ES2080772T3 - Deteccion del virus a de la influenza por reaccion en cadena de polimerasa (pcr) despues de una transcripcion inversa de una zona del gen viral de la hemoaglutinina. - Google Patents

Deteccion del virus a de la influenza por reaccion en cadena de polimerasa (pcr) despues de una transcripcion inversa de una zona del gen viral de la hemoaglutinina.

Info

Publication number
ES2080772T3
ES2080772T3 ES90116420T ES90116420T ES2080772T3 ES 2080772 T3 ES2080772 T3 ES 2080772T3 ES 90116420 T ES90116420 T ES 90116420T ES 90116420 T ES90116420 T ES 90116420T ES 2080772 T3 ES2080772 T3 ES 2080772T3
Authority
ES
Spain
Prior art keywords
pcr
reaction
detection
polymerase chain
reverse transcription
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90116420T
Other languages
English (en)
Inventor
Peter A Dr Prof Cerutti
Alberic Bressoud
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Behringwerke AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Behringwerke AG filed Critical Behringwerke AG
Application granted granted Critical
Publication of ES2080772T3 publication Critical patent/ES2080772T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

LA SENSIBILIDAD EN LA DETECCION DEL VIRUS INFLUENZA-A PUDO AUMENTARSE NOTABLEMENTE EFECTUANDO UNA REACCION EN CADENA DE POLIMERASA (PCR), UTILIZANDO SECUENCIAS DE OLIGONUCLEOTIDOS (AMPLIMEROS) PROCEDENTES DE REGIONES EN BASTANTE BUEN ESTADO DE CONSERVACION DEL GEN HEMAGGLUTININ DEL VIRUS, DESPUES DE UNA TRANSCRIPCION INVERSA (RT) PREVIA. LA TRANSCRIPCION INVERSA Y LA PCR SON INSENSIBLES FRENTE A MUTACIONES AISLADAS POR INTERCAMBIO BASICO, SIEMPRE QUE NO SE VEAN AFECTADOS LOS EXTREMOS 3'' DE LOS AMPLIMEROS.
ES90116420T 1989-09-02 1990-08-28 Deteccion del virus a de la influenza por reaccion en cadena de polimerasa (pcr) despues de una transcripcion inversa de una zona del gen viral de la hemoaglutinina. Expired - Lifetime ES2080772T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3929144A DE3929144A1 (de) 1989-09-02 1989-09-02 Nachweis von influenza-a-virus durch polymerase-kettenreaktion (pcr) nach reverser transkription eines bereichs des viralen haemagglutinin-gens

Publications (1)

Publication Number Publication Date
ES2080772T3 true ES2080772T3 (es) 1996-02-16

Family

ID=6388468

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90116420T Expired - Lifetime ES2080772T3 (es) 1989-09-02 1990-08-28 Deteccion del virus a de la influenza por reaccion en cadena de polimerasa (pcr) despues de una transcripcion inversa de una zona del gen viral de la hemoaglutinina.

Country Status (13)

Country Link
US (1) US5187060A (es)
EP (1) EP0416426B1 (es)
JP (1) JPH0398600A (es)
KR (1) KR100188383B1 (es)
AT (1) ATE130372T1 (es)
AU (1) AU624265B2 (es)
CA (1) CA2024442A1 (es)
DE (2) DE3929144A1 (es)
DK (1) DK0416426T3 (es)
ES (1) ES2080772T3 (es)
GR (1) GR3018519T3 (es)
IE (1) IE68762B1 (es)
PT (1) PT95165B (es)

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KR970005274B1 (ko) * 1990-08-14 1997-04-15 아이시스 파마슈티칼스, 인코포레이티드 안티센스 올리고뉴클레오티드에 의한 a형 인플루엔자 바이러스, 안 아아보스트레인(ann arbor strain) h2n2의 억제
DE69329376T2 (de) * 1992-12-30 2001-01-04 Samsung Electronics Co., Ltd. Verfahren zur Herstellung einer SOI-Transistor-DRAM
DE4433194C2 (de) * 1994-09-17 1996-08-29 Meier Ewert Herbert Univ Prof Technik zur molekularbiologischen Typisierung von Virusvarianten
US6015664A (en) * 1995-11-03 2000-01-18 Mcw Research Foundation Multiplex PCR assay using unequal primer concentrations to detect HPIV 1,2,3 and RSV A,B and influenza virus A, B
US5817457A (en) * 1996-02-07 1998-10-06 Ma Bioservices, Inc. Methods and kits for detecting viral reverse transcriptase activity in a sample using an acidic pH or an elevated temperature
US20030134293A1 (en) * 1999-11-16 2003-07-17 Zhiping Liu Method for rapid and accurate identification of microorganisms
US6368801B1 (en) 2000-04-12 2002-04-09 Molecular Staging, Inc. Detection and amplification of RNA using target-mediated ligation of DNA by RNA ligase
US6881835B2 (en) 2002-01-04 2005-04-19 Dr. Chip Biotechnology Inc. Detection of respiratory viruses
US9487823B2 (en) * 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US8043834B2 (en) * 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
CN1942577A (zh) * 2003-11-26 2007-04-04 比纳克斯公司 预测传染病状态的方法和试剂盒
EP1589119A1 (en) * 2004-04-19 2005-10-26 Academisch Medisch Centrum Detection of enterovirus RNA and method for reverse transcribing RNA
EP1747296A2 (en) * 2004-04-19 2007-01-31 Academisch Medisch Centrum Detection of viral nucleic acid and method for reverse transcribing rna
US8309303B2 (en) * 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
WO2006121773A2 (en) * 2005-05-06 2006-11-16 Gen-Probe Incorporated Compositions and assays to detect influenza virus a and b nucleic acids
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
EP1989326A4 (en) 2006-01-17 2009-09-30 Health Research Inc BIO-HETERODUPLEX TRACKING TEST
WO2008042450A2 (en) * 2006-01-20 2008-04-10 Lawrence Livermore National Security, Llc. Multiplex detection of respiratory pathogens
NZ571459A (en) 2006-03-29 2011-09-30 Merial Ltd Vaccine against streptococcus equi
EP1843158A1 (de) * 2006-04-05 2007-10-10 Micronas Holding GmbH Nachweissystem für Krankheitserreger
DE102006020885A1 (de) * 2006-05-05 2007-11-08 Qiagen Gmbh Einführung von Sequenzelementen in Nukleinsäuren
US9481912B2 (en) 2006-09-12 2016-11-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and identifying nucleic acid sequences in biological samples
US8652782B2 (en) 2006-09-12 2014-02-18 Longhorn Vaccines & Diagnostics, Llc Compositions and methods for detecting, identifying and quantitating mycobacterial-specific nucleic acids
US8097419B2 (en) 2006-09-12 2012-01-17 Longhorn Vaccines & Diagnostics Llc Compositions and method for rapid, real-time detection of influenza A virus (H1N1) swine 2009
US8080645B2 (en) 2007-10-01 2011-12-20 Longhorn Vaccines & Diagnostics Llc Biological specimen collection/transport compositions and methods
US9683256B2 (en) 2007-10-01 2017-06-20 Longhorn Vaccines And Diagnostics, Llc Biological specimen collection and transport system
US11041215B2 (en) 2007-08-24 2021-06-22 Longhorn Vaccines And Diagnostics, Llc PCR ready compositions and methods for detecting and identifying nucleic acid sequences
AU2008293504B2 (en) 2007-08-27 2012-04-12 Longhorn Vaccines & Diagnostics, Llc Immunogenic compositions and methods
US10004799B2 (en) 2007-08-27 2018-06-26 Longhorn Vaccines And Diagnostics, Llc Composite antigenic sequences and vaccines
US11041216B2 (en) 2007-10-01 2021-06-22 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and quantifying nucleic acid sequences in blood samples
CA2861667C (en) 2007-10-01 2017-06-13 Longhorn Vaccines And Diagnostics, Llc Biological specimen collection and transport system and methods of use
US8354230B2 (en) 2007-12-21 2013-01-15 Quest Diagnostics Investments Inc. Multiplex detection assay for influenza and RSV viruses
CN101363064B (zh) * 2008-09-28 2012-05-09 崔晓兰 一种用于检测甲型流感病毒载量的标准品及其检测方法
CN101403015B (zh) * 2008-10-29 2013-03-06 崔晓兰 一种用于检测白血病病毒载量的标准品及其检测方法
US9234249B2 (en) 2010-07-12 2016-01-12 Gen-Probe Incorporated Compositions and assays to detect swine H1N1 influenza A virus, seasonal H1 influenza A virus and seasonal H3 influenza A virus nucleic acids
EP2707496A1 (en) 2011-05-11 2014-03-19 Diagon Kft. Procedure for rapid determination of viruses using nucleic acid-based molecular diagnostics, and a kit for this purpose
WO2013112916A1 (en) 2012-01-26 2013-08-01 Longhorn Vaccines And Diagnostics, Llc Composite antigenic sequences and vaccines
WO2016183292A1 (en) 2015-05-14 2016-11-17 Longhorn Vaccines And Diagnostics, Llc Rapid methods for the extraction of nucleic acids from biological samples
CN113430301B (zh) * 2021-06-09 2024-01-02 上海伯杰医疗科技有限公司 一种呼吸道病原体检测用组合物、试剂盒及其应用

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US4357421A (en) * 1979-04-02 1982-11-02 G. D. Searle & Co. Synthetic gene coding for influenza hemagglutinin
US4583195A (en) * 1982-09-30 1986-04-15 Pitney Bowes Inc. Mailing system interface between a scale system processor and a serial data bus which interconnects peripheral subsystems

Also Published As

Publication number Publication date
US5187060A (en) 1993-02-16
DE59009876D1 (de) 1995-12-21
ATE130372T1 (de) 1995-12-15
KR910006496A (ko) 1991-04-29
GR3018519T3 (en) 1996-03-31
IE903176A1 (en) 1991-03-13
JPH0398600A (ja) 1991-04-24
DK0416426T3 (da) 1996-03-11
EP0416426A2 (de) 1991-03-13
KR100188383B1 (ko) 1999-06-01
EP0416426A3 (en) 1991-09-11
IE68762B1 (en) 1996-07-10
AU624265B2 (en) 1992-06-04
PT95165A (pt) 1991-05-22
PT95165B (pt) 1997-05-28
EP0416426B1 (de) 1995-11-15
DE3929144A1 (de) 1991-03-07
CA2024442A1 (en) 1991-03-03
AU6203290A (en) 1991-03-07

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