ES2100955T3 - Metodo para deteccion microbiana rapida. - Google Patents

Metodo para deteccion microbiana rapida.

Info

Publication number
ES2100955T3
ES2100955T3 ES91914850T ES91914850T ES2100955T3 ES 2100955 T3 ES2100955 T3 ES 2100955T3 ES 91914850 T ES91914850 T ES 91914850T ES 91914850 T ES91914850 T ES 91914850T ES 2100955 T3 ES2100955 T3 ES 2100955T3
Authority
ES
Spain
Prior art keywords
bacteria
bacteriophage
sample
detection method
infect
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91914850T
Other languages
English (en)
Inventor
Catherine Elizabeth Dunn Rees
Katalin Rostas-Mulligan
Simon Fearon Park
Stephen Paul Denyer
Gordon Sydney Anderson Stewart
Sabah Abdel Amir Jassim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Patent GmbH
Original Assignee
Merck Patent GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent GmbH filed Critical Merck Patent GmbH
Application granted granted Critical
Publication of ES2100955T3 publication Critical patent/ES2100955T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C12N15/03—Bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

UN METODO PARA ANALIZAR UNA BACTERIA DE BLANCO QUE CONSISTE EN AÑADIR UN BACTERIOLAGO A UNA MUESTRA PARA INFECTAR LA BACTERIA QUE SE ENCUENTRA EN LA MUESTRA; MATAR EL BACTERIOLAGO EXTRACELULAR SIN MATAR AL MISMO TIEMPO LA BACTERIA INFECTADA CON EL LAGO; AMPLIFICAR EL BACTERIOLAGO QUE QUEDA EN LA MUESTRA; Y HACER QUE EL BACTERIOLAGO INFECTE UNA BACTERIA REPORTERA Y ASI PRODUCIR UNA SEÑAL VISIBLE. LAS BACTERIAS REPORTERAS SON SOMETIDAS A UNA INGENIERIA GENETICA PARA TENER UN GEN INDICADOR QUE AL EXPRESARSE DA LUGAR A UNA SEÑAL DETECTABLE EN DONDE LA EXPRESION DEL GEN INDICADOR SE INICIA EN UNA INFECCION DEL BACTERIOLAGO DE LA BACTERIA.
ES91914850T 1990-08-09 1991-08-08 Metodo para deteccion microbiana rapida. Expired - Lifetime ES2100955T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB909017443A GB9017443D0 (en) 1990-08-09 1990-08-09 Reporter bacteria for rapid microbial detection

Publications (1)

Publication Number Publication Date
ES2100955T3 true ES2100955T3 (es) 1997-07-01

Family

ID=10680380

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91914850T Expired - Lifetime ES2100955T3 (es) 1990-08-09 1991-08-08 Metodo para deteccion microbiana rapida.

Country Status (10)

Country Link
US (2) US5498525A (es)
EP (1) EP0495960B1 (es)
JP (1) JP3088014B2 (es)
AT (1) ATE150489T1 (es)
CA (1) CA2066561C (es)
DE (1) DE69125263T2 (es)
DK (1) DK0495960T3 (es)
ES (1) ES2100955T3 (es)
GB (1) GB9017443D0 (es)
WO (1) WO1992002633A1 (es)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9317139D0 (en) * 1993-08-18 1993-10-06 Miniser For Agriculture Fisher Method and test kits for detection of bacteriophage
GB2295893B (en) * 1993-08-18 1997-10-08 Mini Agriculture & Fisheries Method and test kits for detection of bacteriophage
BR9506962A (pt) * 1994-03-01 1997-09-16 Merck Patent Gmbh Cultura seletiva de virus
DE19517940A1 (de) * 1995-05-18 1996-11-21 Merck Patent Gmbh Nachweis von Listerien mittels rekombinanter Bakteriophagen
US6461833B1 (en) 1995-12-15 2002-10-08 Biotec Laboratories Limited Method to detect bacteria
GB9525661D0 (en) * 1995-12-15 1996-02-14 Biotec Diagnostics Limited Method
US6391578B2 (en) 1997-04-09 2002-05-21 3M Innovative Properties Company Method and devices for partitioning biological sample liquids into microvolumes
US6696286B1 (en) 1997-04-09 2004-02-24 3M Innovative Properties Company Method and devices for detecting and enumerating microorganisms
US5958675A (en) * 1997-04-18 1999-09-28 3M Innovative Properties Company Method for detecting bacteria using bacteriophage, contrast-coloring dye and precipitable dye
AU4840899A (en) 1998-06-29 2000-01-17 Procter & Gamble Company, The Disposable article having proactive sensor
US7132247B1 (en) 1998-09-17 2006-11-07 Regents Of The University Of Minnesota Composite devices incorporating biological material and methods
US6174699B1 (en) 1999-03-09 2001-01-16 3M Innovative Properties Company Disc assay device with inoculation pad and methods of use
US6501002B1 (en) 1999-06-29 2002-12-31 The Proctor & Gamble Company Disposable surface wipe article having a waste contamination sensor
US6391626B1 (en) 1999-08-23 2002-05-21 3M Innovative Properties Company Culture medium and device using ballasted pH indicators for detection and enumeration of microorganisms
US6737266B1 (en) * 1999-10-01 2004-05-18 3M Innovative Properties Company Devices and methods for microorganism detection
US6436661B1 (en) * 2000-04-13 2002-08-20 3M Innovative Properties Company Bacteria and bacteriophage detection using immobilized enzyme substrates
US20020192676A1 (en) * 2001-06-18 2002-12-19 Madonna Angelo J. Method for determining if a type of bacteria is present in a mixture
CA2474159A1 (en) * 2002-01-23 2003-05-21 Carl R. Merril Method for determining sensitivity to a bacteriophage
US20050003346A1 (en) * 2002-04-12 2005-01-06 Colorado School Of Mines Apparatus and method for detecting microscopic living organisms using bacteriophage
US8216780B2 (en) 2002-04-12 2012-07-10 Microphage (Tm) Incorporated Method for enhanced sensitivity in bacteriophage-based diagnostic assays
JP4490112B2 (ja) * 2002-04-12 2010-06-23 コロラド・スクール・オブ・マインズ 標的細菌性細胞を感染させるためにファージを使用する低濃度の標的細菌を検出するための方法
EP1613965A2 (en) 2003-04-10 2006-01-11 Kent J. Voorhees Apparatus and method for detecting microscopic living organisms using bacteriophage
WO2005014805A1 (en) * 2003-08-08 2005-02-17 Regents Of The University Of Minnesota A structured material for the production of hydrogen
US20090286225A1 (en) * 2004-02-13 2009-11-19 Microphagetm Incorporated Method and apparatus for bacteriophage-based diagnostic assays
WO2008157384A2 (en) 2007-06-15 2008-12-24 Microphage Incorporated Method of detection of microorganisms with enhanced bacteriophage amplification
US20050250096A1 (en) * 2004-02-13 2005-11-10 Microphage, Incorporated Microorganism detection using bacteriophage amplification
PL1812585T3 (pl) 2004-11-01 2015-03-31 Erber Ag Bakteriofagi jako czynniki selekcyjne
US20110097702A1 (en) * 2005-03-31 2011-04-28 Voorhees Kent J Methods and compositions for in situ detection of microorganisms on a surface
WO2006105504A1 (en) * 2005-03-31 2006-10-05 Microphage Incorporated Apparatus and method for detecting microorganisms using flagged bacteriophage
EP1885853A2 (en) * 2005-05-27 2008-02-13 Guild Associates, Inc. Biological detection system and method
US20070178450A1 (en) * 2006-01-27 2007-08-02 Microphage (Tm) Incorporation Method and apparatus for determining level of microorganisms using bacteriophage
WO2008063838A2 (en) * 2006-10-31 2008-05-29 Microphage Incorporated Method and apparatus for enhanced bacteriophage-based diagnostic assays by selective inhibition of potential cross-reactive organisms
US9212383B2 (en) * 2007-04-04 2015-12-15 Guild Associates, Inc. Biological detection system and method
CN101329342A (zh) * 2007-06-22 2008-12-24 王霁 检测结核菌和抗结核药物敏感性的方法及试剂盒
US8697434B2 (en) * 2008-01-11 2014-04-15 Colorado School Of Mines Detection of phage amplification by SERS nanoparticles
US9441204B2 (en) * 2008-04-03 2016-09-13 Colorado School Of Mines Compositions and methods for detecting Yersinia pestis bacteria
US20100112630A1 (en) * 2008-11-03 2010-05-06 Scott Martell Boyette Methods for measuring microbiological content in aqueous media
US8481302B2 (en) * 2008-11-03 2013-07-09 General Electric Company Total bacteria monitoring system
US20110183314A1 (en) * 2010-01-26 2011-07-28 Microphage Incorporated Bacteriophage-based microorganism diagnostic assay using speed or acceleration of bacteriophage reproduction
GB2477752A (en) 2010-02-11 2011-08-17 Arab Biotechnology Company Detection of bacteria
US9481903B2 (en) 2013-03-13 2016-11-01 Roche Molecular Systems, Inc. Systems and methods for detection of cells using engineered transduction particles
DK2968424T3 (da) 2013-03-13 2020-03-30 Geneweave Biosciences Inc Ikke-replikative transduktionspartikler og transduktionspartikelbaserede reportersystemer
US8945837B2 (en) 2013-03-15 2015-02-03 American Sterilizer Company Sterilization indicator including a simplified genetically engineered biological indicator
WO2014150870A2 (en) 2013-03-15 2014-09-25 Altria Client Services Inc. Bacteriophage and methods of making and using
JP2016517687A (ja) 2013-03-27 2016-06-20 サンプル テクノロジーズ，インコーポレイティド 組換えファージ及び細菌検出方法
AU2014281406A1 (en) 2013-06-19 2016-01-21 Sample6 Technologies, Inc. Phage-based bacterial detection assay
ES2731325T3 (es) 2013-10-01 2019-11-15 Univ Nottingham Detección de micobacterias usando bacteriófagos
US9540675B2 (en) 2013-10-29 2017-01-10 GeneWeave Biosciences, Inc. Reagent cartridge and methods for detection of cells
US9781929B2 (en) 2015-01-29 2017-10-10 Altria Client Services Llc Bacteriophage and methods of using
US10357056B2 (en) 2015-01-29 2019-07-23 Altria Client Services Llc Endolysin from bacteriophage against Geobacillus and methods of using
US10351893B2 (en) 2015-10-05 2019-07-16 GeneWeave Biosciences, Inc. Reagent cartridge for detection of cells

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1277931C (en) * 1984-06-05 1990-12-18 Shimon Ulitzur Detection and/or identification of microorganisms in a test sample usingbioluminescence or other exogenous genetically-introduced marker
FR2621924B1 (fr) * 1987-10-19 1990-01-12 Pasteur Institut Gene recombinant, notamment retrovirus recombinant defectif et activable par un transactivateur, cellules les contenant, leur procede de fabrication et leurs applications, notamment pour la detection in vitro d'une infection retrovirale ou de l'etude de l'inhibition in vitro de l'infection par des principes actifs de medicaments
DE3833628A1 (de) * 1988-10-03 1990-04-12 Genlux Forschungsgesellschaft Verfahren zum nachweis und zur identifikation toxischer substanzen mit hilfe klonierter mikroorganismen
JP2944694B2 (ja) * 1988-10-04 1999-09-06 ディーエヌエー プラント テクノロジー コーポレイション 検出可能な表現型のファージ形質導入による細菌の検出

Also Published As

Publication number Publication date
EP0495960B1 (en) 1997-03-19
US5723330A (en) 1998-03-03
ATE150489T1 (de) 1997-04-15
JP3088014B2 (ja) 2000-09-18
DE69125263T2 (de) 1997-09-18
WO1992002633A1 (en) 1992-02-20
DK0495960T3 (da) 1997-08-25
GB9017443D0 (en) 1990-09-26
DE69125263D1 (de) 1997-04-24
CA2066561C (en) 2003-10-14
CA2066561A1 (en) 1992-02-10
JPH05501958A (ja) 1993-04-15
EP0495960A1 (en) 1992-07-29
US5498525A (en) 1996-03-12

Similar Documents

Publication Publication Date Title
ES2100955T3 (es) Metodo para deteccion microbiana rapida.
Chantratita et al. Biological relevance of colony morphology and phenotypic switching by Burkholderia pseudomallei
Tagg et al. “Fingerprinting” β-haemolytic streptococci by their production of and sensitivity to bacteriocine-like inhibitors
Watson et al. Characterization of the starvation-survival response of Staphylococcus aureus
NO964187L (no) Fremgangsmåte for fremstilling av mykobakterier
DK1198713T3 (da) Påvisning og identifikation af bakteriestammer
AR000061A1 (es) Medio para detectar dentro de un lapso de 24 horasla presencía o ausencia de enterococos en una mue stra liquida cuya procedencia excluye aquellas tomadas de seres humanos y animales superiores.
DK0876504T3 (da) Fremgangsmåde til detektion af bakterier
ATE149208T1 (de) Bakterieller nachweis durch phagentransduktion von nachweisbaren phenotypen
Meng et al. Surface layer protein from Lactobacillus acidophilus NCFM inhibit intestinal pathogen-induced apoptosis in HT-29 cells
Saxena et al. Development of a rapid and sensitive recombinase polymerase amplification‐lateral flow assay for detection of Burkholderia mallei
Edouard et al. Persistence of DNA in a cured patient and positive culture in cases with low antibody levels bring into question diagnosis of Q fever endocarditis
Malek et al. Isolation of keratinophilic fungi and aerobic actinomycetes from park soils in Gorgan, North of Iran
DK1504088T3 (da) Bakteriofager egnede til behandling og forebyggelse af bakterielle infektioner
Barrow Microbial antagonism by Staphylococcus aureus
Brzin The localization of acetylcholinesterase in axonal membranes of frog nerve fibers.
ES2157569T3 (es) Procedimiento de deteccion y/o cuantificacion de un factor gliotoxico.
ATE343642T1 (de) Ef-tu mrna als marker für lebensfähige bakterien
Faine Catalase activity in pathogenic Leptospira
Bashiruddin et al. Detection of Mycoplasma mycoides subspecies mycoides in tissues from an outbreak of contagious bovine pleuropneumonia by culture, immunohistochemistry and polymerase chain reaction
Norris et al. An electron microscope study of sporulation and protein crystal formation in Bacillus cereus var. alesti
Buchanan Clinical laboratory evaluation of a reverse CAMP test for presumptive identification of Clostridium perfringens
Fiss et al. Use of a siderophore detection medium, ethylene glycol degradation, and beta-galactosidase activity in the early presumptive differentiation of Nocardia, Rhodococcus, Streptomyces, and rapidly growing Mycobacterium species
Tagg et al. Inhibitor production by group-G streptococci of human and of animal origin
CN105181837A (zh) 一种结核分枝杆菌检测方法

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 495960

Country of ref document: ES