ES2077034T3 - Metodo de cultivo de tejidos para la transformacion de celulas vegetales. - Google Patents

Metodo de cultivo de tejidos para la transformacion de celulas vegetales.

Info

Publication number
ES2077034T3
ES2077034T3 ES90311229T ES90311229T ES2077034T3 ES 2077034 T3 ES2077034 T3 ES 2077034T3 ES 90311229 T ES90311229 T ES 90311229T ES 90311229 T ES90311229 T ES 90311229T ES 2077034 T3 ES2077034 T3 ES 2077034T3
Authority
ES
Spain
Prior art keywords
cells
transformation
cultivation method
vegetable cells
tissue cultivation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90311229T
Other languages
English (en)
Inventor
Dwight Tomes
Dennis Bidney
Charisse Marie Buising
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pioneer Hi-Bred International Inc
Original Assignee
Pioneer Hi-Bred International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi-Bred International Inc filed Critical Pioneer Hi-Bred International Inc
Application granted granted Critical
Publication of ES2077034T3 publication Critical patent/ES2077034T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

UN METODO PARA TRANSFORMAR MAS EFICIENTEMENTE CELULAS DE PLANTAS EN CULTIVO DE TEJIDOS, EL CUAL INCLUYE TRATAMIENTO CON UNA CITOQUININA SEGUIDO POR INCUBACION DURANTE UN PERIODO SUFICIENTE PARA PERMITIR A LAS CELULAS NO DIFERENCIADAS DEL TEJIDO DEL NODO COTILEDONEO DIFERENCIARSE EN CELULAS MERISTEMICAS Y PARA PERMITIR QUE LAS CELULAS ENTREN EN LA FASE DE DESARROLLO ENTRE LA FASE G1 Y LA DE DIVISION.
ES90311229T 1989-10-17 1990-10-12 Metodo de cultivo de tejidos para la transformacion de celulas vegetales. Expired - Lifetime ES2077034T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/422,875 US5322783A (en) 1989-10-17 1989-10-17 Soybean transformation by microparticle bombardment

Publications (1)

Publication Number Publication Date
ES2077034T3 true ES2077034T3 (es) 1995-11-16

Family

ID=23676780

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90311229T Expired - Lifetime ES2077034T3 (es) 1989-10-17 1990-10-12 Metodo de cultivo de tejidos para la transformacion de celulas vegetales.

Country Status (7)

Country Link
US (1) US5322783A (es)
EP (1) EP0424047B1 (es)
AT (1) ATE124995T1 (es)
DE (1) DE69020850T2 (es)
DK (1) DK0424047T3 (es)
ES (1) ES2077034T3 (es)
GR (1) GR3017310T3 (es)

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* Cited by examiner, † Cited by third party
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