ES2117672T3 - Preparacion de cultivos puros de neuronas humanas post-mitoticas. - Google Patents

Preparacion de cultivos puros de neuronas humanas post-mitoticas.

Info

Publication number
ES2117672T3
ES2117672T3 ES92917219T ES92917219T ES2117672T3 ES 2117672 T3 ES2117672 T3 ES 2117672T3 ES 92917219 T ES92917219 T ES 92917219T ES 92917219 T ES92917219 T ES 92917219T ES 2117672 T3 ES2117672 T3 ES 2117672T3
Authority
ES
Spain
Prior art keywords
cells
post
preparation
pure cultures
human neurons
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92917219T
Other languages
English (en)
Inventor
Virginia Lee
Samuel Pleasure
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Pennsylvania Penn
Original Assignee
University of Pennsylvania Penn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Pennsylvania Penn filed Critical University of Pennsylvania Penn
Application granted granted Critical
Publication of ES2117672T3 publication Critical patent/ES2117672T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C41/00—Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor
    • B29C41/02—Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor for making articles of definite length, i.e. discrete articles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C41/00—Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor
    • B29C41/02—Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor for making articles of definite length, i.e. discrete articles
    • B29C41/12—Spreading-out the material on a substrate, e.g. on the surface of a liquid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C41/00—Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor
    • B29C41/34—Component parts, details or accessories; Auxiliary operations
    • B29C41/36—Feeding the material on to the mould, core or other substrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29L—INDEXING SCHEME ASSOCIATED WITH SUBCLASS B29C, RELATING TO PARTICULAR ARTICLES
    • B29L2023/00—Tubular articles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/30—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from cancer cells, e.g. reversion of tumour cells

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Mechanical Engineering (AREA)
  • Neurology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Neurosurgery (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

CELULAS NTERA 2/CL.DL (NT2), UN TIPO DE CELULA DE TERATOCARCINOMA HUMANA, SE MANIPULARON SIGUIENDO UN TRATAMIENTO DE ACIDO RETINOICO (AR) PARA CONSEGUIR CULTIVOS PUROS EN MAS DEL 95% DE CELULAS NEURONALES (CELULAS NT2-N). ESTE METODO DE CULTIVO ES CAPAZ DE OBTENER CELULAS NT2-N POSTMITOTICAS ALTAMENTE DIFERENCIADAS PARA ESTUDIOS BIOLOGICOS MOLECULARES O BIOQUIMICOS. LAS CELULAS NT2 SE PUEDEN TRANSFERIR EFICIENTEMENTE Y LOS PRODUCTOS DE GEN TRANSFERIDO SE PUEDEN EXPRESAR COMO CELULAS NT2 Y NT2-N.
ES92917219T 1991-10-21 1992-08-05 Preparacion de cultivos puros de neuronas humanas post-mitoticas. Expired - Lifetime ES2117672T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/780,715 US5175103A (en) 1991-10-21 1991-10-21 Preparation of pure cultures of post-mitotic human neurons

Publications (1)

Publication Number Publication Date
ES2117672T3 true ES2117672T3 (es) 1998-08-16

Family

ID=25120453

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92917219T Expired - Lifetime ES2117672T3 (es) 1991-10-21 1992-08-05 Preparacion de cultivos puros de neuronas humanas post-mitoticas.

Country Status (9)

Country Link
US (2) US5175103A (es)
EP (1) EP0620847B1 (es)
JP (1) JP3169611B2 (es)
AT (1) ATE165617T1 (es)
CA (1) CA2120730A1 (es)
DE (1) DE69225332T2 (es)
DK (1) DK0620847T3 (es)
ES (1) ES2117672T3 (es)
WO (1) WO1993008266A1 (es)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5545522A (en) 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
US7049102B1 (en) 1989-09-22 2006-05-23 Board Of Trustees Of Leland Stanford University Multi-gene expression profile
US5750376A (en) * 1991-07-08 1998-05-12 Neurospheres Holdings Ltd. In vitro growth and proliferation of genetically modified multipotent neural stem cells and their progeny
US5980885A (en) * 1991-07-08 1999-11-09 Neurospheres Holdings Ltd. Growth factor-induced proliferation of neural precursor cells in vivo
US7166277B1 (en) 1991-07-08 2007-01-23 Neurospheres Holdings Ltd. Remyelination of neurons using multipotent neural stem cell progeny
US7361505B1 (en) 1991-07-08 2008-04-22 Neurospheres Holdings Ltd. Multipotent neural stem cell compositions
US6497872B1 (en) 1991-07-08 2002-12-24 Neurospheres Holdings Ltd. Neural transplantation using proliferated multipotent neural stem cells and their progeny
US6294346B1 (en) 1991-07-08 2001-09-25 Neurospheres Holdings, Ltd. Use of multipotent neural stem cells and their progeny for the screening of drugs and other biological agents
US6399369B1 (en) 1991-07-08 2002-06-04 Neurospheres Holdings Ltd. Multipotent neural stem cell cDNA libraries
US5851832A (en) * 1991-07-08 1998-12-22 Neurospheres, Ltd. In vitro growth and proliferation of multipotent neural stem cells and their progeny
US6071889A (en) * 1991-07-08 2000-06-06 Neurospheres Holdings Ltd. In vivo genetic modification of growth factor-responsive neural precursor cells
US6214334B1 (en) * 1991-10-21 2001-04-10 Trustees Of The University Of Pennsylvania Compositions and methods for producing and using homogenous neuronal cell transplants to treat neurodegenerative disorders and brain and spinal cord injuries
WO1995012982A1 (en) * 1993-11-09 1995-05-18 The Trustees Of The University Of Pennsylvania Compositions and methods for producing and using homogeneous neuronal cell transplants
US5175103A (en) * 1991-10-21 1992-12-29 Trustees Of University Of Pennsylvania Preparation of pure cultures of post-mitotic human neurons
US5792900A (en) * 1991-10-21 1998-08-11 The Trustees Of The University Of Pennsylvania Compositions and methods for producing and using homogenous neuronal cell transplants
US7011827B2 (en) * 1993-11-09 2006-03-14 Trustees Of The University Of Pennsylvania Compositions and methods for producing and using homogenous neuronal cell transplants
US5449609A (en) * 1994-01-31 1995-09-12 Children's Hospital Of Philadelphia Methods for screening for neurotoxicity using a clonal human teratocarcinoma cell line
US20060029575A1 (en) * 1994-11-09 2006-02-09 Lee Virginia M Compositions and methods for producing and using homogenous neuronal cell transplants
FI971956L (fi) * 1994-11-14 1997-07-04 Neurospheres Holdings Ltd Hermosolujen lisääntymisen säännöstely
US5753505A (en) * 1995-07-06 1998-05-19 Emory University Neuronal progenitor cells and uses thereof
US7544511B2 (en) * 1996-09-25 2009-06-09 Neuralstem Biopharmaceuticals Ltd. Stable neural stem cell line methods
US6162428A (en) * 1997-02-12 2000-12-19 Layton Bioscience, Inc. hNT-neuron human neuronal cells to replace ganglion cells
AU6897498A (en) * 1997-04-25 1998-11-24 American Home Products Corporation Human neuronal cell line
WO1998057663A1 (en) * 1997-06-17 1998-12-23 University Technology Corporation Neural transplantation for treating neurological disease
US6235527B1 (en) * 1997-11-29 2001-05-22 University Of Utah Research Foundation Lineage restricted glial precursors from the central nervous system
JP4371179B2 (ja) * 1997-07-04 2009-11-25 ユニバーシティー オブ ユタ リサーチ ファンデーション 系列限定ニューロン前駆体
US6734015B1 (en) * 1997-07-04 2004-05-11 University Of Utah Research Foundation Isolation of lineage-restricted neuronal precursors
US5968829A (en) 1997-09-05 1999-10-19 Cytotherapeutics, Inc. Human CNS neural stem cells
WO1999013726A1 (en) * 1997-09-19 1999-03-25 University Of South Florida Neuronal cells and an immunosuppressant and anti-inflammatory factor
WO2000006700A1 (en) * 1998-07-29 2000-02-10 Layton Bioscience, Inc. Production and use of dopaminergic cells to treat dopaminergic deficiencies
US20030211085A1 (en) * 2000-03-16 2003-11-13 Sanberg Paul R. Cell therapy for chronic stroke
EP1949904A3 (en) 1999-04-27 2008-08-06 Layton Bioscience, Inc. Cell therapy for chronic stroke
DE19928210B4 (de) * 1999-06-19 2005-08-18 Neuroprogen Gmbh Leipzig Neuronales Zellmaterial und Verfahren zu dessen Herstellung
US6749850B1 (en) 1999-08-18 2004-06-15 The General Hospital Corporation Methods, compositions and kits for promoting recovery from damage to the central nervous system
US7785882B2 (en) * 2000-01-18 2010-08-31 Cornell Research Foundation, Inc. Neuronal progenitor cells from hippocampal tissue and a method for isolating and purifying them
ATE342723T1 (de) 2000-04-13 2006-11-15 Univ Texas Behandlung von neurodegenerativen gastrointestinalen krankheiten durch implantation von neuralen stammzellen und/oder deren nachkommen in gastrointestinalorgane
US7037493B2 (en) 2000-05-01 2006-05-02 Cornell Research Foundation, Inc. Method of inducing neuronal production in the brain and spinal cord
US6897061B1 (en) 2000-06-16 2005-05-24 Spinal Cord Society Transdifferentiation of glial cells
JP4031901B2 (ja) * 2000-07-19 2008-01-09 株式会社東芝 固体撮像装置
ATE420869T1 (de) * 2000-10-05 2009-01-15 Takeda Pharmaceutical Promotoren zur proliferation und differenzierung von stammzellen und/oder vorstufen von neuronenzellen
WO2002063938A2 (en) * 2000-12-05 2002-08-22 Layton Bioscience Inc. Production and use of dopaminergic cells to treat dopaminergic deficiencies
EP1397485A2 (de) * 2001-06-05 2004-03-17 Thomas Rohrmeier Zellpopulationen zur auffindung neuronaler targets und potentieller wirkstoffe
AU2003215297A1 (en) * 2002-02-15 2003-09-09 Cornell Research Foundation, Inc. Enhancing neurotrophin-induced neurogenesis by endogenous neural progenitor cells by concurrent overexpression of brain derived neurotrophic factor and an inhibitor of a pro-gliogenic bone morphogenetic protein
GB0217981D0 (en) * 2002-08-02 2002-09-11 Univ Durham Method for preparation, purification and differentiation of neurospheres from mammalian stem cells
US8293488B2 (en) 2002-12-09 2012-10-23 Neuralstem, Inc. Method for screening neurogenic agents
US20040185429A1 (en) 2002-12-09 2004-09-23 Judith Kelleher-Andersson Method for discovering neurogenic agents
DE10259617A1 (de) * 2002-12-18 2004-07-08 Cardion Ag Verfahren zur Inhibierung des Proliferationspotentials von Zellpopulationen und Geweben
AU2003267969A1 (en) * 2003-04-17 2004-11-26 U.S. Department Of Veterans Affairs Isolated/cloned human nt2 cell lines expressing serotonin and gaba
FR2859219B1 (fr) * 2003-09-02 2005-10-14 Alain Privat Procede de production de neurones a partir de cellules d'une lignee cellulaire
NZ551832A (en) * 2004-06-09 2010-01-29 Univ Edinburgh Neural stem cells
US7663614B2 (en) * 2004-10-05 2010-02-16 Jeff Maynard Method for storing and comparing computer generated lines
EP1814979B1 (en) 2004-11-17 2015-04-08 Neuralstem, Inc. Transplantation of human neural cells for treatment of neurodegenerative conditions
CA2578493A1 (en) * 2007-02-21 2008-08-21 Prince Henry's Institute Of Medical Research A role for sry in parkinson's disease
US20090136461A1 (en) * 2007-11-28 2009-05-28 Moon Suk Kim Neuronal differentiation method of adult stem cells using small molecules
EP3187581A1 (en) 2008-12-23 2017-07-05 BOCO Silicon Valley, Inc. Target populations of oligodendrocyte precursor cells and methods of making and using same
US9101570B1 (en) 2009-02-13 2015-08-11 Endocellutions, Inc. Adult and neonatal stem cell therapy to treat diabetes through the repair of the gastrointestinal tract
NZ623207A (en) 2010-07-28 2014-12-24 Neuralstem Inc Methods for treating and/or reversing neurodegenerative diseases and/or disorders
WO2016064737A1 (en) 2014-10-20 2016-04-28 Neuralstem, Inc. Stable neural stem cells comprising an exogenous polynucleotide coding for a growth factor and methods of use thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4829000A (en) * 1985-08-30 1989-05-09 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Reconstituted basement membrane complex with biological activity
US5032407A (en) * 1987-01-16 1991-07-16 Ohio University Edison Animal Biotechnology Center Gene transfer using transformed, neodetermined, embryonic cells
US5270191A (en) * 1988-04-12 1993-12-14 Massachusetts Institute Of Technology Method for manipulation of the cell types of eukaryotes
FR2645866B1 (fr) * 1989-04-17 1991-07-05 Centre Nat Rech Scient Nouvelles lipopolyamines, leur preparation et leur emploi
US5180820A (en) * 1989-08-30 1993-01-19 Barde Yves Alain Brain-derived neurotrophic factor
US5175103A (en) * 1991-10-21 1992-12-29 Trustees Of University Of Pennsylvania Preparation of pure cultures of post-mitotic human neurons

Also Published As

Publication number Publication date
US5175103A (en) 1992-12-29
JP3169611B2 (ja) 2001-05-28
EP0620847A1 (en) 1994-10-26
DE69225332T2 (de) 1998-08-27
EP0620847B1 (en) 1998-04-29
EP0620847A4 (en) 1995-03-29
DE69225332D1 (de) 1998-06-04
CA2120730A1 (en) 1993-04-29
WO1993008266A1 (en) 1993-04-29
DK0620847T3 (da) 1998-10-07
JPH07502645A (ja) 1995-03-23
ATE165617T1 (de) 1998-05-15
US5654189A (en) 1997-08-05

Similar Documents

Publication Publication Date Title
ES2117672T3 (es) Preparacion de cultivos puros de neuronas humanas post-mitoticas.
Greene Quantitative in vitro studies on the nerve growth factor (NGF) requirement of neurons: I. Sympathetic neurons
Torres-Aleman et al. Trophic effects of insulin-like growth factor-I on fetal rat hypothalamic cells in culture
ES2077034T3 (es) Metodo de cultivo de tejidos para la transformacion de celulas vegetales.
Tamura et al. Normal murine melanocytes in culture
JP2002518990A5 (es)
ATE184797T1 (de) Transplantieren von genetisch modifizierten zellen zur behandlung von krankheiten des zentralen nervensystems
ES2181723T3 (es) Formulaciones farmaceuticas de factor de crecimiento nervioso.
ATE184193T1 (de) Verwendung von n-acylaminoalkoholen zur herstellung eines arzneimittels zur behandlung von mastzellerkrankungen
MXPA02004333A (es) Uso de genomas haploides para diagnostico, modificacion y multiplicacion genetica.
EP0270615A4 (en) TRANSFORMATION AND EXPRESSION OF A FOREIGN GENE IN -i (BRASSICA) SPECIES.
ITMI911662A1 (it) PROCESSO PER LA PURIFICAZIONE DEL VIRUS DELL'EPATITE A (HAV), VIRUS COSIy PURIFICATO E COMPOSIZIONI VACCINALI CHE LO CONTENGONO.
ES2006476A6 (es) Un procedimiento para obtener interleukin-2 humana
SE468815B (sv) Foerfarande foer framstaellning av selenrika alger
CN110257332A (zh) 一种诱导间充质干细胞分化成为多巴胺能神经元的培养基以及方法
Arya et al. Clonal variation of grape‐stem and Phylloxera‐gall callus growing in vitro in different concentrations of sugars
CN105238739A (zh) 临床治疗级细胞治疗用黑色素细胞(melanocytes)规模化制备人类细胞外基质筛选培养方法
Boalch Studies on Ectocarpus in culture. I. Introduction and methods of obtaining uni-algal and bacteria-free cultures
Wood Therapeutic applications: tissue engineering of skin
Bell et al. Interaction of ascorbic acid with the neurotoxic effects of NMDA and sodium nitroprusside
Miller et al. Nutritionally deficient mutants of Marchantia polymorpha induced by X-rays
WO2002063938A3 (en) Production and use of dopaminergic cells to treat dopaminergic deficiencies
US6660305B1 (en) Composition for stimulating the synthesis of the melanic pigment and process for obtaining it
Ojima et al. A karyotypical study of the conger eel (Conger myriaster) in in vitro cells, with special regard to the identification of the sex chromosome
Kaye et al. Cell replication of mesenchymal elements in adult tissues. I. The replication and migration of mesenchymal cells in the adult rabbit dermis

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 620847

Country of ref document: ES