ES2089232T3 - 5-enolpiruvilsiquimato-3-fosfato sintasas tolerantes del glifosato. - Google Patents

5-enolpiruvilsiquimato-3-fosfato sintasas tolerantes del glifosato.

Info

Publication number
ES2089232T3
ES2089232T3 ES91917090T ES91917090T ES2089232T3 ES 2089232 T3 ES2089232 T3 ES 2089232T3 ES 91917090 T ES91917090 T ES 91917090T ES 91917090 T ES91917090 T ES 91917090T ES 2089232 T3 ES2089232 T3 ES 2089232T3
Authority
ES
Spain
Prior art keywords
class
epsps
genes
glyphosate
tolerant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91917090T
Other languages
English (en)
Other versions
ES2089232T5 (es
Inventor
Gerard Francis Barry
Ganesh Murthy Kishore
Stephen Rogers Padgette
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monsanto Co
Original Assignee
Monsanto Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24304844&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2089232(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Monsanto Co filed Critical Monsanto Co
Application granted granted Critical
Publication of ES2089232T3 publication Critical patent/ES2089232T3/es
Publication of ES2089232T5 publication Critical patent/ES2089232T5/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • H—ELECTRICITY
    • H03—ELECTRONIC CIRCUITRY
    • H03H—IMPEDANCE NETWORKS, e.g. RESONANT CIRCUITS; RESONATORS
    • H03H3/00—Apparatus or processes specially adapted for the manufacture of impedance networks, resonating circuits, resonators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Manufacturing & Machinery (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Waveguide Connection Structure (AREA)

Abstract

SE PRESENTAN ENZIMAS DE LA CLASE II EPSPS QUE CODIFICAN GENES. LOS GENES SON UTILES EN LA PRODUCCION DE BACTERIAS Y PLANTAS TRANSFORMADAS QUE SON TOLERANTES A LOS HERBICIDAS DE GLICOSATO. LOS GENES DE LA CLASE II EPSPS COMPARTEN UNA PEQUEÑA HOMOLOGIA CON LOS GENES YA CONOCIDOS DE LA CLASE I EPSPS, Y NO SE HIBRIDIZAN CON SONDAS DE LA CLASE I EPSPS. LAS ENZIMAS DE LA CLASE II EPSPS SE CARACTERIZAN POR SER MAS CINETICAMENTE EFICIENTES QUE LA CLASE I EPSPS EN LA PRESENCIA DE GLIFOSATO. TAMBIEN SE PRESENTAN PLANTAS TRANSFORMADAS POR LA CLASE II EPSPS ASI COMO UN METODO PARA CONTROLAR DE FORMA SELECTIVA LAS MALAS HIERBAS EN COSECHAS AGRICOLAS.
ES91917090T 1990-08-31 1991-08-28 5-enolpiruvilsiquimato-3-fosfato sintasas tolerantes del glifosato. Expired - Lifetime ES2089232T5 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US576537 1984-02-03
US57653790A 1990-08-31 1990-08-31

Publications (2)

Publication Number Publication Date
ES2089232T3 true ES2089232T3 (es) 1996-10-01
ES2089232T5 ES2089232T5 (es) 2007-03-16

Family

ID=24304844

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91917090T Expired - Lifetime ES2089232T5 (es) 1990-08-31 1991-08-28 5-enolpiruvilsiquimato-3-fosfato sintasas tolerantes del glifosato.

Country Status (14)

Country Link
EP (1) EP0546090B2 (es)
JP (2) JP3173785B2 (es)
KR (1) KR920005467A (es)
AT (1) ATE139566T1 (es)
AU (1) AU655945B2 (es)
CA (1) CA2088661C (es)
DE (1) DE69120419T3 (es)
DK (1) DK0546090T4 (es)
ES (1) ES2089232T5 (es)
GR (1) GR3021058T3 (es)
RU (1) RU2146290C1 (es)
TW (1) TW232078B (es)
UA (1) UA32548C2 (es)
WO (1) WO1992004449A1 (es)

Families Citing this family (71)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7705215B1 (en) * 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5633435A (en) 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
GB9515941D0 (en) 1995-08-03 1995-10-04 Zeneca Ltd DNA constructs
US5928995A (en) * 1995-05-05 1999-07-27 E. I. Du Pont De Nemours And Company Herbicidal mixtures
CA2219121A1 (en) 1995-05-26 1996-11-28 Zeneca Limited A gene switch comprising an ecdysone receptor
AP927A (en) * 1996-03-29 2001-01-12 Monsanto Europe Sa New use of N-(phosphonomethyl) glycine and derivatives thereof.
US5773696A (en) 1996-03-29 1998-06-30 Monsanto Company Antifungal polypeptide and methods for controlling plant pathogenic fungi
US6610828B1 (en) 1996-05-24 2003-08-26 Syngenta Limited Heliothis ecdysone receptor
FR2751347B1 (fr) * 1996-07-16 2001-12-07 Rhone Poulenc Agrochimie Gene chimere a plusieurs genes de tolerance herbicide, cellule vegetale et plante tolerantes a plusieurs herbicides
WO1998020144A2 (en) * 1996-11-07 1998-05-14 Zeneca Limited Herbicide resistant plants
US6121436A (en) 1996-12-13 2000-09-19 Monsanto Company Antifungal polypeptide and methods for controlling plant pathogenic fungi
TR199902348T2 (xx) 1997-03-25 2000-01-21 Basf Aktiengesellschaft Herbisit tolerans� geli�tirmek �zere bitkilerde herbisit ba�lay�c� polipeptitlerin ekspresyonu
US6040497A (en) * 1997-04-03 2000-03-21 Dekalb Genetics Corporation Glyphosate resistant maize lines
DE69837916T2 (de) 1997-04-03 2008-02-28 DeKalb Genetics Corp., DeKalb Verwendung von glyphosat-resistente maislinien
DK1019516T3 (da) * 1997-10-07 2004-09-27 Embrapa Pesquisa Agropecuaria Fremgangsmåde til opnåelse af transgene bælgplanter (Leguminosae) indeholdende exogent DNA
FR2772787B1 (fr) 1997-12-24 2001-12-07 Rhone Poulenc Agrochimie Promoteur h3c4 de mais associe au premier intron de l'actine de riz, gene chimere le comprenant et plante transformee
US6303848B1 (en) * 1998-01-16 2001-10-16 Large Scale Biology Corporation Method for conferring herbicide, pest, or disease resistance in plant hosts
BR9908631A (pt) * 1998-03-09 2000-11-14 Monsanto Co Glifosato como gameticida
US5914451A (en) * 1998-04-06 1999-06-22 Monsanto Company Efficiency soybean transformation protocol
US6906245B1 (en) 1998-04-30 2005-06-14 Sumitomo Chemical Company, Limited Method for producing transgenic plants resistant to weed control compounds which disrupt the porphyrin pathways of plants
US6066786A (en) * 1998-06-17 2000-05-23 Pure Seed Testing, Inc. Glyphosate tolerant fescue grasses
JP2002520024A (ja) * 1998-07-10 2002-07-09 カルジーン エルエルシー 植物色素体における除草剤耐性遺伝子の発現
US6271444B1 (en) * 1998-07-10 2001-08-07 Calgene Llc Enhancer elements for increased translation in plant plastids
US6492578B1 (en) * 1998-07-10 2002-12-10 Calgene Llc Expression of herbicide tolerance genes in plant plastids
US8421158B2 (en) 1998-12-21 2013-04-16 Megica Corporation Chip structure with a passive device and method for forming the same
US8178435B2 (en) 1998-12-21 2012-05-15 Megica Corporation High performance system-on-chip inductor using post passivation process
RU2235778C2 (ru) * 1999-04-29 2004-09-10 Синджента Лимитед Выделенный полинуклеотид, способный придавать растению устойчивость или толерантность к глифосатному гербициду, вектор, способ получения растений, толерантных или устойчивых к глифосатному гербициду, способ регенерации трансформированного растения и способ селективной борьбы с сорняками
MXPA02006021A (es) 1999-12-16 2002-12-05 Monsanto Technology Llc Construcciones novedosas para expresion vegetal.
KR100381930B1 (ko) * 2000-03-16 2003-04-26 (주)바이오니아 유전자 변형 농산물 검출방법 및 검출용 프라이머
US6407319B1 (en) 2000-06-07 2002-06-18 Pure Seed Testing, Inc. Kentucky bluegrass variety known as ‘North Star’
US6372966B1 (en) 2000-06-07 2002-04-16 Pure Seed Testing Inc. Kentucky bluegrass variety known as ‘Brilliant’
EP1366070A2 (en) 2000-10-25 2003-12-03 Monsanto Technology LLC Cotton event pv-ghgt07(1445) and compositions and methods for detection thereof
AU2002218413A1 (en) * 2000-11-30 2002-06-11 Ses Europe N.V. Glyphosate resistant transgenic sugar beet characterised by a specific transgene insertion (t227-1), methods and primers for the detection of said insertion
US6423887B1 (en) 2001-03-14 2002-07-23 Pure Seed Testing, Inc. Glyphosate tolerant fescue grass variety
US7977046B2 (en) 2002-05-10 2011-07-12 Monsanto Technology Llc Method of selecting DNA constructs for herbicide tolerance in plants
SI1481068T1 (sl) 2002-02-26 2011-05-31 Syngenta Ltd Postopek za selektivno pripravo moških ali ženskih sterilnih rastlin
AU2003247962B2 (en) 2002-07-18 2008-06-12 Monsanto Technology Llc Methods for using artificial polynucleotides and compositions thereof to reduce transgene silencing
US7365186B2 (en) 2002-11-22 2008-04-29 Arborgen, Llc Vascular-preferred promoter sequences and uses thereof
FR2848570B1 (fr) 2002-12-12 2005-04-01 Bayer Cropscience Sa Cassette d'expression codant pour une 5-enol pyruvylshikimate-3-phosphate synthase (epsps) et plantes tolerantes aux herbicides la contenant
CA2743564C (en) 2003-01-13 2013-11-12 Monsanto Technology Llc Glyphosate tolerant alfalfa events and methods for detection thereof
AU2004252481B2 (en) 2003-06-06 2011-01-27 Arborgen Inc. Compositions and methods for regulating polysaccharides of a plant cell
US8153410B2 (en) 2003-07-07 2012-04-10 Fox Chase Cancer Center Alternate morpheein forms of allosteric proteins as a target for the development of bioactive molecules
US20060162014A1 (en) 2003-07-07 2006-07-20 Jaffe Eileen K Alternate morpheeins of allosteric proteins as a target for the development of bioactive molecules
DK2281895T3 (en) 2003-09-29 2018-04-16 Monsanto Technology Llc Methods for increasing stress tolerance in plants and compositions thereof
UA85690C2 (ru) 2003-11-07 2009-02-25 Басф Акциенгезелльшафт Смесь для применения в сельском хозяйстве, содержащая стробилурин и модулятор этилена, способ обработки и борьбы с инфекциями в бобовых культурах
CA2547563C (en) 2003-12-15 2014-02-18 Monsanto Technology Llc Corn plant mon88017 and compositions and methods for detection thereof
US7453025B2 (en) 2004-09-22 2008-11-18 Arborgen, Llc Reproductive ablation constructs
US7799906B1 (en) 2004-09-22 2010-09-21 Arborgen, Llc Compositions and methods for modulating lignin of a plant
US20070199095A1 (en) 2005-10-13 2007-08-23 Edwards Allen Methods for producing hybrid seed
PT1885176T (pt) 2005-05-27 2016-11-28 Monsanto Technology Llc Evento mon89788 de soja e métodos para a sua deteção
CN101278053A (zh) * 2005-07-29 2008-10-01 孟山都技术有限公司 利用来自转基因杂交体的分离子来开发新的种质
KR100674860B1 (ko) * 2005-08-10 2007-01-29 삼성전기주식회사 저온 소결용 글라스 프릿트
AR057205A1 (es) * 2005-12-01 2007-11-21 Athenix Corp Genes grg23 y grg51 que confieren resistencia a herbicidas
EP1984390A2 (en) 2006-01-23 2008-10-29 Board of Trustees of Michigan State University Methods for breeding glyphosate resistant plants and compositions thereof
WO2008002962A2 (en) * 2006-06-27 2008-01-03 Athenix Corporation Grg32: a novel epsp synthase gene conferring herbicide resistance
MX2009001573A (es) * 2006-08-11 2009-02-19 Monsanto Technology Llc Produccion de maiz alto en triptofano mediante la expresion dirigida al cloroplasto de la antranilato sintasa.
WO2009134785A1 (en) 2008-04-28 2009-11-05 Michigan Technological University Fiber-specific promoter elements
AR074941A1 (es) 2009-01-07 2011-02-23 Bayer Cropscience Sa Plantas transplastomicas exentas de marcador de seleccion
PL2445341T3 (pl) 2009-06-25 2018-01-31 Basf Se Zastosowanie mieszanin rolniczych zwiększających zdrowotność rośliny
US20120096600A1 (en) 2009-07-01 2012-04-19 Rene Ruiter Methods and means for obtaining plants with enhanced glyphosate tolerance
KR101868163B1 (ko) 2010-04-23 2018-06-15 히타치가세이가부시끼가이샤 ｐ 형 확산층 형성 조성물, ｐ 형 확산층의 제조 방법, 및 태양 전지 소자의 제조 방법
CN103037696A (zh) 2010-05-31 2013-04-10 巴斯夫欧洲公司 增加植物健康的方法
EP2726493B1 (en) 2011-07-01 2020-10-14 Monsanto Technology LLC Methods and compositions for selective regulation of protein expression
US9994863B2 (en) 2011-07-28 2018-06-12 Genective Glyphosate tolerant corn event VCO-O1981-5 and kit and method for detecting the same
AU2013205606B2 (en) * 2012-02-01 2015-05-21 Corteva Agriscience Llc Synthetic Brassica-derived chloroplast transit peptides
AU2013205557B2 (en) * 2012-04-17 2016-04-21 Corteva Agriscience Llc Synthetic brassica-derived chloroplast transit peptides
CA2894213A1 (en) 2012-12-21 2014-06-26 Pioneer Hi-Bred International, Inc. Compositions and methods for auxin-analog conjugation
AU2014236154A1 (en) 2013-03-14 2015-09-17 Pioneer Hi-Bred International, Inc. Compositions having dicamba decarboxylase activity and methods of use
BR112015023286A2 (pt) 2013-03-14 2018-03-06 Arzeda Corp polipeptídeo recombinante com atividade da dicamba descarboxilase, construto de polinucleotídeo, célula, método de produção de uma célula hospedeira compreendendo um polinucleotídeo heterólogo que codifica um polipeptídeo tendo atividade da dicamba descarboxilase, método para descarboxilar dicamba, um derivado de dicamba ou um metabolito de dicamba, método para a detecção de um polipeptideo e método para a detecção da presença de um polinucleotideo que codifica um polipeptideo tendo atividade da dicamba descarboxilase
CN114965840B (zh) * 2021-02-24 2024-06-14 公安部物证鉴定中心 一种生物体液中草甘膦、草铵膦及代谢物的检测方法
CN116411021B (zh) * 2023-06-07 2023-08-15 隆平生物技术(海南)有限公司 一种番茄草甘膦筛选体系的转化方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1170991A1 (ru) * 1982-06-16 1985-08-07 Всесоюзный Ордена Ленина И Ордена Трудового Красного Знамени Селекционно-Генетический Институт Способ селекции пшеницы,устойчивой к неблагопри тным факторам
US4535060A (en) * 1983-01-05 1985-08-13 Calgene, Inc. Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use
SU1165314A1 (ru) * 1983-03-28 1985-07-07 Институт экспериментальной ботаники им.В.Ф.Купревича Способ отбора устойчивых к 2,4- @ форм чмен
JP2615013B2 (ja) * 1985-08-07 1997-05-28 モンサント コンパニ− グリホセート耐性キメラ遺伝子
US4971908A (en) * 1987-05-26 1990-11-20 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthase
US5310667A (en) * 1989-07-17 1994-05-10 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases
EP0426641B1 (en) * 1989-10-31 2000-09-13 Monsanto Company Promoter for transgenic plants

Also Published As

Publication number Publication date
DE69120419T2 (de) 1997-01-09
KR920005467A (ko) 1992-03-28
EP0546090B2 (en) 2006-07-19
ATE139566T1 (de) 1996-07-15
DK0546090T3 (da) 1996-07-29
EP0546090A1 (en) 1993-06-16
RU2146290C1 (ru) 2000-03-10
TW232078B (es) 1994-10-11
JPH06500472A (ja) 1994-01-20
DE69120419T3 (de) 2007-02-15
DE69120419D1 (de) 1996-07-25
JP2001197841A (ja) 2001-07-24
UA32548C2 (uk) 2001-02-15
GR3021058T3 (en) 1996-12-31
ES2089232T5 (es) 2007-03-16
WO1992004449A1 (en) 1992-03-19
JP3173785B2 (ja) 2001-06-04
CA2088661A1 (en) 1992-03-01
AU655945B2 (en) 1995-01-19
CA2088661C (en) 2001-12-18
AU8657891A (en) 1992-03-30
EP0546090B1 (en) 1996-06-19
DK0546090T4 (da) 2006-11-20

Similar Documents

Publication Publication Date Title
ES2089232T3 (es) 5-enolpiruvilsiquimato-3-fosfato sintasas tolerantes del glifosato.
ES2173077T3 (es) Plantas que toleran glifosato.
Slade et al. An analysis of the costs and benefits of physiological integration between ramets in the clonal perennial herb Glechoma hederacea
Mosse et al. Vesicular-arbuscular mycorrhizal infections in root organ cultures
ES2039474T3 (es) Procedimineto mejorado para la transformacion de protoplastos vegetales.
ES2009605A6 (es) Metodo para producir delta-endotoxina de bacillus thuringiensis y metodo para producir dicha bacteria.
ES2061519T3 (es) Procedimiento para la produccion de acido eicosapentaenoico.
AR060750A1 (es) Metodos para producir cruzas fertiles entre especies de soja salvajes y domesticas
ES8202674A1 (es) Procedimiento de produccion de plantas micorrizadas por hon-gos simbioticos
ES2057246T3 (es) Dionas heterociclicas de utilidad como pesticidas y reguladores del crecimiento de las plantas.
AR020622A1 (es) Proceso para preparar cultivos lacticos iniciadores.
ES2063312T3 (es) Plantas transgenicas pertenecientes a la especie cucumis melo.
AR070309A1 (es) Un producto de tabaco obtenido de cultivar aob176 y metodo para obtener el producto
ES2024446B3 (es) Procedimiento para el incremento del crecimiento y la salud de plantas utiles.
CN204994390U (zh) 旋转式有机蔬菜无土栽培架
Jensen Decomposition of chlorine-substituted organic acids by fungi
ATE305718T1 (de) Neues verfahren zur herstellung und auswahl von transgenen flachspflanzen und leinsamen
Zhenhua et al. Effects of Water—Nitrogen Coupling on Nitrogen Uptake and Nitrate Accumulation in Soil of Oil Sunflower in Drip-irrigated Multiple Cropping System
ES8202363A1 (es) Procedimiento para la preparacion de derivados de oligorri- bosido de acido homocitrico
CN109258148A (zh) 一种蛇足石杉插条生根的方法
GT200000050A (es) Prodedimiento mejorado para fomentar el crecimiento de las plantas, vigorizando la flora demicroorganismos que se encuentran en la tierra.
GB8820835D0 (en) Plant breeding
ES8304201A1 (es) Un procedimiento para la preparacion de nuevas lactonas macrociclicas.
CN104255476A (zh) 一种节果决明悬浮细胞培养的快速繁殖方法
CN104206273A (zh) 一种黑三棱组织培养的快速繁殖方法

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 546090

Country of ref document: ES

FG2A Definitive protection

Ref document number: 546090

Country of ref document: ES