 1987-11-06 Regulacion anti-sentido de la expresion de genes de plantas.

Country Status (9)

Country Link
US (3) US5073676A (es)
EP (2) EP0532060B1 (es)
JP (1) JP3098238B2 (es)
AT (2) ATE194655T1 (es)
AU (4) AU643184B2 (es)
DE (2) DE3751466T2 (es)
ES (2) ES2149162T3 (es)
GB (1) GB8626879D0 (es)
GR (2) GR3017148T3 (es)

Families Citing this family (68)

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US5453566A (en) * 1986-03-28 1995-09-26 Calgene, Inc. Antisense regulation of gene expression in plant/cells
US5296376A (en) * 1986-11-04 1994-03-22 Imperial Chemical Industries Plc DNA, constructs, cells and plants derived therefrom
GB8927048D0 (en) * 1989-11-30 1990-01-31 Ici Plc Dna,constructs,cells and plants derived therefrom
GB8626879D0 (en) * 1986-11-11 1986-12-10 Ici Plc Dna
US6271033B1 (en) 1986-11-11 2001-08-07 Zeneca Limited Method for modifying production of fruit ripening enzyme
NZ224787A (en) * 1987-05-26 1990-08-28 Calgene Inc A dna construct for specifically modifying the phenotype of fruit as distinct from other plant tissue
GB8811115D0 (en) * 1988-05-11 1988-06-15 Ici Plc Tomatoes
US6054566A (en) 1988-02-26 2000-04-25 Biosource Technologies, Inc. Recombinant animal viral nucleic acids
US5316931A (en) * 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US6284492B1 (en) 1988-02-26 2001-09-04 Large Scale Biology Corporation Recombinant animal viral nucleic acids
NL8800756A (nl) * 1988-03-25 1989-10-16 Vereniging Voor Christelijk Wetenschappelijk Onderwijs Genetisch gemanipuleerde plantecellen en planten, alsmede daarvoor bruikbaar recombinant dna.
DE3908813A1 (de) * 1989-03-17 1990-09-20 Roehm Gmbh Verfahren zur expression eines aus aspergillus niger stammenden gens in einem aspergillus
GB8916213D0 (en) * 1989-07-14 1989-08-31 Ici Plc Dna constructs,cells and plants derived therefrom
EP0421919A3 (en) * 1989-09-02 1992-04-15 Ciba-Geigy Ag Polygalacturonase encoding fungal expression system
DE3929741A1 (de) * 1989-09-07 1991-03-28 Hoechst Ag Rna mit endoribonucleaseaktivitaet gegen mrna von reifungsgenen, ihre herstellung und ihre verwendung in pflanzen
GB8923716D0 (en) * 1989-10-20 1989-12-06 Ici Plc Dna,constructs,cells and plants derived therefrom
US5744364A (en) * 1989-10-20 1998-04-28 Zeneca Limited PTOM36 constructs and tomato cells transformed therewith
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US5168064A (en) * 1990-04-20 1992-12-01 The Regents Of The University Of California Endo-1,4-β-glucanase gene and its use in plants
US5585545A (en) * 1990-04-20 1996-12-17 The Regents Of The University Of California Endo-1,4-β-glucanase genes and their use in plants
GB9009307D0 (en) * 1990-04-25 1990-06-20 Ici Plc Dna,constructs,cells and plant derived therefrom
GB9021923D0 (en) * 1990-10-09 1990-11-21 Ici Plc Dna,dna constructs,cells and plants derived therefrom
GB9024323D0 (en) * 1990-11-08 1990-12-19 Ici Plc Regulation of gene expression
US5260205A (en) * 1990-11-14 1993-11-09 Philip Morris Incorporated Method of purifying putrescine N-methyltransferase from tobacco plant extract with a polyamine
JPH06505864A (ja) * 1990-12-21 1994-07-07 コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガナイゼイション トマト酵素エンドポリガラクツロナーゼPG1β−サブ単位の少なくとも1部をコードするDNA配列
BR9107191A (pt) * 1990-12-26 1994-06-14 Monsanto Co Controle de amadurecimento de frutos e senescência em plantas
GB9105420D0 (en) * 1991-03-14 1991-05-01 Ici Plc Expression of genes in transgenic plants
GB9127098D0 (en) * 1991-12-20 1992-02-19 Ici Plc Dna,dna constructs,cells and plants derived therefrom
ZA933177B (en) * 1992-05-08 1996-02-09 Univ Arizona Transgenic tomato plants with altered polygalacturonase isoforms
GB9210273D0 (en) * 1992-05-13 1992-07-01 Ici Plc Dna
US5367065A (en) * 1992-08-10 1994-11-22 The Trustees Of The University Of Pennsylvania Constitutive triple response gene and mutations
US5955652A (en) * 1992-06-16 1999-09-21 The Trustees Of The University Of Pennsylvania Plant genes for sensitivity to ethylene and pathogens
RU94046396A (ru) * 1992-06-29 1996-11-10 Джин Ширс Пти.Лтд. (AU) Нуклеиновая кислота, днк, вектор, способ получения растения или животного, способ получения клеток, способ создания животного, животное, трансгенное животное, трансгенное растение, плоды, черенки и семена, растительные клетки, способ вмешательства в репликацию вируса
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US4801540A (en) * 1986-10-17 1989-01-31 Calgene, Inc. PG gene and its use in plants
GB8626879D0 (en) * 1986-11-11 1986-12-10 Ici Plc Dna
NZ224787A (en) * 1987-05-26 1990-08-28 Calgene Inc A dna construct for specifically modifying the phenotype of fruit as distinct from other plant tissue

Also Published As

Publication number Publication date
AU5263293A (en) 1994-03-17
US5457281A (en) 1995-10-10
DE3751466T2 (de) 1996-01-18
GB8626879D0 (en) 1986-12-10
AU7435091A (en) 1991-07-11
ATE194655T1 (de) 2000-07-15
AU643184B2 (en) 1993-11-11
AU663811B2 (en) 1995-10-19
AU8095687A (en) 1988-05-12
US5073676A (en) 1991-12-17
EP0271988B1 (en) 1995-08-16
US5447867A (en) 1995-09-05
DE3752317D1 (de) 2000-08-17
EP0271988A2 (en) 1988-06-22
AU2831295A (en) 1996-01-04
JPS63164892A (ja) 1988-07-08
DE3751466D1 (de) 1995-09-21
AU633202B2 (en) 1993-01-21
GR3034607T3 (en) 2001-01-31
GR3017148T3 (en) 1995-11-30
EP0532060A1 (en) 1993-03-17
DE3752317T2 (de) 2000-11-30
JP3098238B2 (ja) 2000-10-16
ES2149162T3 (es) 2000-11-01
EP0532060B1 (en) 2000-07-12
EP0271988A3 (en) 1989-04-26
ATE126536T1 (de) 1995-09-15

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