ES2116342T3 - Cebador para sintesis enzimaticas de acidos nucleicos dependientes de la matriz. - Google Patents

Cebador para sintesis enzimaticas de acidos nucleicos dependientes de la matriz.

Info

Publication number
ES2116342T3
ES2116342T3 ES92917597T ES92917597T ES2116342T3 ES 2116342 T3 ES2116342 T3 ES 2116342T3 ES 92917597 T ES92917597 T ES 92917597T ES 92917597 T ES92917597 T ES 92917597T ES 2116342 T3 ES2116342 T3 ES 2116342T3
Authority
ES
Spain
Prior art keywords
pct
primer
matrix
nucleic acids
sec
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92917597T
Other languages
English (en)
Inventor
Hartmut Seliger
Wojciech T Markiewicz
Gabriele Groger
Rudi Rosch
Margit Klotz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2116342T3 publication Critical patent/ES2116342T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Engineering & Computer Science (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

SE DESCRIBE LA SINTESIS Y LA UTILIZACION DE SOPORTES POLIMEROS CARGADOS CON BASES DE ACIDO NUCLEICO, QUE CONTIENEN POR SU PARTE GRUPOS DE MARCADO O SUS ETAPAS PRECURSORAS. LOS SOPORTES POLIMEROS ASI CARGADOS PUEDEN SER UTILIZADOS COMO UNA FASE LIQUIDA O SOLIDA PARA EL MONTAJE DE OLIGONUCLEOTIDOS UTILIZADOS COMO AGENTE PARA LA SINTESIS DE ACIDOS NUCLEICOS ENZIMATICOS DEPENDIENTES DE MATRICES, POR EJEMPLO PARA EL ANALISIS SECUENCIAL DE ACUERDO CON SANGER Y COLABORADORES O PARA LA REACCION (PCR) EN CADENA POLIMERASA.
ES92917597T 1991-08-28 1992-08-22 Cebador para sintesis enzimaticas de acidos nucleicos dependientes de la matriz. Expired - Lifetime ES2116342T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4128480 1991-08-28

Publications (1)

Publication Number Publication Date
ES2116342T3 true ES2116342T3 (es) 1998-07-16

Family

ID=6439283

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92917597T Expired - Lifetime ES2116342T3 (es) 1991-08-28 1992-08-22 Cebador para sintesis enzimaticas de acidos nucleicos dependientes de la matriz.

Country Status (10)

Country Link
US (1) US5574141A (es)
EP (1) EP0600965B1 (es)
JP (1) JP2562862B2 (es)
KR (1) KR0123559B1 (es)
AT (1) ATE163308T1 (es)
CA (1) CA2113478C (es)
DE (2) DE59209203D1 (es)
DK (1) DK0600965T3 (es)
ES (1) ES2116342T3 (es)
WO (1) WO1993005060A1 (es)

Families Citing this family (21)

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Publication number Priority date Publication date Assignee Title
DE4418691A1 (de) * 1994-05-28 1996-02-22 Boehringer Mannheim Gmbh 3'-(4'-) nicht-radioaktiv markierte Nukleoside und Nukleotide mit Aminocarbonsäure-, Peptid- oder Carbonsäure-Spacer
CA2279673A1 (en) * 1995-12-15 1997-06-16 Enzo Therapeutics, Inc. Property effecting and/or property exhibiting constructs for the expression of non-native nucleic acids for therapeutic and diagnostic uses
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
US6235483B1 (en) * 2000-01-31 2001-05-22 Agilent Technologies, Inc. Methods and kits for indirect labeling of nucleic acids
EP1423400B2 (en) * 2001-03-19 2013-05-22 President and Fellows of Harvard College Evolving new molecular function
IL159268A0 (en) 2001-06-20 2004-06-01 Nuevolution As Templated molecules and methods for using such molecules
WO2004016767A2 (en) * 2002-08-19 2004-02-26 The President And Fellows Of Harvard College Evolving new molecular function
US8017323B2 (en) * 2003-03-26 2011-09-13 President And Fellows Of Harvard College Free reactant use in nucleic acid-templated synthesis
WO2007011722A2 (en) 2005-07-15 2007-01-25 President And Fellows Of Harvard College Reaction discovery system
EP2478013B1 (en) 2009-09-16 2018-10-24 F.Hoffmann-La Roche Ag Coiled coil and/or tether containing protein complexes and uses thereof
EP2519633B1 (en) 2009-12-29 2017-10-25 CuRNA, Inc. Treatment of nuclear respiratory factor 1 (nrf1) related diseases by inhibition of natural antisense transcript to nrf1
TW201138821A (en) 2010-03-26 2011-11-16 Roche Glycart Ag Bispecific antibodies
EP2655414B1 (en) 2010-12-23 2018-08-29 Roche Diagniostics GmbH Bispecific binding agent
WO2012085064A1 (en) 2010-12-23 2012-06-28 Roche Diagnostics Gmbh Detection of a posttranslationally modified polypeptide by a bi-valent binding agent
SG191153A1 (en) 2010-12-23 2013-07-31 Hoffmann La Roche Polypeptide-polynucleotide-complex and its use in targeted effector moiety delivery
CA2861124A1 (en) 2012-02-10 2013-08-15 Genentech, Inc. Single-chain antibodies and other heteromultimers
RU2639287C2 (ru) 2012-06-27 2017-12-20 Ф. Хоффманн-Ля Рош Аг Способ отбора и получения высокоселективных и мультиспецифичных нацеливающих групп с заданными свойствами, включающих по меньшей мере две различные связывающие группировки, и их применения
KR20150030744A (ko) 2012-06-27 2015-03-20 에프. 호프만-라 로슈 아게 표적에 특이적으로 결합하는 하나 이상의 결합 단위를 포함하는 항체 Ｆｃ－영역 접합체의 제조 방법 및 그의 용도
WO2014021938A1 (en) * 2012-08-02 2014-02-06 The Board Of Trustees Of The Leland Stanford Junior University Methods and apparatus for nucleic acid synthesis using oligo-templated polymerization
GB201408623D0 (en) 2014-05-15 2014-07-02 Santaris Pharma As Oligomers and oligomer conjugates
EP3227332B1 (en) 2014-12-03 2019-11-06 F.Hoffmann-La Roche Ag Multispecific antibodies

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU666183A1 (ru) * 1977-06-15 1979-06-05 Новосибирский институт органической химии СО АН СССР Способ получени активных производных динуклеотидов
FR2519005B1 (fr) * 1981-12-29 1985-10-25 Pasteur Institut Fragments d'adn marques a l'une au moins de leurs extremites par des ribonucleotides modifies reconnaissables par des molecules affines et procede pour realiser une analyse de tels fragments d'adn
ES8706823A1 (es) * 1985-03-28 1987-06-16 Cetus Corp Un procedimiento para detectar la presencia o ausencia de al menos una secuencia especifica de acidos nucleicos en una muestra
US4739044A (en) * 1985-06-13 1988-04-19 Amgen Method for derivitization of polynucleotides
US4762779A (en) * 1985-06-13 1988-08-09 Amgen Inc. Compositions and methods for functionalizing nucleic acids
SU1318600A1 (ru) * 1985-10-09 1987-06-23 Всесоюзный Научно-Исследовательский Институт Молекулярной Биологии Способ твердофазного синтеза олигонуклеотидов
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
SU1439108A1 (ru) * 1986-05-11 1988-11-23 Всесоюзный Научно-Исследовательский Институт Молекулярной Биологии Способ получени носителей дл твердофазного синтеза олигонуклеотидов
DE3801987A1 (de) * 1988-01-23 1989-07-27 Boehringer Mannheim Gmbh Traeger zur chemischen oder/und enzymatischen umsetzung von nukleinsaeuren oder nukleinsaeurefragmenten an festphasen
US5082934A (en) * 1989-04-05 1992-01-21 Naxcor Coumarin derivatives for use as nucleotide crosslinking reagents
US5141813A (en) * 1989-08-28 1992-08-25 Clontech Laboratories, Inc. Multifunctional controlled pore glass reagent for solid phase oligonucleotide synthesis
US5403709A (en) * 1992-10-06 1995-04-04 Hybridon, Inc. Method for sequencing synthetic oligonucleotides containing non-phosphodiester internucleotide linkages

Also Published As

Publication number Publication date
DK0600965T3 (da) 1998-09-23
ATE163308T1 (de) 1998-03-15
EP0600965B1 (de) 1998-02-18
CA2113478A1 (en) 1993-03-18
EP0600965A1 (de) 1994-06-15
JPH06506700A (ja) 1994-07-28
CA2113478C (en) 2001-06-12
DE4227882A1 (de) 1993-04-01
DE59209203D1 (de) 1998-03-26
JP2562862B2 (ja) 1996-12-11
WO1993005060A1 (de) 1993-03-18
US5574141A (en) 1996-11-12
KR0123559B1 (ko) 1997-11-28

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