ES2101489T3 - Prevencion de la disociacion de aminoacidos n-terminales mediatizada por aminopeptidasas endogenas durante la expresion de genes extraños en bacterias. - Google Patents

Prevencion de la disociacion de aminoacidos n-terminales mediatizada por aminopeptidasas endogenas durante la expresion de genes extraños en bacterias.

Info

Publication number
ES2101489T3
ES2101489T3 ES94901914T ES94901914T ES2101489T3 ES 2101489 T3 ES2101489 T3 ES 2101489T3 ES 94901914 T ES94901914 T ES 94901914T ES 94901914 T ES94901914 T ES 94901914T ES 2101489 T3 ES2101489 T3 ES 2101489T3
Authority
ES
Spain
Prior art keywords
amino acid
pro
terminal amino
mediated
bacteria
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94901914T
Other languages
English (en)
Inventor
Markus Heitzmann
Rao Movva
Paul Ramage
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Original Assignee
Ciba Geigy AG
Novartis AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ciba Geigy AG, Novartis AG filed Critical Ciba Geigy AG
Application granted granted Critical
Publication of ES2101489T3 publication Critical patent/ES2101489T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/5412—IL-6
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Paints Or Removers (AREA)

Abstract

SE FACILITA UN PROCESO PARA LA PREPARACION RECOMBINANTE EN UN PATRON BACTERIANO DE FORMA MADURA DE PEPTIDO O PROTEINA DE MAMIFERO DE FORMULA X-PRO-Z, QUE NO ESTA SOMETIDO A PROCESAMIENTO, POR AMINOPEPTIDASAS BACTERIANAS ENDOGENAS. EN LA FORMULA X-PRO-Z, X ES UN AMINOACIDO N-TERMINAL SIMPLE DISTINTO DE PROLINA, Y Z ES LA SECUENCIA RESTANTE DE RESIDUOS DE AMINOACIDO DE LA PROTEINA O PEPTIDO. EL PROCESO COMPRENDE LA INSERCION EN UNA CELULA DEL PATRON BACTERIANO DE UN ADECUADO VECTOR QUE CONTIENE CODIFICADOR DE DNA PARA MET-Y-X-PRO-Z, DONDE Y ES UN AMINOACIDO NATURAL QUE SE PUEDE ADHERIR IN VITRO DESDE X-PRO-Z POR UNA AMINOPEPTIDASA Y QUE IMPARTE RESISTENCIA AL PROCESAMIENTO IN VIVO POR AMINOPEPTIDASAS BACTERIANAS ENDOGENAS. LA CELULA ES INDUCIDA A EXPRESAR EL PRODUCTO MET-Y-X-PRO-Z QUE ENTONCES SE TRATA CON UNA AMINOPEPTIDASA ADECUADA PARA SEPARAR MET E Y.
ES94901914T 1992-11-30 1993-11-29 Prevencion de la disociacion de aminoacidos n-terminales mediatizada por aminopeptidasas endogenas durante la expresion de genes extraños en bacterias. Expired - Lifetime ES2101489T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB929225021A GB9225021D0 (en) 1992-11-30 1992-11-30 Organic compounds

Publications (1)

Publication Number Publication Date
ES2101489T3 true ES2101489T3 (es) 1997-07-01

Family

ID=10725879

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94901914T Expired - Lifetime ES2101489T3 (es) 1992-11-30 1993-11-29 Prevencion de la disociacion de aminoacidos n-terminales mediatizada por aminopeptidasas endogenas durante la expresion de genes extraños en bacterias.

Country Status (11)

Country Link
US (1) US6165746A (es)
EP (1) EP0670907B1 (es)
JP (1) JPH08503376A (es)
AT (1) ATE152772T1 (es)
CA (1) CA2145384A1 (es)
DE (1) DE69310540T2 (es)
DK (1) DK0670907T3 (es)
ES (1) ES2101489T3 (es)
GB (1) GB9225021D0 (es)
GR (1) GR3023348T3 (es)
WO (1) WO1994012659A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6743600B1 (en) 1999-04-30 2004-06-01 Monsanto Technologies Llc Method of removing N-terminal alanine residues from polypeptides with Aeromonas aminopeptidase
US6794159B1 (en) * 1999-04-30 2004-09-21 Pharmacia Corporation Method of removing n-terminal alanine residues from polypeptides with aeromonas aminopeptidase
US8143210B2 (en) 2002-02-14 2012-03-27 The Board Of Trustees Of The Leland Stanford Junior University Enzyme treatment of foodstuffs for celiac sprue
AU2003215272B2 (en) 2002-02-14 2008-04-03 The Board Of Trustees Of The Leland Stanford Junior University Enzyme treatment of foodstuffs for celiac sprue
US7776545B2 (en) 2002-11-20 2010-08-17 The Board Of Trustees Of The Leland Stanford Junior University Diagnostic method for Celiac Sprue
US7579313B2 (en) * 2003-11-18 2009-08-25 The Board Of Trustees Of The Leland Stanford Junior University Transglutaminase inhibitors and methods of use thereof
NZ548166A (en) * 2003-12-01 2010-06-25 Meiji Dairies Corp Peptide inhibiting angiontensin converting enzyme
WO2008115411A1 (en) * 2007-03-16 2008-09-25 The Board Of Trustees Of The Leland Stanford Junior University Combination enzyme therapy for digestion of dietary gluten
WO2008115428A2 (en) * 2007-03-16 2008-09-25 The Board Of Trustees Of The Leland Stanford Junior University A scaleable manufacturing process for cysteine endoprotease b, isoform 2
EP3914706A4 (en) * 2019-01-21 2022-12-28 Encodia, Inc. METHODS AND COMPOSITIONS OF ACCELERATION REACTIONS FOR POLYPEPTIDE ANALYSIS AND RELATED USES

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS50112428A (es) * 1974-02-18 1975-09-03
IL60184A (en) * 1979-05-31 1984-05-31 Schering Ag Process for the specific cleavage of protein sequences from proteins
WO1986001229A1 (en) * 1984-08-16 1986-02-27 Bio-Technology General Corp. Method of removing n-terminal amino acid residues from eucaryotic polypetide analogs and polypeptides produced thereby
WO1986007380A1 (fr) * 1985-06-04 1986-12-18 Takeda Chemical Industries, Ltd. Procede de preparation de polypeptides
US4865974A (en) * 1985-09-20 1989-09-12 Cetus Corporation Bacterial methionine N-terminal peptidase
JPS63258969A (ja) * 1986-12-06 1988-10-26 Lion Corp 金属材の表面被覆組成物
JPH02151669A (ja) * 1988-12-02 1990-06-11 Daicel Chem Ind Ltd 塗料組成物

Also Published As

Publication number Publication date
US6165746A (en) 2000-12-26
ATE152772T1 (de) 1997-05-15
WO1994012659A1 (en) 1994-06-09
DE69310540D1 (de) 1997-06-12
DE69310540T2 (de) 1998-01-02
DK0670907T3 (da) 1997-08-25
CA2145384A1 (en) 1994-06-09
EP0670907B1 (en) 1997-05-07
EP0670907A1 (en) 1995-09-13
GR3023348T3 (en) 1997-08-29
JPH08503376A (ja) 1996-04-16
GB9225021D0 (en) 1993-01-20

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