ES2548158T3 - Aparato de procesamiento de portaobjetos automático - Google Patents

Aparato de procesamiento de portaobjetos automático Download PDF

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Publication number
ES2548158T3
ES2548158T3 ES11002712.5T ES11002712T ES2548158T3 ES 2548158 T3 ES2548158 T3 ES 2548158T3 ES 11002712 T ES11002712 T ES 11002712T ES 2548158 T3 ES2548158 T3 ES 2548158T3
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Spain
Prior art keywords
holder
reagent
slide
nozzle
slide processing
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Expired - Lifetime
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ES11002712.5T
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English (en)
Inventor
Charles Dr. Lemme
David Bryant
Devon C. Campbell
Andrew Ghusson
Kurt Reinhardt
William Richards
Vincent Rizzo
Wayne Showalter
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Ventana Medical Systems Inc
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Ventana Medical Systems Inc
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G01N1/312—Apparatus therefor for samples mounted on planar substrates
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y10T436/111666—Utilizing a centrifuge or compartmented rotor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y10T436/112499—Automated chemical analysis with sample on test slide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y10T436/115831—Condition or time responsive
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Un aparato de procesamiento de portaobjetos automático que comprende: - un soporte de portaobjetos (1033) - un soporte de reactivos montado de manera rotatoria (1300) para sostener una pluralidad de dispensadores de reactivos (1302); - un soporte de boquilla (1100) para sostener unas estaciones de tratamiento de portaobjetos (1106); y - un activador de dispensador de reactivos (1400) para activar de manera selectiva los dispensadores de reactivos respectivos (1302), caracterizado por que el soporte de portaobjetos (1033), durante el funcionamiento del aparato de procesamiento de portaobjetos, se fija en su posición, mientras que el soporte de boquilla (1100) y el soporte de reactivos (1300) pueden girar de manera selectiva e independiente en relación con el soporte de portaobjetos (1033), y en el que el activador de dispensador de reactivos (1400) se monta para girar en sincronización con el soporte de boquilla (1100).

Description

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E11002712
23-09-2015
dispensador, es decir, como se explica en detalle en la patente de Estados Unidos 6.192.945. Por lo tanto, con el fin de dispensar un reactivo seleccionado en un portaobjetos seleccionado, el soporte de boquilla 1110 se mueve de manera rotatoria a una posición de dispensación verticalmente sobre el portaobjetos seleccionado que se había preparado previamente, por ejemplo, lavado, etc., por las estaciones de tratamiento sostenidas en el soporte de
5 boquilla 1100. Puesto que el martillo está enchavetado para moverse con el soporte de boquilla 1100, no se pierde tiempo en el movimiento del martillo o pistón, y el martillo o pistón se alinea verticalmente. Una característica y ventaja específica de la presente invención es que el soporte de reactivos 1300 no requiere ni conexiones eléctricas ni de tuberías. Por lo tanto, el soporte de reactivos 1300 es libre de girar en cualquier dirección, sin limitaciones.
10 Haciendo referencia a las figuras 19A y 19B, el proceso general es el siguiente: una pluralidad de portaobjetos de apoyo de muestra 1060 se montan en las plataformas de portaobjetos 1050, y los dispensadores de reactivos seleccionados 1302 se montan en el soporte de reactivos 1300. Se cierra el cajón de portaobjetos y se leen los códigos de barras del portaobjetos y del reactivo. El ordenador calcula el protocolo maestro y, a continuación, descarga las etapas de ejecución para toda la ejecución, el soporte de boquilla 1100 y el martillo o pistón 1400 se
15 indexan en el primer portaobjetos, y el portaobjetos se lava y se prepara para la tinción u otro tratamiento de acuerdo con las etapas de ejecución preprogramadas haciendo avanzar el soporte de boquilla 1100 en la manera de “paso con bloqueo”. Mientras tanto, se hace girar el soporte de reactivos 1300 para localizar un dispensador de reactivos seleccionado 1302 sobre el portaobjetos seleccionado 1060, y el martillo o pistón 1400 activado para dispensar una cantidad medida del reactivo deseado. El proceso se repite para un segundo portaobjetos seleccionado, y así
20 sucesivamente.
11

Claims (1)

  1. imagen1
ES11002712.5T 2002-04-26 2003-04-28 Aparato de procesamiento de portaobjetos automático Expired - Lifetime ES2548158T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37567902P 2002-04-26 2002-04-26
US375679P 2002-04-26

Publications (1)

Publication Number Publication Date
ES2548158T3 true ES2548158T3 (es) 2015-10-14

Family

ID=29270685

Family Applications (2)

Application Number Title Priority Date Filing Date
ES03726474.4T Expired - Lifetime ES2556242T3 (es) 2002-04-26 2003-04-28 Aparato de patología molecular automatizado que tiene plataformas de portaobjetos fijas
ES11002712.5T Expired - Lifetime ES2548158T3 (es) 2002-04-26 2003-04-28 Aparato de procesamiento de portaobjetos automático

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES03726474.4T Expired - Lifetime ES2556242T3 (es) 2002-04-26 2003-04-28 Aparato de patología molecular automatizado que tiene plataformas de portaobjetos fijas

Country Status (9)

Country Link
US (3) US7378055B2 (es)
EP (2) EP1499872B1 (es)
JP (1) JP4091599B2 (es)
CN (1) CN1650159A (es)
AU (1) AU2003228709B2 (es)
CA (1) CA2482763C (es)
DK (2) DK1499872T3 (es)
ES (2) ES2556242T3 (es)
WO (1) WO2003091710A1 (es)

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