ES2127205T3 - Nuevo bioproceso para preparar 7-aca y 7-adac. - Google Patents

Nuevo bioproceso para preparar 7-aca y 7-adac.

Info

Publication number
ES2127205T3
ES2127205T3 ES92309351T ES92309351T ES2127205T3 ES 2127205 T3 ES2127205 T3 ES 2127205T3 ES 92309351 T ES92309351 T ES 92309351T ES 92309351 T ES92309351 T ES 92309351T ES 2127205 T3 ES2127205 T3 ES 2127205T3
Authority
ES
Spain
Prior art keywords
product
bioprocess
converts
prepare
adac
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92309351T
Other languages
English (en)
Inventor
Michael J Conder
John A Rambosek
Anthony Michael Stepan
Phyllis C Mcada
Lorilee Crawford
Christopher D Reeves
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM Delft BV
Original Assignee
Gist Brocades BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gist Brocades BV filed Critical Gist Brocades BV
Application granted granted Critical
Publication of ES2127205T3 publication Critical patent/ES2127205T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
    • C12P37/04—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin by acylation of the substituent in the 6 position
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/933—Penicillium
    • Y10S435/935—Penicillium chrysogenum

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cephalosporin Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Medicinal Preparation (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

INTERMEDIARIOS IMPORTANTES PARA PREPARAR ANTIBIOTICOS DE CEFALOSPORINA, ACIDO 7 CIDO 7 MEDIANTE UN NUEVO BIOPROCESO EN EL QUE UNA CADENA DE PENICILLIUM CHRYSOGENUM TRANSFORMADO SE CULTIVA EN PRESENCIA DE UN MATERIAL DE ALIMENTACION DE ADIPATO PARA PRODUCIR ADIPOIL (ACIDO PENICILANICO 6 ITU DE LOS SIGUIENTES GENES QUE CON LOS QUE SE HA TRANSFORMADO EL P. CHRYSOGENUM: 1) UN GEN DE EXPANDASA, POR EJEMPLO DE CEPHALOSPORIUM ACREMONIUM, CUYO PRODUCTO DE EXPRESION CONVIERTE EL ADIPOIL A; 2) UN GEN DE HIDROXILASA CUYO PRODUCTO DE EXPRESION CONVIERTE LA CADENA LATERAL 3 OXIMETIL, PARA DAR EL PRIMER PRODUCTO, ACIDO 7 AMINODEACETILCEFALOSPORANICO (7 PRODUCTO DE EXPRESION CONVIERTE LA CADENA LATERAL 3 METIL EN CADENA LATERAL 3 UCTO FINAL, 7 NTERA, CONSIGUIENTEMENTE, SE LLEVA A CABO UTILIZANDO BIOPROCESO, Y ES EFICAZ Y ECONOMICA.
ES92309351T 1991-10-15 1992-10-14 Nuevo bioproceso para preparar 7-aca y 7-adac. Expired - Lifetime ES2127205T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US77783391A 1991-10-15 1991-10-15
US95349292A 1992-10-06 1992-10-06

Publications (1)

Publication Number Publication Date
ES2127205T3 true ES2127205T3 (es) 1999-04-16

Family

ID=27119363

Family Applications (2)

Application Number Title Priority Date Filing Date
ES92309351T Expired - Lifetime ES2127205T3 (es) 1991-10-15 1992-10-14 Nuevo bioproceso para preparar 7-aca y 7-adac.
ES97204046T Expired - Lifetime ES2176613T3 (es) 1991-10-15 1992-10-14 Acido adipoil-7-amino-desacetil-cefalosporanico.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES97204046T Expired - Lifetime ES2176613T3 (es) 1991-10-15 1992-10-14 Acido adipoil-7-amino-desacetil-cefalosporanico.

Country Status (27)

Country Link
US (4) US5559005A (es)
EP (2) EP0856516B1 (es)
JP (1) JP2655790B2 (es)
KR (1) KR100227711B1 (es)
CN (1) CN1074484A (es)
AT (2) ATE174057T1 (es)
AU (1) AU657800B2 (es)
BG (1) BG62261B1 (es)
CA (1) CA2080573C (es)
CZ (1) CZ288721B6 (es)
DE (2) DE69227750T2 (es)
DK (2) DK0540210T3 (es)
ES (2) ES2127205T3 (es)
FI (1) FI106965B (es)
GR (1) GR3029343T3 (es)
HU (1) HU219370B (es)
IL (3) IL103419A (es)
MX (1) MX9205902A (es)
NO (1) NO315802B1 (es)
NZ (1) NZ244714A (es)
RO (1) RO116648B1 (es)
RU (1) RU2002130702A (es)
SK (1) SK280569B6 (es)
TW (1) TW272233B (es)
UA (1) UA47385C2 (es)
WO (1) WO1993008287A1 (es)
YU (1) YU48930B (es)

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US6709859B1 (en) 1986-01-31 2004-03-23 Beecham Group P.L.C. Chromosomal DNA Fragments Encoding Enzymes for Encoding B-Lactam Biosynthesis Enzymes, and Vector and Transformants for Their Expression
KR0171897B1 (ko) * 1989-12-27 1999-02-01 후지사와 도모끼찌로 7-아미노세펨 화합물 또는 그 염류의 제조 방법
US5318896A (en) * 1991-09-11 1994-06-07 Merck & Co., Inc. Recombinant expandase bioprocess for preparing 7-aminodesacetoxy cephalosporanic acid (7-ADCA)
BR9407108A (pt) * 1993-07-30 1996-08-27 Gist Brocades Nv Processo para a preparação e recuperação de ácido 7-amino desacetoxi cefalosporânico (7-adca) vetor de ADN recombinante e célula hospedeira transformada com um vetor definido
ES2194873T3 (es) * 1993-07-30 2003-12-01 Dsm Ip Assets Bv Procedimiento para la produccion eficaz de 7-adca a traves de 3-(carboxietil)propionil-7-adca.
RU2139350C1 (ru) * 1993-07-30 1999-10-10 Гист-Брокейдс Б.В. Способ эффективного производства 7-адцк через 3-(карбоксиэтилтио)пропионил-7-адцк
US5882883A (en) * 1994-09-28 1999-03-16 Novo Nordisk A/S Process for the production of secondary metabolites
DE4437420A1 (de) * 1994-10-19 1996-04-25 Hoechst Ag Verbessertes, induktionsfreies Verfahren zur gentechnischen Herstellung von Glutarylamidase
CN1084791C (zh) * 1995-06-02 2002-05-15 吉斯特·布罗卡迪斯股份有限公司 通过扩环酶活性作用于青霉素g生产7－adca的方法
US5731165A (en) * 1995-06-02 1998-03-24 Gist-Brocades, B.V. Process for the production of 7-ADCA via expandase activity on penicillin G
WO1997038107A1 (en) * 1996-04-04 1997-10-16 Gist-Brocades B.V. An enzyme with high adipoyl-coenzyme a synthetase activity and uses thereof
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KR20000022108A (ko) * 1997-04-22 2000-04-25 한스 발터 라벤 탈아실화된 세팔로스포린의 발효제조방법
WO1998048037A1 (en) * 1997-04-22 1998-10-29 Dsm N.V. A METHOD FOR CONTROLLING THE SOLUBILITY OF A β-LACTAM NUCLEUS
US6319684B1 (en) 1997-04-22 2001-11-20 Dms N.V. Process for the fermentative production of cephalosporin
PL330760A1 (en) * 1997-04-22 1999-05-24 Gist Brocades Bv Method of obatining decylated cephalosporins by fermentation process
AU9264598A (en) * 1997-08-22 1999-03-16 Gist-Brocades B.V. Statin production by fermentation
ATE226954T1 (de) 1998-03-27 2002-11-15 Dsm Nv Verfahren zur fermentativen herstellung von cephalosporin
US6383773B2 (en) 1998-04-23 2002-05-07 Massachusetts Institute Of Technology Penicillin conversion
CN100390285C (zh) * 1998-05-19 2008-05-28 Dsm公司 体内生产头孢菌素的改良
CN1331751A (zh) * 1998-12-22 2002-01-16 Dsm公司 改进的头孢菌素的体内生产方法
ES2165276B1 (es) * 1999-06-18 2003-03-01 Antibioticos Sau Procedimiento biotecnologico para la produccion de acido 7-aminocefal osporanico (7-adca) y compuestos de sintesis intermedios particularmente penicilina n y desacetoxicefalosporina c (daoc)
IL152643A0 (en) 2000-05-13 2003-06-24 Smithkline Beecham Plc Process for the purification of a salt of clavulanic acid
US6699699B2 (en) * 2002-03-26 2004-03-02 Synmax Biochemical Co., Ltd. Mutated penicillin expandases
KR20060061295A (ko) * 2003-05-28 2006-06-07 디에스엠 아이피 어셋츠 비.브이. 세펨 화합물
CN101389635B (zh) * 2006-02-23 2013-03-06 中化帝斯曼制药有限公司荷兰公司 改进的头孢菌素生产
KR101569832B1 (ko) * 2008-11-19 2015-11-18 삼성전자주식회사 시료 중의 게놈 ｄｎａ 및 플라스미드 ｄｎａ를 서로 분리하는 방법 및 그를 위한 키트
CN101429538B (zh) * 2008-12-12 2011-05-25 河北九派制药有限公司 7-α-甲氧基-3-去乙酰基头孢噻吩苄星盐的制备方法
CN102369290A (zh) 2009-04-03 2012-03-07 帝斯曼知识产权资产管理有限公司 发酵工艺
US8863444B2 (en) 2010-05-31 2014-10-21 Feature Walters Assembly system for modular building units
WO2011161004A1 (en) 2010-06-22 2011-12-29 Dsm Ip Assets B.V. Air bubble fermentation process
EP2614066B1 (en) 2010-09-07 2017-11-29 DSM Sinochem Pharmaceuticals Netherlands B.V. Process for the production of cephalosporins
CN102702229A (zh) * 2011-08-04 2012-10-03 重庆天地药业有限责任公司 一种头孢唑肟钠关键中间体的制备及高含量头孢唑肟钠的制备
WO2015091455A1 (en) 2013-12-17 2015-06-25 Dpx Holdings B.V. Bioreactor
KR101808192B1 (ko) * 2016-08-26 2018-01-18 아미코젠주식회사 7-아미노세팔로스포란산의 고농도 생산 재조합 아크레모니움 크리소제눔 균주의 제조방법 및 이 방법으로 제조된 균주

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Also Published As

Publication number Publication date
IL133733A0 (en) 2001-04-30
IL103419A (en) 2003-06-24
EP0856516B1 (en) 2002-04-24
FI106965B (fi) 2001-05-15
HU219370B (en) 2001-03-28
NO315802B1 (no) 2003-10-27
HU9401073D0 (en) 1994-07-28
IL103419A0 (en) 1993-03-15
CZ288721B6 (cs) 2001-08-15
YU92592A (sh) 1995-10-03
BG62261B1 (bg) 1999-06-30
KR100227711B1 (ko) 1999-12-01
WO1993008287A1 (en) 1993-04-29
US5629171A (en) 1997-05-13
DE69227750D1 (de) 1999-01-14
TW272233B (es) 1996-03-11
IL133734A0 (en) 2001-04-30
EP0540210B1 (en) 1998-12-02
FI941730L (fi) 1994-04-14
SK280569B6 (sk) 2000-03-13
AU2701692A (en) 1993-04-22
GR3029343T3 (en) 1999-05-28
DE69232581T2 (de) 2002-11-14
US6017726A (en) 2000-01-25
RU2002130702A (ru) 2004-05-20
CN1074484A (zh) 1993-07-21
ATE216700T1 (de) 2002-05-15
CZ88494A3 (en) 1995-03-15
CA2080573A1 (en) 1993-04-16
ES2176613T3 (es) 2002-12-01
YU48930B (sh) 2002-12-10
FI941730A0 (fi) 1994-04-14
NZ244714A (en) 1994-03-25
RO116648B1 (ro) 2001-04-30
EP0856516A1 (en) 1998-08-05
DE69227750T2 (de) 1999-04-22
UA47385C2 (uk) 2002-07-15
ATE174057T1 (de) 1998-12-15
AU657800B2 (en) 1995-03-23
DK0856516T3 (da) 2002-07-29
JP2655790B2 (ja) 1997-09-24
DE69232581D1 (de) 2002-05-29
CA2080573C (en) 2004-01-06
HUT69783A (en) 1995-09-28
JPH06113884A (ja) 1994-04-26
MX9205902A (es) 1994-06-30
US5559005A (en) 1996-09-24
DK0540210T3 (da) 1999-06-23
BG98714A (bg) 1995-02-28
SK43194A3 (en) 1996-11-06
NO941345D0 (no) 1994-04-14
US6071713A (en) 2000-06-06
NO941345L (no) 1994-06-15
EP0540210A1 (en) 1993-05-05

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