ES2146646T3 - Sistema de expresion que permite producir una apoliproteina ai-m. - Google Patents

Sistema de expresion que permite producir una apoliproteina ai-m.

Info

Publication number
ES2146646T3
ES2146646T3 ES94902173T ES94902173T ES2146646T3 ES 2146646 T3 ES2146646 T3 ES 2146646T3 ES 94902173 T ES94902173 T ES 94902173T ES 94902173 T ES94902173 T ES 94902173T ES 2146646 T3 ES2146646 T3 ES 2146646T3
Authority
ES
Spain
Prior art keywords
apoliprotein
expression system
production
allows
dna sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94902173T
Other languages
English (en)
Inventor
Lars Abrahmsen
Erik Holmgren
Christina Kaldeden
Mats Lake
Asa Mikaelsson
Torsten Sejlitz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Esperion Therapeutics Inc
Original Assignee
Esperion Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Esperion Therapeutics Inc filed Critical Esperion Therapeutics Inc
Application granted granted Critical
Publication of ES2146646T3 publication Critical patent/ES2146646T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/775—Apolipopeptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Steroid Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Peptides Or Proteins (AREA)

Abstract

EL INVENTO SE REFIERE A UN SISTEMA DE EXPRESION QUE PROPORCIONA UNA ALTA PRODUCCION EXTRACELULAR DE LA APOLIPROTEINA AI-M (MILAN) USANDO E. COLI Y QUE COMPRENDE UNA PLASMIDA PORTADORA DE UN ORIGEN DE REPLICACION, UNA SECUENCIA PROMOTORA INDUCIBLE, UNA SECUENCIA CODIFCADA DE DNA PARA UN PEPTIDO SEÑALADORA SECUENCIA CODIFICADA DE DNA PARA UNA APOLIPROTEINA AI-M Y UNA TERMINACION DE TRANSCRIPCION. EL INVENTO TAMBIEN HACE REFERENCIA AL METODO DE PRODUCIR LA APOLIPROTEINA AI-M UTILIZANDO EL SISTEMA DE EXPRESION.
ES94902173T 1992-12-11 1993-12-09 Sistema de expresion que permite producir una apoliproteina ai-m. Expired - Lifetime ES2146646T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE9203753A SE9203753D0 (sv) 1992-12-11 1992-12-11 Expression system for producing apolipoprotein ai-m

Publications (1)

Publication Number Publication Date
ES2146646T3 true ES2146646T3 (es) 2000-08-16

Family

ID=20388112

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94902173T Expired - Lifetime ES2146646T3 (es) 1992-12-11 1993-12-09 Sistema de expresion que permite producir una apoliproteina ai-m.

Country Status (22)

Country Link
US (1) US5721114A (es)
EP (1) EP0677109B1 (es)
JP (1) JP3899121B2 (es)
AT (1) ATE191930T1 (es)
AU (1) AU5663794A (es)
CA (1) CA2150928C (es)
CZ (1) CZ290865B6 (es)
DE (1) DE69328442T2 (es)
DK (1) DK0677109T3 (es)
ES (1) ES2146646T3 (es)
FI (1) FI952847A7 (es)
GR (1) GR3033966T3 (es)
HU (1) HU220193B (es)
IL (1) IL107896A (es)
MX (1) MX9307855A (es)
NO (1) NO319497B1 (es)
PL (1) PL175955B1 (es)
PT (1) PT677109E (es)
SE (1) SE9203753D0 (es)
SK (1) SK76695A3 (es)
WO (1) WO1994013819A1 (es)
ZA (1) ZA939266B (es)

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SE9603068D0 (sv) * 1996-08-23 1996-08-23 Pharmacia & Upjohn Ab Process for purifying a protein
SE9603303D0 (sv) 1996-09-11 1996-09-11 Pharmacia & Upjohn Ab Process for purifying a protein
SE9603304D0 (sv) 1996-09-11 1996-09-11 Pharmacia & Upjohn Ab Process for purifying a compound
US6306433B1 (en) 1997-08-12 2001-10-23 Pharmacia Ab Method of preparing pharmaceutical compositions
US6046166A (en) 1997-09-29 2000-04-04 Jean-Louis Dasseux Apolipoprotein A-I agonists and their use to treat dyslipidemic disorders
US6037323A (en) * 1997-09-29 2000-03-14 Jean-Louis Dasseux Apolipoprotein A-I agonists and their use to treat dyslipidemic disorders
US6004925A (en) 1997-09-29 1999-12-21 J. L. Dasseux Apolipoprotein A-I agonists and their use to treat dyslipidemic disorders
US6518412B1 (en) 1997-09-29 2003-02-11 Jean-Louis Dasseux Gene therapy approaches to supply apolipoprotein A-I agonists and their use to treat dyslipidemic disorders
CN1297932A (zh) * 1999-11-30 2001-06-06 上海博容基因开发有限公司 一种新的多肽——人脂蛋白前体蛋白信号肽11和编码这种多肽的多核苷酸
US20020064820A1 (en) * 2000-03-13 2002-05-30 Jean-Michel Dayer Apo-A-I regulation of T-cell signaling
JP4344136B2 (ja) * 2000-11-10 2009-10-14 エフ・ホフマン−ラ・ロシュ・リミテッド アポリポタンパク質類似体
US7217785B2 (en) * 2001-05-09 2007-05-15 The Regents Of The University Of California Cysteine-containing peptides having antioxidant properties
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US20030229062A1 (en) * 2001-12-07 2003-12-11 The Regents Of The University Of California Treatments for age-related macular degeneration (AMD)
WO2003049685A2 (en) * 2001-12-07 2003-06-19 The Regents Of The University Of California Treatment for age-related macular degeneration
US7470659B2 (en) * 2001-12-07 2008-12-30 The Regents Of The University Of California Methods to increase reverse cholesterol transport in the retinal pigment epithelium (RPE) and Bruch's membrane (BM)
US7223726B2 (en) * 2002-01-14 2007-05-29 The Regents Of The University Of California Apolipoprotein A-I mutant proteins having cysteine substitutions and polynucleotides encoding same
US20100204103A1 (en) * 2002-05-08 2010-08-12 The Regents Of The University Of California Helical synthetic peptides that stimulate cellular cholesterol efflux
US7691965B2 (en) * 2002-05-08 2010-04-06 The Regents Of The University Of California Helical synthetic peptides that stimulate cellular cholesterol efflux
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KR100560102B1 (ko) 2004-06-25 2006-03-13 한국생명공학연구원 프로아포리포단백질 ａ－ⅰ 변이체와, 이를 포함하는고지혈증 또는 동맥경화증 예방 및 치료제
CN1320119C (zh) * 2004-12-09 2007-06-06 复旦大学 人载脂蛋白ApoAⅠ在毕赤氏酵母胞内表达的方法
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Also Published As

Publication number Publication date
HUT71697A (en) 1996-01-29
MX9307855A (es) 1994-06-30
DE69328442D1 (de) 2000-05-25
PT677109E (pt) 2000-07-31
CZ148795A3 (en) 1996-01-17
CZ290865B6 (cs) 2002-11-13
PL175955B1 (pl) 1999-03-31
ZA939266B (en) 1994-08-08
IL107896A (en) 2004-06-01
FI952847L (fi) 1995-06-09
IL107896A0 (en) 1994-04-12
US5721114A (en) 1998-02-24
SE9203753D0 (sv) 1992-12-11
ATE191930T1 (de) 2000-05-15
SK76695A3 (en) 1996-02-07
NO319497B1 (no) 2005-08-22
NO952297L (no) 1995-06-09
WO1994013819A1 (en) 1994-06-23
CA2150928A1 (en) 1994-06-23
GR3033966T3 (en) 2000-11-30
NO952297D0 (no) 1995-06-09
EP0677109A1 (en) 1995-10-18
CA2150928C (en) 2005-02-08
JP3899121B2 (ja) 2007-03-28
JPH08506723A (ja) 1996-07-23
AU5663794A (en) 1994-07-04
PL309292A1 (en) 1995-10-02
FI952847A0 (fi) 1995-06-09
FI952847A7 (fi) 1995-06-09
DK0677109T3 (da) 2000-07-17
DE69328442T2 (de) 2000-09-07
HU220193B (hu) 2001-11-28
HU9501680D0 (en) 1995-08-28
EP0677109B1 (en) 2000-04-19

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