ES2165397T3 - Clonacion por complementacion y procesos relacionados. - Google Patents

Clonacion por complementacion y procesos relacionados.

Info

Publication number
ES2165397T3
ES2165397T3 ES95101715T ES95101715T ES2165397T3 ES 2165397 T3 ES2165397 T3 ES 2165397T3 ES 95101715 T ES95101715 T ES 95101715T ES 95101715 T ES95101715 T ES 95101715T ES 2165397 T3 ES2165397 T3 ES 2165397T3
Authority
ES
Spain
Prior art keywords
cloning
mammals
proteins
genes
products
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95101715T
Other languages
English (en)
Inventor
Michael H Wigler
John J Colicelli
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cold Spring Harbor Laboratory
Original Assignee
Cold Spring Harbor Laboratory
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cold Spring Harbor Laboratory filed Critical Cold Spring Harbor Laboratory
Application granted granted Critical
Publication of ES2165397T3 publication Critical patent/ES2165397T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/82—Translation products from oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Oncology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Geophysics And Detection Of Objects (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Details Of Aerials (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

SE DESCRIBEN METODOS PARA DETECTAR GENES DE MAMIFEROS CODIFICANDO PROTEINAS, QUE PUEDEN FUNCIONAR EN MICROORGANISMOS, ESPECIALMENTE FERMENTOS, PARA MODIFICAR, COMPLEMENTAR, O SUPRIMIR UN DEFECTO GENETICO RELACIONADO CON UNA ALTERACION O CARACTERISTICA FENOTIPICA IDENTIFICABLE EN EL MICROORGANISMO. TAMBIEN SE DESCRIBEN SECUENCIAS DEL DNA DE MAMIFEROS, CLONANDO, MEDIANTE EL ANTERIOR METODO, ASI COMO PRODUCTOS DE POLIPEPTIDOS DE LA EXPRESION DE LAS SECUENCIAS DEL DNA EN CELULAS HUESPEDES PROCARIOTICAS O EUCARIOTICAS, Y SUSTANCIAS ANTICUERPO, QUE SON ESPECIFICAMENTE INMUNORREACTIVAS CON LOS CITADOS PRODUCTOS DE EXPRESION. MAS ESPECIFICAMENTE, LA PRESENTE INVENCION SE REFIERE A METODOS PARA CLONAR GENES DE MAMIFEROS QUE CODIFICAN PRODUCTOS QUE MODIFICAN, COMPLEMENTAN O SUPRIMEN UN DEFECTO GENETICO EN UN PASO O VIA DE ACCESO BIOQUIMICO, EN EL CUAL CAMP PARTICIPA, O EN UN PASO BIOQUIMICO QUE SE CONTROLA, DIRECTA O INDIRECTAMENTE, MEDIANTE UNA PROTEINA RAS-RELACIONADA, A PRODUCTOS (RNA, PROTEINAS)CODIFICADOS POR LOS GENES DE MAMIFEROS CLONADOS DE ESTA FORMA, Y A ANTICUERPOS QUE PUEDEN UNIR LAS PROTEINAS CODIFICADAS.
ES95101715T 1990-04-20 1991-04-19 Clonacion por complementacion y procesos relacionados. Expired - Lifetime ES2165397T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/511,715 US6080540A (en) 1990-04-20 1990-04-20 Cloning of mammalian genes in microbial organisms and methods for pharmacological screening

Publications (1)

Publication Number Publication Date
ES2165397T3 true ES2165397T3 (es) 2002-03-16

Family

ID=24036135

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95101715T Expired - Lifetime ES2165397T3 (es) 1990-04-20 1991-04-19 Clonacion por complementacion y procesos relacionados.

Country Status (9)

Country Link
US (5) US6080540A (es)
EP (3) EP0940469A3 (es)
JP (3) JP4031027B2 (es)
AT (1) ATE204603T1 (es)
CA (1) CA2080920C (es)
DE (1) DE69132701T2 (es)
DK (1) DK0666314T3 (es)
ES (1) ES2165397T3 (es)
WO (1) WO1991016457A1 (es)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6080540A (en) * 1990-04-20 2000-06-27 Cold Spring Harbor Laboratory Cloning of mammalian genes in microbial organisms and methods for pharmacological screening
US6100025A (en) * 1990-04-20 2000-08-08 Cold Spring Harbor Laboratory Cloning by complementation and related processes
DE69233785D1 (de) * 1991-04-19 2010-07-08 Univ Washington diert
FR2689905B1 (fr) * 1992-04-13 1995-07-13 Orsan Procede de clonage d'une sequence d'adn codant pour une nadph cytochrome p450 reductase et sequences d'adn codant pour une nadph-cytochrome p450 reductase, notamment de plantes.
DE69435280D1 (de) * 1993-03-10 2010-04-15 Glaxosmithkline Llc Phosphodiesterase des menschlichen Gehirns und Screening-Verfahren
US6323041B1 (en) * 1993-06-11 2001-11-27 Pfizer Inc. Screening novel human phosphodiesterase IV isozymes for compounds which modify their enzymatic activity
US5672509A (en) * 1993-08-25 1997-09-30 Pfizer Inc. hPDE IV-C: a human phosphodiesterase IV isozyme
US6069240A (en) * 1994-03-01 2000-05-30 Icos Corporation Cloning by complementation and related processes
US6015880A (en) * 1994-03-16 2000-01-18 California Institute Of Technology Method and substrate for performing multiple sequential reactions on a matrix
US6558633B1 (en) 1994-09-21 2003-05-06 Isis Pharmaceuticals, Inc. Chemical reaction apparatus and methods
US5871945A (en) * 1994-11-23 1999-02-16 Icos Corporation Modulators of anchoring protein function
GB2301363B (en) * 1994-12-23 1998-12-30 Celltech Therapeutics Ltd Human phosphodiesterase type ivc and its production and use
KR100501838B1 (ko) * 1995-04-24 2005-07-21 큐비스트 파마슈티컬즈 인코포레이티드 새로운 대사 경로를 만들고 이를 스크리닝하는 방법
US5795735A (en) * 1995-07-17 1998-08-18 Icos Corporation Isolated polynucleotides encoding PKA-binding proteins and methods of producing the proteins recombinantly
US6242211B1 (en) 1996-04-24 2001-06-05 Terragen Discovery, Inc. Methods for generating and screening novel metabolic pathways
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US5958342A (en) * 1996-05-17 1999-09-28 Incyte Pharmaceuticals, Inc. Jet droplet device
AU6956798A (en) * 1997-04-10 1998-10-30 Genetics Institute Inc. Secreted expressed sequence tags (sests)
AU9315698A (en) 1997-09-11 1999-03-29 Beth Israel Deaconess Medical Center (rin2), a novel inhibitor of ras-mediated signaling
US5965707A (en) * 1997-09-11 1999-10-12 Beth Israel Deaconess Medical Center Rin2, a novel inhibitor of Ras-mediated signaling
US20060009633A9 (en) * 1997-11-13 2006-01-12 Genset, S.A. Complementary DNAs encoding proteins with signal peptides
US20060286639A1 (en) * 1997-11-13 2006-12-21 Serono Genetics Institute S.A. Complementary DNAs encoding proteins with signal peptides
US6548633B1 (en) 1998-12-22 2003-04-15 Genset, S.A. Complementary DNA's encoding proteins with signal peptides
DE19822287C2 (de) * 1998-05-18 2003-04-24 Switch Biotech Ag Klonierungsvektor, seine Herstellung und Verwendung zur Analyse von mRNA Expressionsmuster
US6506889B1 (en) * 1998-05-19 2003-01-14 University Technology Corporation Ras suppressor SUR-8 and related compositions and methods
DK1018559T3 (da) 1998-12-23 2007-12-03 Pfizer Phosphodiesteraser
AU764005B2 (en) 1999-02-25 2003-08-07 Merck Frosst Canada & Co. PDE IV inhibiting compounds, compositions and methods of treatment
US7098015B2 (en) * 1999-06-11 2006-08-29 Millennium Pharmaceuticals, Inc. 27875, 22025, 27420, 17906, 16319, 55092 and 10218 molecules and uses therefor
US6146876A (en) * 1999-06-11 2000-11-14 Millennium Pharmaceuticals, Inc. 22025, a novel human cyclic nucleotide phosphodiesterase
CA2395872A1 (en) * 2000-01-31 2001-08-02 Human Genome Sciences, Inc. Nucleic acids, proteins, and antibodies
WO2001083772A1 (en) * 2000-04-28 2001-11-08 Merck Patent Gmbh Splice variant of camp phosphodiesterase type 7 (pde7a3)
US6958214B2 (en) 2000-07-10 2005-10-25 Sequenom, Inc. Polymorphic kinase anchor proteins and nucleic acids encoding the same
AU784975B2 (en) * 2000-10-11 2006-08-10 Sumitomo Chemical Company, Limited DNA-binding protein YB-1-containing collagen accumulation inhibitors
US6479493B1 (en) 2001-08-23 2002-11-12 Cell Pathways, Inc. Methods for treatment of type I diabetes
AU2003228809A1 (en) 2002-05-03 2003-11-17 Sequenom, Inc. Kinase anchor protein muteins, peptides thereof, and related methods
US7109224B2 (en) * 2002-11-06 2006-09-19 Bristol-Myers Squibb Co. Acyl guanidine compounds and use thereof
AU2003291310A1 (en) * 2002-11-06 2004-06-03 Bristol-Myers Squibb Company Fused heterocyclic compounds and use thereof
US20070172896A1 (en) * 2005-12-06 2007-07-26 Promega Corporation Methods for cyclic nucleotide determination
WO2008022153A2 (en) * 2006-08-14 2008-02-21 The Regents Of The University Of California Inhibitors of pde4 and methods of use
US9677139B2 (en) 2009-12-11 2017-06-13 Cold Spring Harbor Laboratory Genetic markers indicative of a cancer patient response to trastuzumab (herceptin)
HRP20182039T1 (hr) 2010-04-23 2019-02-08 Cold Spring Harbor Laboratory NOVE STRUKTURNO DIZAJNIRANE MOLEKULE shRNA
CA3080686C (en) 2010-10-22 2023-10-10 Cold Spring Harbor Laboratory Varietal counting of nucleic acids for obtaining genomic copy number information
WO2017139959A1 (zh) * 2016-02-18 2017-08-24 天津大学 一种高产cAMP的酵母菌株及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4861709A (en) * 1985-05-31 1989-08-29 Technicon Research A.G. Detection and/or identification of microorganisms in a test sample using bioluminescence or other exogenous genetically-introduced marker
AU7512287A (en) * 1986-05-20 1987-12-22 Dana-Farber Cancer Institute Expression of human t-cell lymphotropic virus (htlv-iii)reve rse transcriptase and uses thereof
US4980281A (en) * 1988-02-10 1990-12-25 Housey Gerard M Method of screening for protein inhibitors and activators
US6080540A (en) * 1990-04-20 2000-06-27 Cold Spring Harbor Laboratory Cloning of mammalian genes in microbial organisms and methods for pharmacological screening
IE20020110A1 (en) 1990-09-13 2003-05-28 Univ Duke Expression of G Protein Coupled Receptors in Yeast

Also Published As

Publication number Publication date
EP0666314B1 (en) 2001-08-22
DK0666314T3 (da) 2001-12-10
DE69132701D1 (de) 2001-09-27
CA2080920A1 (en) 1991-10-21
JP3258635B2 (ja) 2002-02-18
JPH11253200A (ja) 1999-09-21
US5527896A (en) 1996-06-18
EP0537173A4 (en) 1993-12-22
ATE204603T1 (de) 2001-09-15
US20040086900A1 (en) 2004-05-06
EP0537173A1 (en) 1993-04-21
US6569617B1 (en) 2003-05-27
EP0940469A2 (en) 1999-09-08
HK1013314A1 (en) 1999-08-20
US20060199247A1 (en) 2006-09-07
EP0940469A3 (en) 1999-12-22
CA2080920C (en) 2003-07-08
JPH11239481A (ja) 1999-09-07
EP0666314A1 (en) 1995-08-09
US6080540A (en) 2000-06-27
DE69132701T2 (de) 2002-06-20
JPH05507196A (ja) 1993-10-21
WO1991016457A1 (en) 1991-10-31
JP4031027B2 (ja) 2008-01-09

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