ES2172887T3 - Un metodo para caracterizar moleculas de acido nucleico que implica generar fragmentos de dna aguas arriba y extensibles que resultan de la escision de un acido nucleico en un sitio abasico. - Google Patents

Un metodo para caracterizar moleculas de acido nucleico que implica generar fragmentos de dna aguas arriba y extensibles que resultan de la escision de un acido nucleico en un sitio abasico.

Info

Publication number
ES2172887T3
ES2172887T3 ES98917568T ES98917568T ES2172887T3 ES 2172887 T3 ES2172887 T3 ES 2172887T3 ES 98917568 T ES98917568 T ES 98917568T ES 98917568 T ES98917568 T ES 98917568T ES 2172887 T3 ES2172887 T3 ES 2172887T3
Authority
ES
Spain
Prior art keywords
nucleic acid
site
extensible
abasical
excision
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES98917568T
Other languages
English (en)
Inventor
Thomas Valentine Mccarthy
Patrick Martin Vaughan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ENTPR IE TRD AS BIORESEARCH IE
University College Cork
Enterprise Ireland
Original Assignee
ENTPR IE TRD AS BIORESEARCH IE
University College Cork
Enterprise Ireland
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Filing date
Publication date
Application filed by ENTPR IE TRD AS BIORESEARCH IE, University College Cork, Enterprise Ireland filed Critical ENTPR IE TRD AS BIORESEARCH IE
Application granted granted Critical
Publication of ES2172887T3 publication Critical patent/ES2172887T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Un método para caracterizar moléculas de ácido nucleico, que comprende las etapas de: I) introducir una base modificada que es un sustrato de una DNA-glicosilasa en una molécula de DNA; II) cortar la base modificada por medio de dicha DNA-glicosilasa de manera que se genera un sitio abásico; III) escindir el DNA en el sitio abásico de manera que se genera un fragmento de DNA aguas arriba que puede ser extendido; y IV) incubar el fragmento aguas arriba y extensible en presencia de una enzima que permite su extensión y de un ácido nucleico molde, y analizar el fragmento(s) resultante.
ES98917568T 1998-04-22 1998-04-22 Un metodo para caracterizar moleculas de acido nucleico que implica generar fragmentos de dna aguas arriba y extensibles que resultan de la escision de un acido nucleico en un sitio abasico. Expired - Lifetime ES2172887T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IE1998/000030 WO1999054501A1 (en) 1998-04-22 1998-04-22 A method for the characterisation of nucleic acid molecules involving generation of extendible upstream dna fragments resulting from the cleavage of nucleic acid at an abasic site

Publications (1)

Publication Number Publication Date
ES2172887T3 true ES2172887T3 (es) 2002-10-01

Family

ID=11042510

Family Applications (1)

Application Number Title Priority Date Filing Date
ES98917568T Expired - Lifetime ES2172887T3 (es) 1998-04-22 1998-04-22 Un metodo para caracterizar moleculas de acido nucleico que implica generar fragmentos de dna aguas arriba y extensibles que resultan de la escision de un acido nucleico en un sitio abasico.

Country Status (14)

Country Link
US (1) US7175982B1 (es)
EP (1) EP1071811B8 (es)
JP (1) JP2002512044A (es)
CN (1) CN1198943C (es)
AT (1) ATE214104T1 (es)
AU (1) AU753661B2 (es)
CA (1) CA2325854C (es)
DE (1) DE69804143T2 (es)
DK (1) DK1071811T3 (es)
ES (1) ES2172887T3 (es)
NO (1) NO20005311L (es)
NZ (1) NZ508358A (es)
PT (1) PT1071811E (es)
WO (1) WO1999054501A1 (es)

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US20030190644A1 (en) 1999-10-13 2003-10-09 Andreas Braun Methods for generating databases and databases for identifying polymorphic genetic markers
US6958214B2 (en) 2000-07-10 2005-10-25 Sequenom, Inc. Polymorphic kinase anchor proteins and nucleic acids encoding the same
IE20000887A1 (en) * 2000-11-03 2002-12-11 Univ College Cork Nat Univ Ie Method for the amplification and optional characterisation of nucleic acids
GB0126887D0 (en) * 2001-11-08 2002-01-02 Univ London Method for producing and identifying soluble protein domains
CA2476481C (en) 2002-02-21 2016-01-26 Asm Scientific, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7435572B2 (en) * 2002-04-12 2008-10-14 New England Biolabs, Inc. Methods and compositions for DNA manipulation
US7432342B2 (en) 2002-05-03 2008-10-07 Sequenom, Inc. Kinase anchor protein muteins, peptides thereof and related documents
IE20020544A1 (en) * 2002-06-28 2003-12-31 Univ College Cork Nat Univ Ie Method for the characterisation of nucleic acid molecules
CA2494993A1 (en) 2002-08-21 2004-03-04 Epoch Biosciences, Inc. Abasic site endonuclease assay
CA2507189C (en) 2002-11-27 2018-06-12 Sequenom, Inc. Fragmentation-based methods and systems for sequence variation detection and discovery
KR100683025B1 (ko) * 2003-06-30 2007-02-15 마츠시타 덴끼 산교 가부시키가이샤 뉴클레오티드쇄 수식방법
CA2552007A1 (en) 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
US7608394B2 (en) 2004-03-26 2009-10-27 Sequenom, Inc. Methods and compositions for phenotype identification based on nucleic acid methylation
US9249456B2 (en) 2004-03-26 2016-02-02 Agena Bioscience, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
JP2006271311A (ja) * 2005-03-30 2006-10-12 Hitachi Ltd 核酸分析方法
EP1929049B1 (en) * 2005-07-25 2013-04-10 Alere San Diego, Inc. Methods for multiplexing recombinase polymerase amplification
JP2009535053A (ja) 2006-05-04 2009-10-01 エーエスエム サイエンティフィック， インコーポレイテッド レコンビナーゼポリメラーゼ増幅
EP2152915A2 (en) * 2007-05-14 2010-02-17 Insight Genetics, Inc. Methods of screening nucleic acids for single nucleotide variations
US20100285537A1 (en) * 2009-04-02 2010-11-11 Fluidigm Corporation Selective tagging of short nucleic acid fragments and selective protection of target sequences from degradation
US9469867B2 (en) 2009-05-20 2016-10-18 Alere San Diego, Inc. DNA glycosylase/lyase and AP endonuclease substrates
EP2480685B1 (en) 2009-09-23 2013-08-28 Koninklijke Philips Electronics N.V. Method for monitoring the bisulfite-mediated conversion of dna
AU2011372509B2 (en) * 2011-07-01 2017-02-09 Htg Molecular Diagnostics, Inc. Methods of detecting gene fusions
SG10201510189WA (en) 2011-10-19 2016-01-28 Nugen Technologies Inc Compositions And Methods For Directional Nucleic Acid Amplification And Sequencing
SG11201404243WA (en) 2012-01-26 2014-08-28 Nugen Technologies Inc Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
JP6181751B2 (ja) 2012-06-18 2017-08-16 ニューゲン テクノロジーズ， インコーポレイテッド 望まれない核酸配列のネガティブ選択のための組成物および方法
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
CA2929596C (en) 2013-11-13 2022-07-05 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
US10266828B2 (en) 2013-12-16 2019-04-23 Syddansk Universitet RAS exon 2 skipping for cancer treatment
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
CN107075581B (zh) 2014-08-06 2022-03-18 纽亘技术公司 由靶向测序进行数字测量
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
EP3851542A1 (en) 2020-01-20 2021-07-21 Tecan Genomics, Inc. Depletion of abundant uninformative sequences
CN113275053A (zh) 2020-02-03 2021-08-20 帝肯基因组学公司 试剂存储系统

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996030545A1 (en) * 1995-03-24 1996-10-03 Mitokor Mutation detection by differential primer extension of mutant and wildtype target sequences
US5656430A (en) * 1995-06-07 1997-08-12 Trevigen, Inc. Oscillating signal amplifier for nucleic acid detection
JP3790797B2 (ja) 1995-07-11 2006-06-28 フォーファス グリコシラーゼによる候補座位のヌクレオチド配列の検出
US6190865B1 (en) 1995-09-27 2001-02-20 Epicentre Technologies Corporation Method for characterizing nucleic acid molecules

Also Published As

Publication number Publication date
DE69804143T2 (de) 2002-08-01
US7175982B1 (en) 2007-02-13
DK1071811T3 (da) 2002-06-17
NO20005311D0 (no) 2000-10-20
EP1071811B1 (en) 2002-03-06
WO1999054501A1 (en) 1999-10-28
PT1071811E (pt) 2002-08-30
DE69804143D1 (de) 2002-04-11
AU7075098A (en) 1999-11-08
JP2002512044A (ja) 2002-04-23
CA2325854A1 (en) 1999-10-28
CA2325854C (en) 2005-12-06
AU753661B2 (en) 2002-10-24
CN1291240A (zh) 2001-04-11
EP1071811B8 (en) 2003-01-02
NZ508358A (en) 2002-07-26
NO20005311L (no) 2000-12-21
EP1071811A1 (en) 2001-01-31
ATE214104T1 (de) 2002-03-15
CN1198943C (zh) 2005-04-27

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