ES2112057T3 - Metodo de deteccion y/o cuantificacion y/o separacion de celulas apoptosicas dentro de o en una muestra. - Google Patents

Metodo de deteccion y/o cuantificacion y/o separacion de celulas apoptosicas dentro de o en una muestra.

Info

Publication number
ES2112057T3
ES2112057T3 ES95913946T ES95913946T ES2112057T3 ES 2112057 T3 ES2112057 T3 ES 2112057T3 ES 95913946 T ES95913946 T ES 95913946T ES 95913946 T ES95913946 T ES 95913946T ES 2112057 T3 ES2112057 T3 ES 2112057T3
Authority
ES
Spain
Prior art keywords
sample
cells
apoptotic cells
presented
optionally
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95913946T
Other languages
English (en)
Inventor
Christiaan Pe Reutelingsperger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nexins Research BV
Original Assignee
Nexins Research BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8216787&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2112057(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Nexins Research BV filed Critical Nexins Research BV
Application granted granted Critical
Publication of ES2112057T3 publication Critical patent/ES2112057T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2510/00—Detection of programmed cell death, i.e. apoptosis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Zoology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

SE PRESENTA UN PROCEDIMIENTO PARA MEDIR LA APOPTOSIS EN FISIOLOGIA Y PATOLOGIA PARA DAR SOPORTE A DIAGNOSTICOS CLINICOS Y OBSERVAR LA EFICACIA DE MEDICAMENTOS Y TERAPIAS. EN PARTICULAR, SE PRESENTA UN METODO PARA DETECTAR Y/O OPCIONALMENTE CUANTIFICAR Y/O OPCIONALMENTE AISLAR LAS CELULAS APOPTOTICAS EN UNA MUESTRA, EL METODO COMPRENDE: A) LA PUESTA EN CONTACTO DE LA MUESTRA CON UN REACTIVO DETECTABLE QUE TENGA UNA ALTA AFINIDAD PARA LA SERINA DE FOSFATIDILO Y B) DETECTAR CUALITATIVA Y/O OPCIONALMENTE CUANTITATIVAMENTE LAS CELULAS QUE HAN REACCIONADO CON EL REACTIVO DETECTABLE QUE TIENE ALTA AFINIDAD PARA LA SERINA DE FOSFATIDILO PREFERIBLEMENTE CELULAS INTACTAS, LA DETECCION SE PRODUCE ANTES O DESPUES DE UN PASO OPCIONAL, C) PARA AISLAR LAS CELULAS APOPTOTICAS DE LAS CELULAS NO APOPTOTICAS, EL AISLAMIENTO PUEDE PRODUCIRSE DEBIDO AL HECHO DE QUE LAS CELULAS APOPTOTICAS HAN SIDO PROVISTAS EN EL PASO A) DE UN REACTIVO DETECTABLE QUE TIENE UNA ALTA AFINIDAD PARA LA SERINA DE FOSFATIDILO, EL REAGENTE TAMBIEN ES SELECCIONABLE EN UNA MANERA CONOCIDA "PER SE". UN REACTIVO ADECUADO QUE TIENE UNA ALTA AFINIDAD PARA LA SERINA DE FOSFATIDILO ES UN POLIPEPTIDO O UNA PROTEINA CLASIFICADA COMO ANNEXINA. TAMBIEN SE PRESENTA UN EQUIPO PARA LLEVAR A CABO LOS METODOS.
ES95913946T 1994-04-11 1995-04-11 Metodo de deteccion y/o cuantificacion y/o separacion de celulas apoptosicas dentro de o en una muestra. Expired - Lifetime ES2112057T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP94200968 1994-04-11

Publications (1)

Publication Number Publication Date
ES2112057T3 true ES2112057T3 (es) 1998-03-16

Family

ID=8216787

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95913946T Expired - Lifetime ES2112057T3 (es) 1994-04-11 1995-04-11 Metodo de deteccion y/o cuantificacion y/o separacion de celulas apoptosicas dentro de o en una muestra.

Country Status (12)

Country Link
US (1) US5834196A (es)
EP (1) EP0755516B1 (es)
JP (1) JP2824155B2 (es)
AT (1) ATE160875T1 (es)
AU (1) AU689248B2 (es)
CA (1) CA2185535C (es)
DE (1) DE69501161T2 (es)
DK (1) DK0755516T3 (es)
ES (1) ES2112057T3 (es)
NO (1) NO303513B1 (es)
NZ (1) NZ283171A (es)
WO (1) WO1995027903A1 (es)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6558916B2 (en) 1996-08-02 2003-05-06 Axiom Biotechnologies, Inc. Cell flow apparatus and method for real-time measurements of patient cellular responses
US5804436A (en) 1996-08-02 1998-09-08 Axiom Biotechnologies, Inc. Apparatus and method for real-time measurement of cellular response
US6280967B1 (en) * 1996-08-02 2001-08-28 Axiom Biotechnologies, Inc. Cell flow apparatus and method for real-time of cellular responses
CN1265572A (zh) 1997-04-30 2000-09-06 莱兰斯坦福初级大学评议会 活体内造影细胞死亡的方法
US6270980B1 (en) 1997-06-05 2001-08-07 Idun Pharmaceuticals, Inc. Rapid methods for identifying modifiers of cellular apoptosis activity
US6511829B1 (en) * 1997-10-09 2003-01-28 The Regents Of The University Of California GFP-annexin fusion proteins
EP0928968A1 (en) * 1998-01-12 1999-07-14 Universität Basel Screening method for apoptosis and necrosis
IL123429A0 (en) 1998-02-24 1998-09-24 Nst Neurosurvival Technologies Peptides and pharmaceutical compositions comprising same
EP1520588B1 (en) 1998-07-13 2014-12-24 Board Of Regents, The University Of Texas System Uses of antibodies to aminophospholipids for cancer treatment
BR9912053A (pt) 1998-07-13 2001-04-03 Univ Texas Processos de tratamento de câncer utilizando conjugados terapêuticos que se ligam a aminofosfolipìdios
US6818213B1 (en) 1998-07-13 2004-11-16 Board Of Regents, The University Of Texas System Cancer treatment compositions comprising therapeutic conjugates that bind to aminophospholipids
US6406693B1 (en) 1998-07-13 2002-06-18 Board Of Regents, The University Of Texas System Cancer treatment methods using antibodies to aminophospholipids
EP1096955B9 (en) 1998-07-13 2006-07-05 Board of Regents, The University of Texas System Uses of antibodies to aminophospholipids for cancer treatment
IL131266A0 (en) 1999-08-05 2001-01-28 N S T Neurosurvival Technologi Peptides and pharmaceutical compositions comprising same
WO2000010673A1 (en) * 1998-08-24 2000-03-02 Nst Neurosurvival Technologies Ltd. Apparatus and method for capturing particles with surface exposure of anionic phospholipids from biological fluids
IL125908A (en) 1998-08-24 2005-05-17 Nst Neurosurvival Technologies Peptides and pharmaceutical compositions comprising same
US6063580A (en) * 1998-08-31 2000-05-16 Wayne State University Methods for the temporal analysis of programmed cell death in living cells
US5939267A (en) * 1998-08-31 1999-08-17 Wayne State University Methods for the temporal analysis of programmed cell death in living cells
EP1283904A2 (en) * 1999-11-03 2003-02-19 Oncotech, Inc. Cancer diagnosis using cell-sorting and gene-expression-profiles
EP1118334A1 (en) * 2000-01-11 2001-07-25 Aventis Behring Gesellschaft mit beschränkter Haftung Method for the production of conjugates and uses thereof for the prevention and treatment of allergic reactions and autoimmune diseases
FR2809181B1 (fr) * 2000-05-16 2002-10-25 Biocytex Monoreactif pour le dosage des microparticules plaquettaires
EP1223429A1 (de) * 2001-01-13 2002-07-17 Dentognostics GmbH Verfahren zum Nachweis von lebenden Mikroorganismen
US20050013778A1 (en) * 2001-04-03 2005-01-20 Theseus Imaging Corporation Methods and compositions for predicting the response to a therapeutic regimen in a subject having a disease associated with cell death
CA2443314C (en) 2001-04-03 2006-03-21 The Board Of Trustees Of The Leland Stanford Junior University Method of imaging cell death in vivo
US6843980B2 (en) 2001-04-03 2005-01-18 Theseus Imaging, Corp. Methods for using annexin for detecting cell death in vivo and treating associated conditions
EP1472238B1 (en) * 2002-02-01 2012-07-04 Promega Corporation Bioluminescent protease assay
WO2003076589A2 (en) * 2002-03-08 2003-09-18 Kopreski Michael S Analysis of apoptotic bodies in bodily fluids
US7179616B1 (en) 2002-10-07 2007-02-20 University Of Notre Dame Du Lac Method for detecting phosphatidylserine on the surface of cells
AU2004211582A1 (en) * 2003-01-30 2004-08-26 The General Hospital Corporation Compositions for modulating immune cell activity and methods for detection thereof
US20040203069A1 (en) * 2003-04-10 2004-10-14 Chris Reutelingsperger Method for determining and/or isolating lipid-binding compounds
WO2005014801A1 (en) * 2003-08-04 2005-02-17 University Court Of The University Of Edinburgh Improvements in cell culture productivity
US7553632B2 (en) 2004-01-22 2009-06-30 Promega Corporation Luminogenic and nonluminogenic multiplex assay
US7416854B2 (en) * 2004-01-22 2008-08-26 Promega Corporation Luminogenic and nonluminogenic multiplex assay
US7511016B2 (en) * 2004-07-07 2009-03-31 Mosamedix B.V. Annexins, derivatives thereof, and annexin-cys variants, as well as therapeutic and diagnostic uses thereof
EP1771572B1 (en) * 2004-07-14 2008-10-15 Janssen Pharmaceutica N.V. P75ntr screening assay for identifying modulators of apoptosis
GB0425324D0 (en) * 2004-11-17 2004-12-22 Univ Edinburgh Assay method
US7687243B1 (en) 2005-06-06 2010-03-30 Crook Tonia M Automated method for detecting apoptosis in cells
JP2009506772A (ja) * 2005-09-01 2009-02-19 プロメガ コーポレイション 細胞ベースの発光及び非発光プロテアソームアッセイ
UA94920C2 (ru) * 2005-10-31 2011-06-25 Інститут Біології Клітини Нан України Способ быстрого выявления, выделения и подсчета апоптических клеток, который базируется на агглютинации
KR100987547B1 (ko) 2006-06-01 2010-10-12 가톨릭대학교 산학협력단 냉동보관된 제대혈 줄기세포로부터 초기 세포사를 측정하는조성물, 키트 및 그 방법
US20070293893A1 (en) * 2006-06-14 2007-12-20 Craig Stolen Method and apparatus for preconditioning of cells
AU2008239654A1 (en) 2007-04-13 2008-10-23 Promega Corporation Luminescent live and dead cell assay
WO2010043045A1 (en) 2008-10-17 2010-04-22 London Health Sciences Centre Research Inc. Annexin and its use to treat inflammatory disorders
EP2748337B1 (en) 2011-09-23 2019-08-07 Siemens Healthcare Diagnostics Inc. Cell response assay for cancer and methods of producing and using same
WO2019129646A1 (en) 2017-12-29 2019-07-04 F. Hoffmann-La Roche Ag Tissue sample preparation system
CN116773805B (zh) * 2022-03-10 2026-08-11 南方科技大学 一种试剂盒、以及细胞损伤程度的检测方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE59010815D1 (de) * 1989-12-27 1998-04-23 Boehringer Ingelheim Int Verwendung eines antikoagulant als diagnostikum
US5627036A (en) * 1989-12-27 1997-05-06 Boehringer Ingelheim International Gmbh Use of an anticoagulant as a diagnostic agent

Also Published As

Publication number Publication date
NO964308D0 (no) 1996-10-10
JPH09505894A (ja) 1997-06-10
AU689248B2 (en) 1998-03-26
ATE160875T1 (de) 1997-12-15
NZ283171A (en) 1998-02-26
EP0755516B1 (en) 1997-12-03
NO964308L (no) 1996-12-11
WO1995027903A1 (en) 1995-10-19
JP2824155B2 (ja) 1998-11-11
DK0755516T3 (da) 1998-08-10
DE69501161D1 (de) 1998-01-15
NO303513B1 (no) 1998-07-20
US5834196A (en) 1998-11-10
AU2114195A (en) 1995-10-30
DE69501161T2 (de) 1998-03-19
CA2185535A1 (en) 1995-10-19
CA2185535C (en) 2000-10-24
EP0755516A1 (en) 1997-01-29

Similar Documents

Publication Publication Date Title
ATE160875T1 (de) Verfahren zum nachweis und/oder zur bestimmung und/oder zur isolierung von apoptotischen zellen in oder aus einer probe
ITMI942083A0 (it) Strumento diagnostico automatizzato in particolare per l'analisi di campioni in pacchi di prova per un analita
ES2123509T3 (es) Unidad de ensayo de especimenes.
US3819505A (en) Testing apparatus
DE3689856D1 (de) Analyseverfahren und vorrichtung für biologische specimen.
DE60322729D1 (de) Zugängliche assay-einrichtung und verwendungsverfahren
ATE500507T1 (de) Verfahren zum erfassen und analyse von auf lichtbeugung beruhenden messergebnissen
BG104275A (en) Method and device for express analysis of tested parameters in biological samples
Bergamaschini et al. Activation of the contact system in cerebrospinal fluid of patients with Alzheimer disease
BR9711869B1 (pt) mÉtodo e kit para coletar amostras de espÉcimes lÍquidas para teste analÍtico.
ATE401342T1 (de) Verfahren und materialien zum nachweis und zur messung von durch freie radikale verursachten schäden
DE69019795D1 (de) Verfahren zur Feststellung der Empfindlichkeit von HIV-1 für Zidovudine und dazu geeignete Oligonukleotide.
DE69029556D1 (de) Trockenes analytisches Element zur quantitativen Analyse von in Vollblut enthaltenen Analyten
ES2161228T3 (es) Procedimiento para controlar la contaminacion virica en muestras de sangre.
ES2095858T3 (es) Sustratos de beta-galactosidasa.
ES521810A0 (es) Un metodo para identificar y numerar celulas microbianas en una muestra de material desconocido.
ATE404866T1 (de) System und verfahren zur in-vitro-analyse von therapeutischen wirkstoffen
ATE54374T1 (de) Einrichtung und verfahren fuer zytologische und histologische untersuchungen von objekttraegerpraeparaten.
BR9708421A (pt) Processo para a deteccão de doenca provocada por prions teste diagnostico ou kit de teste e uso do processo
ES2119001T3 (es) Determinacion de un analito en una muestra liquida.
EP0724619A4 (en) CLEANING SOLUTION FOR ANALYZER
ES2161011T3 (es) Reactivos para ensayo de ion diazonio y metodos para su utilizacion.
ES8604693A1 (es) Un metodo para detectar un anticuerpo o un antigeno contenido en una muestra de fluido de origen biologico.
BR0111975A (pt) Fator, composição, veìculo, ligando, kits de diagnósticos, processos para detectar uma prpsc em uma amostra, e para remover prpsc de material biológico, método para diagnosticar encefalopatias espongiformes humanas transmissìveis e encefalopatias de prìons de animais, e, uso de um fator
EA200201041A1 (ru) Способ прогнозирования преэклампсии и других заболеваний

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 755516

Country of ref document: ES