ES2097633T3 - Procedimiento mejorado para la ampliacion de fragmentos dirigidos de acido nucleico utilizando la reaccion en cadena de la polimerasa anidada. - Google Patents

Procedimiento mejorado para la ampliacion de fragmentos dirigidos de acido nucleico utilizando la reaccion en cadena de la polimerasa anidada.

Info

Publication number
ES2097633T3
ES2097633T3 ES94903404T ES94903404T ES2097633T3 ES 2097633 T3 ES2097633 T3 ES 2097633T3 ES 94903404 T ES94903404 T ES 94903404T ES 94903404 T ES94903404 T ES 94903404T ES 2097633 T3 ES2097633 T3 ES 2097633T3
Authority
ES
Spain
Prior art keywords
chain reaction
enlargement
nucleic acid
nested polymerase
improved procedure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94903404T
Other languages
English (en)
Inventor
Ron Grosz
Mark Anton Jensen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Application granted granted Critical
Publication of ES2097633T3 publication Critical patent/ES2097633T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

SE PRESENTA UN METODO MEJORADO PARA REALIZAR UNA AMPLIFICACION DE UNA REACCION DE CADENA DE POLIMERASA ANIDADA (PCR) DE UNA PIEZA OBJETIVADA DE DNA, EN EL QUE CONTROLADO LOS TIEMPOS DE RECOCIDO Y LA CONCENTRACION DEL CONJUNTO TANTO EXTERNO COMO INTERNO DE INICIADORES DE ACUERDO CON EL METODO PRESENTADO, PUEDE CONSEGUIRSE UNA AMPLIFICACION ALTAMENTE ESPECIFICA Y EFICIENTE DE UNA PIEZA OBJETIVADA DE DNA DENTRO DE UN VASO DE REACCION SIN EL AGOTAMIENTO O LA ELIMINACION DE LOS INICIADORES EXTERIORES DEL VASO DE LA MEZCLA DE REACCION.
ES94903404T 1992-12-09 1993-12-08 Procedimiento mejorado para la ampliacion de fragmentos dirigidos de acido nucleico utilizando la reaccion en cadena de la polimerasa anidada. Expired - Lifetime ES2097633T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/990,297 US5340728A (en) 1992-12-09 1992-12-09 Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction

Publications (1)

Publication Number Publication Date
ES2097633T3 true ES2097633T3 (es) 1997-04-01

Family

ID=25535998

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94903404T Expired - Lifetime ES2097633T3 (es) 1992-12-09 1993-12-08 Procedimiento mejorado para la ampliacion de fragmentos dirigidos de acido nucleico utilizando la reaccion en cadena de la polimerasa anidada.

Country Status (13)

Country Link
US (1) US5340728A (es)
EP (1) EP0675969B1 (es)
JP (1) JPH08504332A (es)
AT (1) ATE148925T1 (es)
AU (1) AU5736194A (es)
CA (1) CA2151036A1 (es)
DE (1) DE69308162T2 (es)
DK (1) DK0675969T3 (es)
ES (1) ES2097633T3 (es)
GR (1) GR3023325T3 (es)
IL (1) IL107935A0 (es)
MX (1) MX9307788A (es)
WO (1) WO1994013832A1 (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2189631A1 (es) * 2001-03-12 2003-07-01 Biotools Biotechnological & Me Metodo para la amplificacion de adn mediante semi-nested pcr en un solo paso y su empleo en la identificacion de especies de plasmodium.

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5817797A (en) * 1988-06-01 1998-10-06 The United States Of America As Represented By The Department Of Health And Human Services Sequencing DNA; a modification of the polymerase chain reaction
US5912121A (en) * 1992-12-11 1999-06-15 Bringham And Women's Hospital Methods for detecting mutations associated with hypertrophic cardiomyopathy
US20020127548A1 (en) * 1992-12-11 2002-09-12 Christine Siedman Methods for detecting mutations associated with hypertrophic cardiomyopathy
US5556773A (en) * 1993-08-06 1996-09-17 Yourno; Joseph Method and apparatus for nested polymerase chain reaction (PCR) with single closed reaction tubes
US5928905A (en) * 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US5834252A (en) * 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5660981A (en) 1994-06-06 1997-08-26 E. I. Du Pont De Nemours And Company Selection of diagnostic genetic markers in microorganisms and use of a specific marker for detection of salmonella
US5559013A (en) * 1994-06-22 1996-09-24 Johnson & Johnson Clinical Diagnostics, Inc. Method of amplification using intermediate renaturation step
US5705366A (en) * 1994-09-15 1998-01-06 Johnson & Johnson Clinical Diagnostics, Inc. Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
NZ296748A (en) 1994-11-02 1999-11-29 Univ New York State Res Found Peripheral nervous system specific sodium channel proteins, DNA coding therefor, compositions and methods
US6110665A (en) * 1995-02-14 2000-08-29 University Of Kentucky Research Foundation Sarcocystis neuronadiagnostic primer and its use in methods of equine protozoal myeloencephalitis diagnosis
GB9509112D0 (en) * 1995-05-04 1995-06-28 Scottish Crop Research Inst Polynucleotide
GB9509410D0 (en) * 1995-05-10 1995-07-05 Imperial College Molecular imaging
US5693467A (en) * 1995-05-19 1997-12-02 The American Type Culture Collection Mycoplasma polymerase chain reaction testing system using a set of mixed and single sequence primers
WO1996036736A2 (en) * 1995-05-19 1996-11-21 Abbott Laboratories Wide dynamic range nucleic acid detection using an aggregate primer series
CA2223061C (en) * 1995-06-06 2008-04-22 Jan Vijg Method of and apparatus for diagnostic dna testing
US5882857A (en) * 1995-06-07 1999-03-16 Behringwerke Ag Internal positive controls for nucleic acid amplification
WO1996040992A2 (en) * 1995-06-07 1996-12-19 Abbott Laboratories Probe masking method of reducing background in an amplification reaction
AT402203B (de) 1995-06-13 1997-03-25 Himmler Gottfried Dipl Ing Dr Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren
DK0785281T3 (da) * 1995-07-28 2004-09-06 Sapporo Breweries Fremgangsmåde til genetisk differentiering af humlesort
US5747257A (en) * 1996-02-29 1998-05-05 E. I. Du Pont De Nemours And Company Genetic markers and methods for the detection of escherichia coli serotype-0157:H7
US6458530B1 (en) * 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
ATE428801T1 (de) * 1996-06-04 2009-05-15 Univ Utah Res Found Überwachung der hybridisierung während pcr
US5989873A (en) * 1996-09-24 1999-11-23 Vinayagamoorthy; Thuraiayah Method of detecting gene expression and/or of preventing such expression in cells
US5922538A (en) * 1996-11-08 1999-07-13 E.I. Du Pont De Nemours And Company Genetic markers and methods for the detection of Listeria monocytogenes and Listeria spp
IL120339A0 (en) * 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Improved DNA assembly method
US6103468A (en) * 1997-10-07 2000-08-15 Labatt Brewing Company Limited Rapid two-stage polymerase chain reaction method for detection of lactic acid bacteria in beer
US6468743B1 (en) * 1998-05-18 2002-10-22 Conagra Grocery Products Company PCR techniques for detecting microbial contaminants in foodstuffs
US6232104B1 (en) 1999-08-17 2001-05-15 Dade Behring Inc. Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration
JP3979024B2 (ja) * 2001-03-29 2007-09-19 日本製紙株式会社 Ｒａｐｄ法による個体識別方法
NZ530343A (en) * 2001-06-22 2007-01-26 Marshfield Clinic Methods and oligonucleotides for the detection of E. coli 0157:H7
US7273730B2 (en) * 2002-05-24 2007-09-25 Invitrogen Corporation Nested PCR employing degradable primers
EP3000899A1 (en) 2002-12-04 2016-03-30 Applied Biosystems, LLC Multiplex amplification of polynucleotides
US20060024690A1 (en) * 2003-09-19 2006-02-02 Kao H P Normalization of data using controls
WO2005029041A2 (en) * 2003-09-19 2005-03-31 Applera Corporation High density sequence detection methods and apparatus
NZ546903A (en) * 2003-10-13 2009-06-26 Genaco Biomedical Products Inc Method and kit for primer based amplification of nucleic acids
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
US20060003337A1 (en) * 2004-06-30 2006-01-05 John Brandis Detection of small RNAS
KR100671501B1 (ko) * 2005-02-28 2007-01-19 삼성에버랜드 주식회사 식중독 검출용 프라이머 및 이를 이용한 세균성 식중독 검출방법
US20060240442A1 (en) * 2005-04-20 2006-10-26 Vevea Dirk N Methods and oligonucleotides for the detection of Salmonella SP., E coli 0157:H7, and Listeria monocytogenes
EP3167900B1 (en) 2006-03-29 2018-11-21 Merial Limited Vaccine against streptococci
FR2926367B1 (fr) * 2008-01-10 2013-01-04 Novaleads Procede fluorimetrique pour evaluer l'influence d'une condition sur un echantillon biologique et ses applications.
CA2764315A1 (en) * 2009-06-02 2010-12-09 Monoquant Pty Ltd A method of nucleic acid amplification
EP2776576B1 (en) * 2011-11-09 2018-03-21 Qualicon Diagnostics LLC Sequences and their use for detection of salmonella
US20230366038A1 (en) * 2022-05-16 2023-11-16 Bioo Scientific Corporation Compositions and methods relating to loop mediated isothermal amplification (lamp)

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
JPH0638759B2 (ja) * 1988-07-29 1994-05-25 株式会社島津製作所 サルモネラ菌検査に用いるｄｎａプローブ及びそれを用いたサルモネラ菌の検出法
DE69031318D1 (de) * 1989-03-17 1997-10-02 Abbott Lab Verfahren und Vorrichtung zur Hybridisierung von Nukleinsäure mit verbesserter Reaktionskinetik
ES2045932T3 (es) * 1989-06-12 1994-01-16 Cis Bio Int Procedimiento para la deteccion de secuencias especificas de acidos nucleicos y sus aplicaciones.
DE69032778T2 (de) * 1989-07-18 1999-07-29 Shimadzu Corp., Kyoto Verfahren zur Untersuchung von durch Mikroorganismen verursachten Lebensmittelvergiftungen und Reagens dafür
US5126239A (en) * 1990-03-14 1992-06-30 E. I. Du Pont De Nemours And Company Process for detecting polymorphisms on the basis of nucleotide differences
JPH0491790A (ja) * 1990-08-02 1992-03-25 Shionogi & Co Ltd ２段階ｐｃｒ法
GB9107124D0 (en) * 1991-04-05 1991-05-22 Dynal As Chemical process

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2189631A1 (es) * 2001-03-12 2003-07-01 Biotools Biotechnological & Me Metodo para la amplificacion de adn mediante semi-nested pcr en un solo paso y su empleo en la identificacion de especies de plasmodium.
ES2189631B1 (es) * 2001-03-12 2005-01-01 BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. Metodo para la amplificacion de adn mediante semi-nested pcr en un solo paso y su empleo en la identificacion de especies de plasmodium.

Also Published As

Publication number Publication date
CA2151036A1 (en) 1994-06-23
JPH08504332A (ja) 1996-05-14
DE69308162D1 (de) 1997-03-27
US5340728A (en) 1994-08-23
AU5736194A (en) 1994-07-04
EP0675969A1 (en) 1995-10-11
EP0675969B1 (en) 1997-02-12
DK0675969T3 (da) 1997-04-28
WO1994013832A1 (en) 1994-06-23
ATE148925T1 (de) 1997-02-15
GR3023325T3 (en) 1997-08-29
MX9307788A (es) 1994-07-29
DE69308162T2 (de) 1997-06-26
IL107935A0 (en) 1994-04-12

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