ES2172801T3 - Procedimiento y dispositivo para la purificacion y enriquecimiento de moleculas. - Google Patents

Procedimiento y dispositivo para la purificacion y enriquecimiento de moleculas.

Info

Publication number
ES2172801T3
ES2172801T3 ES97931688T ES97931688T ES2172801T3 ES 2172801 T3 ES2172801 T3 ES 2172801T3 ES 97931688 T ES97931688 T ES 97931688T ES 97931688 T ES97931688 T ES 97931688T ES 2172801 T3 ES2172801 T3 ES 2172801T3
Authority
ES
Spain
Prior art keywords
molecules
enrichment
purification
procedure
dissolution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES97931688T
Other languages
English (en)
Inventor
Wolf M Prof Dr Bertling
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin
Original Assignee
November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19628171A external-priority patent/DE19628171C2/de
Application filed by November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin filed Critical November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin
Application granted granted Critical
Publication of ES2172801T3 publication Critical patent/ES2172801T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Electrochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Electrostatic Separation (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

LA INVENCION TRATA DE UN PROCEDIMIENTO PARA LA PURIFICACION Y ENRIQUECIMIENTO DE PRIMERAS MOLECULAS (9) QUE ESTAN CARGADAS, COMO PROTEINAS, ACIDOS NUCLEICOS Y SIMILARES, QUE COMPRENDE LAS SIGUIENTES ETAPAS: A) PREPARACION DE UNA DISOLUCION QUE CONTENGA LAS PRIMERAS MOLECULAS (9), B) PONER EN CONTACTO LA DISOLUCION CON UN ELECTRODO (2), EL CUAL LLEVA DIRECTAMENTE UNA CAPA QUE TIENE UNA AFINIDAD CON LAS SEGUNDAS MOLECULAS (4) QUE CONTIENEN LAS PRIMERAS MOLECULAS (9), Y C) UNIR EL ELECTRODO (2) CON UN MEDIO (11) PARA FORMAR UN CAMPO ELECTRICO, DE TAL MANERA QUE OCURRA EN LA DISOLUCION UN MOVIMIENTO DE LAS PRIMERAS MOLECULAS (9) DIRIGIDA HACIA EL ELECTRODO (2).
ES97931688T 1996-07-12 1997-06-28 Procedimiento y dispositivo para la purificacion y enriquecimiento de moleculas. Expired - Lifetime ES2172801T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19628171A DE19628171C2 (de) 1996-07-12 1996-07-12 Verfahren und Vorrichtung zur Aufreinigung und Anreicherung von Molekülen
DE29702254U DE29702254U1 (de) 1996-07-12 1997-02-10 Vorrichtung zur Aufreinigung von Molekülen

Publications (1)

Publication Number Publication Date
ES2172801T3 true ES2172801T3 (es) 2002-10-01

Family

ID=26027454

Family Applications (1)

Application Number Title Priority Date Filing Date
ES97931688T Expired - Lifetime ES2172801T3 (es) 1996-07-12 1997-06-28 Procedimiento y dispositivo para la purificacion y enriquecimiento de moleculas.

Country Status (8)

Country Link
US (1) US6479644B1 (es)
EP (1) EP0912470B9 (es)
JP (1) JP2000515508A (es)
AT (1) ATE213227T1 (es)
AU (1) AU719533B2 (es)
CA (1) CA2260132A1 (es)
ES (1) ES2172801T3 (es)
WO (1) WO1998002399A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL139393A0 (en) * 1998-05-04 2001-11-25 Fraunhofer Ges Forschung Method and device for isolating nucleic acids
US6699698B1 (en) 1999-02-15 2004-03-02 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Method and sample mount system for the in situ separation and enrichment of substances
DE19940647C2 (de) * 1999-08-26 2002-11-28 November Ag Molekulare Medizin Verfahren zum Nachweis und zur Quantifizierung von in einer Flüssigkeit befindlichen ersten Biopolymeren
DE10025174A1 (de) * 2000-05-24 2001-12-06 November Ag Molekulare Medizin Verfahren zur Herstellung einer mit Biomolekülen beschichteten Elektrode
DE10211741B4 (de) * 2002-03-14 2005-12-01 November Ag Verfahren zum elektrochemischen Detektieren eines Analyten
US20060281168A1 (en) 2005-06-13 2006-12-14 Tosoh Corporation Cell fusion chamber, cell fusion device, and method for cell fusion using the same
EP1931979B1 (en) 2005-10-04 2015-11-18 Headway Technologies, Inc. Microfluidic detection of analytes
JP2009273459A (ja) * 2008-04-15 2009-11-26 Tosoh Corp 細胞選別装置、およびそれを用いた細胞選別方法
CN103900894A (zh) * 2008-06-06 2014-07-02 华盛顿大学 用于从溶液浓缩颗粒的方法和系统
US8940092B1 (en) 2008-10-27 2015-01-27 University Of Washington Through Its Center For Commercialization Hybrid fibers, devices using hybrid fibers, and methods for making hybrid fibers

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1152449A (en) 1979-12-31 1983-08-23 The Regents Of The University Of California Method and apparatus for dynamic equilibrium electrophoresis
US4545888A (en) 1984-04-06 1985-10-08 Walsh J William Apparatus for electrophoretic recovery of nucleic acids and other substances
FR2574311B1 (fr) * 1984-12-11 1989-09-15 Inst Nat Sante Rech Med Perfectionnements aux procedes et appareils d'electrophorese preparative
JPS62184343A (ja) * 1986-02-07 1987-08-12 Fuji Photo Film Co Ltd 電気泳動装置
JPS63317756A (ja) * 1987-06-19 1988-12-26 Fuji Photo Film Co Ltd 電気泳動装置
GB8729002D0 (en) 1987-12-11 1988-01-27 Iq Bio Ltd Electrode material
AT390274B (de) * 1988-03-15 1990-04-10 Steininger Karl Heinz Elektrode
NL8801073A (nl) * 1988-04-26 1989-11-16 Univ Twente Detectiemethode.
GB8905454D0 (en) 1989-03-10 1989-04-19 Mainwaring Robert Altenator tester
US5196099A (en) 1990-04-13 1993-03-23 W. R. Grace & Co.-Conn. Electrophoretic matrix and electrophoresis using same
US5776672A (en) * 1990-09-28 1998-07-07 Kabushiki Kaisha Toshiba Gene detection method
US5217593A (en) 1991-03-13 1993-06-08 Macconnell William P Nucleic acid purification system and method
US6017696A (en) * 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
JPH05236997A (ja) * 1992-02-28 1993-09-17 Hitachi Ltd ポリヌクレオチド捕捉用チップ
FR2687931A1 (fr) 1992-02-28 1993-09-03 Centre Nat Rech Scient Procedes d'electrophorese et d'optimisation d'electrophorese et appareil pour leur mise en óoeuvre.
WO1994025144A1 (en) 1993-05-05 1994-11-10 Amrad Corporation Limited Electrophoretic resolution of charged molecules
US5871918A (en) * 1996-06-20 1999-02-16 The University Of North Carolina At Chapel Hill Electrochemical detection of nucleic acid hybridization
US5968745A (en) * 1995-06-27 1999-10-19 The University Of North Carolina At Chapel Hill Polymer-electrodes for detecting nucleic acid hybridization and method of use thereof

Also Published As

Publication number Publication date
AU3537697A (en) 1998-02-09
EP0912470B1 (de) 2002-02-13
EP0912470B9 (de) 2002-09-25
AU719533B2 (en) 2000-05-11
CA2260132A1 (en) 1998-01-22
US6479644B1 (en) 2002-11-12
JP2000515508A (ja) 2000-11-21
EP0912470A1 (de) 1999-05-06
WO1998002399A1 (de) 1998-01-22
ATE213227T1 (de) 2002-02-15

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