ES2176613T3 - Acido adipoil-7-amino-desacetil-cefalosporanico. - Google Patents

Acido adipoil-7-amino-desacetil-cefalosporanico.

Info

Publication number
ES2176613T3
ES2176613T3 ES97204046T ES97204046T ES2176613T3 ES 2176613 T3 ES2176613 T3 ES 2176613T3 ES 97204046 T ES97204046 T ES 97204046T ES 97204046 T ES97204046 T ES 97204046T ES 2176613 T3 ES2176613 T3 ES 2176613T3
Authority
ES
Spain
Prior art keywords
adipoil
acid
aca
gene
side chain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES97204046T
Other languages
English (en)
Inventor
Michael J Conder
Anthony Michael Stepan
Lorilee Crawford
John A Rambosek
Phyllis C Mcada
Christopher D Reeves
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM BV
Original Assignee
DSM NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM NV filed Critical DSM NV
Application granted granted Critical
Publication of ES2176613T3 publication Critical patent/ES2176613T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
    • C12P37/04—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin by acylation of the substituent in the 6 position
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/933—Penicillium
    • Y10S435/935—Penicillium chrysogenum

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cephalosporin Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Medicinal Preparation (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

SE PREPARA EL ACIDO 7 - AMINOCEFALOSPORANICO (7 - ACA) Y EL ACIDO 7 - AMINODESACETILCEFALOSPORANICO (7 - ADAC), IMPORTANTES INTERMEDIOS PARA PREPARAR ANTIBIOTICOS DE CEFALOSPORINA, MEDIANTE UN NUEVO BIOPROCEDIMIENTO EN EL CUAL UNA CEPA TRANSFORMADA DE PENICILLIUM CHRYSOGENUM SE CULTIVA EN PRESENCIA DE UN CULTIVO DE DESARROLLO DE ADIPATO PARA PRODUCIR ADIPOIL - 6 - APA (ACIDO 6 AMINOPENICILANICO); SEGUIDO DE LA EXPRESION IN SITU DE LOS SIGUIENTES GENES CON LOS CUALES HA SIDO TRANSFORMADO EL P. CHRYSOGENUM: 1) UN GEN DE EXPANDASA, POR EJEMPLO PROCEDENTE DE CEPHALOSPORIUM ACREMONIUM, CUYO PRODUCTO DE EXPRESION CONVIERTE EL ADIPOIL - 6 APA MEDIANTE LA EXPANSION DEL ANILLO EN ADIPOIL - 7 - ADCA; 2) UN GEN DE HIDROXILASA CUYO PRODUCTO DE EXPRESION CONVIERTE LA CADENA LATERAL 3 - METILO DEL ADIPOIL 7 - ADCA EN 3 HIDROXIMETILO, PARA PRODUCIR EL PRIMER PRODUCTO, ACIDO ADIPOIL 7 - AMINODESACETILCEFALOSPORANICO (7 - ADAC); Y 3) UN GEN DE ACETILTRANSFERASA CUYO PRODUCTO DE EXPRESION CONVIERTE LA CADENALATERAL 3 - HIDROXIMETILO EN LA CADENA LATERAL 3 - ACETILOXIMETILO DEL ADIPOIL - 7 - ACA. EL PRODUCTO FINAL, 7 - ACA, SE PREPARA ENTONCES MEDIANTE ESCISION DE LA CADENA LATERAL ADIPOILO UTILIZANDO UNA ADIPOILO ACILASA. POR CONSIGUIENTE, LA SINTESIS COMPLETA SE LLEVA A CABO UTILIZANDO BIOPROCEDIMIENTOS, Y RESULTA EFICAZ Y ECONOMICA.
ES97204046T 1991-10-15 1992-10-14 Acido adipoil-7-amino-desacetil-cefalosporanico. Expired - Lifetime ES2176613T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US77783391A 1991-10-15 1991-10-15
US95349292A 1992-10-06 1992-10-06

Publications (1)

Publication Number Publication Date
ES2176613T3 true ES2176613T3 (es) 2002-12-01

Family

ID=27119363

Family Applications (2)

Application Number Title Priority Date Filing Date
ES92309351T Expired - Lifetime ES2127205T3 (es) 1991-10-15 1992-10-14 Nuevo bioproceso para preparar 7-aca y 7-adac.
ES97204046T Expired - Lifetime ES2176613T3 (es) 1991-10-15 1992-10-14 Acido adipoil-7-amino-desacetil-cefalosporanico.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES92309351T Expired - Lifetime ES2127205T3 (es) 1991-10-15 1992-10-14 Nuevo bioproceso para preparar 7-aca y 7-adac.

Country Status (27)

Country Link
US (4) US5559005A (es)
EP (2) EP0856516B1 (es)
JP (1) JP2655790B2 (es)
KR (1) KR100227711B1 (es)
CN (1) CN1074484A (es)
AT (2) ATE174057T1 (es)
AU (1) AU657800B2 (es)
BG (1) BG62261B1 (es)
CA (1) CA2080573C (es)
CZ (1) CZ288721B6 (es)
DE (2) DE69227750T2 (es)
DK (2) DK0540210T3 (es)
ES (2) ES2127205T3 (es)
FI (1) FI106965B (es)
GR (1) GR3029343T3 (es)
HU (1) HU219370B (es)
IL (3) IL103419A (es)
MX (1) MX9205902A (es)
NO (1) NO315802B1 (es)
NZ (1) NZ244714A (es)
RO (1) RO116648B1 (es)
RU (1) RU2002130702A (es)
SK (1) SK280569B6 (es)
TW (1) TW272233B (es)
UA (1) UA47385C2 (es)
WO (1) WO1993008287A1 (es)
YU (1) YU48930B (es)

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EP0233715B1 (en) * 1986-01-31 1994-05-25 BEECHAM GROUP plc Isolation and expression of genes involved in the biosynthesis of beta-lactams
KR0171897B1 (ko) * 1989-12-27 1999-02-01 후지사와 도모끼찌로 7-아미노세펨 화합물 또는 그 염류의 제조 방법
US5318896A (en) * 1991-09-11 1994-06-07 Merck & Co., Inc. Recombinant expandase bioprocess for preparing 7-aminodesacetoxy cephalosporanic acid (7-ADCA)
CN1087780C (zh) * 1993-07-30 2002-07-17 吉斯特·布罗卡迪斯公司 通过3－(羧乙硫基)丙酰－7－adca有效生产7－adca的方法
WO1995004148A1 (en) * 1993-07-30 1995-02-09 Gist-Brocades B.V. Process for the efficient production of 7-adca via 2-(carboxyethylthio)acetyl-7-adca and 3-(carboxymethylthio)propionyl-7-adca
RU2139350C1 (ru) * 1993-07-30 1999-10-10 Гист-Брокейдс Б.В. Способ эффективного производства 7-адцк через 3-(карбоксиэтилтио)пропионил-7-адцк
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DE4437420A1 (de) * 1994-10-19 1996-04-25 Hoechst Ag Verbessertes, induktionsfreies Verfahren zur gentechnischen Herstellung von Glutarylamidase
PL182509B1 (pl) * 1995-06-02 2002-01-31 Gist Brocades Bv Sposób wytwarzania kwasu 7-aminodezacetoksycefalosporanowego (7-ADCA)
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CN1224468A (zh) * 1997-04-22 1999-07-28 吉斯特-布罗卡迪斯有限公司 去酰基化头孢菌素的发酵生产的方法
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ES2165276B1 (es) * 1999-06-18 2003-03-01 Antibioticos Sau Procedimiento biotecnologico para la produccion de acido 7-aminocefal osporanico (7-adca) y compuestos de sintesis intermedios particularmente penicilina n y desacetoxicefalosporina c (daoc)
CA2408853C (en) 2000-05-13 2012-01-10 Smithkline Beecham P.L.C. Process
US6699699B2 (en) * 2002-03-26 2004-03-02 Synmax Biochemical Co., Ltd. Mutated penicillin expandases
CN1795197B (zh) * 2003-05-28 2010-06-02 帝斯曼知识产权资产管理有限公司 头孢菌素类化合物
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KR101569832B1 (ko) * 2008-11-19 2015-11-18 삼성전자주식회사 시료 중의 게놈 ｄｎａ 및 플라스미드 ｄｎａ를 서로 분리하는 방법 및 그를 위한 키트
CN101429538B (zh) * 2008-12-12 2011-05-25 河北九派制药有限公司 7-α-甲氧基-3-去乙酰基头孢噻吩苄星盐的制备方法
CN102369290A (zh) 2009-04-03 2012-03-07 帝斯曼知识产权资产管理有限公司 发酵工艺
US8863444B2 (en) 2010-05-31 2014-10-21 Feature Walters Assembly system for modular building units
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WO2015091455A1 (en) 2013-12-17 2015-06-25 Dpx Holdings B.V. Bioreactor
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Also Published As

Publication number Publication date
IL133734A0 (en) 2001-04-30
NZ244714A (en) 1994-03-25
CA2080573A1 (en) 1993-04-16
WO1993008287A1 (en) 1993-04-29
IL133733A0 (en) 2001-04-30
EP0540210B1 (en) 1998-12-02
EP0856516A1 (en) 1998-08-05
IL103419A (en) 2003-06-24
CN1074484A (zh) 1993-07-21
ES2127205T3 (es) 1999-04-16
US5629171A (en) 1997-05-13
NO941345D0 (no) 1994-04-14
HU9401073D0 (en) 1994-07-28
DK0540210T3 (da) 1999-06-23
EP0540210A1 (en) 1993-05-05
YU92592A (sh) 1995-10-03
HUT69783A (en) 1995-09-28
ATE216700T1 (de) 2002-05-15
EP0856516B1 (en) 2002-04-24
FI106965B (fi) 2001-05-15
HU219370B (en) 2001-03-28
JPH06113884A (ja) 1994-04-26
US6017726A (en) 2000-01-25
DE69227750T2 (de) 1999-04-22
NO941345L (no) 1994-06-15
IL103419A0 (en) 1993-03-15
US5559005A (en) 1996-09-24
FI941730L (fi) 1994-04-14
FI941730A0 (fi) 1994-04-14
CZ88494A3 (en) 1995-03-15
RO116648B1 (ro) 2001-04-30
DK0856516T3 (da) 2002-07-29
UA47385C2 (uk) 2002-07-15
TW272233B (es) 1996-03-11
GR3029343T3 (en) 1999-05-28
SK280569B6 (sk) 2000-03-13
AU657800B2 (en) 1995-03-23
BG62261B1 (bg) 1999-06-30
DE69227750D1 (de) 1999-01-14
KR100227711B1 (ko) 1999-12-01
MX9205902A (es) 1994-06-30
US6071713A (en) 2000-06-06
SK43194A3 (en) 1996-11-06
CA2080573C (en) 2004-01-06
AU2701692A (en) 1993-04-22
RU2002130702A (ru) 2004-05-20
BG98714A (bg) 1995-02-28
CZ288721B6 (cs) 2001-08-15
DE69232581T2 (de) 2002-11-14
DE69232581D1 (de) 2002-05-29
ATE174057T1 (de) 1998-12-15
YU48930B (sh) 2002-12-10
JP2655790B2 (ja) 1997-09-24
NO315802B1 (no) 2003-10-27

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