ES2099052T1 - Agentes de solubilizacion de biomoleculas constituidos por iones hibridos no detergentes y su utilizacion. - Google Patents

Agentes de solubilizacion de biomoleculas constituidos por iones hibridos no detergentes y su utilizacion.

Info

Publication number
ES2099052T1
ES2099052T1 ES95907027T ES95907027T ES2099052T1 ES 2099052 T1 ES2099052 T1 ES 2099052T1 ES 95907027 T ES95907027 T ES 95907027T ES 95907027 T ES95907027 T ES 95907027T ES 2099052 T1 ES2099052 T1 ES 2099052T1
Authority
ES
Spain
Prior art keywords
agents
sup
biomolecules
constituted
solomilization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
ES95907027T
Other languages
English (en)
Inventor
Laurent Vuillard
Thierry Rabilloud
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LAUE MAX INST
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Original Assignee
LAUE MAX INST
Commissariat a lEnergie Atomique CEA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by LAUE MAX INST, Commissariat a lEnergie Atomique CEA filed Critical LAUE MAX INST
Publication of ES2099052T1 publication Critical patent/ES2099052T1/es
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D213/00—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members
    • C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
    • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D213/06—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom containing only hydrogen and carbon atoms in addition to the ring nitrogen atom
    • C07D213/16—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom containing only hydrogen and carbon atoms in addition to the ring nitrogen atom containing only one pyridine ring
    • C07D213/20—Quaternary compounds thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C309/00—Sulfonic acids; Halides, esters, or anhydrides thereof
    • C07C309/01—Sulfonic acids
    • C07C309/02—Sulfonic acids having sulfo groups bound to acyclic carbon atoms
    • C07C309/03—Sulfonic acids having sulfo groups bound to acyclic carbon atoms of an acyclic saturated carbon skeleton
    • C07C309/13—Sulfonic acids having sulfo groups bound to acyclic carbon atoms of an acyclic saturated carbon skeleton containing nitrogen atoms, not being part of nitro or nitroso groups, bound to the carbon skeleton
    • C07C309/14—Sulfonic acids having sulfo groups bound to acyclic carbon atoms of an acyclic saturated carbon skeleton containing nitrogen atoms, not being part of nitro or nitroso groups, bound to the carbon skeleton containing amino groups bound to the carbon skeleton
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D295/00—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms
    • C07D295/04—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms with substituted hydrocarbon radicals attached to ring nitrogen atoms
    • C07D295/08—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms with substituted hydrocarbon radicals attached to ring nitrogen atoms substituted by singly bound oxygen or sulfur atoms
    • C07D295/084—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms with substituted hydrocarbon radicals attached to ring nitrogen atoms substituted by singly bound oxygen or sulfur atoms with the ring nitrogen atoms and the oxygen or sulfur atoms attached to the same carbon chain, which is not interrupted by carbocyclic rings
    • C07D295/088—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms with substituted hydrocarbon radicals attached to ring nitrogen atoms substituted by singly bound oxygen or sulfur atoms with the ring nitrogen atoms and the oxygen or sulfur atoms attached to the same carbon chain, which is not interrupted by carbocyclic rings to an acyclic saturated chain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C2601/00—Systems containing only non-condensed rings
    • C07C2601/12—Systems containing only non-condensed rings with a six-membered ring
    • C07C2601/14—The ring being saturated

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Pyridine Compounds (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Pyrrole Compounds (AREA)

Abstract

LA INVENCION SE REFIERE A AGENTES DE SOLUBILIZACION DE BIOMOLECULAS, EN PARTICULAR DE PROTEINAS. ESTOS AGENTES ESTAN CONSTITUIDOS POR IONES ANFOTERICOS QUE RESPONDEN A UNA DE LAS FORMULAS (I) O (II) EN LAS QUE : X ES N O P, B ES UNA CADENA HIDROCARBONADA DE 1 A 6 ATOMOS DE CARBONO, R{SUP,1} R{SUP,2} Y R{SUP,3} SON GRUPO HIDROCARBONADOS, R{SUP,1} Y R{SUP,2} PUEDEN FORMAR CON X UN HETEROCICLO, R REPRESENTA CON X UN HETEROCICLO INSATURADO, E Y ES SO{SUB,3}-, OSO{SUB,3}- O COO-: ESTOS AGENTES PUEDEN SER UTILIZADOS EN PROCEDIMIENTOS DE PURIFICACION, DE SOLUBILIZACION O DE ANALISIS DE PROTEINAS, EN PARTICULAR POR ELECTROFORESIS O CROMATOGRAFIA.
ES95907027T 1994-01-12 1995-01-11 Agentes de solubilizacion de biomoleculas constituidos por iones hibridos no detergentes y su utilizacion. Pending ES2099052T1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9400241A FR2714906B1 (fr) 1994-01-12 1994-01-12 Agents de solubilisation de biomolécules constitués par des zwitterions non détergents et leurs utilisations.

Publications (1)

Publication Number Publication Date
ES2099052T1 true ES2099052T1 (es) 1997-05-16

Family

ID=9458941

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95907027T Pending ES2099052T1 (es) 1994-01-12 1995-01-11 Agentes de solubilizacion de biomoleculas constituidos por iones hibridos no detergentes y su utilizacion.

Country Status (6)

Country Link
EP (1) EP0739334A1 (es)
JP (1) JPH09511735A (es)
DE (1) DE739334T1 (es)
ES (1) ES2099052T1 (es)
FR (1) FR2714906B1 (es)
WO (1) WO1995019339A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPO403496A0 (en) * 1996-12-05 1997-01-02 Macquarie Research Limited Electrophoresis separation methods
US20020052026A1 (en) 1997-10-08 2002-05-02 Steven M. Vicik Methods of refolding proteins

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4372888A (en) * 1980-08-26 1983-02-08 The United States Of America As Represented By The Secretary Of The Department Of Health & Human Services Nondenaturing zwitterionic detergents
EP0141879A1 (en) * 1983-10-18 1985-05-22 Victor A. Bernstam Surfactant compositions and methods for clarifying and partitioning aqueous lipid-containing specimens
CA2048302A1 (en) * 1990-08-15 1992-02-16 Victoria P. Meucci Solubilization reagent for biological test samples
JPH0557114A (ja) * 1991-01-11 1993-03-09 Millipore Corp 高効率の毛管電気泳動分離法

Also Published As

Publication number Publication date
JPH09511735A (ja) 1997-11-25
WO1995019339A1 (fr) 1995-07-20
EP0739334A1 (fr) 1996-10-30
DE739334T1 (de) 1997-06-05
FR2714906B1 (fr) 1996-02-02
FR2714906A1 (fr) 1995-07-13

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