ES2117203T5 - Procedimiento de produccion de enzima dextranasa y decodificacion de su adn. - Google Patents

Procedimiento de produccion de enzima dextranasa y decodificacion de su adn.

Info

Publication number
ES2117203T5
ES2117203T5 ES94203614T ES94203614T ES2117203T5 ES 2117203 T5 ES2117203 T5 ES 2117203T5 ES 94203614 T ES94203614 T ES 94203614T ES 94203614 T ES94203614 T ES 94203614T ES 2117203 T5 ES2117203 T5 ES 2117203T5
Authority
ES
Spain
Prior art keywords
dextranase
decodification
dna
procedure
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94203614T
Other languages
English (en)
Other versions
ES2117203T3 (es
Inventor
Campana Hernan Roca
Garcia Bianca Maria Garcia
Clark Emilio Margollez
Curbelo Dania Mateu
Boada Julio Marcos Delgado
Martinez Luis S Herrera
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centro de Ingenieria Genetica y Biotecnologia CIGB
Original Assignee
Centro de Ingenieria Genetica y Biotecnologia CIGB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=5459391&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2117203(T5) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Centro de Ingenieria Genetica y Biotecnologia CIGB filed Critical Centro de Ingenieria Genetica y Biotecnologia CIGB
Publication of ES2117203T3 publication Critical patent/ES2117203T3/es
Application granted granted Critical
Publication of ES2117203T5 publication Critical patent/ES2117203T5/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2454—Dextranase (3.2.1.11)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Peptides Or Proteins (AREA)

Abstract

UN METODO PARA EL AISLAMIENTO Y LA EXPRESION DE UN GEN QUE CODIFICA LA DEXTRANASA DEL HONGO PENICILLIUM MINIOLUTEUM. ESTA ENZIMA DE ORIGEN FUNGICO SE PRODUCE POR EXPRESION EN ALTOS NIVELES DE LEVADURA. PARA ESTE PROPOSITO, SE AISLA Y SE SECUENCIA UNA COPIA DE ADNC DEL ARNM QUE CODIFICA LA ENZIMA DE DEXTRANASA DEL HONGO PENICILLIUM MINIOLUTEUM . ESTE ADNC SE TRANSFIERE A CELULAS DE PICHIA PASTORIS. SE HAN OBTENIDO LEVADURAS RECOMBINANTES CAPACES DE SEGREGAR LA DEXTRANASA AL MEDIO DE CULTIVO. LA ENZIMA DE DEXTRANASA OBTENIDA PUEDE USARSE, POR EJEMPLO, EN LA INDUSTRIA AZUCARERA PARA HIDROLIZAR EL DEXTRANO EN JUGOS DE CAÑA PARA INCREMENTAR LA PRODUCCION DE AZUCAR.
ES94203614T 1993-12-14 1994-12-13 Procedimiento de produccion de enzima dextranasa y decodificacion de su adn. Expired - Lifetime ES2117203T5 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU1993115 1993-12-14

Publications (2)

Publication Number Publication Date
ES2117203T3 ES2117203T3 (es) 1998-08-01
ES2117203T5 true ES2117203T5 (es) 2001-08-16

Family

ID=5459391

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94203614T Expired - Lifetime ES2117203T5 (es) 1993-12-14 1994-12-13 Procedimiento de produccion de enzima dextranasa y decodificacion de su adn.

Country Status (10)

Country Link
US (1) US5637491A (es)
EP (1) EP0663443B2 (es)
AT (1) ATE164627T1 (es)
AU (1) AU685181B2 (es)
BR (1) BR9404998A (es)
CO (1) CO4440441A1 (es)
DE (1) DE69409354T3 (es)
DK (1) DK0663443T4 (es)
ES (1) ES2117203T5 (es)
ZA (1) ZA949799B (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU685181B2 (en) * 1993-12-14 1998-01-15 Centro De Ingenieria Genetica Y Biotecnologia Dextranase enzyme, method for its production and DNA encoding the enzyme
AU716989B2 (en) * 1996-04-16 2000-03-16 Novozymes A/S Compositions for the removal of dental plaque
AU719536B2 (en) * 1996-06-28 2000-05-11 Novozymes A/S A recombinant enzyme with dextranase activitiy
KR100604401B1 (ko) * 2004-01-30 2006-07-25 주식회사 라이프엔자 아밀로펙틴, 전분, 글리코겐 및 아밀로즈 분해활성을 갖는단백질, 그 단백질을 코딩하는 유전자, 그 발현 세포 및상기 단백질의 생산 방법
KR20050078077A (ko) * 2004-01-30 2005-08-04 주식회사 라이프엔자 뮤탠, 이눌린 및 레반을 분해하는 단백질, 그 단백질을코딩하는 유전자, 그 발현 세포 및 상기 단백질의 생산 방법
KR20070006772A (ko) * 2004-03-11 2007-01-11 가부시키가이샤 시세이도 항노화제 및 콜라겐 생성 촉진제

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU685181B2 (en) * 1993-12-14 1998-01-15 Centro De Ingenieria Genetica Y Biotecnologia Dextranase enzyme, method for its production and DNA encoding the enzyme

Also Published As

Publication number Publication date
ZA949799B (en) 1996-02-05
DE69409354T3 (de) 2001-06-07
EP0663443B1 (en) 1998-04-01
DE69409354D1 (de) 1998-05-07
DK0663443T3 (da) 1998-12-21
CO4440441A1 (es) 1997-05-07
ES2117203T3 (es) 1998-08-01
US5637491A (en) 1997-06-10
AU8027494A (en) 1995-06-22
DK0663443T4 (da) 2001-12-17
BR9404998A (pt) 1995-08-08
DE69409354T2 (de) 1998-07-23
ATE164627T1 (de) 1998-04-15
EP0663443A1 (en) 1995-07-19
EP0663443B2 (en) 2001-03-21
AU685181B2 (en) 1998-01-15

Similar Documents

Publication Publication Date Title
Mizuno et al. A novel protease from yeast with specificity towards paired basic residues
ES2150188T3 (es) Procedimiento para la preparacion de una proteina mediante un hongo transformado por integracion multicopia de un vector de expresion.
Crabos et al. Different translocation of three distinct PKC isoforms with tumor-promoting phorbol ester in human platelets
ATE471984T1 (de) Herstellungsverfahren von glycerin durch rekombinante organismen
KR970707283A (ko) 아스퍼질러스에서 융합 단백질로부터 프로세스된 락토페린과 락토페린 폴리펩티드 단편의 발현 방법(expression of processes recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in aspergillus)
ATE110108T1 (de) Genetische modifizierung von endothelialen zellen.
DE69806586D1 (de) Verbessertes verfahren zur herstellung von mehlteigwaren sowie produkte hergestellt aus solchen teigwaren unter verwendung von glyzerinoxidase
EP4559488A3 (en) Placental tissue grafts and improved methods of preparing and using the same
DK1225227T3 (da) Forbedret forsukring af cellulose ved kloning og amplifikation af beta-glucosidase genet af trichoderma
ES2193130T3 (es) Procedimiento para incrementar la acumulacion de esteroles en plantas.
DK0471754T3 (da) Produktion af vaskulær endotelcelle vækstfaktor, samt DNA kodende for denne
ES2117203T5 (es) Procedimiento de produccion de enzima dextranasa y decodificacion de su adn.
ATE117373T1 (de) Herstellung von guar-alpha-galactosidase mit durch rekombinante dns-verfahren transformierten wirtzellen.
DE69434519D1 (en) Prohormon-konvertase exprimierende polypeptid-synthese
DK1276874T3 (da) Kloning og ekspression af en syreresistent ekstracellulær lipase fra yarrowia lipolytica
ES2091332T3 (es) Procedimiento para hidrolizar hemicelulosa por enzimas producidas por tricoderma reesei.
Sumi et al. Secretory leukocyte protease inhibitor is a novel inhibitor of fibroblast-mediated collagen gel contraction
Huang et al. Investigation on the processing and improving the cleavage efficiency of furin cleavage sites in Pichia pastoris
Volwerk et al. Functional characteristics of phosphatidylinositol-specific phospholipases C from Bacillus cereus and Bacillus thuringiensis
ATE246249T1 (de) Für ammonium-transporter kodierende dna-sequenzen und dieser enthaltende, plasmide, bakterien, hefen und pflanzenzellen
Zarkik et al. Comparative processing of bovine leukemia virus envelope glycoprotein gp72 by subtilisin/kexin‐like mammalian convertases
Kondo et al. Amino acid sequence of phospholipase A from Bungarus multicinctus venom
ATE394419T1 (de) Peptidantagonisten von dp transkriptionsfaktoren
ES2109194B1 (es) Un procedimiento para producir la enzima d-aminoacido oxidasa de rhodotorula gracilis en celulas hospedantes.
CA2146240A1 (en) Increased production of secreted proteins by recombinant eukaryotic cells

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 663443

Country of ref document: ES