ES2091224T3 - Subtilisinas con mutaciones multiples. - Google Patents

Subtilisinas con mutaciones multiples.

Info

Publication number
ES2091224T3
ES2091224T3 ES90304715T ES90304715T ES2091224T3 ES 2091224 T3 ES2091224 T3 ES 2091224T3 ES 90304715 T ES90304715 T ES 90304715T ES 90304715 T ES90304715 T ES 90304715T ES 2091224 T3 ES2091224 T3 ES 2091224T3
Authority
ES
Spain
Prior art keywords
subtilisins
multiple mutations
subtilisin
admixture
expressing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90304715T
Other languages
English (en)
Spanish (es)
Inventor
Mark M Zukowski
Linda O Narhi
Michael Levitt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amgen Inc
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Application granted granted Critical
Publication of ES2091224T3 publication Critical patent/ES2091224T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cosmetics (AREA)
  • Tires In General (AREA)
ES90304715T 1989-05-17 1990-05-01 Subtilisinas con mutaciones multiples. Expired - Lifetime ES2091224T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US35312489A 1989-05-17 1989-05-17

Publications (1)

Publication Number Publication Date
ES2091224T3 true ES2091224T3 (es) 1996-11-01

Family

ID=23387862

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90304715T Expired - Lifetime ES2091224T3 (es) 1989-05-17 1990-05-01 Subtilisinas con mutaciones multiples.

Country Status (13)

Country Link
US (1) US5397705A (fr)
EP (1) EP0398539B1 (fr)
JP (1) JPH03505976A (fr)
KR (1) KR100188532B1 (fr)
AT (1) ATE141324T1 (fr)
AU (1) AU618675B2 (fr)
CA (1) CA2016211C (fr)
DE (1) DE69028040T2 (fr)
DK (1) DK0398539T3 (fr)
ES (1) ES2091224T3 (fr)
GR (1) GR3021010T3 (fr)
SG (1) SG47797A1 (fr)
WO (1) WO1990014420A1 (fr)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE371029T1 (de) * 1987-04-06 2007-09-15 Novozymes As Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
DK271490D0 (da) * 1990-11-14 1990-11-14 Novo Nordisk As Detergentkomposition
US5863783A (en) * 1991-03-27 1999-01-26 Gist-Brocades, N.V. Cloning and expression of DNA molecules encoding arabinan-degrading enzymes of fungal origin
WO1993008253A1 (fr) * 1991-10-16 1993-04-29 Unilever N.V. Compositions detergentes enzymatiques aqueuses
US5989887A (en) * 1992-11-25 1999-11-23 Dsm N.V. Cloning and expression of DNA molecules incoding arabinan-degrading enzymes of fungal origin
US5567601A (en) * 1993-06-01 1996-10-22 University Of Maryland Subtilisin mutants lacking a primary calcium binding site
US6440717B1 (en) 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
HU219851B (hu) * 1993-10-14 2001-08-28 The Procter And Gamble Company Proteáz tartalmú tisztítókészítmények
JP2888986B2 (ja) * 1993-10-14 1999-05-10 ザ、プロクター、エンド、ギャンブル、カンパニー プロテアーゼ酵素を含んでなる漂白組成物
MA23346A1 (fr) 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
DE4411223A1 (de) * 1994-03-31 1995-10-05 Solvay Enzymes Gmbh & Co Kg Verwendung alkalischer Proteasen in gewerblichen Textilwaschverfahren
US6599730B1 (en) 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6066611A (en) * 1994-10-13 2000-05-23 The Procter & Gamble Company Bleaching compositions comprising protease enzymes
US6455295B1 (en) 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
DE69720043T2 (de) * 1996-12-20 2003-10-16 Unilever N.V., Rotterdam Enzymatische bleichmittelzusammensetzung
US6204234B1 (en) * 1997-07-09 2001-03-20 The Proctor & Gamble Company Cleaning compositions comprising a specific oxygenase
ES2368718T3 (es) * 1997-10-23 2011-11-21 Danisco Us Inc. Variantes de subtilisina con múltiples sustituciones.
AR016969A1 (es) * 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
WO1999049056A1 (fr) * 1998-03-26 1999-09-30 The Procter & Gamble Company Variants de serine-proteases ayant des substitutions d'acides amines
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
CA2376045A1 (fr) 1999-07-22 2001-02-01 The Procter & Gamble Company Proteases variantes de la subtilisine par substitutions acide amine dans des regions definies de l'epitope
CZ2002171A3 (cs) 1999-07-22 2002-06-12 The Procter & Gamble Company Proteinázový konjugát, čistící prostředek a prostředek osobní péče
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
CA2379712A1 (fr) 1999-07-22 2001-02-01 The Procter & Gamble Company Variants de protease subtilisine possedant des suppressions et des substitutions d'acides amines dans certaines regions de sites antigeniques
EP2206786A1 (fr) * 1999-08-31 2010-07-14 Novozymes A/S Nouvelles protéases et variantes associées
US7217554B2 (en) 1999-08-31 2007-05-15 Novozymes A/S Proteases and variants thereof
US6727085B2 (en) * 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
WO2002010183A1 (fr) * 2000-07-31 2002-02-07 Menzel, Rolf Compositions et procedes d'assemblage dirige de genes
MXPA03007316A (es) 2001-02-19 2003-12-04 Merck Patent Gmbh Metodo para la identificacion de epitopes de celulas t y el uso para la preparacion de moleculas con inmunogenicidad reducida.
EP1373296B1 (fr) * 2001-03-23 2011-10-05 Genencor International, Inc. Proteines provoquant une reaction immunogene modifiee, et methodes de production et d'utilisation desdites proteines
CA2455607C (fr) * 2001-07-09 2013-01-08 Children's Hospital Medical Center Of Akron Temoins multiples pour analyses genetiques moleculaires
DE10153792A1 (de) 2001-10-31 2003-05-22 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
MXPA04006811A (es) * 2002-01-16 2004-10-11 Genencor Int Variantes de proteasa substituidas con multiplos.
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
US7294499B2 (en) 2003-01-30 2007-11-13 Novozymes A/S Subtilases
US7727756B2 (en) * 2003-03-21 2010-06-01 Novozymes A/S Subtilases
BRPI0409992A (pt) * 2003-05-07 2006-05-09 Novozymes As enzima subtilase, polinucleotìdeo isolado, construção de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, método para produzir a subtilase, composição limpadora ou detergente, usos de uma subtilase e de uma composição limpadora ou detergente, e, métodos para limpar ou lavar louças, lavar uma superfìcie dura ou lavar roupas e para remover manchas de ovo de uma superfìcie dura ou de roupas para lavar
EP3887515A1 (fr) * 2018-11-28 2021-10-06 Danisco US Inc. Variants de subtilisine à stabilité améliorée
CN119630280A (zh) 2022-05-14 2025-03-14 诺维信公司 用于预防、处理、抑制和/或消除植物病原性侵染和感染的组合物和方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5155033A (en) * 1989-01-06 1992-10-13 Genencor, Inc. Subtilisins modified at position 225 resulting in a shift in catalytic activity
WO1987004461A1 (fr) * 1986-01-15 1987-07-30 Amgen ANALOGUES DE SUBTILISINE THERMIQUEMENT STABLE ET STABLE AU pH, ET LEUR PROCEDE DE PRODUCTION
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
US5013657A (en) * 1988-04-12 1991-05-07 Bryan Philip N Subtilisin mutations
US4980288A (en) * 1986-02-12 1990-12-25 Genex Corporation Subtilisin with increased thermal stability
DE3750916T2 (de) * 1986-04-30 1995-06-01 Genencor Int Mutante einer nicht menschlichen Carbonyl-Hydrolase, für diese kodierende DNS-Sequenzen und Vektoren und durch diese Vektoren transformierte Wirte.
NZ221627A (en) * 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
ES2135386T3 (es) * 1987-02-27 1999-11-01 Genencor Int Transformacion de cepas de bacillus alcalofilas.
ATE371029T1 (de) * 1987-04-06 2007-09-15 Novozymes As Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
FR2614467A1 (fr) * 1987-04-21 1988-10-28 Telemecanique Electrique Appareil interrupteur de protection a mecanisme de declenchement simplifie

Also Published As

Publication number Publication date
ATE141324T1 (de) 1996-08-15
KR100188532B1 (ko) 1999-06-01
US5397705A (en) 1995-03-14
DE69028040D1 (de) 1996-09-19
HK1006857A1 (en) 1999-03-19
AU618675B2 (en) 1992-01-02
CA2016211C (fr) 1998-07-21
JPH03505976A (ja) 1991-12-26
DK0398539T3 (da) 1996-12-09
AU5558090A (en) 1990-12-18
GR3021010T3 (en) 1996-12-31
KR920701426A (ko) 1992-08-11
SG47797A1 (en) 1998-04-17
EP0398539A1 (fr) 1990-11-22
EP0398539B1 (fr) 1996-08-14
DE69028040T2 (de) 1997-02-27
CA2016211A1 (fr) 1990-11-17
WO1990014420A1 (fr) 1990-11-29

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