ES2117625T3 - Alfa-amilasas microbianas mutantes con estabilidad termica, acida y/o alcalina incrementada. - Google Patents

Alfa-amilasas microbianas mutantes con estabilidad termica, acida y/o alcalina incrementada.

Info

Publication number
ES2117625T3
ES2117625T3 ES90201706T ES90201706T ES2117625T3 ES 2117625 T3 ES2117625 T3 ES 2117625T3 ES 90201706 T ES90201706 T ES 90201706T ES 90201706 T ES90201706 T ES 90201706T ES 2117625 T3 ES2117625 T3 ES 2117625T3
Authority
ES
Spain
Prior art keywords
amylases
alkaline
acid
thermal stability
increased thermal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90201706T
Other languages
English (en)
Inventor
Wilhelmus Johannes Quax
Yves Laroche
Adrianus Wilhelmus Vollebregt
Patrick Stanssens
Marc Lauwereys
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer CropScience NV
Danisco US Inc
Original Assignee
Plant Genetic Systems NV
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Plant Genetic Systems NV, Genencor International Inc filed Critical Plant Genetic Systems NV
Application granted granted Critical
Publication of ES2117625T3 publication Critical patent/ES2117625T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Tires In General (AREA)
  • Cigarettes, Filters, And Manufacturing Of Filters (AREA)

Abstract

SE SUMINISTRAN AMILASAS ABLES COMO PRODUCTOS DE EXPRESION DE GENES DE AMILASA ALFA TRATADOS MEDIANTE INGENIERIA GENETICA AISLADOS A PARTIR DE MICROORGANISMOS, QUE PREFERIBLEMENTE PERTENECEN A LA CLASE DE LOS BACILOS. AMBOS METODOS DE MUTAGENESIS ENZIMATICA Y QUIMICA SON POR EJ. EL METODO DEL DISULFITO Y LA MESOINCORPORACION ENZIMATICA SOBRE EL DNA HETERODUPLEX ESPACIADO. LAS AMILASAS ALFA MUTANTES TIENEN UNA PROPIEDADES SUPERIORES, POR EJ. TERMOESTABILIDAD MEJORADA SOBRE UNA AMPLIA GAMA DE PH PARA APLICACIONES INDUSTRIALES EN EL PROCESAMIENTO DEL ALMIDON Y EN EL DESALMIDONADO TEXTIL.
ES90201706T 1989-06-29 1990-06-27 Alfa-amilasas microbianas mutantes con estabilidad termica, acida y/o alcalina incrementada. Expired - Lifetime ES2117625T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP89201735 1989-06-29
PCT/EP1990/001042 WO1991000353A2 (en) 1989-06-29 1990-06-27 MUTANT MICROBIAL α-AMYLASES WITH INCREASED THERMAL, ACID AND/OR ALKALINE STABILITY

Publications (1)

Publication Number Publication Date
ES2117625T3 true ES2117625T3 (es) 1998-08-16

Family

ID=8202426

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90201706T Expired - Lifetime ES2117625T3 (es) 1989-06-29 1990-06-27 Alfa-amilasas microbianas mutantes con estabilidad termica, acida y/o alcalina incrementada.

Country Status (17)

Country Link
US (1) US5364782A (es)
EP (1) EP0410498B1 (es)
JP (2) JP3086249B2 (es)
KR (1) KR0165550B1 (es)
CN (1) CN1050220A (es)
AT (1) ATE166922T1 (es)
BG (1) BG61081B1 (es)
BR (1) BR9006818A (es)
CA (1) CA2030554C (es)
DD (1) DD301620A9 (es)
DE (1) DE69032360T2 (es)
DK (1) DK0410498T3 (es)
ES (1) ES2117625T3 (es)
FI (1) FI103285B1 (es)
IE (1) IE902369A1 (es)
PT (1) PT94560B (es)
WO (1) WO1991000353A2 (es)

Families Citing this family (144)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1654692A (en) * 1991-05-06 1992-12-21 Valio Finnish Co-Operative Dairies Association Acid alpha-amylase
JPH07503363A (ja) * 1991-11-14 1995-04-13 ノボザイムス アクティーゼルスカブ バシラス・リヘニフォルミス中で遺伝子を発現させる方法
AU5659094A (en) * 1992-12-09 1994-07-04 Yoshiyuki Takasaki Novel acid- and heat-resistant alpha-amylase, process for producing the same, and method of liquefying starch by using the same
DK154292D0 (da) * 1992-12-23 1992-12-23 Novo Nordisk As Nyt enzym
PT867504E (pt) * 1993-02-11 2003-08-29 Genencor Int Alfa-amilase estavel a oxidacao
DK0670367T3 (da) * 1993-05-19 2003-11-24 Kao Corp Flydendegørende alkalisk alfa-amylase, fremgangsmåde til fremstilling af denne og detergentsammensætning indeholdende denne
CA2173329C (en) * 1993-10-08 2011-07-12 Henrik Bisgard-Frantzen Amylase variants
DE69434635D1 (en) * 1993-10-08 2006-04-27 Novo Nordisk As Amylasevarianten
US5830837A (en) * 1994-11-22 1998-11-03 Novo Nordisk A/S Amylase variants
MY111537A (en) * 1994-02-02 2000-07-31 Novo Nordisk As A combined desizing and bleaching process.
US5824531A (en) * 1994-03-29 1998-10-20 Novid Nordisk Alkaline bacilus amylase
TW255887B (en) * 1994-05-25 1995-09-01 Lilly Co Eli Synthesis of benzoquinolinones
DK0772684T3 (da) * 1994-06-17 2005-12-12 Genencor Int Amylolytiske enzymer afledt fra B. Licheniformis alpha-amylasen med forbedrede karakteristika
WO1996017056A1 (en) * 1994-12-02 1996-06-06 Institut Pasteur Hypermutagenesis
KR100511499B1 (ko) * 1995-02-03 2005-12-21 노보자임스 에이/에스 소정 특성을 가지는 알파-아밀라제 돌연변이체를 디자인하는 방법
CN101117629B (zh) * 1995-02-03 2014-06-11 诺维信公司 淀粉酶变体
US6093562A (en) * 1996-02-05 2000-07-25 Novo Nordisk A/S Amylase variants
US6440716B1 (en) 1995-02-03 2002-08-27 Novozymes A/S α-amylase mutants
US7115409B1 (en) 1995-02-03 2006-10-03 Novozymes A/S α-amylase mutants
AR000862A1 (es) 1995-02-03 1997-08-06 Novozymes As Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
KR19980702782A (ko) * 1995-03-09 1998-08-05 혼 마가렛 에이. 녹말 액화 방법
US5736499A (en) * 1995-06-06 1998-04-07 Genencor International, Inc. Mutant A-amylase
JP3025627B2 (ja) 1995-06-14 2000-03-27 花王株式会社 液化型アルカリα−アミラーゼ遺伝子
AR003020A1 (es) * 1995-07-24 1998-05-27 Procter & Gamble Composicion detergente que comprende una amilasa de estabilidad oxidativa aumentada y un sistema surfactante especifico.
WO1997010342A1 (en) * 1995-09-13 1997-03-20 Genencor International, Inc. Alkaliphilic and thermophilic microorganisms and enzymes obtained therefrom
ES2432519T3 (es) * 1996-04-30 2013-12-04 Novozymes A/S Mutantes de alfa-amilasa
US6197070B1 (en) 1996-05-15 2001-03-06 The Procter & Gamble Company Detergent compositions comprising alpha combination of α-amylases for malodor stripping
US5958739A (en) * 1996-06-06 1999-09-28 Genencor International Inc. Mutant α-amylase
US6066779A (en) * 1997-04-28 2000-05-23 Yan's Heterosis & Herbicide, Inc. Crop heterosis and herbicide
US6080568A (en) * 1997-08-19 2000-06-27 Genencor International, Inc. Mutant α-amylase comprising modification at residues corresponding to A210, H405 and/or T412 in Bacillus licheniformis
US6361989B1 (en) 1997-10-13 2002-03-26 Novozymes A/S α-amylase and α-amylase variants
US6187576B1 (en) 1997-10-13 2001-02-13 Novo Nordisk A/S α-amylase mutants
US6204232B1 (en) 1997-10-30 2001-03-20 Novo Nordisk A/S α-amlase mutants
BRPI9911086B1 (pt) 1998-06-10 2016-08-02 Novozymes As composição de limpeza, processo para tratar tecidos a máquina, e uso de uma mananase
US6410295B1 (en) 1999-03-30 2002-06-25 Novozymes A/S Alpha-amylase variants
WO2000060059A2 (en) 1999-03-30 2000-10-12 NovozymesA/S Alpha-amylase variants
EP1214441A1 (en) * 1999-09-01 2002-06-19 Novozymes A/S Maltogenic amylase-modified starch derivatives
JP5571274B2 (ja) 2000-03-08 2014-08-13 ノボザイムス アクティーゼルスカブ 改変された特性を有する変異体
CN100491525C (zh) 2000-07-28 2009-05-27 汉高两合股份公司 从芽孢杆菌a7-7(dsm 12368)中提取的新型淀粉分解酶以及含有该新型淀粉分解酶的洗涤剂和清洗剂
US20020155574A1 (en) * 2000-08-01 2002-10-24 Novozymes A/S Alpha-amylase mutants with altered properties
EP2308980A3 (en) 2000-08-01 2011-04-27 Novozymes A/S Alpha-amylase mutants with altered properties
PL361363A1 (en) 2000-11-28 2004-10-04 Henkel Kommanditgesellschaft Auf Aktien Novel cyclodextrin glucanotransferase (cgtase), obtained from bacillus agaradherens (dsm 9948) and detergents and cleaning agents containing said novel cyclodextrin glucanotransferase
JP3753945B2 (ja) 2001-02-14 2006-03-08 ヒゲタ醤油株式会社 大腸菌とブレビバチルス属細菌間のプラスミドシャトルベクター
ATE449840T1 (de) 2001-05-15 2009-12-15 Novozymes As Alpha-amylasevariante mit veränderten eigenschaften
DE10138753B4 (de) * 2001-08-07 2017-07-20 Henkel Ag & Co. Kgaa Wasch- und Reinigungsmittel mit Hybrid-Alpha-Amylasen
DE10163748A1 (de) 2001-12-21 2003-07-17 Henkel Kgaa Neue Glykosylhydrolasen
US20060042020A1 (en) * 2002-12-20 2006-03-02 Novozymes North America, Inc. Treatment of fabrics, fibers, or yarns
EP2385104A1 (en) * 2003-04-01 2011-11-09 Danisco US Inc. Variant Scytalidium thermophilium CBH1
ES2554635T3 (es) 2004-07-05 2015-12-22 Novozymes A/S Variantes de alfa-amilasa con propiedades alteradas
DE102004047777B4 (de) 2004-10-01 2018-05-09 Basf Se Alpha-Amylase-Varianten mit erhöhter Lösungsmittelstabilität, Verfahren zu deren Herstellung sowie deren Verwendung
CN100396777C (zh) * 2005-10-28 2008-06-25 南开大学 一种嗜热碱性α-淀粉酶及其编码基因
EP1876285A1 (de) * 2006-07-05 2008-01-09 DyStar Textilfarben GmbH & Co. Deutschland KG Kombinierte Entmineralisierung und Entschlichtung von Textilfasermaterialien
KR20100088675A (ko) 2007-11-05 2010-08-10 다니스코 유에스 인크. 변경된 특성을 지닌 바실러스 종 ｔｓ-23 알파-아밀라아제의 변이체
US8206966B2 (en) 2007-11-05 2012-06-26 Danisco Us Inc. Alpha-amylase variants with altered properties
AU2009212526A1 (en) 2008-02-04 2009-08-13 Danisco Us Inc. TS23 alpha-amylase variants with altered properties
MX2010013113A (es) 2008-06-06 2010-12-21 Danisco Inc Variantes de alfa-amilasa geobacillus stearothermophilus con propiedades mejoradas.
MX364987B (es) * 2008-06-06 2019-05-17 Danisco Us Inc Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas.
WO2010036515A1 (en) 2008-09-25 2010-04-01 Danisco Us Inc. Alpha-amylase blends and methods for using said blends
WO2011076897A1 (en) 2009-12-22 2011-06-30 Novozymes A/S Use of amylase variants at low temperature
US20110150955A1 (en) 2009-12-23 2011-06-23 Shannon Elizabeth Klingman Products and Methods for Reducing Malodor from the Pudendum
WO2011080353A1 (en) 2010-01-04 2011-07-07 Novozymes A/S Stabilization of alpha-amylases towards calcium depletion and acidic ph
EP2357220A1 (en) 2010-02-10 2011-08-17 The Procter & Gamble Company Cleaning composition comprising amylase variants with high stability in the presence of a chelating agent
EP3404087A1 (en) 2010-02-10 2018-11-21 Novozymes A/S Alpha-amylase variants with high stability in presence of a chelating agent
US9434932B2 (en) 2011-06-30 2016-09-06 Novozymes A/S Alpha-amylase variants
EP3004342B1 (en) 2013-05-29 2023-01-11 Danisco US Inc. Novel metalloproteases
WO2014194034A2 (en) 2013-05-29 2014-12-04 Danisco Us Inc. Novel metalloproteases
US20160108388A1 (en) 2013-05-29 2016-04-21 Danisco Us Inc. Novel metalloproteases
WO2014194032A1 (en) 2013-05-29 2014-12-04 Danisco Us Inc. Novel metalloproteases
DK3080262T3 (da) 2013-12-13 2019-05-06 Danisco Us Inc Serinproteaser af bacillus-arter
EP3910057A1 (en) 2013-12-13 2021-11-17 Danisco US Inc. Serine proteases of the bacillus gibsonii-clade
KR20160099629A (ko) 2013-12-16 2016-08-22 이 아이 듀폰 디 네모아 앤드 캄파니 점도 조절제로서의 폴리 알파-1,3-글루칸 에테르의 사용
ES2835703T3 (es) 2013-12-18 2021-06-23 Nutrition & Biosciences Usa 4 Inc Eteres de poli alfa-1,3-glucano catiónicos
WO2015123323A1 (en) 2014-02-14 2015-08-20 E. I. Du Pont De Nemours And Company Poly-alpha-1,3-1,6-glucans for viscosity modification
EP3116914B8 (en) 2014-03-11 2021-04-21 E. I. du Pont de Nemours and Company Oxidized poly alpha-1,3-glucan as detergent builder
US20170096653A1 (en) 2014-03-21 2017-04-06 Danisco Us Inc. Serine proteases of bacillus species
US9714403B2 (en) 2014-06-19 2017-07-25 E I Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
EP3158043B1 (en) 2014-06-19 2021-03-10 Nutrition & Biosciences USA 4, Inc. Compositions containing one or more poly alpha-1,3-glucan ether compounds
DK3207129T3 (da) 2014-10-17 2020-02-24 Danisco Us Inc Serinproteaser af bacillus-arten
DK3212781T3 (da) 2014-10-27 2019-12-16 Danisco Us Inc Serinproteaser
DK3212662T3 (da) 2014-10-27 2020-07-20 Danisco Us Inc Serinproteaser
CN107148472A (zh) 2014-10-27 2017-09-08 丹尼斯科美国公司 芽孢杆菌属物种的丝氨酸蛋白酶
EP3212783B1 (en) 2014-10-27 2024-06-26 Danisco US Inc. Serine proteases
EP3957729A1 (en) 2014-10-27 2022-02-23 Danisco US Inc. Serine proteases
CN108064306B (zh) 2014-12-23 2022-11-01 营养与生物科学美国4公司 酶促产生的纤维素
CN107835855B (zh) 2015-05-13 2022-05-13 丹尼斯科美国公司 AprL-进化枝蛋白酶变体及其用途
FI3307427T3 (fi) 2015-06-09 2023-11-09 Danisco Us Inc Osmoottiset puhkeavat kapselit
WO2016201040A1 (en) 2015-06-09 2016-12-15 Danisco Us Inc. Water-triggered enzyme suspension
WO2016201069A1 (en) 2015-06-09 2016-12-15 Danisco Us Inc Low-density enzyme-containing particles
US11499146B2 (en) 2015-06-17 2022-11-15 Danisco Us Inc. Bacillus gibsonii-clade serine proteases
JP7364330B2 (ja) 2015-11-05 2023-10-18 ダニスコ・ユーエス・インク パエニバチルス（Ｐａｅｎｉｂａｃｉｌｌｕｓ）属種及びバチルス（Ｂａｃｉｌｌｕｓ）属種のマンナナーゼ
EP4141113A1 (en) 2015-11-05 2023-03-01 Danisco US Inc Paenibacillus sp. mannanases
EP3374401B1 (en) 2015-11-13 2022-04-06 Nutrition & Biosciences USA 4, Inc. Glucan fiber compositions for use in laundry care and fabric care
JP7045313B2 (ja) 2015-11-13 2022-03-31 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド 洗濯ケアおよび織物ケアにおいて使用するためのグルカン繊維組成物
WO2017083226A1 (en) 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Glucan fiber compositions for use in laundry care and fabric care
US11920170B2 (en) 2015-12-09 2024-03-05 Danisco Us Inc. Alpha-amylase combinatorial variants
EP3390625B1 (en) 2015-12-18 2023-09-06 Danisco US Inc. Polypeptides with endoglucanase activity and uses thereof
CN105802940B (zh) * 2016-04-18 2019-04-16 广西大学 一种地衣芽胞杆菌高温α-淀粉酶突变体及其应用
JP2019518440A (ja) 2016-05-03 2019-07-04 ダニスコ・ユーエス・インク プロテアーゼ変異体およびその使用
CN109072213A (zh) 2016-05-05 2018-12-21 丹尼斯科美国公司 蛋白酶变体及其用途
WO2017210295A1 (en) 2016-05-31 2017-12-07 Danisco Us Inc. Protease variants and uses thereof
EP3472313B1 (en) 2016-06-17 2022-08-31 Danisco US Inc. Protease variants and uses thereof
EP3535365A2 (en) 2016-11-07 2019-09-11 Danisco US Inc. Laundry detergent composition
CN110312795B (zh) 2016-12-21 2024-07-23 丹尼斯科美国公司 蛋白酶变体及其用途
EP3559226B1 (en) 2016-12-21 2023-01-04 Danisco US Inc. Bacillus gibsonii-clade serine proteases
US11453871B2 (en) 2017-03-15 2022-09-27 Danisco Us Inc. Trypsin-like serine proteases and uses thereof
EP3601515A1 (en) 2017-03-31 2020-02-05 Danisco US Inc. Delayed release enzyme formulations for bleach-containing detergents
MX2019014556A (es) 2017-06-30 2020-02-07 Danisco Us Inc Particulas que contienen enzimas de baja aglomeracion.
US20200354708A1 (en) 2017-11-29 2020-11-12 Danisco Us Inc. Subtilisin variants having improved stability
MX2020006518A (es) 2017-12-21 2020-10-28 Danisco Us Inc Gránulos de fusión en caliente, que contienen enzimas, que comprenden un desecante termotolerante.
US20200359656A1 (en) 2018-02-08 2020-11-19 Danisco Us Inc. Thermally-resistant wax matrix particles for enzyme encapsulation
EP3799601A1 (en) 2018-06-19 2021-04-07 Danisco US Inc. Subtilisin variants
WO2019245704A1 (en) 2018-06-19 2019-12-26 Danisco Us Inc Subtilisin variants
WO2020047215A1 (en) 2018-08-30 2020-03-05 Danisco Us Inc Enzyme-containing granules
WO2020068486A1 (en) 2018-09-27 2020-04-02 Danisco Us Inc Compositions for medical instrument cleaning
US12509672B2 (en) 2018-11-28 2025-12-30 Danisco Us Inc. Subtilisin variants having improved stability
US20220220419A1 (en) 2019-05-24 2022-07-14 Danisco Us Inc Subtilisin variants and methods of use
US20220306968A1 (en) 2019-06-06 2022-09-29 Danisco Us Inc Methods and compositions for cleaning
US12157869B2 (en) 2019-07-10 2024-12-03 Jeffrey Dean Lindsay Methods and compositions for reducing persistent odor in clothing and mitigating biofilms on various materials
US20230313209A1 (en) * 2020-08-18 2023-10-05 Novozymes A/S Dispersins expressed with amylase signal peptides
EP4204553A1 (en) 2020-08-27 2023-07-05 Danisco US Inc. Enzymes and enzyme compositions for cleaning
EP4284906A1 (en) 2021-01-29 2023-12-06 Danisco US Inc. Compositions for cleaning and methods related thereto
EP4363565A1 (en) 2021-06-30 2024-05-08 Danisco US Inc. Variant lipases and uses thereof
US20240384205A1 (en) 2021-09-03 2024-11-21 Danisco Us Inc. Laundry compositions for cleaning
CN117957318A (zh) 2021-09-13 2024-04-30 丹尼斯科美国公司 含有生物活性物质的颗粒
CN118679251A (zh) 2021-12-16 2024-09-20 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
WO2023114939A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
EP4448750A2 (en) 2021-12-16 2024-10-23 Danisco US Inc. Subtilisin variants and uses thereof
CN118974227A (zh) 2022-03-01 2024-11-15 丹尼斯科美国公司 用于清洁的酶和酶组合物
EP4525615A2 (en) 2022-05-14 2025-03-26 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN114875057A (zh) * 2022-06-14 2022-08-09 中农华威生物制药(湖北)有限公司 一种可高效表达饲用低温酸性α-淀粉酶的枯草芽孢杆菌的构建方法
WO2023250301A1 (en) 2022-06-21 2023-12-28 Danisco Us Inc. Methods and compositions for cleaning comprising a polypeptide having thermolysin activity
CN120112635A (zh) 2022-09-02 2025-06-06 丹尼斯科美国公司 枯草杆菌蛋白酶变体及其相关方法
WO2024050339A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Mannanase variants and methods of use
EP4581119A1 (en) 2022-09-02 2025-07-09 Danisco US Inc. Detergent compositions and methods related thereto
EP4615968A1 (en) 2022-11-09 2025-09-17 Danisco US Inc. Subtilisin variants and methods of use
CN115806959B (zh) * 2022-12-23 2025-02-18 山东隆科特酶制剂有限公司 碱性淀粉酶突变体及其应用
WO2024163584A1 (en) 2023-02-01 2024-08-08 Danisco Us Inc. Subtilisin variants and methods of use
CN120712348A (zh) 2023-03-06 2025-09-26 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
EP4680013A1 (en) 2023-03-16 2026-01-21 Nutrition & Biosciences USA 4, Inc. Brevibacillus fermentate extracts for cleaning and malodor control and use thereof
CN121909283A (zh) 2023-09-28 2026-04-21 丹尼斯科美国公司 具有改善的溶解度的变体角质酶及其用途
CN117305279B (zh) * 2023-10-08 2024-03-26 态创生物科技(广州)有限公司 高活性和高耐热性的α-淀粉酶突变体及其制备方法和应用
CN121969733A (zh) 2023-10-20 2026-05-01 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
WO2026024921A1 (en) 2024-07-25 2026-01-29 The Procter & Gamble Company Detergent composition comprising a subtilisin variant and methods of use
WO2026050315A1 (en) 2024-08-29 2026-03-05 Danisco Us Inc. Subtilisin variants and methods of use

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4394443A (en) * 1980-12-18 1983-07-19 Yale University Method for cloning genes
CA1170202A (en) * 1981-01-15 1984-07-03 Susan Mickel Process for cloning the gene coding for a thermostable alpha-amylase into escherichia coli and bacillus subtilis
DE3480411D1 (en) * 1983-07-06 1989-12-14 Gist Brocades Nv Molecular cloning and expression in industrial microorganism species
US4740461A (en) * 1983-12-27 1988-04-26 Genetics Institute, Inc. Vectors and methods for transformation of eucaryotic cells
US4717662A (en) * 1985-01-31 1988-01-05 Miles Laboratories, Inc. Thermal stabilization of alpha-amylase
EP0208491B1 (en) * 1985-07-03 1993-08-25 Genencor International, Inc. Hybrid polypeptides and process for their preparation
US5024943A (en) * 1985-11-07 1991-06-18 Gist-Brocades Regulatory region cloning and analysis plasmid for bacillus
DK311186D0 (da) * 1986-06-30 1986-06-30 Novo Industri As Enzymer
NO872932L (no) * 1986-07-18 1988-01-19 Gist Brocades Nv Fremgangsmaate for fremstilling av proteiner med faktorviiiaktivitet ved hjelp av mikrobielle vertsceller, eksprimeringsvektorer, vertsceller, antibiotika.
EP0285123A3 (en) * 1987-04-03 1989-02-01 Stabra AG A method for complete mutagenesis of nucleic acids
WO1989001520A1 (en) * 1987-08-11 1989-02-23 Cetus Corporation Procaryotic xylose isomerase muteins and method to increase protein stability

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US5364782A (en) 1994-11-15
DE69032360T2 (de) 1998-12-03
WO1991000353A2 (en) 1991-01-10
DD301620A9 (de) 1993-04-29
AU638263B2 (en) 1993-06-24
PT94560B (pt) 1998-01-30
FI103285B (fi) 1999-05-31
AU5953890A (en) 1991-01-17
DK0410498T3 (da) 1999-03-22
IE902369A1 (en) 1991-06-19
JP3086249B2 (ja) 2000-09-11
DE69032360D1 (de) 1998-07-09
CA2030554A1 (en) 1990-12-30
PT94560A (pt) 1991-02-08
JP2000197491A (ja) 2000-07-18
CA2030554C (en) 2001-08-28
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EP0410498B1 (en) 1998-06-03
KR0165550B1 (ko) 1999-01-15
IE902369L (en) 1990-12-29
KR920701449A (ko) 1992-08-11
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JPH04500756A (ja) 1992-02-13
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FI103285B1 (fi) 1999-05-31
CN1050220A (zh) 1991-03-27
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