ES2174063T3 - Procedimiento para la amplificacion de secuencias especificas de acidonucleico. - Google Patents

Procedimiento para la amplificacion de secuencias especificas de acidonucleico.

Info

Publication number
ES2174063T3
ES2174063T3 ES96908918T ES96908918T ES2174063T3 ES 2174063 T3 ES2174063 T3 ES 2174063T3 ES 96908918 T ES96908918 T ES 96908918T ES 96908918 T ES96908918 T ES 96908918T ES 2174063 T3 ES2174063 T3 ES 2174063T3
Authority
ES
Spain
Prior art keywords
procedure
nucleic acid
polymorphism
amplification
acidonucleico
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96908918T
Other languages
English (en)
Inventor
Alison Velyian Todd
Caroline Jane Fuery
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Johnson and Johnson Research Pty Ltd
Original Assignee
Johnson and Johnson Research Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson and Johnson Research Pty Ltd filed Critical Johnson and Johnson Research Pty Ltd
Application granted granted Critical
Publication of ES2174063T3 publication Critical patent/ES2174063T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

LA PRESENTE INVENCION DESCRIBE UN PROCEDIMIENTO PARA DETECTAR UN POLIMORFISMO GENETICO EN UN INDIVIDUO. EN UNA FORMA, EL PROCEDIMIENTO INCLUYE LAS SIGUIENTES ETAPAS: (1) OBTENER UNA MUESTRA QUE CONTIENE ACIDO NUCLEICO DEL INDIVIDUO; (2) AMPLIFICAR LA MUESTRA DE ACIDO NUCLEICO DE LA ETAPA (1) MEDIANTE UN PROCEDIMIENTO QUE IMPLICA TERMOCICLACION Y CEBADORES, LLEVANDOSE A CABO LA AMPLIFICACION EN PRESENCIA DE UNA ENDONUCLEASA DE RESTRICCION TERMOESTABLE QUE MANTIENE SU ACTIVIDAD DURANTE LA TERMOCICLACION, HABIENDO SIDO SELECCIONADOS LOS CEBADORES DE FORMA QUE INTRODUCEN EN EL ACIDO NUCLEICO AMPLIFICADO A PARTIR DEL ACIDO NUCLEICO QUE INCLUYE EL POLIMORFISMO O A PARTIR DEL ACIDO NUCLEICO QUE INCLUYE EL POLIMORFISMO, UNA SECUENCIA RECONOCIDA POR LA ENDONUCLEASA DE RESTRICCION TERMOESTABLE; Y (3) ANALIZAR EL PRODUCTO DE LA ETAPA (2) PARA DETERMINAR LA PRESENCIA O LA AUSENCIA DEL POLIMORFISMO.
ES96908918T 1995-04-13 1996-04-12 Procedimiento para la amplificacion de secuencias especificas de acidonucleico. Expired - Lifetime ES2174063T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AUPN2452A AUPN245295A0 (en) 1995-04-13 1995-04-13 Assay for genetic abnormalities

Publications (1)

Publication Number Publication Date
ES2174063T3 true ES2174063T3 (es) 2002-11-01

Family

ID=3786805

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96908918T Expired - Lifetime ES2174063T3 (es) 1995-04-13 1996-04-12 Procedimiento para la amplificacion de secuencias especificas de acidonucleico.

Country Status (17)

Country Link
US (1) US6261768B1 (es)
EP (1) EP0871767B1 (es)
JP (1) JPH11503027A (es)
KR (1) KR19980703858A (es)
CN (1) CN1185181A (es)
AR (1) AR001619A1 (es)
AT (1) ATE214429T1 (es)
AU (1) AUPN245295A0 (es)
BR (1) BR9604814A (es)
CA (1) CA2218026A1 (es)
DE (1) DE69619864T2 (es)
DK (1) DK0871767T3 (es)
ES (1) ES2174063T3 (es)
IL (1) IL117884A0 (es)
TW (1) TW546382B (es)
WO (1) WO1996032500A1 (es)
ZA (1) ZA962941B (es)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1167811C (zh) * 1998-03-05 2004-09-22 约翰逊及约翰逊研究有限公司 酶原性核酸的检测方法,以及相关分子和试剂盒
EP2267127A1 (en) 1998-04-28 2010-12-29 Ortho-Clinical Diagnostics, Inc. Improved methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues
US6469159B1 (en) 1999-04-26 2002-10-22 Robert T. Belly Methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues
DK1206571T3 (da) 1999-05-04 2004-12-06 Ortho Clinical Diagnostics Inc Hurtig og effektiv indfangning af DNA fra pröve uden anvendelse af cellelyseringsreagens
AUPQ495700A0 (en) * 2000-01-05 2000-02-03 Johnson & Johnson Research Pty. Limited Method for concurrent amplification and real time detection of polymorphic nucleic acid sequences
US7262006B1 (en) 2000-05-01 2007-08-28 Ortho-Clinical Diagnostics, Inc. Rapid and efficient capture of DNA from sample without using cell lysing reagent
JP2004518412A (ja) * 2000-10-02 2004-06-24 オルソ−クリニカル　ダイアグノスティクス，インコーポレイティド Ｒａｓ突然変異の検出
US7052837B2 (en) * 2001-03-13 2006-05-30 The Board Of Trustees Of The University Of Arkansas Histoplasma capsulatum catalase sequences and their use in the detection of Histoplamsa capsulatum and histoplasmosis
US20030162174A1 (en) * 2001-06-11 2003-08-28 Sutherland John W. Detecting nucleic acid deletion sequences
KR20040009054A (ko) * 2002-07-22 2004-01-31 김철욱 인적 유전인자의 개체별 특성파악을 위한 유전자 검사방법
US20040054160A1 (en) * 2002-09-16 2004-03-18 Santona Pal Nucleic-acid ink compositions for arraying onto a solid support
DE10315640A1 (de) * 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
EP1627079A2 (en) * 2003-05-22 2006-02-22 Dow Agrosciences LLC High-throughput methods of screening dna for deletions and other mutations
CN101027412B (zh) * 2004-07-23 2017-04-26 环太平洋生物技术有限公司 用于膀胱癌检测的尿标记物
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
AU2008230813B2 (en) * 2007-03-26 2014-01-30 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
US8999634B2 (en) 2007-04-27 2015-04-07 Quest Diagnostics Investments Incorporated Nucleic acid detection combining amplification with fragmentation
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US9404150B2 (en) 2007-08-29 2016-08-02 Sequenom, Inc. Methods and compositions for universal size-specific PCR
CN101280338B (zh) * 2007-12-13 2011-08-17 中国人民解放军第三军医大学第一附属医院 一种检测核酸多态性的核酸扩增方法
US8206926B2 (en) 2008-03-26 2012-06-26 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
US20100279295A1 (en) * 2009-03-18 2010-11-04 Sequenom, Inc. Use of thermostable endonucleases for generating reporter molecules
US9487824B2 (en) * 2011-06-28 2016-11-08 Igor Kutyavin Methods and compositions for enrichment of nucleic acids in mixtures of highly homologous sequences
US9352312B2 (en) 2011-09-23 2016-05-31 Alere Switzerland Gmbh System and apparatus for reactions
KR102126744B1 (ko) 2013-03-14 2020-06-26 다카라 바이오 가부시키가이샤 내열성 미스매치 엔도뉴클레아제의 이용방법
WO2016039377A1 (ja) 2014-09-11 2016-03-17 タカラバイオ株式会社 耐熱性のミスマッチエンドヌクレアーゼの利用方法
JP6914831B2 (ja) * 2015-03-20 2021-08-04 タカラバイオ株式会社 標的核酸の高感度検出方法
CN114014472B (zh) * 2022-01-07 2022-04-26 山东玲珑轮胎股份有限公司 一种废旧轮胎回收中产生的废水处理方法及系统

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1284931C (en) * 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
DE69121791T2 (de) 1990-06-08 1997-01-23 Behringwerke Ag Verfahren zur quantitativen Bestimmung von DNS-Sequenzen
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
AU652214B2 (en) * 1991-01-31 1994-08-18 Becton Dickinson & Company Strand displacement amplification "Sealing Clerk - sealing is to be refunded"
US5547861A (en) 1994-04-18 1996-08-20 Becton, Dickinson And Company Detection of nucleic acid amplification
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5512441A (en) 1994-11-15 1996-04-30 American Health Foundation Quantative method for early detection of mutant alleles and diagnostic kits for carrying out the method

Also Published As

Publication number Publication date
US6261768B1 (en) 2001-07-17
CN1185181A (zh) 1998-06-17
MX9707885A (es) 1998-06-28
AUPN245295A0 (en) 1995-05-11
WO1996032500A1 (en) 1996-10-17
EP0871767A1 (en) 1998-10-21
EP0871767B1 (en) 2002-03-13
AR001619A1 (es) 1997-11-26
TW546382B (en) 2003-08-11
DE69619864D1 (de) 2002-04-18
JPH11503027A (ja) 1999-03-23
BR9604814A (pt) 2000-05-09
ZA962941B (en) 1996-12-04
EP0871767A4 (en) 1999-09-01
KR19980703858A (ko) 1998-12-05
IL117884A0 (en) 1996-08-04
ATE214429T1 (de) 2002-03-15
CA2218026A1 (en) 1996-10-17
DE69619864T2 (de) 2002-11-07
DK0871767T3 (da) 2002-07-08

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