ES2156193T3 - Procedimiento, elemento de ensayo y equipo de ensayo para deteccion semicuantitativa de un acido nucleico diana. - Google Patents

Procedimiento, elemento de ensayo y equipo de ensayo para deteccion semicuantitativa de un acido nucleico diana.

Info

Publication number
ES2156193T3
ES2156193T3 ES95302151T ES95302151T ES2156193T3 ES 2156193 T3 ES2156193 T3 ES 2156193T3 ES 95302151 T ES95302151 T ES 95302151T ES 95302151 T ES95302151 T ES 95302151T ES 2156193 T3 ES2156193 T3 ES 2156193T3
Authority
ES
Spain
Prior art keywords
nucleic acid
detection
test element
semicuantitative
diana
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95302151T
Other languages
English (en)
Inventor
Lynn Bergmeyer
Thomas Cummins
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ortho Clinical Diagnostics Inc
Original Assignee
Johnson and Johnson Clinical Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson and Johnson Clinical Diagnostics Inc filed Critical Johnson and Johnson Clinical Diagnostics Inc
Application granted granted Critical
Publication of ES2156193T3 publication Critical patent/ES2156193T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

UN ACIDO NUCLEICO OBJETIVO PUEDE SER DETECTADO DE FORMA SEMICUANTITATIVA PASANDOLO SOBRE UNOS DEPOSITOS DETECTORES SITUADOS EN UN ELEMENTO DE PRUEBA. LOS DEPOSITOS DE DETECCION INCLUYEN PARTICULAS FIJADAS A LOS ELEMENTOS DE PRUEBA, ALGUNA DE DICHAS PARTICULAS CONTIENE PRUEBAS DE CAPTURA Y OTRAS PARTICULAS NO TIENEN PRUEBAS DE CAPTURA. LOS DEPOSITOS TIENEN CANTIDADES VARIADAS DE PRUEBAS CAPTORAS DE MANERA QUE LA SEÑAL OBTENIDA CUANDO EL OBJETIVO DE ACIDO NUCLEICO ES CAPTURADO PUEDE SER SEMICUANTITATIVAMENTE CORRELACIONADA CON LA CANTIDAD DE ACIDO NUCLEICO OBJETIVO EN EL ESPECIMEN. ESTE METODO DE DETECCION PUEDE SER USADO EN LOS ENSAYOS DE HIBRIDACION DE ACIDO NUCLEICO O SIGUIENTES METODOS DE AMPLIFICACION, INCLUYENDO CADENAS DE REACCION POLIMERASA.
ES95302151T 1994-03-31 1995-03-30 Procedimiento, elemento de ensayo y equipo de ensayo para deteccion semicuantitativa de un acido nucleico diana. Expired - Lifetime ES2156193T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/221,818 US5591580A (en) 1994-03-31 1994-03-31 Method, test element and test kit for semi-quantitative detection of target nucleic acid

Publications (1)

Publication Number Publication Date
ES2156193T3 true ES2156193T3 (es) 2001-06-16

Family

ID=22829523

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95302151T Expired - Lifetime ES2156193T3 (es) 1994-03-31 1995-03-30 Procedimiento, elemento de ensayo y equipo de ensayo para deteccion semicuantitativa de un acido nucleico diana.

Country Status (11)

Country Link
US (1) US5591580A (es)
EP (1) EP0677589B1 (es)
JP (1) JP3604758B2 (es)
AT (1) ATE201237T1 (es)
CA (1) CA2145957C (es)
DE (1) DE69520907T2 (es)
DK (1) DK0677589T3 (es)
ES (1) ES2156193T3 (es)
GR (1) GR3036316T3 (es)
PT (1) PT677589E (es)
SI (1) SI0677589T1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030148354A1 (en) * 1996-10-02 2003-08-07 Saftest, Inc. Devices and methods for isolating and detecting specific substances in complex matrices
EP2218731A1 (en) * 1997-11-28 2010-08-18 Merck Serono Biodevelopment Chlamydia trachomatis genomic sequence and polypeptides, fragments thereof and uses thereof, in particular for the diagnosis, prevention and treatment of infection
CA2321100A1 (en) * 1998-02-19 1999-08-26 Edvotek Articles of manufacture and methods for staining biomolecules
CA2470724A1 (en) * 2001-12-20 2003-07-03 Safetest, Inc. Devices and methods for isolating and detecting specific substances in complex matrices
US7217542B2 (en) * 2002-10-31 2007-05-15 Hewlett-Packard Development Company, L.P. Microfluidic system for analyzing nucleic acids
US6868912B2 (en) * 2003-02-19 2005-03-22 Baker Hughes Incorporated Tension thrust ESPCP system

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4711955A (en) * 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US4994373A (en) * 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
US5273882A (en) * 1985-06-13 1993-12-28 Amgen Method and kit for performing nucleic acid hybridization assays
CA1339723C (en) * 1988-01-19 1998-03-17 Philip Mcmahon Immunoassay with multiple test spots
EP0436547B1 (en) * 1988-05-10 1994-11-30 E.I. Du Pont De Nemours And Company Process for rapid nucleic acid detection
US5374524A (en) * 1988-05-10 1994-12-20 E. I. Du Pont De Nemours And Company Solution sandwich hybridization, capture and detection of amplified nucleic acids
GB8827160D0 (en) * 1988-11-21 1988-12-29 Apothekernes Lab Detection & quantitative determination of rna & dna
CA2002076A1 (en) * 1988-11-21 1990-05-21 Brent A. Burdick Diagnostic kit and method using a solid phase capture means for detecting nucleic acids
WO1990008840A1 (en) * 1989-02-03 1990-08-09 Eastman Kodak Company Nucleic acid test article and its use to detect a predetermined nucleic acid
CA2031659A1 (en) * 1990-01-26 1991-07-27 John B. Findlay Water-insoluble reagent, nucleic acid probe, test kit and diagnostic and purification methods
US5173260A (en) * 1990-09-17 1992-12-22 Eastman Kodak Company Beads fused to a test device support
US5380489A (en) * 1992-02-18 1995-01-10 Eastman Kodak Company Element and method for nucleic acid amplification and detection using adhered probes
JPH0830704B2 (ja) * 1991-03-21 1996-03-27 イーストマン コダック カンパニー 核酸増幅のための要素および方法ならびに付着プローブを使用する検出方法
US5387510A (en) * 1991-10-02 1995-02-07 Eastman Kodak Company Detection of amplified nucleic acid using secondary capture oligonucleotides and test kit

Also Published As

Publication number Publication date
SI0677589T1 (en) 2002-06-30
PT677589E (pt) 2001-08-30
DE69520907T2 (de) 2001-09-13
DK0677589T3 (da) 2001-07-30
US5591580A (en) 1997-01-07
CA2145957A1 (en) 1995-10-01
DE69520907D1 (de) 2001-06-21
EP0677589B1 (en) 2001-05-16
ATE201237T1 (de) 2001-06-15
GR3036316T3 (en) 2001-10-31
EP0677589A3 (en) 1998-01-07
EP0677589A2 (en) 1995-10-18
JPH0850130A (ja) 1996-02-20
JP3604758B2 (ja) 2004-12-22
CA2145957C (en) 2008-01-29

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