ES2270460T3 - Un proceso para producir un polipeptido recombinante que involucra la adicion al medio cultivo celular de un inhibidor de proteasas o quimiotripsoinas dependientes de metales. - Google Patents

Un proceso para producir un polipeptido recombinante que involucra la adicion al medio cultivo celular de un inhibidor de proteasas o quimiotripsoinas dependientes de metales. Download PDF

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Publication number: ES2270460T3
Authority: ES; Spain
Prior art keywords: factor viii; culture medium; process according; metal; cell culture
Prior art date: 1996-05-14
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

ES97923382T

Other languages

English (en)

Spanish (es)

Inventor

Lars Adamson

Erik Walum

Johan Dixelius

Kristina Lima Lie

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Swedish Orphan Biovitrum AB

Original Assignee

Biovitrum AB

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1996-05-14

Filing date

1997-05-13

Publication date

2007-04-01

1996-05-14 Priority claimed from SE9601855A external-priority patent/SE9601855D0/xx

1997-05-13 Application filed by Biovitrum AB filed Critical Biovitrum AB

2007-04-01 Application granted granted Critical

2007-04-01 Publication of ES2270460T3 publication Critical patent/ES2270460T3/es

2017-05-13 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

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125000000623 heterocyclic group Chemical group 0.000 description 1
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239000012510 hollow fiber Substances 0.000 description 1
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238000000338 in vitro Methods 0.000 description 1
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210000003093 intracellular space Anatomy 0.000 description 1
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229940039781 leptin Drugs 0.000 description 1
NRYBAZVQPHGZNS-ZSOCWYAHSA-N leptin Chemical compound O=C([C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](N)CC(C)C)CCSC)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CS)C(O)=O NRYBAZVQPHGZNS-ZSOCWYAHSA-N 0.000 description 1
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238000004264 monolayer culture Methods 0.000 description 1
239000006225 natural substrate Substances 0.000 description 1
230000014508 negative regulation of coagulation Effects 0.000 description 1
235000015097 nutrients Nutrition 0.000 description 1
235000016709 nutrition Nutrition 0.000 description 1
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SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 description 1
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UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
108020003175 receptors Proteins 0.000 description 1
102000005962 receptors Human genes 0.000 description 1
230000001105 regulatory effect Effects 0.000 description 1
239000006152 selective media Substances 0.000 description 1
229960001471 sodium selenite Drugs 0.000 description 1
235000015921 sodium selenite Nutrition 0.000 description 1
239000011781 sodium selenite Substances 0.000 description 1
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238000010186 staining Methods 0.000 description 1
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108700012359 toxins Proteins 0.000 description 1
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239000011712 vitamin K Substances 0.000 description 1
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229940046010 vitamin k Drugs 0.000 description 1
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238000001262 western blot Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
General Health & Medical Sciences (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
Molecular Biology (AREA)
General Engineering & Computer Science (AREA)
Biochemistry (AREA)
Medicinal Chemistry (AREA)
Hematology (AREA)
Microbiology (AREA)
General Chemical & Material Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Biomedical Technology (AREA)
Biophysics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Toxicology (AREA)
Plant Pathology (AREA)
Veterinary Medicine (AREA)
Public Health (AREA)
Animal Behavior & Ethology (AREA)
Pharmacology & Pharmacy (AREA)
Physics & Mathematics (AREA)
Gastroenterology & Hepatology (AREA)
Diabetes (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Peptides Or Proteins (AREA)
Enzymes And Modification Thereof (AREA)

ES97923382T 1996-05-14 1997-05-13 Un proceso para producir un polipeptido recombinante que involucra la adicion al medio cultivo celular de un inhibidor de proteasas o quimiotripsoinas dependientes de metales. Expired - Lifetime ES2270460T3 (es)

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date	Title
SE9601855		1996-05-14
SE9601855A SE9601855D0 (sv)	1996-05-14	1996-05-14	Process for producing a protein
US1887496P	1996-05-29	1996-05-29
US18874P		1996-05-29

Publications (1)

Publication Number	Publication Date
ES2270460T3 true ES2270460T3 (es)	2007-04-01

Family

ID=26662616

Family Applications (2)

Application Number	Title	Priority Date	Filing Date
ES97923382T Expired - Lifetime ES2270460T3 (es)	1996-05-14	1997-05-13	Un proceso para producir un polipeptido recombinante que involucra la adicion al medio cultivo celular de un inhibidor de proteasas o quimiotripsoinas dependientes de metales.
ES03000391T Expired - Lifetime ES2260524T3 (es)	1996-05-14	1997-05-13	Procedimiento para la produccion de un polipeptido recombinante que involucra la adicion de un inhibidor de quimotripsina en el medio del cultivo celular.

Family Applications After (1)

Application Number	Title	Priority Date	Filing Date
ES03000391T Expired - Lifetime ES2260524T3 (es)	1996-05-14	1997-05-13	Procedimiento para la produccion de un polipeptido recombinante que involucra la adicion de un inhibidor de quimotripsina en el medio del cultivo celular.

Country Status (13)

Country	Link
US (1)	US5851800A (2)
EP (1)	EP0934424B1 (2)
JP (2)	JP2000509994A (2)
AT (2)	ATE321141T1 (2)
AU (1)	AU716245B2 (2)
CA (1)	CA2253287C (2)
DE (2)	DE69735510T2 (2)
DK (1)	DK0934424T3 (2)
ES (2)	ES2270460T3 (2)
NO (2)	NO325358B1 (2)
NZ (1)	NZ332751A (2)
PT (2)	PT1318198E (2)
WO (1)	WO1997043436A1 (2)

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US6475725B1 (en) *	1997-06-20	2002-11-05	Baxter Aktiengesellschaft	Recombinant cell clones having increased stability and methods of making and using the same
EP2921180B1 (en)	1999-02-22	2019-08-14	University of Connecticut	Albumin-free factor VIII formulations
AT409379B (de)	1999-06-02	2002-07-25	Baxter Ag	Medium zur protein- und serumfreien kultivierung von zellen
ATE330003T1 (de) *	1999-08-05	2006-07-15	Baxter Ag	Rekombinanter stabiler zellklon, seine herstellung und verwendung
WO2001052891A1 (fr) *	2000-01-20	2001-07-26	Nippon Organon K. K.	Inhibiteurs d'invasion plasmodiale dans des erythrocytes
PT2287288E (pt) *	2002-07-09	2012-12-10	Baxter Int	Meio isento de proteína animal para cultura de células
CN101857851A (zh) *	2002-12-23	2010-10-13	布里斯托尔-迈尔斯斯奎布公司	用于蛋白质生产的哺乳动物细胞培养方法
DE60335024D1 (de) *	2002-12-23	2010-12-30	Bristol Myers Squibb Co	Produktqualitätsverbesserung in säugerzellkulturverfahrenzur proteinproduktion
US20040265964A1 (en) *	2003-04-25	2004-12-30	Martin Allen	Inducers of recombinant protein expression
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EP1707634A1 (en) *	2005-03-29	2006-10-04	Octapharma AG	Method for isolation of recombinantly produced proteins
EP3653699A1 (en)	2006-01-04	2020-05-20	Baxalta Incorporated	Oligopeptide-free cell culture media
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EP1988101A1 (en)	2007-05-04	2008-11-05	Novo Nordisk A/S	Improvement of factor VIII polypeptide titers in cell cultures
WO2008135498A2 (en) *	2007-05-04	2008-11-13	Novo Nordisk A/S	Prevention of protein degradation in mammalian cell cultures
NZ593190A (en)	2008-11-07	2013-01-25	Baxter Int	Factor viii formulations
US9540426B2 (en)	2009-10-06	2017-01-10	Bristol-Myers Squibb Company	Mammalian cell culture processes for protein production
CN107190034A (zh)	2010-10-05	2017-09-22	诺沃—诺迪斯克保健股份有限公司	生产蛋白质的方法
EP2906683B1 (en)	2012-10-15	2017-05-31	Bristol-Myers Squibb Company	Mammalian cell culture processes for protein production
KR102668200B1 (ko)	2015-10-28	2024-05-23	주식회사유한양행	지속형 fgf21 융합 단백질 및 이를 포함하는 약학적 조성물
KR102670157B1 (ko)	2015-10-28	2024-05-29	주식회사유한양행	이중 작용 단백질 및 이를 포함하는 약학적 조성물
AU2017358289C1 (en)	2016-11-10	2025-05-08	Yuhan Corporation	Pharmaceutical composition for preventing or treating hepatitis, hepatic fibrosis, and hepatic cirrhosis comprising fusion proteins
CN110709520B (zh)	2017-04-21	2024-04-05	株式会社柳韩洋行	用于产生双功能蛋白质及其衍生物的方法

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US5543396A (en) *	1994-04-28	1996-08-06	Georgia Tech Research Corp.	Proline phosphonate derivatives

1997
- 1997-05-07 US US08/852,783 patent/US5851800A/en not_active Expired - Lifetime
- 1997-05-13 JP JP09540800A patent/JP2000509994A/ja active Pending
- 1997-05-13 DE DE69735510T patent/DE69735510T2/de not_active Expired - Lifetime
- 1997-05-13 WO PCT/SE1997/000783 patent/WO1997043436A1/en not_active Ceased
- 1997-05-13 AT AT03000391T patent/ATE321141T1/de active
- 1997-05-13 NZ NZ332751A patent/NZ332751A/en not_active IP Right Cessation
- 1997-05-13 AT AT97923382T patent/ATE334223T1/de active
- 1997-05-13 AU AU29197/97A patent/AU716245B2/en not_active Ceased
- 1997-05-13 PT PT03000391T patent/PT1318198E/pt unknown
- 1997-05-13 DK DK97923382T patent/DK0934424T3/da active
- 1997-05-13 EP EP97923382A patent/EP0934424B1/en not_active Expired - Lifetime
- 1997-05-13 PT PT97923382T patent/PT934424E/pt unknown
- 1997-05-13 CA CA002253287A patent/CA2253287C/en not_active Expired - Fee Related
- 1997-05-13 DE DE69736391T patent/DE69736391T2/de not_active Expired - Lifetime
- 1997-05-13 ES ES97923382T patent/ES2270460T3/es not_active Expired - Lifetime
- 1997-05-13 ES ES03000391T patent/ES2260524T3/es not_active Expired - Lifetime
1998
- 1998-11-13 NO NO19985297A patent/NO325358B1/no not_active IP Right Cessation
2006
- 2006-11-02 NO NO20065027A patent/NO325633B1/no not_active IP Right Cessation
2009
- 2009-01-07 JP JP2009001597A patent/JP4896999B2/ja not_active Expired - Fee Related

Also Published As

Publication number	Publication date
CA2253287A1 (en)	1997-11-20
WO1997043436A1 (en)	1997-11-20
JP4896999B2 (ja)	2012-03-14
EP0934424B1 (en)	2006-07-26
DE69736391T2 (de)	2007-08-16
DE69735510T2 (de)	2006-08-24
AU716245B2 (en)	2000-02-24
CA2253287C (en)	2008-02-19
JP2000509994A (ja)	2000-08-08
NO20065027L (no)	1999-01-14
EP0934424A1 (en)	1999-08-11
PT934424E (pt)	2006-12-29
US5851800A (en)	1998-12-22
DE69735510D1 (de)	2006-05-11
DK0934424T3 (da)	2006-11-20
ATE321141T1 (de)	2006-04-15
NO985297L (no)	1999-01-14
NO325633B1 (no)	2008-06-30
ATE334223T1 (de)	2006-08-15
AU2919797A (en)	1997-12-05
DE69736391D1 (de)	2006-09-07
NO985297D0 (no)	1998-11-13
ES2260524T3 (es)	2006-11-01
PT1318198E (pt)	2006-08-31
NO325358B1 (no)	2008-04-07
JP2009148271A (ja)	2009-07-09
NZ332751A (en)	2000-06-23

Publication	Publication Date	Title
ES2270460T3 (es)	2007-04-01	Un proceso para producir un polipeptido recombinante que involucra la adicion al medio cultivo celular de un inhibidor de proteasas o quimiotripsoinas dependientes de metales.
KR102007773B1 (ko)	2019-08-06	세포 배양에서 재조합 고분자량 ｖＷＦ를 생산하는 방법
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